Table Of Content

1 Table of Contents Introduction .................................................................................................................................. 24 Executive Summary ...................................................................................................................... 29 Biology and Biotechnology .......................................................................................................... 33 Animal Biology - Invertebrates Drosophila Motility, Behaviour and Ageing (Ageing) .......................................................................... 34 Study of the Resistance of a Modeled Closed Ecosystem and Chains of its Components in Microgravity (Akvarium) ..................................................................................................................... 36 Caenorhabditis elegans RNAi Space Experiment (CERISE) .................................................................. 39 Commercial Generic Bioprocessing Apparatus (CGBA), Three Investigations .................................... 41 Crickets in Space-2 (CRISP-2) .............................................................................................................. 44 Fungal Pathogenesis, Tumorigenesis, and Effects of Hose Immunity in Space (FIT) .......................... 46 International Caenorhabditis Elegans Experiment First Flight (ICE-First), Eight Investigations ......... 48 NanoRacks-University of Florida-1 (NanoRacks-UF-Squids-1) ............................................................ 55 Integrated Assessment of Long-term Cosmic Radiation Through Biological Responses of the Silkworm, Bombyx mori, in Space (Rad Silk) ....................................................................................... 56 Study of the Growth Potential of Statoconia in the Organ of Balance of Gastropods in Weightlessness (Statokonia) ............................................................................................................... 59 Cellular Modifications within the Vestibulo-ocular System during Adaptation to Microgravity in a Developing Amphibian, Xenopus laevis (Xenopus) ............................................................................. 61 Avian Development Facility (ADF), Two Investigations ...................................................................... 63 Embryonic Development of Amphibians in Weightlessness (Aquarius) ............................................. 66 Commercial Biomedical Testing Module: Effects of Osteoprotegerin on Bone Maintenance in microgravity (CBTM) ........................................................................................................................... 68 Commercial Biomedical Testing Module-2: (CBTM-2) ........................................................................ 71 Commercial Biomedical Testing Module-3: Assessment of sclerostin antibody as a novel bone forming agent for prevention of spaceflight-induced skeletal frgility in mice (CBTM-3-Sclerostin Antibody)............................................................................................................................................. 77 Commercial Biomedical Testing Module-3: STS-135 spaceflight’s affects on vascular atrophy in the hind limbs of mice (CBTM-3-Vascular Atrophy) .................................................................................. 79 C. elegans to Assess Genomic Damage on Long-Duration Flights (Elerad) ......................................... 82 Mice Drawer System (MDS) ................................................................................................................ 84 Mouse Antigen Specific CD4+ T Cell Priming and Memory Response during Spaceflight (Mouse Immunology) ....................................................................................................................................... 90 Effect of SpaceFlight on Innate Immunity to Respiratory Viral Infections (Mouse Immunology-2) ... 93 2 Cellular Biology Role of Weightlessness on Metabolism (Actin) .................................................................................. 94 The Antibody V(D)J Recombination Machinery in Normal and Altered Gravity (Amphibody) .......... 95 Effect of Spaceflight Factors on the Expression of Producer Strains of Interleukin 1α, 1β, and Aryl (Aryl-1/Aryl-2), Two Investigations ..................................................................................................... 97 BIOKon In Space (BIOKIS), Seven Investigations ................................................................................. 99 Bone Proteomics (BOP) ..................................................................................................................... 103 Cellular Biotechnology Operations Support Systems (CBOSS), Seven Investigations ...................... 104 Study on the Development of Methods to Produce Artificial Cartilage (Chondro) .......................... 109 Control of Cell Differentiation and Morphogenesis of Amphibian Culture Cells (DomeGene) ........ 110 Investigation of the Osteoclastic and Osteoblastic Responses to Microgravity Using Goldfish Scales (Fish Scales) ....................................................................................................................................... 111 Bone Cell Mechanosensitivity in Weightlessness (FLOW) ................................................................ 114 Fischer Rat Thyroid Low Serum 5 (FRTL5) ......................................................................................... 115 Expression of Microbial Genes in Space (Gene Expression) ............................................................. 116 Influence of Weightlessness on the Activation of NF-ĸβ Proteins (Kappa) ...................................... 118 Role of Interleukin-2 Receptor in Signal Transduction and Gravisensing Threshold of T-Lymphocytes (Leukin-2) .......................................................................................................................................... 119 Detection of changes in LOH Profile of TK mutants of Human Cultured Cells (LOH) ....................... 121 Cell to Cell Interaction of Monocytes and T-Lymphocytes in Microgravity (MIA) ............................ 123 Effects of Microgravity on Expression of Calcium Channels in Myocytes (Myocyte) ....................... 125 Cbl-b-Mediated Protein Ubiquitination Downregulates the Response of Skeletal Muscle Cells to Growth Factors in Space (MyoLab) ................................................................................................... 126 Effects of Microgravity on the Haemopoietic System: A Study on Neocytolysis (Neocytolysis) ...... 129 Biological Effects of Space Radiation and Microgravity on Mammalian Cells (Neuro Rad) ............. 131 Eyespots and Macular Pigments Extracted from Algal Organisms Immobilized in Organic Matrix with the Purpose to Protect Astronaut’s Retina (Night Vision) ................................................................ 133 Natural Killer Cell Activity in Microgravity (NKA) .............................................................................. 135 National Laboratory Pathfinder-Cells (NLP-Cells), Two Investiations ............................................... 137 Pathway Different Activators (PADIAC) ............................................................................................ 140 Mechanisms and Functional Consequences of Protein Kinase C Isoform Translocation in Monocytes Exposed to Microgravity (PKinase) ................................................................................................... 141 Gene Expression of p53-regulated Genes in Mammalian Cultured Cells After Exposure to a Space Environment (Rad Gene) ................................................................................................................... 143 Signalling through Rho GTPases in microgravity (Rho Signalling)..................................................... 146 Role of Apoptosis in Lymphocyte Depression (ROALD) .................................................................... 147 Role of Apoptosis in Lymphocyte Depression-2 (ROALD-2) ............................................................. 148 3 Spaceflight of Huvec: an Integrated eXperiment (SPHINX) .............................................................. 149 StelSys Liver Cell Function Research (StelSys) .................................................................................. 150 Space Tissue Loss (STL), Five Investigations ...................................................................................... 152 Bone Marrow Stroma Cell Differentiation and Mesenchymal Tissue Reconstruction in Microgravity (Stroma-2) ......................................................................................................................................... 155 Effects of Microgravity on the Action of Vitamin D in Osteoblasts (Vitamin D) ............................... 157 Macromolecular Crystal Growth Advanced Protein Crystallization Facility (APCF) , Eight Investigations ............................................ 159 Commercial Protein Crystal Growth-High Density (CPCG-H) ............................................................ 165 Dynamically Controlled Protein Crystal Growth (DCPCG) ................................................................ 169 Granada Crystallisation Facility (GCF) ............................................................................................... 171 Identifying and Investigating Surface Glycoproteins E1-E2 of Alphaviruses on Earth and in Space (Glikoproteid) .................................................................................................................................... 173 Japan Aerospace Exploration Agency – Granada Crystalization Facility High Quality Protein Crystallization Experiment (JAXA-GCF), 238 Investigations .............................................................. 174 Japan Aerospace Exploration Agency Protein Crystal Growth (JAXA PCG), 114 Investigations ....... 177 Protein Crystallization Diagnostics Facility - Influence of mass transport and surface growth processes on protein crystal perfection (PCDF) ................................................................................ 180 Protein Crystal Growth-Enhanced Gaseous Nitrogen Dewar (PCG-EGN), Thirty Investigations ...... 182 Protein Crystal Growth-Single Locker Thermal Enclosure System (PCG-STES), Nine Investigations 185 Protein Crystal Growth Monitoring by Digital Holographic Microscope for the International Space Station (PROMISS-1, -2, -3 and -4), Four Investigations ................................................................... 191 Structural Research on Protein Candidates for an AIDS Vaccine in Earth and Space Conditions (Vaktsina-K) ....................................................................................................................................... 193 Microbiology Development of Methods and Onboard Equipment to Assure Aseptic Conditions Performing Biotechnology Experiments During Manned Spaceflight (Aseptik) .................................................. 194 Cultivating Escheria coli Producer of CAF1 Protein in Weightlessness (Astrovaktsina) ................... 196 Study of the Effects of Spaceflight Factors on Bacteriophages (Bakteriofag/Bakteriofag-L), Two Investigations .................................................................................................................................... 198 Bacterial Acclimation and Adaptation to the Space Environment Conditions-A (BASE-A) ............... 200 Bacterial Acclimation and Adaptation to the Space Environment Conditions-B/-C (BASE-B/-C), Two Investigations .................................................................................................................................... 202 Study of the Effects of Spaceflight Factors on the Technological and Biomedical Characteristics of Bifidobacteria (Bif) ............................................................................................................................ 203 Obtaining Highly-Efficient Strains of Microorganisms for the Production of Biological Petroleum Degrading Compounds, Organophosphate Substances, Plant Protection Means, and 4 Exopolysaccharides used in the Petroleum Industry (Bioekologiya-M/Bioekologiya-R), Two Investigations .................................................................................................................................... 204 Growth and Survival of Colored Fungi in Space-A (CFS-A) ................................................................ 206 International Space Station High Efficiency Particle Filter Analysis (ISS High Efficiency Particle Filter Analysis) ............................................................................................................................................ 208 Microbial Drug Resistance and Virulence (MDRV)............................................................................ 209 Microbiological Experiment on Space Station About Gene Expression-1 and 2 (MESSAGE-1 and MESSAGE-2), Two Investigations ...................................................................................................... 212 Gravitational Effects on Biofilm Formation During Spaceflight (Micro-2) & Microbial biofilm formation during spaceflight (Micro-2A), Two Investigations .......................................................... 214 Genotypic and Phenotypic Changes in Yeast Related to Selective Growth Pressures Unique to Microgravity (Micro-4) ...................................................................................................................... 216 Effect of Spaceflight on Microbial Gene Expression and Virulence (Microbe) ................................. 217 Microbial dynamics in International Space Station (Microbe-I/II), Two Investigations ................... 221 Microbial life in Space: Response to environmental factors in a space vehicle (Microspace) ......... 223 Passive Observatories for Experiments Microbial Systems (POEMS) ............................................... 224 Streptococcus pneumoniae Expression of Genes in Space I and II (SPEGIS I and SPEGIS II), Two Investigations .................................................................................................................................... 226 eValuatIon And monitoring of microBiofiLms insidE International Space Station (VIABLE ISS) ...... 228 Yeast In No Gravity - The Influence of Microgravity on Cellular Adhesions, Biofilm Formation and In Invasive Growth in the Model Eukaryote Saccharomyces cerevisiae (Yeast-B: Part 2) .................... 229 Yeast-Group Activation Packs (Yeast-GAP) ....................................................................................... 231 Yeast In No Gravity - The Influence of Microgravity on Cellular Adhesions, Biofilm Formation and In Invasive Growth in the Model Eukaryote Saccharomyces cerevisiae (YING-A: Part 1) .................... 234 Microencapsulation Microencapsulation Electrostatic Processing Systems (MEPS) ........................................................ 236 Plant Biology Advanced AstrocultureTM (ADVASC) ................................................................................................. 239 Advanced Plant Experiments - Cambium (APEX-Cambium) ............................................................. 242 Advanced Plant Experiment - Canadian Space Agency 2 (APEX-CSA2)............................................. 243 Arabidopsis thaliana in Space: Perception of Gravity, Signal Transductions and Gravitresponse in Higher Plants (AT-Space) ................................................................................................................... 245 Biomass Production System (BPS) .................................................................................................... 246 Biological Research in Canisters-16 (BRIC-16), Three Investigations ................................................ 249 Biological Research in Canisters Symbiotic Nodulation in a Reduced Gravity Environment (BRIC- SyNRGE) ............................................................................................................................................ 253 Reverse Genetic Approach to Exploring Genes Responsible for Cell Wall Dynamics in Supporting Tissues of Arabidopsis Under Microgravity Conditions (Cell Wall) ................................................... 255 5 Regulation by Gravity of Ferulate Formation in Cell Walls of Rice Seedlings (Ferulate) .................. 257 Gravity Related Genes in Arabidopsis - A (Genara-A) ....................................................................... 259 Study into the Interaction of Effects of Light and Gravity on the Growth Process of Plants (GraPhoBox) ...................................................................................................................................... 261 Threshold Acceleration for Gravisensing-1 (Gravi-1) ........................................................................ 262 Gravigen ............................................................................................................................................ 264 Hydrotropism and Auxin-Inducible Gene Expression in Roots Grown under Microgravity Conditions (Hydro Tropi) ..................................................................................................................................... 265 Validating Vegetable Production Unit (VPU) Plante, Protocols, Procedures and Requirements (P3R) Using Currently Existing Flight Resources (Lada-VPU-P3R) .............................................................. 267 Study of Mass-Exchange Properties of Capillary-Porous Bodies, Root Habitable Media, in Spaceflight Conditions (Massoperenos) .............................................................................................................. 270 Molecular and Plant Physiological Analyses of the Microgravity Effects on Multigeneration Studies of Arabidopsis thaliana (Multigen) ................................................................................................... 272 NanoRacks-Terpene Extraction in Microgravity (NanoRacks-Terpene) ........................................... 274 The Optimization of Root Zone Substrates for reduced Gravity Experiments Program (ORZS) ....... 275 Photosynthesis Experiment and System Testing and Operation (PESTO) ........................................ 277 Plant Generic Bioprocessing Apparatus (PGBA) ............................................................................... 280 Plant Signaling (Plant Signaling) ........................................................................................................ 282 Effect of weightlessness on the distribution of calcium in the statocytes of Rapeseed roots, Brassica napus (Polca) ..................................................................................................................................... 284 Role of Microtubule-Membrane-Cell Wall Continuum in Gravity Resistance in Plants (Resist Wall) .......................................................................................................................................................... 285 Effects of the Space Environment on the Nuclear Structure and Function of Plant Root meristematic Cells Grown in Microgravity (Root) ................................................................................................... 287 Life Cycle of Higher Plants under Microgravity Conditions (Space Seed) ......................................... 289 Transgenic Arabisopsis Gene Expression System (TAGES) ................................................................ 290 Analysis of a Novel Sensory Mechanism in Root Phototropism (Tropi) ........................................... 292 Influence of Gravity on the Cytoskeleton and the Determination of the Division Plane in Plants (Tubul/Tubul-2), Two Investigations ................................................................................................. 296 Vine in Near Orbit (VINO) ................................................................................................................. 297 Waving and Coiling of Arabidopsis Roots at Different g-levels (WAICO and WAICO-2), Two Investigations .................................................................................................................................... 298 Vaccine Development Optimizing Heterologous Expression in Saccharomyces Yeast in Microgravity Based on the Example of Hepatitis B Surface Antigen Synthesis (Antigen) .......................................................................... 299 National Laboratory Pathfinder-Vaccine (NLP-Vaccine), Three Investigations ................................ 300 Recombinant Attenuated Salmonella Vaccine (RASV) ...................................................................... 302 6 Earth and Space Science ............................................................................................................. 303 Astrobiology European Technology Exposure Facility - Exposure Experiment - Adapt (EuTEF-Expose-Adapt) .... 304 European Technology Exposure Facility - Exposure Experiment - Life (EuTEF-Expose-Life) ............ 306 European Technology Exposure Facility - Exposure Experiment - Process (EuTEF-Expose-Process) 308 European Technology Exposure Facility - Exposure Experiment - Protect (EuTEF-Expose-Protect) 310 European Technology Exposure Facility - Exposure Experiment – Seeds (EuTEF-Expose-Seeds) .... 312 Expose – R Photochemical Processing of Amino Acids in Earth Orbit (Expose-R-Amino) ................ 314 Expose – R Screening of Ultra-Violet Radiation in Endothilic and Microalgal Communities from Antartica (Expose-R-Endo) ................................................................................................................ 314 Expose – R Evolution of Organic Matter in Space (Expose-R-Organic) ............................................. 316 Expose – R Exposure of Osmophilic Microbes to Space Environment Expose-R-Osmo) .................. 317 Expose – R Photo DNA Photodamage: Measurements of Vacuum Solar Radiation-induced DNA Damages Within Spores (Expose-R-Photo) ....................................................................................... 318 Expose – R Responses of Phage T7, Phage DNA and Polycrystalline Uracil to the Space Environment (Expose-R PUR) .................................................................................................................................. 319 Expose – R Radiation Risks Raiometer-Dosimeter (Expose-R R3DR) ................................................ 320 Expose – R Spores in Artificial Meteorites (Expose-R Spores) .......................................................... 322 Expose – R Mutational Spectra of Bacillus subtilis Spores and Plasmid DNA Exposed to High Vacuum and Solar UV Radiation in the Space Environment (Expose-R Subtil) ............................................... 323 Alpha Magnetic Spectrometer - 02 (AMS-02) ................................................................................... 324 Study of the Fluxes of Fast and Thermal Neutrons (BTN-Neytron), Six Investigations .................... 327 Monitor of All-Sky X-Ray Image (MAXI) ............................................................................................ 330 Searching for Low-Energy Heavy Nuclei of Solar and Galactic Origin using the PLAstic Track ANalyzer (PLATAN), Five Investigations ........................................................................................................... 334 Earth Remote Sensing Agricultural Camera – (AgCam) AgCam name used historically from 2005-2010, later version known as ISSAC ............................................................................................................................................. 337 Coordinated Auroral Photography from Earth and Space (AuroraMax) .......................................... 339 Crew Earth Observations (CEO) ........................................................................................................ 341 Crew Earth Observations - International Polar Year (CEO-IPY)......................................................... 345 Stability of Geographical Position and Configuration of Borders of Bioproductive Water Zones of the World’s Oceans Observed by Orbital Station Crews (Diatomeya) .................................................... 347 HICO and RAIDS Experiment Payload - Hyperspectral Imager for the Coastal Ocean (HREP-HICO) 349 Observation of Environmental Phenomena (Imedias) ..................................................................... 354 International Space Station Agricultural Camera (ISSAC) ................................................................. 355 Lightning and Sprite Observations (LSO-B, LSO-F, LSO-H, LSO-S), Four Investigations .................... 356 7 Investigating Atmospheric Bursts of Gamma-Ray and Optical Emissions During Thunderstorm Activity (Molniya-Gamma) ................................................................................................................ 359 Study of the Electrodynamic Interaction Processes in the Earth’s Atmosphere, Ionosphere, and Magnetosphere Using the ВФС-3М Videophotometric System (Molniya-SM) ................................ 361 Superconducting Submillimeter-Wave Limb-Emission Sounder (SMILES) ....................................... 363 Observation, in the Near-IR Range of the Spectrum, of Wave Disturbances in the Middle Atmosphere (Volny) .......................................................................................................................... 366 Monitoring Seismic Effects—Bursts of High-Energy Particles in Near-Earth Space (Vsplesk) .......... 368 Heliophysics Sun monitoring on the External Payload Facility of Columbus – SOLar Auto-Calibrating EUV/UV Spectrophotometers (Solar-SOLACES) .............................................................................................. 370 Sun monitoring on the External Payload Facility of Columbus –SOLar SPECtral Irradiance Measurements (Solar-SOLSPEC) ....................................................................................................... 372 Sun monitoring on the External Payload Facility of Columbus –SOlar Variable and Irradiance Monitor (Solar- SOVIM) .................................................................................................................... 374 Near-Earth Space Environment Altering the Ionosphere by Pulsed Plasma Sources (Impuls Stage 1), Three Investigations ............ 375 Observation of the Reflective Characteristics of the Spacecraft Plasma Environment during Engine Firing in Space Using Ground-Based Instruments (Plazma-Progress) ............................................... 377 Processes of Relaxation in the Ultraviolet Band Spectrum by High-Velocity Interaction of Exhaust Products on ISS (Relaksatsiya), Five Investigations ........................................................................... 380 Space Environment Data Acquisition Equipment - Attached Payload (SEDA-AP), Seven Investigations .......................................................................................................................................................... 384 Service Module/Micro-Particles Capturer and Space Environment Exposure Device (SM/MPAC and SEED), Two Investigations ................................................................................................................. 386 Educational Activities and Outreach ......................................................................................... 389 Classroom Versions of ISS Experiments Agrospace Experiments Suite (AES), Two Investigations .................................................................. 390 Commercial Generic Bioprocessing Apparatus Science Insert-01: C. elegans and Seed Germination (CSI-01), Two Investigations .............................................................................................................. 392 Commercial Generic Bioprocessing Apparatus Science Insert-02: Silicate Garden, Seed Germination, Plant Cell Culture and Yeast (CSI-02), Four Investigations ................................................................ 394 Commercial Generic Bioprocessing Apparatus Science Insert-03: Spiders and Butterflies (CSI-03), Two Investigations ............................................................................................................................ 396 Commercial Generic Bioprocessing Apparatus Science Insert-05: Spiders, Fruit Flies, and Directional Plant Growth (CSI-05), Three Investigations ..................................................................................... 398 Space Exposed Experiment Developed for Students (Education- SEEDS) ........................................ 400 European space Agency – Education Payload Operations – Foam-S (ESA-EPO-Foam-S) ................. 402 8 Demonstrating the Effect of Restoring the Form of Billets Made of Cellular Polymer Materials (MATI-75) .......................................................................................................................................... 403 Oil Emulsions Experiment (OEE) ....................................................................................................... 405 Seeds in Space (Seeds) ...................................................................................................................... 406 Taste in Space ................................................................................................................................... 408 Cultural Activities European Space Agency – Education Payload Operations – Fuji 3D (ESA-EPO-Fuji 3D) ................... 409 Creating, Preparing, and Launching Small Spacecraft during Extravehicular Activity (RadioSkaf) ... 410 Space Seeds for Asian Future (SSAF) ................................................................................................. 412 Ucyu Renshi: Connecting Global People with Words (Space Poem Chain) ...................................... 414 Educational Competitions Kids in Micro-Gravity (Kids in Micro-G), Two Investigations ............................................................ 415 Synchronized Position Hold, Engage, Reorient, Experimental Satellites-Zero-Robotics (SPHERES- Zero-Robotics) ................................................................................................................................... 418 Educational Demonstrations Amateur Radio on the International Space Station-European Space Agency (ARISS-ESA) .............. 422 DREAMTiME (DREAMTiME) .............................................................................................................. 423 E-Learning ......................................................................................................................................... 424 Educational Payload Operations (EPO) ............................................................................................. 425 Educational Payload Operations-Cloud Observation-Demonstrations (EPO-Cloud Observation- Demos) .............................................................................................................................................. 426 Educational Payload Operations-Demonstrations (EPO-Demos) ..................................................... 427 Educational Payload Operations-Educator (EPO- Educator) ............................................................ 428 Educational Payload Operations-International Toys in Space (EPO-International Toys in Space) ... 429 Education Payload Operations - Kit C: Plant Growth Chambers (EPO-Kit C) .................................... 431 Education Payload Operations - Kit D (EPO-Kit D) ............................................................................ 432 Education Payload Operations–Lewis and Clark-Demonstrations (EPO-Lewis and Clark Demo) ..... 433 Education Payload Operations–Museum Aerospace Education Alliance (EPO-MAEA) .................... 434 Education Payload Operations–Robotics (EPO-Robo) ...................................................................... 435 Education Payload Operations–Sesame Street Demonstration (EPO-Sesame Street-Demos) ........ 436 European Space Agency – Education Payload Operations – Greenhouse (ESA-EPO-Greenhouse) . 438 European Space Agency – Education Payload Operations – Mission-X (ESA-EPO Mission-X) .......... 440 Scientific and Educational Demonstration of Physical Laws and Phenomena in Microgravity (Fizika- Obrazovaniye), Three Investigations ................................................................................................ 441 Popularizing Achievements in Russian Manned Space Exploration (Great Start) ............................ 444 Production of Japan Aerospace Exploration Agency - Astronaut Report (JAXA-AstroReport) ......... 446 9 Japan Aerospace Exploration Agency Education Payload Observation (JAXA EPO), Sixteen Investigations .................................................................................................................................... 447 Kibo Kids Tour ................................................................................................................................... 454 Study of the Dynamics of a System of Charged Particles in a Magnetic Field in Microgravity Conditions (Kulonovskiy Kristall) ....................................................................................................... 455 LEGO® Bricks (LEGO Bricks) ................................................................................................................ 457 Lessons from Space-2 (LES-2)............................................................................................................ 459 Lessons from Space-3 (LES-3)............................................................................................................ 460 NanoRacks-Faith Christian Academy-Concrete Mixing Experiment (NanoRacks-FCA-Concrete Mixing) .............................................................................................................................................. 461 NanoRacks-Fremont Christian High School-Micro-Robot (NanoRacks-FCHS-Robot) ....................... 462 PhotosynthTM Three-Dimensionsal Modeling of ISS Interior and Exterior (Photosynth).................. 463 Sally Ride Earth Knowlegde Acquired by Middle School Students (Sally Ride EarthKAM) .............. 464 using a Network of Ground Receivers (Ten-Mayak) ......................................................................... 466 Education Payload Operations-Tomatosphere II (EPO-Tomatosphere II) ........................................ 468 Tomatosphere-III ............................................................................................................................... 469 Try Zero-Gravity (Try Zero-G) ............................................................................................................ 471 European Space Agency – Mission DVD Series (Video-1, Video-2, Video-3, DVD-4) Four Investigations .................................................................................................................................... 472 Video Lesson European Space Agency - I (VLE-I) ............................................................................. 474 YouTube Space Lab (YouTube Space Lab) ......................................................................................... 475 Engineering Education Spacecraft and Modern Technologies for Personal Communications (MAI-75) .............................. 477 Student-Developed Investigations Analysis of Inertial Solid Properties (APIS) ........................................................................................ 479 Bug Energy- Study of Output of Bacterial Fuel Cells in Weightlessness (BugNRG) .......................... 480 Electrostatic Self-assembly Demonstration (ESD) ............................................................................ 481 NanoRacks-National Center for Earth and Space Science Education (NanoRacks-NCESSE-1 & NanoRacks-NCESSE-2), Two Investigations ....................................................................................... 482 NanoRacks-Valley Christian High School (NanoRacks-VCHS), Four Investigations ........................... 485 NanoRacks-Whittier Christian High School-E. Coli Bacteria and Kanamycin Antibiotic (NanoRacks- WCHS-E. Coli and Kanamycin) ........................................................................................................... 487 Space Experiment Module (SEM) ..................................................................................................... 488 Test of the Basic Principles of Mechanics in Space (Thebas) ............................................................ 489 University Research Centers-Microbial-1 (URC-Microbial-1) ........................................................... 491 Under the Background Influence (UTBI) ........................................................................................... 492 10

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1 Table of Contents Introduction .................................................................................................................................. 24 Executive Summary ...................................................................................................................... 29 Biology and Biotechnology .......................................................................................................... 33 Animal Biology - Invertebrates Drosophila Motility, Behaviour and Ageing (Ageing) .......................................................................... 34 Study of the Resistance of a Modeled Closed Ecosystem and Chains of its Components in Microgravity (Akvarium) ..................................................................................................................... 36 Caenorhabditis elegans RNAi Space Experiment (CERISE) .................................................................. 39 Commercial Generic Bioprocessing Apparatus (CGBA), Three Investigations .................................... 41 Crickets in Space-2 (CRISP-2) .............................................................................................................. 44 Fungal Pathogenesis, Tumorigenesis, and Effects of Hose Immunity in Space (FIT) .......................... 46 International Caenorhabditis Elegans Experiment First Flight (ICE-First), Eight Investigations ......... 48 NanoRacks-University of Florida-1 (NanoRacks-UF-Squids-1) ............................................................ 55 Integrated Assessment of Long-term Cosmic Radiation Through Biological Responses of the Silkworm, Bombyx mori, in Space (Rad Silk) ....................................................................................... 56 Study of the Growth Potential of Statoconia in the Organ of Balance of Gastropods in Weightlessness (Statokonia) ............................................................................................................... 59 Cellular Modifications within the Vestibulo-ocular System during Adaptation to Microgravity in a Developing Amphibian, Xenopus laevis (Xenopus) ............................................................................. 61 Avian Development Facility (ADF), Two Investigations ...................................................................... 63 Embryonic Development of Amphibians in Weightlessness (Aquarius) ............................................. 66 Commercial Biomedical Testing Module: Effects of Osteoprotegerin on Bone Maintenance in microgravity (CBTM) ........................................................................................................................... 68 Commercial Biomedical Testing Module-2: (CBTM-2) ........................................................................ 71 Commercial Biomedical Testing Module-3: Assessment of sclerostin antibody as a novel bone forming agent for prevention of spaceflight-induced skeletal frgility in mice (CBTM-3-Sclerostin Antibody)............................................................................................................................................. 77 Commercial Biomedical Testing Module-3: STS-135 spaceflight’s affects on vascular atrophy in the hind limbs of mice (CBTM-3-Vascular Atrophy) .................................................................................. 79 C. elegans to Assess Genomic Damage on Long-Duration Flights (Elerad) ......................................... 82 Mice Drawer System (MDS) ................................................................................................................ 84 Mouse Antigen Specific CD4+ T Cell Priming and Memory Response during Spaceflight (Mouse Immunology) ....................................................................................................................................... 90 Effect of SpaceFlight on Innate Immunity to Respiratory Viral Infections (Mouse Immunology-2) ... 93 2 Cellular Biology Role of Weightlessness on Metabolism (Actin) .................................................................................. 94 The Antibody V(D)J Recombination Machinery in Normal and Altered Gravity (Amphibody) .......... 95 Effect of Spaceflight Factors on the Expression of Producer Strains of Interleukin 1α, 1β, and Aryl (Aryl-1/Aryl-2), Two Investigations ..................................................................................................... 97 BIOKon In Space (BIOKIS), Seven Investigations ................................................................................. 99 Bone Proteomics (BOP) ..................................................................................................................... 103 Cellular Biotechnology Operations Support Systems (CBOSS), Seven Investigations ...................... 104 Study on the Development of Methods to Produce Artificial Cartilage (Chondro) .......................... 109 Control of Cell Differentiation and Morphogenesis of Amphibian Culture Cells (DomeGene) ........ 110 Investigation of the Osteoclastic and Osteoblastic Responses to Microgravity Using Goldfish Scales (Fish Scales) ....................................................................................................................................... 111 Bone Cell Mechanosensitivity in Weightlessness (FLOW) ................................................................ 114 Fischer Rat Thyroid Low Serum 5 (FRTL5) ......................................................................................... 115 Expression of Microbial Genes in Space (Gene Expression) ............................................................. 116 Influence of Weightlessness on the Activation of NF-ĸβ Proteins (Kappa) ...................................... 118 Role of Interleukin-2 Receptor in Signal Transduction and Gravisensing Threshold of T-Lymphocytes (Leukin-2) .......................................................................................................................................... 119 Detection of changes in LOH Profile of TK mutants of Human Cultured Cells (LOH) ....................... 121 Cell to Cell Interaction of Monocytes and T-Lymphocytes in Microgravity (MIA) ............................ 123 Effects of Microgravity on Expression of Calcium Channels in Myocytes (Myocyte) ....................... 125 Cbl-b-Mediated Protein Ubiquitination Downregulates the Response of Skeletal Muscle Cells to Growth Factors in Space (MyoLab) ................................................................................................... 126 Effects of Microgravity on the Haemopoietic System: A Study on Neocytolysis (Neocytolysis) ...... 129 Biological Effects of Space Radiation and Microgravity on Mammalian Cells (Neuro Rad) ............. 131 Eyespots and Macular Pigments Extracted from Algal Organisms Immobilized in Organic Matrix with the Purpose to Protect Astronaut’s Retina (Night Vision) ................................................................ 133 Natural Killer Cell Activity in Microgravity (NKA) .............................................................................. 135 National Laboratory Pathfinder-Cells (NLP-Cells), Two Investiations ............................................... 137 Pathway Different Activators (PADIAC) ............................................................................................ 140 Mechanisms and Functional Consequences of Protein Kinase C Isoform Translocation in Monocytes Exposed to Microgravity (PKinase) ................................................................................................... 141 Gene Expression of p53-regulated Genes in Mammalian Cultured Cells After Exposure to a Space Environment (Rad Gene) ................................................................................................................... 143 Signalling through Rho GTPases in microgravity (Rho Signalling)..................................................... 146 Role of Apoptosis in Lymphocyte Depression (ROALD) .................................................................... 147 Role of Apoptosis in Lymphocyte Depression-2 (ROALD-2) ............................................................. 148 3 Spaceflight of Huvec: an Integrated eXperiment (SPHINX) .............................................................. 149 StelSys Liver Cell Function Research (StelSys) .................................................................................. 150 Space Tissue Loss (STL), Five Investigations ...................................................................................... 152 Bone Marrow Stroma Cell Differentiation and Mesenchymal Tissue Reconstruction in Microgravity (Stroma-2) ......................................................................................................................................... 155 Effects of Microgravity on the Action of Vitamin D in Osteoblasts (Vitamin D) ............................... 157 Macromolecular Crystal Growth Advanced Protein Crystallization Facility (APCF) , Eight Investigations ............................................ 159 Commercial Protein Crystal Growth-High Density (CPCG-H) ............................................................ 165 Dynamically Controlled Protein Crystal Growth (DCPCG) ................................................................ 169 Granada Crystallisation Facility (GCF) ............................................................................................... 171 Identifying and Investigating Surface Glycoproteins E1-E2 of Alphaviruses on Earth and in Space (Glikoproteid) .................................................................................................................................... 173 Japan Aerospace Exploration Agency – Granada Crystalization Facility High Quality Protein Crystallization Experiment (JAXA-GCF), 238 Investigations .............................................................. 174 Japan Aerospace Exploration Agency Protein Crystal Growth (JAXA PCG), 114 Investigations ....... 177 Protein Crystallization Diagnostics Facility - Influence of mass transport and surface growth processes on protein crystal perfection (PCDF) ................................................................................ 180 Protein Crystal Growth-Enhanced Gaseous Nitrogen Dewar (PCG-EGN), Thirty Investigations ...... 182 Protein Crystal Growth-Single Locker Thermal Enclosure System (PCG-STES), Nine Investigations 185 Protein Crystal Growth Monitoring by Digital Holographic Microscope for the International Space Station (PROMISS-1, -2, -3 and -4), Four Investigations ................................................................... 191 Structural Research on Protein Candidates for an AIDS Vaccine in Earth and Space Conditions (Vaktsina-K) ....................................................................................................................................... 193 Microbiology Development of Methods and Onboard Equipment to Assure Aseptic Conditions Performing Biotechnology Experiments During Manned Spaceflight (Aseptik) .................................................. 194 Cultivating Escheria coli Producer of CAF1 Protein in Weightlessness (Astrovaktsina) ................... 196 Study of the Effects of Spaceflight Factors on Bacteriophages (Bakteriofag/Bakteriofag-L), Two Investigations .................................................................................................................................... 198 Bacterial Acclimation and Adaptation to the Space Environment Conditions-A (BASE-A) ............... 200 Bacterial Acclimation and Adaptation to the Space Environment Conditions-B/-C (BASE-B/-C), Two Investigations .................................................................................................................................... 202 Study of the Effects of Spaceflight Factors on the Technological and Biomedical Characteristics of Bifidobacteria (Bif) ............................................................................................................................ 203 Obtaining Highly-Efficient Strains of Microorganisms for the Production of Biological Petroleum Degrading Compounds, Organophosphate Substances, Plant Protection Means, and 4 Exopolysaccharides used in the Petroleum Industry (Bioekologiya-M/Bioekologiya-R), Two Investigations .................................................................................................................................... 204 Growth and Survival of Colored Fungi in Space-A (CFS-A) ................................................................ 206 International Space Station High Efficiency Particle Filter Analysis (ISS High Efficiency Particle Filter Analysis) ............................................................................................................................................ 208 Microbial Drug Resistance and Virulence (MDRV)............................................................................ 209 Microbiological Experiment on Space Station About Gene Expression-1 and 2 (MESSAGE-1 and MESSAGE-2), Two Investigations ...................................................................................................... 212 Gravitational Effects on Biofilm Formation During Spaceflight (Micro-2) & Microbial biofilm formation during spaceflight (Micro-2A), Two Investigations .......................................................... 214 Genotypic and Phenotypic Changes in Yeast Related to Selective Growth Pressures Unique to Microgravity (Micro-4) ...................................................................................................................... 216 Effect of Spaceflight on Microbial Gene Expression and Virulence (Microbe) ................................. 217 Microbial dynamics in International Space Station (Microbe-I/II), Two Investigations ................... 221 Microbial life in Space: Response to environmental factors in a space vehicle (Microspace) ......... 223 Passive Observatories for Experiments Microbial Systems (POEMS) ............................................... 224 Streptococcus pneumoniae Expression of Genes in Space I and II (SPEGIS I and SPEGIS II), Two Investigations .................................................................................................................................... 226 eValuatIon And monitoring of microBiofiLms insidE International Space Station (VIABLE ISS) ...... 228 Yeast In No Gravity - The Influence of Microgravity on Cellular Adhesions, Biofilm Formation and In Invasive Growth in the Model Eukaryote Saccharomyces cerevisiae (Yeast-B: Part 2) .................... 229 Yeast-Group Activation Packs (Yeast-GAP) ....................................................................................... 231 Yeast In No Gravity - The Influence of Microgravity on Cellular Adhesions, Biofilm Formation and In Invasive Growth in the Model Eukaryote Saccharomyces cerevisiae (YING-A: Part 1) .................... 234 Microencapsulation Microencapsulation Electrostatic Processing Systems (MEPS) ........................................................ 236 Plant Biology Advanced AstrocultureTM (ADVASC) ................................................................................................. 239 Advanced Plant Experiments - Cambium (APEX-Cambium) ............................................................. 242 Advanced Plant Experiment - Canadian Space Agency 2 (APEX-CSA2)............................................. 243 Arabidopsis thaliana in Space: Perception of Gravity, Signal Transductions and Gravitresponse in Higher Plants (AT-Space) ................................................................................................................... 245 Biomass Production System (BPS) .................................................................................................... 246 Biological Research in Canisters-16 (BRIC-16), Three Investigations ................................................ 249 Biological Research in Canisters Symbiotic Nodulation in a Reduced Gravity Environment (BRIC- SyNRGE) ............................................................................................................................................ 253 Reverse Genetic Approach to Exploring Genes Responsible for Cell Wall Dynamics in Supporting Tissues of Arabidopsis Under Microgravity Conditions (Cell Wall) ................................................... 255 5 Regulation by Gravity of Ferulate Formation in Cell Walls of Rice Seedlings (Ferulate) .................. 257 Gravity Related Genes in Arabidopsis - A (Genara-A) ....................................................................... 259 Study into the Interaction of Effects of Light and Gravity on the Growth Process of Plants (GraPhoBox) ...................................................................................................................................... 261 Threshold Acceleration for Gravisensing-1 (Gravi-1) ........................................................................ 262 Gravigen ............................................................................................................................................ 264 Hydrotropism and Auxin-Inducible Gene Expression in Roots Grown under Microgravity Conditions (Hydro Tropi) ..................................................................................................................................... 265 Validating Vegetable Production Unit (VPU) Plante, Protocols, Procedures and Requirements (P3R) Using Currently Existing Flight Resources (Lada-VPU-P3R) .............................................................. 267 Study of Mass-Exchange Properties of Capillary-Porous Bodies, Root Habitable Media, in Spaceflight Conditions (Massoperenos) .............................................................................................................. 270 Molecular and Plant Physiological Analyses of the Microgravity Effects on Multigeneration Studies of Arabidopsis thaliana (Multigen) ................................................................................................... 272 NanoRacks-Terpene Extraction in Microgravity (NanoRacks-Terpene) ........................................... 274 The Optimization of Root Zone Substrates for reduced Gravity Experiments Program (ORZS) ....... 275 Photosynthesis Experiment and System Testing and Operation (PESTO) ........................................ 277 Plant Generic Bioprocessing Apparatus (PGBA) ............................................................................... 280 Plant Signaling (Plant Signaling) ........................................................................................................ 282 Effect of weightlessness on the distribution of calcium in the statocytes of Rapeseed roots, Brassica napus (Polca) ..................................................................................................................................... 284 Role of Microtubule-Membrane-Cell Wall Continuum in Gravity Resistance in Plants (Resist Wall) .......................................................................................................................................................... 285 Effects of the Space Environment on the Nuclear Structure and Function of Plant Root meristematic Cells Grown in Microgravity (Root) ................................................................................................... 287 Life Cycle of Higher Plants under Microgravity Conditions (Space Seed) ......................................... 289 Transgenic Arabisopsis Gene Expression System (TAGES) ................................................................ 290 Analysis of a Novel Sensory Mechanism in Root Phototropism (Tropi) ........................................... 292 Influence of Gravity on the Cytoskeleton and the Determination of the Division Plane in Plants (Tubul/Tubul-2), Two Investigations ................................................................................................. 296 Vine in Near Orbit (VINO) ................................................................................................................. 297 Waving and Coiling of Arabidopsis Roots at Different g-levels (WAICO and WAICO-2), Two Investigations .................................................................................................................................... 298 Vaccine Development Optimizing Heterologous Expression in Saccharomyces Yeast in Microgravity Based on the Example of Hepatitis B Surface Antigen Synthesis (Antigen) .......................................................................... 299 National Laboratory Pathfinder-Vaccine (NLP-Vaccine), Three Investigations ................................ 300 Recombinant Attenuated Salmonella Vaccine (RASV) ...................................................................... 302 6 Earth and Space Science ............................................................................................................. 303 Astrobiology European Technology Exposure Facility - Exposure Experiment - Adapt (EuTEF-Expose-Adapt) .... 304 European Technology Exposure Facility - Exposure Experiment - Life (EuTEF-Expose-Life) ............ 306 European Technology Exposure Facility - Exposure Experiment - Process (EuTEF-Expose-Process) 308 European Technology Exposure Facility - Exposure Experiment - Protect (EuTEF-Expose-Protect) 310 European Technology Exposure Facility - Exposure Experiment – Seeds (EuTEF-Expose-Seeds) .... 312 Expose – R Photochemical Processing of Amino Acids in Earth Orbit (Expose-R-Amino) ................ 314 Expose – R Screening of Ultra-Violet Radiation in Endothilic and Microalgal Communities from Antartica (Expose-R-Endo) ................................................................................................................ 314 Expose – R Evolution of Organic Matter in Space (Expose-R-Organic) ............................................. 316 Expose – R Exposure of Osmophilic Microbes to Space Environment Expose-R-Osmo) .................. 317 Expose – R Photo DNA Photodamage: Measurements of Vacuum Solar Radiation-induced DNA Damages Within Spores (Expose-R-Photo) ....................................................................................... 318 Expose – R Responses of Phage T7, Phage DNA and Polycrystalline Uracil to the Space Environment (Expose-R PUR) .................................................................................................................................. 319 Expose – R Radiation Risks Raiometer-Dosimeter (Expose-R R3DR) ................................................ 320 Expose – R Spores in Artificial Meteorites (Expose-R Spores) .......................................................... 322 Expose – R Mutational Spectra of Bacillus subtilis Spores and Plasmid DNA Exposed to High Vacuum and Solar UV Radiation in the Space Environment (Expose-R Subtil) ............................................... 323 Alpha Magnetic Spectrometer - 02 (AMS-02) ................................................................................... 324 Study of the Fluxes of Fast and Thermal Neutrons (BTN-Neytron), Six Investigations .................... 327 Monitor of All-Sky X-Ray Image (MAXI) ............................................................................................ 330 Searching for Low-Energy Heavy Nuclei of Solar and Galactic Origin using the PLAstic Track ANalyzer (PLATAN), Five Investigations ........................................................................................................... 334 Earth Remote Sensing Agricultural Camera – (AgCam) AgCam name used historically from 2005-2010, later version known as ISSAC ............................................................................................................................................. 337 Coordinated Auroral Photography from Earth and Space (AuroraMax) .......................................... 339 Crew Earth Observations (CEO) ........................................................................................................ 341 Crew Earth Observations - International Polar Year (CEO-IPY)......................................................... 345 Stability of Geographical Position and Configuration of Borders of Bioproductive Water Zones of the World’s Oceans Observed by Orbital Station Crews (Diatomeya) .................................................... 347 HICO and RAIDS Experiment Payload - Hyperspectral Imager for the Coastal Ocean (HREP-HICO) 349 Observation of Environmental Phenomena (Imedias) ..................................................................... 354 International Space Station Agricultural Camera (ISSAC) ................................................................. 355 Lightning and Sprite Observations (LSO-B, LSO-F, LSO-H, LSO-S), Four Investigations .................... 356 7 Investigating Atmospheric Bursts of Gamma-Ray and Optical Emissions During Thunderstorm Activity (Molniya-Gamma) ................................................................................................................ 359 Study of the Electrodynamic Interaction Processes in the Earth’s Atmosphere, Ionosphere, and Magnetosphere Using the ВФС-3М Videophotometric System (Molniya-SM) ................................ 361 Superconducting Submillimeter-Wave Limb-Emission Sounder (SMILES) ....................................... 363 Observation, in the Near-IR Range of the Spectrum, of Wave Disturbances in the Middle Atmosphere (Volny) .......................................................................................................................... 366 Monitoring Seismic Effects—Bursts of High-Energy Particles in Near-Earth Space (Vsplesk) .......... 368 Heliophysics Sun monitoring on the External Payload Facility of Columbus – SOLar Auto-Calibrating EUV/UV Spectrophotometers (Solar-SOLACES) .............................................................................................. 370 Sun monitoring on the External Payload Facility of Columbus –SOLar SPECtral Irradiance Measurements (Solar-SOLSPEC) ....................................................................................................... 372 Sun monitoring on the External Payload Facility of Columbus –SOlar Variable and Irradiance Monitor (Solar- SOVIM) .................................................................................................................... 374 Near-Earth Space Environment Altering the Ionosphere by Pulsed Plasma Sources (Impuls Stage 1), Three Investigations ............ 375 Observation of the Reflective Characteristics of the Spacecraft Plasma Environment during Engine Firing in Space Using Ground-Based Instruments (Plazma-Progress) ............................................... 377 Processes of Relaxation in the Ultraviolet Band Spectrum by High-Velocity Interaction of Exhaust Products on ISS (Relaksatsiya), Five Investigations ........................................................................... 380 Space Environment Data Acquisition Equipment - Attached Payload (SEDA-AP), Seven Investigations .......................................................................................................................................................... 384 Service Module/Micro-Particles Capturer and Space Environment Exposure Device (SM/MPAC and SEED), Two Investigations ................................................................................................................. 386 Educational Activities and Outreach ......................................................................................... 389 Classroom Versions of ISS Experiments Agrospace Experiments Suite (AES), Two Investigations .................................................................. 390 Commercial Generic Bioprocessing Apparatus Science Insert-01: C. elegans and Seed Germination (CSI-01), Two Investigations .............................................................................................................. 392 Commercial Generic Bioprocessing Apparatus Science Insert-02: Silicate Garden, Seed Germination, Plant Cell Culture and Yeast (CSI-02), Four Investigations ................................................................ 394 Commercial Generic Bioprocessing Apparatus Science Insert-03: Spiders and Butterflies (CSI-03), Two Investigations ............................................................................................................................ 396 Commercial Generic Bioprocessing Apparatus Science Insert-05: Spiders, Fruit Flies, and Directional Plant Growth (CSI-05), Three Investigations ..................................................................................... 398 Space Exposed Experiment Developed for Students (Education- SEEDS) ........................................ 400 European space Agency – Education Payload Operations – Foam-S (ESA-EPO-Foam-S) ................. 402 8 Demonstrating the Effect of Restoring the Form of Billets Made of Cellular Polymer Materials (MATI-75) .......................................................................................................................................... 403 Oil Emulsions Experiment (OEE) ....................................................................................................... 405 Seeds in Space (Seeds) ...................................................................................................................... 406 Taste in Space ................................................................................................................................... 408 Cultural Activities European Space Agency – Education Payload Operations – Fuji 3D (ESA-EPO-Fuji 3D) ................... 409 Creating, Preparing, and Launching Small Spacecraft during Extravehicular Activity (RadioSkaf) ... 410 Space Seeds for Asian Future (SSAF) ................................................................................................. 412 Ucyu Renshi: Connecting Global People with Words (Space Poem Chain) ...................................... 414 Educational Competitions Kids in Micro-Gravity (Kids in Micro-G), Two Investigations ............................................................ 415 Synchronized Position Hold, Engage, Reorient, Experimental Satellites-Zero-Robotics (SPHERES- Zero-Robotics) ................................................................................................................................... 418 Educational Demonstrations Amateur Radio on the International Space Station-European Space Agency (ARISS-ESA) .............. 422 DREAMTiME (DREAMTiME) .............................................................................................................. 423 E-Learning ......................................................................................................................................... 424 Educational Payload Operations (EPO) ............................................................................................. 425 Educational Payload Operations-Cloud Observation-Demonstrations (EPO-Cloud Observation- Demos) .............................................................................................................................................. 426 Educational Payload Operations-Demonstrations (EPO-Demos) ..................................................... 427 Educational Payload Operations-Educator (EPO- Educator) ............................................................ 428 Educational Payload Operations-International Toys in Space (EPO-International Toys in Space) ... 429 Education Payload Operations - Kit C: Plant Growth Chambers (EPO-Kit C) .................................... 431 Education Payload Operations - Kit D (EPO-Kit D) ............................................................................ 432 Education Payload Operations–Lewis and Clark-Demonstrations (EPO-Lewis and Clark Demo) ..... 433 Education Payload Operations–Museum Aerospace Education Alliance (EPO-MAEA) .................... 434 Education Payload Operations–Robotics (EPO-Robo) ...................................................................... 435 Education Payload Operations–Sesame Street Demonstration (EPO-Sesame Street-Demos) ........ 436 European Space Agency – Education Payload Operations – Greenhouse (ESA-EPO-Greenhouse) . 438 European Space Agency – Education Payload Operations – Mission-X (ESA-EPO Mission-X) .......... 440 Scientific and Educational Demonstration of Physical Laws and Phenomena in Microgravity (Fizika- Obrazovaniye), Three Investigations ................................................................................................ 441 Popularizing Achievements in Russian Manned Space Exploration (Great Start) ............................ 444 Production of Japan Aerospace Exploration Agency - Astronaut Report (JAXA-AstroReport) ......... 446 9 Japan Aerospace Exploration Agency Education Payload Observation (JAXA EPO), Sixteen Investigations .................................................................................................................................... 447 Kibo Kids Tour ................................................................................................................................... 454 Study of the Dynamics of a System of Charged Particles in a Magnetic Field in Microgravity Conditions (Kulonovskiy Kristall) ....................................................................................................... 455 LEGO® Bricks (LEGO Bricks) ................................................................................................................ 457 Lessons from Space-2 (LES-2)............................................................................................................ 459 Lessons from Space-3 (LES-3)............................................................................................................ 460 NanoRacks-Faith Christian Academy-Concrete Mixing Experiment (NanoRacks-FCA-Concrete Mixing) .............................................................................................................................................. 461 NanoRacks-Fremont Christian High School-Micro-Robot (NanoRacks-FCHS-Robot) ....................... 462 PhotosynthTM Three-Dimensionsal Modeling of ISS Interior and Exterior (Photosynth).................. 463 Sally Ride Earth Knowlegde Acquired by Middle School Students (Sally Ride EarthKAM) .............. 464 using a Network of Ground Receivers (Ten-Mayak) ......................................................................... 466 Education Payload Operations-Tomatosphere II (EPO-Tomatosphere II) ........................................ 468 Tomatosphere-III ............................................................................................................................... 469 Try Zero-Gravity (Try Zero-G) ............................................................................................................ 471 European Space Agency – Mission DVD Series (Video-1, Video-2, Video-3, DVD-4) Four Investigations .................................................................................................................................... 472 Video Lesson European Space Agency - I (VLE-I) ............................................................................. 474 YouTube Space Lab (YouTube Space Lab) ......................................................................................... 475 Engineering Education Spacecraft and Modern Technologies for Personal Communications (MAI-75) .............................. 477 Student-Developed Investigations Analysis of Inertial Solid Properties (APIS) ........................................................................................ 479 Bug Energy- Study of Output of Bacterial Fuel Cells in Weightlessness (BugNRG) .......................... 480 Electrostatic Self-assembly Demonstration (ESD) ............................................................................ 481 NanoRacks-National Center for Earth and Space Science Education (NanoRacks-NCESSE-1 & NanoRacks-NCESSE-2), Two Investigations ....................................................................................... 482 NanoRacks-Valley Christian High School (NanoRacks-VCHS), Four Investigations ........................... 485 NanoRacks-Whittier Christian High School-E. Coli Bacteria and Kanamycin Antibiotic (NanoRacks- WCHS-E. Coli and Kanamycin) ........................................................................................................... 487 Space Experiment Module (SEM) ..................................................................................................... 488 Test of the Basic Principles of Mechanics in Space (Thebas) ............................................................ 489 University Research Centers-Microbial-1 (URC-Microbial-1) ........................................................... 491 Under the Background Influence (UTBI) ........................................................................................... 492 10

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