Table Of Content

Contents ISSUE Nos ISSUE No. 1, 15 JANUARY 1999 A Positive Selection for Plasmid Loss in Saccharomyces cerevisiae Using Galactose-Inducible Growth Inhibitory Sequences M. Kawahata, S. Amari, Y. Nishizawa and R. Akada Cloning, Sequence Analysis and Overexpression of a Saccharomyces cerevisiae Endopolygalacturonase- encoding Gene (PGL7) S. Gognies, A. Gainvors, M. Aigle and A. Belarbi Saccharomyces cerevisiae |RR1 Protein is Indirectly Involved in Colony Formation A. Kurlandzka, J. Rytka, B. Rézalska and M. Wysocka identification and Characterization of The Genes for Two Topoisomerase I-Interacting Proteins from Saccharomyces cerevisiae H. Park and R. Sternglanz identification and Characterization of Lysine**° of Yeast Deoxyhypusine Synthase as the Site of Enzyme Intermediate Formation E. C. Wolff and M. H. Park YEAST FUNCTIONAL ANALYSIS REPORTS Disruption and Basic Functional Analysis of Five Chromosome X Novel ORFs of Saccharomyces cerevisiae Reveals YJL125c as an Fssential Gene for Vegetative Growth Jaquet and J.-( Jauniaux Disruption and Basic Phenotypic Analysis of Six Novel Genes from the Left Arm of Chromosome XIV of a sene-siggr mee cerevisiae E. Duenas, J. Revuelta, F. del Rey andC . R. Vazquez de Disruption of Six Novel Genes from the Left Arm of Chromosome XV of Saccharomyces cerevisiae and Basic Phenotypic pepe of the Generated Mutants K. Séron. M.-O. Bk el and R. Haquena r-Tsapis BOOK REVIEW Operating Instructions for the Yeast Machine CALENDAR CURRENT AWARENESS ON YEAST EF No. 2 3 ANUARY 1999 Cloning and Characterization of 1,3-$-Glucanase-Encoding Genes from Non-Conventional Yeasts Esteban, C. R. Vazquez de Aldana and F. del Re Asymmetric Distribution of Phosatidylethanolamine in C. a/bicans: Possible Mediation by CDAT, a Multidrug Transporter 4“ to ATP Binding Cassette (ABC) Superfamily D 1, S. Krishnamurthy [ B. lL xit, C. M. Gupta, D. Sanglard and R. Prasad Secretion of a Protoxin Post entititiiantie Controlled by NaCl in a Halotolerant Yeast, Pichia farinosa Cir A PDR5-\independent Pathway of Multi-Drug Resistance Regulated by the S/N4 Gene Product Fleckensteir Shall nd G YEAST SEQUENCING REPORTS Over-Expression of Candida albicans Mitochondrial Ribosomal Protein S9 (MrpS9p) Disturbs Mitochon- drial Function in Saccharomyces cerevisiae vvN ilts ¢ > B 17K a iA P YEAST FUNCTIONAL ANALYSIS REPORTS Disruption of Six Novel Saccharomyces cerevisiae Genes Reveals that YGL7129c is Necessary for Growth in Non-Fermentable Carbon Sources, YGL128c for Growth at Low or High Temperatures and YGL725w is implicated in the Biosynthesis of Methionine B.T A. Rodrig Torre d M. E. Cerdar 145 Disruption of Six Unknown Open Reading Frames from Saccharomyces cerevisiae Reveals Two Genes Involved in Vacuolar Morphogenesis and One Gene Required for Sporulation A } Santos, C. R. Vazquez de Aidana and J. L. R 15§ Disruption and Basic Phenotypic Analysis of Eighteen Novel Genes from the Yeast Saccharomyces cerevisiae f A y A Kerct . 1 Exaerd nd FF ry 165 CURRENT AWARENESS ON YEAST 173 Characterization of the Hansenula polymorpha CPY Gene Encoding Carboxypeptidase Y R Ff K K B. Rechinaer. M. Yav M ant - A KW. Kie 121 Dynamic Responses of Reserve Carbchydrate Metabolism Under Carbon and Nitrogen Limitations in Saccharomyces cerevisiae D., f " " , c Ba B sonza { i 101 Spontaneous Mutation, Oxidative DNA Damage, and the Roles of Base and Nucleotide Excision Repair in the Yeast Saccharomyces cerevisiae YEAST FUNCTIONAL ANALYSIS REPORTS Systematic Identification, Classification and Characterization of the Open Reading Frames which Encode Novel Helicase-related Proteins in Saccharomyces cerevisiae by Gene Disruption and Northern Analysis ’ } \A \ EF hia ka Y. Muraka iT Ek 19 YEAST SEQUENCING REPORTS Analysis of TFIIH Subunit, Through Isolation of the Gene from Schizosaccharomyces pombe Correspond ing to that of Saccharomyces cerevisiae SSL1, Reveals the Presence of Conserved Structural Motifs CURRENT AWARENESS ON YEAST 2¢ A 2-um DNA-Based Marker Recycling System for Multiple Gene Disruption in the Yeast Saccharomyces cerevisiae Yeast Vitality During Cider Fermentation: Assessment by Energy Metabolism identification and Characterization of Individual Cyclin-Dependent Kinase Complexes from Saccharo myces cerevisiae Cloning, Targeted Disruption and Heterologous Expression of the K/uyveromyces marxianus Endopoly galacturonase Gene (EPG7) YEAST SEQUENCING REPORTS Isolation and Sequence of the GCR3 Homologue from Candida albicans by Complementation of Agcr3 Strain of Saccharomyces cerevisiae YEAST FUNCTIONAL ANALYSIS REPORTS Transcript Analysis of 250 Novel Yeast Genes from Chromosome XIV Basic Phenotypic Analysis of Six Novel Yeast Genes Reveals Two Essential Genes and One which Affects the Growth Rate ISSUE No. 5, 30 MARCH 1999 Regulation of the Expression of the Kluyveromyces lactis PDC? Gene: Carbon Source-Responsive Elements and Autoregulation M. Destruelle, R. Menghini, L. Frontali and M. M. Bianchi 361 A Single Nucleotide Change in the MNA7 (VCX7/HUM7) Gene Determines Resistance to Manganese in Saccharomyces cerevisiae L. del Pozo, L. Osaba, J. Corchero and A. Jimenez Characterization of the Histidine Mutants of K/uyveromyces /actis B. J. M. Zonneveld, L. Tizzani and M. Wésolowski-Louvel Biosynthesis of Phytochelatins in the Fission Yeast. Phytochelatin Synthesis: A Second Role for the Glutathione Synthetase Gene of Schizosaccharomyces pombe A. Al-Lahham, V. Rohde, P. Heim, R. Leuchter, J. Veeck, C. Wunderlich, K. Wolf and M. Zimmermann Initial Characterization of the Nascent Polypeptide-Associated Complex in Yeast B. Reimann, J. Bradsher, J. Franke, E. Hartmann, M. Wiedmann, S. Prehn and B. Wiedmann YEAST SEQUENCING REPORTS Isolation and Characterization of the AF72 Gene Encoding Alcohol Acetyitransferase II in the Bottom Fermenting Yeast Saccharomyces pastorianus H. Yoshimoto, D. Fujiwara, T. Momma, K. Tanaka, H. Sone, N. Nagasawa and Y. Tamai 409 DNA Sequencing and Analysis of a 40 kb Region from the Right Arm of Chromosome II from Schizo- saccharomyces pombe M. Sanchez, F. del Rey, A. Dominguez, S. Moreno and J. L. Revuelta 419 YEAST FUNCTIONAL ANALYSIS REPORTS The Open Reading Frame YALO48c Affects the Secretion of Proteinase A in S. cerevisiae A. M. Wolff, J. G. L. Petersen, T. Nilsson-Tillgren and N. Din Disruption and Phenotypic Analysis of Seven ORFs from the Left Arm of Chromosome XV of Saccharo- myces cerevisiae K. Hajji, J. Clotet and J. Arino 435 ISSUE No. 6 APRIL 1999 Functional Analysis of HO Gene in Delayed Homothallism in Saccharomyces cerevisiae wy2 K. Ekino, |. Kwon, M. Goto, S. Yoshino and K. Furukawa Two-Dimensional Analysis of Proteins Secreted by Saccharomyces cerevisiae Regenerating Protoplasts A Novel Approach to Study the Cell Wall M. Pardo, L. Monteoliva, J. Pla, M. Sanchez, C. Gil and C. Nombela 459 Fzf1p of Saccharomyces cerevisiae is a Positive Regulator of SSU7 Transcription and its First Zinc Finger Region is Required for DNA Binding D. Avram, M. Leid and A. T. Bakalinsky Analysis of Genetic Interactions Between DHH1, SSD17 and ELM17 Indicates their Involvement in Cellular Morphology Determination in Saccharomyces cerevisiae H. Moriya and K. Ilsono 481 YEAST SEQUENCING REPORTS Schizosaccharomyces pombe stt3* is a Functional Homologue of Saccharomyces cerevisiae STT3 which Regulates Oligosaccharyltransferase Activity S. Yoshida, A. Matsuura, J. Merregaert and Y. Anraku YEAST FUNCTIONAL ANALYSIS REPORTS Heterologous URA3MX Cassettes for Gene Replacement in Saccharomyces cerevisiae A. L. Goldstein, X. Pan and J. H. McCusker How to Bring Orphan Genes into Functional Families M.M. Bianchi, G. Sartori, M. Vandenbol, A. Kaniak, D. Uccelletti, C. Mazzoni, J.-P. di Rago, G. Carignani, P. P. Slonimski ind L. Frontal 513 A Rapid Technique for the Determination of Unknown Plasmid Library Insert DNA Sequence Directly from Intact Yeast Cells E. R. Klebanow and P. A. Wei CURRENT AWARENESS ON YEAST Vectors for Rapid Selection of Integrants with Different Plasmid Copy Numbers in the Yeast Hansenula polymorpha DL1 AA "7 > Effects of Weak Acids on Cation Accumulation, ApH and A in Yeast Disruption of the KEX1 Gene in Pichia pastoris Allows Expression of Full-Length Murine and Human Endostatin ; f kk 562 Regulated Expression of the Saccharomyces cerevisiae Frelp/Fre2p Fe/Cu Reductase Related Genes YEAST SEQUENCING REPORTS identificatior f a Gene Encoding the Pyruvate Decarboxylase Gene Regulator CaPdc2p from Candida The K/IPMRTI Gene of Kluyveromyces /actis Encodes for a P-type Ca ATPase 9 YEAST FUNCTIONAL ANALYSIS REPORTS Systematic Analy f Yeast Strains with Possible Defects in Lipid Metabolism 2 senerat f Null Alleles for the Functional Analysis of Six Genes from the Right Arm of Saccharomyces f ne | ‘ ve Il HF RRENT AWARENESS ON YEAST pe F t Re etwee Cod Usage Bias and Translation Initiation AUG Context in . ny - ‘ , ‘ , SS is by the Fission Yeast Schizosaccharomyces pombe f ter dal Yeast Strains by Expressing the Pedi »ccus acidilactici Pediocin Gene P| ere ; Le ‘ 7 tase f the Ma e V< t Debaryomyces hanseni . r N , € Schi: . haromyces pombe Required for Eff ent Cell Separaatndi Soexnua l f T Y A " ‘ ; MV ) Mie t me Hybridization C C \ r j JALYSIS REPORTS Cc NA } ‘ H is ten f Ne ff ts Re t , F Ele ste Leve f the Re I itional Repair Protein Rad54p } 7 Manse } y ha PDD! Gene Product, Essential for the Selective Degradation of Peroxisomes Int Hon ‘ Pa ms vce ere\ 2e Vps34) Fu Characteristics of the Heterologously Expressed Human Lanosterol 14a-Demethylase (Other Names; P45014DM, CYP51, P45051) and Inhibition of the Purified Human and Candida albicans CYP51 with Azole Antifungal Agents D. C. Lamb, D. E. Kelly, M. R. Waterman, M. Stromstedt, D. Rozman and S. L. Kelly Combining Mutations in the Incoming and Outgoing Pheromone Signal Pathways Causes a Synergistic Mating Defect in Saccharomyces cerevisiae L. Giot, C. DeMattei and J. B. Konopka 765 Linear DNA Plasmid pPK2 of Pichia kluyveri: Distinction Between Cytoplasmic and Mitochondrial Linear Piasmids in Yeasts J. Blaisonneau, J. Nosek and H. Fukuhara YEAST SEQUENCING REPORTS Cloning of a Centromere Binding Factor 3d (CBF3D) Gene From Candida glabrata T. Stoyan, R. Eck, J. Lechner, P. Hemmerich, W. Kunkel and S. Diekman 793 Isolation of a Histoplasma capsulatum cDNA that Complements a Mitochondrial NAD(+)-lsocitrate Dehydrogenase Subunit I-deficient Mutant of Saccharomyces cerevisiae C. H. Johnson and J. E. McEwen 799 CURRENT AWARENESS ON YEAST 805 SSUE No. 10A JULY 1999 Role of NaOQH-Extractable Cell Wall Proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (Members of the Pir Protein Family) in Stability of the Saccharomyces cerevisiae Cell Wall Mréa and W. Tanner o21t2] The Topoisomerase | Poison Camptothecin Generates a Chk1-dependent DNA Damage Checkpoint Signal in Fission Yeast S. Wan, H. Capasso and N. C. Walworth O2<9 4 A Proposal for Nomenclature of Aldehyde Dehydrogenases in Saccharomyces cerevisiae and Characteriz- ation of the Stress-inducible ALD2 and ALD3 Genes P. Navarro-Avino, R. Prasad J. Miralles, R. M. Benito and R. Serranc 829 Overproduction of the Opil Repressor Inhibits Transcriptional Activation of Structural Genes Required for Phospholipid Biosynthesis in the Yeast Saccharomyces cerevisiae C. Wagner, M. Blank, B. Strohmanannd H.-J. Schuller Deletion of the Carbonic Anhydrase-like Gene NCE103 of the Yeast Saccharomyces cerevisiae Causes an Guygen sensitive Growth pares ( A. Gnann and F. K. Zir i A pean womencteture for New Translation Initiation Factor Genes from S. pombe and other Fungi der. H.-P. Vornloche ) W. B. Hersanhd eJ. yE. G. McCarthy The Three Copies of the A7P7 Gene are Arranged in Tandem on Chromosome Il of Saccharomyces cerevisiae $288C ikeda. H. Satoh. K. Of} st T. Satoh and T. Mabuchi Three » Genes Whose Expression is Induced by Stress in Saccharomyces cerevisiae Arroyo and A.A. C yVvarruDias YEAST SEQUENCING REPORTS DNA Sequencing and Analysis of a 67-4 kb Region from the Right Arm of Schizosaccharomyces pombe Chromosome I! Reveals 28 Open Reading Frames Including the Genes his5, pol, ppa2, rip?, rpb& and skb? Xiang, M. H. Lyne, V. Wood, M.-A. Rajandream, B. G. Barrell and S. J. Av 893 CURRENT AWARENESS ON YEAST SUE Ne 10B JULY 1999 SPECIAL ISSUE Yeast Functional Analysis Reports Supported it Dy the Yeast Industry Platforn introduction—Yeast Industry Platform Func tional Analysis of 12 ORFs from Saccharomyces cerevisiae Chromosome Il yuez-Navarr Estandr J.u E. cPerehz-Or tir Disruption of Six Open Reading Frames on Chromosome X of Saccharomyces cerevisiae Reveals a Cluster of Four Essential Genes Disruption and Basic Functional Analysis of Six Novel ORFs of Chromosome XV from Saccharomyces cerevisiae I35 Disruption of Six Saccharomyces cerevisiae Novel Genes and Phenotypic Analysis of the Deletants : } 5 V ‘ ez P_G B Hiesta and M Remacha 345 Construction and Genetic Analysis of S. cerevisiae Deletants of Six Novel ORFs from Chromosome Il + M 1A \ Q Epitope Tagging of Yeast Genes Using a PCR-based Strategy: More Tags and Improved Practical Routines MK K ~ ) W. Zacharias B. Wins K. Nasmyth and & >chniebe 63 Chemotyping of Yeast Mutants Using Robotics . r M. Ef 4 i \ Bradshaw P_P >10T TSK ] Maundre j Disruption of Six Novel Yeast Genes Located on Chromosome II Reveals One Gene Essential for Vegetative Growth and Two Required for Sporulation and Conferring Hypersensitivity to Various Chemicals " yk AAV iigdalski, P. P. Stonimsk A Large-Scale Sonication Assay for Cell Wall Mutant Analysis in Yeast A. Fast Method to Diagnose Chromosome and Plasmid Loss in Saccharomyces cerevisiae Strains K ed Disruption of the Cytochrome c Gene in Xylose-utilizing Yeast Pichia stipitis Leads to Higher Ethanol Production Total Number of Coding Open Reading Frames in the Yeast Genome Analysis of the Peroxisomal Acy!-CoA Oxidase Gene Product from Pichia pastoris and Determination of its Targeting Signal Transcription of the HXT4 Gene is Regulated by Gcr1p and Gcr2p in the Yeast S. cerevisiae Hansenula Polymorpha Pex1p and Pex6p are Peroxisome-associated AAA Proteins that Functionally and Physically Interact Distributior f the Actin Cytoskeleton During the Cell (¢ le f Yarrowia lipolytica and the Visualization f the Tubulin Cytoskeleton by Immunofluorescence Hiagh-yield Secret f f Recombinant Gelatins by Pichia pastor Jeletior f SF/7T, a Novel Suppressor of Partial Ras-cAMP Pathway Deficiency in the Yeast Saccharo ce erevisiae, Causes G. Arrest t in the Sa haromyces cerevisiae Gene SAC 2use Multiple Drug-Sensitivity Schizosaccharomyces pombe Homologue of Glutathione Peroxidase vi ' foes not Contain Seleno ced by Several Stresseasn d Works as an Antioxidant Mu YEAST SEQUENCING REPORTS Kin Molecular Clonir J f Homologs of RAS and RHOT Genes from Crypté« occus neoformans ade YEAST FUNCTIONAL ANALYSIS REPORTS Mor Designer Deletion and Prototrophic Strains Derived from Saccharomyces cerevisiae Strain W303-1la Cyst CURRENT AWARENESS ON YEAST SSUE No. 12, 15 SEPTEMBER 1999 Saccharomyces cerevisiae Colony Growth and Ageing: Biphasic Growth Accompanied by Changes in Gene Expression J.-R. Meunier and M. Choder A Mutant for the Yeast scERV? Gene Displays a New Defect in Mitochondrial Morphology and Distribution D. Becher, J. Kricke, G. Stein and T. L wsky Molecular Mechanism of the Multiple Regulation of the Saccharomyces cerevisiae ATF1 Gene Encoding Alcohol Acetyltransferase D Fujiv ara, O Kobayashi, H. Yoshimoto S. Harashima and Y. Tam Con Cycle Regulation of a DNA Ligase- epee Gene (CalIG4) from Candida albicans Andaluz, A. Ciudad, J. R. Coque, R. Calderone and G. Larriba Endocytosis and Vacuolar Morphology in Saccharomyces cerevisiae Are Altered in Response to Ethanol Stress or Heat =e Meaden. N ebora, L Guldfeldt, H. Siequmfeldt and M. Jakobser Alcohol Oxidese Hybrid retain Formed /n Vivo and /n Vitro Nakagawa, H. Mukaiyama, H. Yurimoto, Y. Sakai and N. Kat Gene Senkation in Schizosaccharomyces pombe Using a Temperature-sensitive Ura4p K. Davis, C. Pateman and J. Davey YEAST SEQUENCING REPORTS Isolation and Expression of the Gene Encoding Mitochondrial ADP/ATP Carrier (AAC) from the Pathogenic Yeast Candide parapsilosis Vi. Nebohaéova, M Mar J. Nosek and J. Kolar identification of Putative Response Regulator Two-component Phosphorelay Gene (CaSSK7) from Candida albicans A. Ldlelda a nadR A Calder YEAST FUNCTIONAL ANALYSIS REPORTS Disruption and Functional Analysis of Seven ORFs on Chromosome IV: YDLO57w, YDLO12c, YDLO10w, VOUCSCe. YOLOOSw (APCT11), YDLOOSc (MED2) and YDLOO3w (MCD7) KN L. iwanejko, S. Los F. Fabarnd eA. N A Strong Carbon Source Effect is Mediated by puC Plasmid Sequences in a Series of Classical Yeast Vectors Designed for Promoter Characterization A. Abadiieva. PG . Hilven and yM.y Crabee Deletion of Six shinies Reading Frames from the Left Arm of Chromosome IV of Saccharomyces cerevisiae T r, B. Prein, A drositzG,. Daun 1S.D.K Disruption of Six Novel ORFs on the Left Arm of Chromosome XIil Reveals One Gene Essential for Vegetative Growth of Saccharomyces cerevisiae x A var [ sardner. R._ N Walmsle ERRATUM Construction of a Lactose-assimilating Strain of Baker's Yeast R A Selection System for Human Apoptosis Inhibitors Using Yeast One-step, PCR-mediated, Gene Disruption in the Yeast Hansenula polymorpha Perdon p > 7 ) a ¥. f©Rc r it a} nd } AA\ Sivve r Mutations that Cause Threonine Sensitivity Identify Catalytic and Regulatory Regions of the Aspartate Kinase of Saccharomyces cerevisiae ade9 is an Allele of SER? and Plays an Indirect Role in Purine Biosynthesis RDp f< Mouse CAF1, a Mouse Homologue of the Yeast POP2 Gene Complements the Yeast pop2 Null Mutation M. , Sasar A | Y hioka, AA . AMAaatts ur » and AA . Saka Cysteine Besynthenis 5i n Saccharomyc es cerevisiae: a New Outlook on Pathway and Regulation S ] Kawa ‘2 la Brzvweczy and A Paszewsk 1365 Signalling between Mitochondria and the Nucleus Regulates the Expression of a New po-Lactate Dehy drogenase Activity in Yeast nberg and R. A. Butow YEAST SEQUENCING REPORTS The URA5S Gene Encoding Orotate-phosphoribosyl Transferase of the Yeast K/uyveromyces /actis Cloning, Sequencing and Use as a Selectable Marker Molecular Cloning and Sequence Analysis of Zygosaccharomyces rouxii ADE2 Gene Encoding a Phosphoribosy!-aminoimidazole Carboxylase Cloning and Characterization of the K/uyveromyces lactis Homocysteine Synthase Gene YEAST FUNCTIONAL ANALYSIS REPORTS Disruption of Six ORFs on Saccharomyces cerevisiae Chromosome X: the YJL069c Gene of Unknown Function is Essential to Cell Viability High-efficiency Gene Targeting in Schizosaccharomyces pombe Using a Modular, PCR-based Approach with Long Tracts of Fianking Homology CURRENT AWARENESS ON YEAST The Proteolyt Systen f the Yeast K/uyveromyces /actis Role of the Sulphate Assimilation Pathway in Utilization of Glutathione as a Sulphur Source by Sa haromyces cerevisiae The Yeast PRS3 Gene is Required for Ceili Integrity, Cell Cycle Arrest upon Nutrient Deprivation, lon Homeostasis and the Proper Organization of the Actin Cytoskeleton STRE- and cAMP-independent Transcriptional Induction of Saccharomyces cerevisiae GSY2 Encoding Glycogen Synthase Dur g Diauxic Growth on Glucose A Novel Vacuolar Protein Encoded by SSU27/MCD4 is Involved in Cell Wall Integrity in Yeast 5-— Allelisn »f Saccharomyces cerevisiae Genes PSO6. Involved in Survival After 3-CPs+UVA Induced Damage. and ERG Er »ding the Enzyme Stero! C-5 Desaturase ™T*r Replicative Ageing in the Fission Yeast Schizosaccharomyces pombe ww YEAST SEQUENCING REPORTS Sequence Analy f SLA2 of the Dimorphic Yeasts Candida albicans and Yarrowia lipolytica YEAST FUNCTIONAL ANALYSIS REPORTS Disruption and Functional Analysis of Six ORFs on Chromosome XV YOL117w. YOL115w TRF4) YOL114c, YOL112w (MS84), YOL111c and YOLO72w Three New Dominant Drug Resistance Cassettes for Gene Disruption in Saccharomyces cerevisiae Lipid Composition of Subcellular Membranes of an FY1679-derived Haploid Yeast Wild-type Strain Grown yn Different Carbon Sources CURRENT AWARENESS ON YEAST ISSUE No. 15 NOVEMBER 1999 Molecular Cloning and Characterization of the Gene HXK71 Encoding the Hexokinase from Yarrowia lipolytica T. Petit and C. Gancedo An Alternative Model for the Transmembrane Segments of the Yeast H* -ATPase J. P. Pardo, F. Martinez, G. Guerra, |. Velazquez, J. L. Rendén and G. Mendoza FLR1 Gene (ORF YBROO8c) is Required for Benomyl and Methotrexate Resistance in Saccharomyces cerevisiae and its Benomyl-induced Expression is Dependent on Pdr3 Transcriptional Regulator N. Bréco, S. Tenreiro, C. A. Viegas and |. Sa-Correia Transformation of Candida albicans by Electroporation M. D. De Backer, D. Maes, S. Vandoninck, M. Logghe, R. Contreras and W. H. M. L. Luyten 1 609 A Potential Role of the Cytoskeleton of Saccharomyces cerevisiae in a Functional Organization of Glycolytic Enzymes R. Gotz, E. Schluter, G. Shoham andF . K. Zimmermann 1 619 Cloning and Characterization of the EXG7 Gene from the Yeast Yarrowia /lipolytica P. F. Esteban, S. Casarégola, C. R. Vazquezd e Aldana and F. del Rey 1 631 Trans-dominant Mutations in the GPR? Gene Cause High Sensitivity to Acetic Acid and Ethanol in the Yeast Yarrowia lipolytica K. Tzschoppe, A. Augstein, R. Bauer, S. D. Kohlwein and G. Barth 1 645 inositol Synthesis and Catabolism in Cryptococcus neoformans Y. Molina, S. E. Ramos, T. Douglass and L. S. Klig 4 657 YEAST FUNCTIONAL ANALYSIS REPORTS A Series of Protein Phosphatase Gene Disruptants in Saccharomyces cerevisiae N. Sakumoto, Y. Mukai, K. Uchida, T. Kouchi, J. Kuwajima, Y. Nakagawa, S. Sugioka, E. Yamamoto, T. Furuyama H. Mizubuchi, N. Ohsugi, T. Sakuno, K. Kikuchi, |. Matsuoka, N. Ogawa, Y. Kaneko and S. Harashima 4 669 Disruption of Seven Hypothetical Ary! Alcohol Dehydrogenase Genes from Saccharomyces cerevisiae and Construction of a Multiple Knock-Out Strain D. Delneri, D. C. J. Gardner, C. V. Bruschi and S. G. Oliver 1681 Disruption and Phenotypic Analysis of Six Novel Genes from Chromosome !V of Saccharomyces cerevisiae Reveal YDLO60w as an Essential Gene for Vegetative Growth E. Casalone, C. Barberio, D. Cavalieri, |. Ceccarelli, M. Riparbelli, S. Ugolini and M. Polsinell 41 691 CURRENT AWARENESS ON YEAST 1703 ISSUE No. 16 DECEMBER 1999 Transcription of Some PHO Genes in Saccharomyces cerevisiae is Regulated by Spt7p K. Nishimura, K. Yasumura, K. Igarashi, S. Harashima and Y. Kakinuma RGD! Genetically Interacts with M/D2 and SLG7, Encoding Two Putative Sensors for Cell Integrity Sgnemny in Saccharomyces cerevisiae je Bettiqnies, C. Barthe, C. Morel, M. F. Peypouquet, F. Doignon and M. Crouzet YEAST FUNCTIONAt ANALYSIS REPORTS Selection of Genes Repressed by cAMP that are Induced by Nutritional Limitation in Saccharomyces c erevisiae Tadi, R. N. Hasan, F. Bussereau, E. Boy-Marcotte and M. Jacquet Facilitating Functional Analysis of the Saccharomyces cerevisiae Genome Using an EGFP Based Promoter Library and Flow Cytometry PL. Bell, |. W. Davies and P. V. Attfield New Tools for Protein Linkage Mapping and General Two-hybrid Screening T. Durfee, O. Draper, J. Zupan, D. S. Conklin and P. C. Zambrysk 761 The trp1-AFA Designer Deletion for PCR-based Gene Functional Analysis in Saccharomyces cerevisiae Horecka and Y. Jigami 1 769 Systematic Analysis of S. cerevisiae Chromosome VIII Genes R. Niedenthal, L. Riles, U. Guldener, S. Klein, M. Johnston and J. H. Hegemann YEAST SEQUENCING REPORTS isolation and Characterization of MELt Gene from Torulaspora delbrueckii \FO 1255 Oda and M. Fukunaga Calendar Author Index Key Word Index Volume Contents