Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics

698 Pages·2000·21.16 MB·English

by  Jeremy Thorner, Scott D. Emr and John N. Abelson (Eds.)

Contributors to Volume 328
Article numbers era in parentheses gniwollof the names of .srotubirtnoc
snoitailiffA listed are .tnerruc
TOM ALBER (17), Department of Molecular RICHARD R. BURGESS (11), McArdle Labora-
and Cell Biology, University of California, tory for Cancer Research, University of Wis-
Berkeley, California 6023-02749 consin, Madison, Wisconsin 53706
KATJA M. ARNDT (17, 22), Department of Mo- GERARD CAGNEY (1), Banting and Best De-
lecular and Cell Biology, University of -ilaC partment of Medical Research, University
fornia, Berkeley, California 6023-02749 of Toronto, Ontario M5G 1L6, Canada
FRANCES H. ARNOLD (26, 27), Division of .X-SIO3qNARF CAMPBELL-VALOIS (14), D~par-
Chemistry and Chemical Engineering, Cali- tement de Biochimie, Universit~ de Mon-
fornia Institute of Technology, Pasadena, treal, Montreal, QuObec H3C 3J7, Canada
California 52119 LEWIS C. CANTLEY (12), Division of Signal
AMI ARONHE1M (4-), Department of Molecular Transduction, Harvard Institutes of Medi-
Genetics and Rappaport Family Institute for cine, Department of Medicine, Beth Israel
Research in Medical Sciences, Technion Is- Deaconess Medical Center, and Department
rael Institute of Technology, Haifa ,69013 of Cell Biology, Harvard Medical School,
Israel Boston, Massachusetts 02215
TERRANCE M. ARTHUR (11), USDA-ARS- JANNETTE CAREY (30), Chemistry Depart-
ment, Princeton University, Princeton, New
RLHUSMARC, Clay Center, Nebraska
Jersey 9001-44580
33986
CAROL A. CHAm~TON (15), Department of
MARK BERGSEID (31), Structural Genomix,
Molecular Pharmacology, Stanford Univer-
San Diego, California 12129
sity School of Medicine, Stanford, Califor-
BRUCE T. BLAKELV (15), Department of Mo- nia 50349
lecular Pharmacology, Stanford University
PAULO S. R. COELHO (33), Department of Mo-
School of Medicine, Stanford, California
lecular, Cellular, and Developmental Biol-
50349
ogy, Yale University, New Haven, Connecti-
ULRICH K. BLASCHKE (29), Laboratory of cut 3018-02560
Synthetic Protein Chemistry, Rockefeller
BRYAN R. CULLEN (20), Howard Hughes
University, New York, New York 12001
Medical Institute and Department of Genet-
HELEN M. BLAU (15), Department of Molecu- ics, Duke University Medical Center, Dur-
lar Pharmacology, Stanford University ham, North Carolina 27710
School of Medicine, Stanford, California
BRIAN C. CUNNINGHAM (21), Sunesis Pharma-
50349
ceuticals, Inc., Redwood City, California
ERIC T. BODER (25), Department of Chemical 36049
Engineering, University of Pennsylvania,
ANTHONY J. DEMAGGIO (10), SOCI Corpora-
Philadelphia, Pennsylvania 19104
tion, Bothell, Washington 12089
MICHAEL A. BRASCH (34), Life Technologies, SHELLEY ANN SED ETAGES (33), Department
Inc., Rockville, Maryland 20850
of Molecular, Cellular, and Developmental
ROGER BRENT (13), The Molecular Sciences Biology, Yale University, New Haven, Con-
Institute, Berkeley, California 40749 necticut 3018-02560
ix
X CONTRIBUTORS TO VOLUME 328
NEHPETS J. EGDELLE (32), Department of Bio- MERE F. ARTSKEOH (10), Qbiogene, Inc.,
chemistry and Molecular Biology, Howard Carlsbad, California 80029
Hughes Medical Institute, Baylor College of SEMAJ C. Hu (18), Department of Biochemis-
Medicine, Houston, Texas 03077 try and Biophysics, Texas A&M University,
IKEDIH HODNE (6), Laboratory of Molecular College Station, Texas 8212-34877
Oncology, MGH Cancer Center, Charles- LuTz SUTUMREJ (24), Department of Bio-
town, Massachusetts 92120 chemistry, University of Zurich, Zurich CH-
HARAS J. ANEHSAF (2), Division of Basic Sci- ,7508 Switzerland
ence, Fox Chase Cancer Center, Philadel- ENIBAS JUNG (22), Department of Biochemis-
phia, Pennsylvania 11191 try, University of Zurich, Zurich CH-
YELNATS SDLEIF (1, 19), Departments of Ge- ,7508 Switzerland
netics and Medicine and Howard Hughes LEZUOG AVOMIRAK (5), Unit~ de Biochimie
Medical Institute, University of Washington, Cellulaire, CNRS URA 2185, lnstitut
Seattle, Washington 0637-59189 Pasteur, Paris, Cedex ,51 France
LLESSUR E. ,YELNIF JR. (3) Center for Molecu- LEAHCIM N1RAK (4), Department of Phar-
lar Medicine and Genetics and Karmanos macology, Laboratory of Gene Regulation
Cancer Institute, Wayne State University and Signal Transduction, University of
School of Medicine, Detroit, Michigan California San Diego, La Jolla, California
10284 6360-39029
C. DLANOR GEYER (13), The Molecular Sci- AMERAJ P. NAHCOK (9), Department of Meta-
ences Institute, Berkeley, California 40749 bolic Diseases, Hoffmann-La Roche, Inc.,
Nutley, New Jersey 07110
SILLYHP NAMDLOG (10), SOCI Corporation,
Bothell, Washington 12089 LIAHKIM G. NINOLOK (3), Center for Molecu-
lar Medicine and Genetics, Wayne State
ERICA A. SIMELOG (2), Division of Basic Sci-
University School of Medicine, Detroit,
ence, Fox Chase Cancer Center, Philadel-
Michigan 10284
phia, Pennsylvania 11191
BRIAN REMEARK (19), Department of Bio-
DRAHCIR H. NAMDOOG (10), The Vollum In-
chemistry, University of Wisconsin, Madi-
stitute, Oregon Health Sciences University,
son, Wisconsin 53706
Portland, Oregon 10279
SUALC REBBERK (22), Maxygen, Inc., Red-
ERIC C. HTIFFIRG (7), Center for Cancer Re-
wood City, California 36049
search, Departments of Biology and Chem-
istry, Massachusetts Institute of Technology, ANUJ RAMUK (33), Department of Molecular,
Cellular, and Developmental Biology, Yale
Cambridge, Massachusetts 02139
University, New Haven, Connecticut
FEZOJ SENAH (24), Department of Biochemis-
3018-02560
try, University of Zurich, Zurich CH-
LEINAD TNADAL (5), Unit~ de Biochimie Cel-
,7508 Switzerland
lulaire, CNRS URA 2185, Institut Pasteur,
EARS O. NOSSNAH (23, 28), Department of
Paris, Cedex ,51 France
Biochemistry, Uppsala University, Uppsala
EIMAM Z. LI (32), Department of Biochemis-
SE-75123, Sweden
try and Molecular Biology, Howard Hughes
SEMAJ L. YELTRAH (34), Life Technologies, Medical Institute, Baylor College of Medi-
Inc., Rockville, Maryland 20850
cine, Houston, Texas 03077
NHOJ A. NAMYEH (31), Invitrogen Corpora- DRAWDE J. LICITRA (7), Center for Cancer
tion, Carlsbad, California 80029 Research, Departments of Biology and
SEMAJ P. RELFFEOH (31), Crosswinds Con- Chemistry, Massachusetts Institute of Tech-
sulting, Anchorage, Alaska 61599 nology, Cambridge, Massachusetts 93120
CONTRIBUTORS TO VOLUME 328 xJ
Dou LIu (32), Department of Biochemistry SAERDNA PLOCK~UN (22, 24), Department of
and Molecular Biology, Howard Hughes Biochemistry, University of Zurich, Zurich
Medical Institute, Baylor College of Medi- ,7508-HC Switzerland
cine, Houston, Texas 03077
DLANOR T. SENIAR (16), Departments of Bio-
NUJ O. Ltu (7), Center for Cancer Research, chemistry and Chemistry, University of Wis-
Departments of Biology and Chemistry, consin, Madison, Wisconsin 53706
Massachusetts Institute of Technology,
DIRGNI REMV (14), Ddpartement de Biochi-
Cambridge, Massachusetts 02139
mie, Universit( de Montreal, Montreal
AUHGNIQ LIU (32), Department of Biochemis-
Quebec H3C 3J7, Canada
try and Molecular Biology, Howard Hughes
Medical Institute, Baylor College of Medi- REEINNEJ D. RmKER (18), Department of Bi-
cine, Houston, Texas 03077 ology, Massachusetts Institute of Technol-
ogy, Cambridge, Massachusetts 02139
EUQINOM A. NOSROL (34), Laboratory of Mo-
lecular Oncology, MGH Cancer Center, G. NEELR1HS ROEDER (33), Department of
Charlestown, Massachusetts 92120 Molecular, Cellular, and Developmental
YRNEH B. NAMWOL (21), Department of Pro- Biology and Department of Genetics, How-
tein Engineering, Genentech, Inc., South ard Hughes Medical Institute, Yale Uni-
San Francisco, California 94080 versity, New Haven, Connecticut -02560
5OO8
TGNEB KIVRENNAM (23, 28), Department of
Biochemistry, Uppsala University, Uppsala OIBAF M. V. RossI (15), Department of Mo-
SE-75123, Sweden lecular Pharmacology, Stanford University
NEHPETS W. KCINHCIM (14), D~partement de School of Medicine, Stanford, California
Biochimie, Universitd de Montreal, Mon- 50349
treal, Quebec H3C 3J7, Canada ABURHD ATPUGNES (19), Departments of Ge-
MOT W. Mum (29), Laboratory of Synthetic netics and Medical Genetics, University of
Protein Chemistry, Rockefeller University, Washington, Seattle, Washington 98195-
New York, New York 12001 0637
NAITSIRK M. RELL3tM (17), Department of AYLI G. IIKSIIRBERES (2), Division of Basic
Molecular and Cell Biology, University of Science, Fox Chase Cancer Center, Phila-
California at Berkeley, Berkeley, California delphia, Pennsylvania 11191
6023-02749
NIXIHZ SHAO (27), Roche Diagnostics, Werk
MARC .S FFOSAN (31), Genomics Institute of Penzberg, 82372 Penzberg, Germany
the Novartis Research Foundation, San
HSItJ-MING SHtH (10), The Vollum Institute,
Diego, California 5211-12129
Oregon Health Sciences University, Port-
KRAM A. ENROBSO (9), Department of Hu-
land, Oregon 10279
man Genetics, Genome Therapeutics Cor-
poration, Waltham, Massachusetts 02154 VEDHCAS .S SIDHU (21), Department of Pro-
tein Engineering, Genentech, Inc., South
NuYH-GNAS PARK (16), Department of Cellu-
San Francisco, California 08049
lar and Molecular Pharmacology, Univer-
sity of California San Francisco, San Fran- NAHTANOJ NIETSREBLIS (29), Laboratory of
cisco, California 0540-34149 Synthetic Protein Chemistry, Rockefeller
ELLEOJ N. REITELLEP (14), D~partement de University, New York, New York 12001
Chimie, Universit~ de Montreal, Montreal, MAILLIW C. SENRAKS (35), Division of Genet-
Qudbec H3C 3J7, Canada ics and Development, Department of Molec-
YELDARB C. ZTE~P (11), McArdle Laboratory ular and Cell Biology, University of Califbr-
for Cancer Research, University of Wiscon- nia at Berkeley, Berkeley, California
sin, Madison, Wisconsin 53706 0023-02749
xii CONTRIBUTORS TO VOLUME 328
LEAHCIM REDYNS (33), Departments of Mo- AHTREBLA J. M. TUOHLAW (6, 34), Dana-
lecular, Cellular, and Developmental Biol- Farber Cancer Institute and Department of
ogy and Molecular Biophysics and BiD- Genetics, Harvard Medical School, Boston,
chemistry, Yale University, New Haven, M sttesuhcas'sa 51120
Connecticut 3018-02560 SEMAJ A. SLLEW (21), Sunesis Pharmaceuti-
YRAG F. ELPMET (34), Life Technologies, Inc., cals, Inc., Redwood City, California 36049
Rockville, Maryland 20850
NIVRAM SNEKCIW (19), Department of Bio-
LISA DATSNORT (23), Department of BiD- chemistry, University of Wisconsin, Madi-
chemistry, Uppsala University, Uppsala SE- son, Wisconsin 53706
,32157 Sweden
LEAKIM NETSREDIW (23), Department of -DiB
RETEP UETZ (1), Departments of Genetics and
chemistry, Uppsala University, Uppsala SE-
Medicine, University of Washington, Seat-
,32157 Sweden
tle, Washington 0637-59189
SENGA NNAMLLU (5), Unit~ de Biochimie Cel- K. DANE Wrlq'RUP (25), Department of
Chemical Engineering and Division of
lulaire, CNRS URA 2185, Institut Pasteur,
Bioengineering, Massachusetts Institute of
Paris, Cedex ,51 France
Technology, Cambridge, Massachusetts
SIXELA ELSIL~IB-E~ILLAV (14), D~partement
93120
de Biochimie, UniversiM de Montreal, Mon-
treal, Quebec H3C 3J7, Canada LEAHCIM B. YAFrE (12), Division of Signal
Transduction, Harvard Institutes of Medi-
REDNAS NAV NED LEVUEH (34), Laboratory of
cine, Departments of Medicine and Surgery,
Molecular Oncology, MGH Cancer Center,
Beth Israel Deaconess Medical Center, Bos-
Charlestown, Massachusetts 02129
ton, Massachusetts 02215
CRAM VIDAL (6, 34), Dana-Farber Cancer
Institute and Department of Genetics, Har- NILIEB GNAHZ (19), Department of Biochem-
vard Medical School, Boston, Massachu- istry, University of Wisconsin, Madison,
setts 51120 Wisconsin 53706
REDNAXELA A. VOKLOV (26, 27), Genecor JIE ZHANG (8), Guilford Pharmaceuticals,
International, Palo Alto, California Inc., Baltimore, Maryland 21224
3101-40349 !UHNIJ GNOHZ (3), Center for Molecular Med-
HPOTSIRHC SREPLOV (9), Center for Molecu- icine and Genetics, Wayne State University
lar Medicine (ZMMK), University of Co- School of Medicine, Detroit, Michigan
logne, Cologne 50931, Germany 10284
Preface
The modern biologist takes almost for granted the rich repertoire of
tools currently available for manipulating virtually any gene or protein of
interest. Paramount among these operations is the construction of fusions.
The tactic of generating gene fusions to facilitate analysis of gene expression
has its origins in the work of Jacob and Monod more than 35 years ago.
The fact that gene fusions can create functional chimeric proteins was
demonstrated shortly thereafter. Since that time, the number of tricks for
splicing or inserting into a gene product various markers, tags, antigenic
epitopes, structural probes, and other elements has increased explosively.
Hence, when we undertook assembling a volume on the applications of
chimeric genes and hybrid proteins in modern biological research, we con-
sidered the job a daunting task.
To assist us with producing a coherent work, we first enlisted the aid
of an Advisory Committee, consisting of Joe Falke, Stan Fields, Brian Seed,
Tom Silhavy, and Roger Tsien. We benefited enormously from their ideas,
suggestions, and breadth of knowledge. We are grateful to them all for
their willingness to participate at the planning stage and for contributing
excellent and highly pertinent articles.
A large measure of the success of this project is due to the enthusiastic
responses we received from nearly all of the prospective authors we ap-
proached. Many contributors made additional suggestions, and quite a
number contributed more than one article. Hence, it became clear early
on that given the huge number of applications of gene fusion and hybrid
protein technology--for studies of the regulation of gene expression, for
lineage tracing, for protein purification and detection, for analysis of protein
localization and dynamic movement, and a plethora of other uses--it would
not be possible for us to cover this subject comprehensively in a single
volume, but in the resulting three volumes, 326, 327, and 328.
Volume 326 is devoted to methods useful for monitoring gene expres-
sion, for facilitating protein purification, and for generating novel antigens
and antibodies. Also in this volume is an introductory article describing
the genesis of the concept of gene fusions and the early foundations of this
whole approach. We would like to express our special appreciation to
Jon Beckwith for preparing this historical overview. Jon's description is
particularly illuminating because he was among the first to exploit gene
and protein fusions. Moreover, over the years, he and his colleagues have
xiii
xiv ECAFERP
continued to develop the methodology that has propelled the use of fusion-
based techniques from bacteria to eukaryotic organisms. Volume 327 is
focused on procedures for tagging proteins for immunodetection, for using
chimeric proteins for cytological purposes, especially the analysis of mem-
brane proteins and intracellular protein trafficking, and for monitoring and
manipulating various aspects of cell signaling and cell physiology. Included
in this volume is a rather extensive section on the green fluorescent protein
(GFP) that deals with applications not covered in Volume 302. Volume
328 describes protocols for using hybrid genes and proteins to identify
and analyze protein-protein and protein-nucleic interactions, for mapping
molecular recognition domains, for directed molecular evolution, and for
functional genomics.
We want to take this opportunity to thank again all the authors who
generously contributed and whose conscientious efforts to maintain the high
standards of the Methods ni Enzymology series will make these volumes of
practical use to a broad spectrum of investigators for many years to come.
We have to admit, however, that, despite our best efforts, we could not
include each and every method that involves the use of a gene fusion or a
hybrid protein. In part, our task was a bit like trying to bottle smoke because
brilliant new methods that exploit the fundamental strategy of using a
chimeric gene or protein are being devised and published daily. We hope,
however, that we have been able to capture many of the most salient and
generally applicable procedures. Nonetheless, we take full responsibility
for any oversights or omissions, and apologize to any researcher whose
method was overlooked.
Finally, we would especially like to acknowledge the expert assistance
of Joyce Kato at Caltech, whose administrative skills were essential in
organizing these books.
YMEREJ RENROHT
Scoxr D. RME
NHOJ N. NOSLEBA
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
detidE yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
detidE yb ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
detidE yb WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
detidE yb RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
detidE yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
detidE yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
detidE by J. M. N1ETSNEWOL
EMULOV XIV. Lipids
detidE yb J. M, NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
detidE yb DNOMYAR B, NOTYALC
EMULOV XVI. Fast Reactions
detidE yb HTENNEK NITSUK
XV
xvi SDOHTEM NI YGOLOMYZNE
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. ¢-BoKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by NHOJ M. NIETSNEWOL
METHODS IN ENZYMOLOGY xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by TREB W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. NAMDRAH DNA BERT W. YELLAM'O
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. NAMDRAH DNA BERT W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by .C H. W. HIRS DNA EGRES N. FEEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. EEFFOH DNA YRAM NELLE SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
lUVX °°o SDOHTEM NI YGOLOMYZNE
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by YEND~S REHCSIELF DNA RETSEL REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. DELucA
EMULOV LVIIL Cell Culture
Edited by MAILLIW B. YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVlE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by LEINAD L. HCIRUP
EMULOV .46 Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and Com-
plex Enzyme Systems)
Edited by LEINAD L. HCaRUP
EMULOV .56 Nucleic Acids (Part I)
Edited by ECNERWAL NAMSSORG DNA K/VIE EVADLOM
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DLANOD B. KCIMROCcM DNA LEUMEL D. THGIRW
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV .86 Recombinant DNA
Edited by YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part C)
Edited by YNOHTNA NAS ORTEIP
EMULOV .07 Immunochemical Techniques (Part A)
Edited by NELEH NAV SIKANUV DNA NHOJ J. ENOGNAL
EMULOV .17 Lipids (Part C)
Edited by NHOJ M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by NHOJ M. NIETSNEWOL