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Contents VOLUME 14, ISSUE Nos. 1-16 Yeast ISSUE No. 1, 15 JANUARY 1998 Sequences of Saccharomyces cerevisiae 24m DNA Improving Plasmid Partitioning in Hansenula polymorpha A. |. Bogdanova, O. S. Kustikova, M. O. Agaphonov and M. D. Ter-Avanesyan Development of the Methylotrophic Yeast Pichia methanolica for the Expression of the 65 Kilodalton isoform of Human Glutamate Decarboxylase C. K. Raymond, T. Bukowski, S. D. Holderman, A. F. T. Ching, E. Vanaja and M. R. Stamm Distribution of the Flocculation Protein, Flop, at the Cell Surface During Yeast Growth: The Availability of Flop Determines the Flocculation Level M. Bony, P. Barre and B. Blondin The Lysine-rich C-terminal Repeats of the Centromere-binding Factor 5 (Cbf5) of Kluyveromyces /actis are not Essential for Function A. A. Winkler, A. Bobok, B. M. Zonneveld, H. Y. Steensma and P. J. J. Hooykaas The Sge1 Protein of Saccharomyces cerevisiae is a Membrane-associated Multidrug Transporter A. E. Ehrenhofer-Murray, M. U. Keller Seitz and C. Sengstag Engineering Yeast for Efficient Cellulose Degradation P. van Rensburg, W. H. van Zyl and| . S. Pretorius Kluyveromyces factis SEF? and its Saccharomyces cerevisiae Homologue Bypass the Unknown Essential Function, but not the Mitochondrial RNase P Function, of the S. cerevisiae RPM2 Gene K. R. Groom, H. C. Heyman, M. C. Steffen, L. Hawkins and N. C. Martin YEAST FUNCTIONAL ANALYSIS REPORTS Efficient PCR-based Random Mutagenesis of Sub-genic (100 bp) DNA Fragments V. Svetiov and T. G. Cooper CURRENT AWARENESS ON YEAST ERRATUM ISSUE No. 2, 30 JANUARY 1998 Mutations in Five Loci Affecting GAP7-independent Uptake of Neutral Amino Acids in Yeast M. U. Jorgensen, M. B. Bruun, T. Didion and M. C. Kielland-Brandt Designer Deletion Strains Derived from Saccharomyces cerevisiae S288C: a Useful Set of Strains and Plasmids for PCR-mediated Gene Disruption and Other Applications C. B. Brachmann, A. Davies, G. J. Cost, E. Caputo, J. Li, P. Hieter and J. D. Boeke Mutations of the CDC28 Gene and the Radiation Sensitivity of Saccharomyces cerevisiae N. A. Koltovaya, |. P. Arman and A. B. Devin Flow Cytometry and Cell Sorting for Yeast Viability Assessment and Cell Selection D. Deere, J. Shen, G. Vesey, P. Bell, P. Bissinger and D. Veal Disruption of the Saccharomyces cerevisiae YAP3 Gene Reduces the Proteolytic Degradation of Secreted Recombinant Human Albumin S. M. Kerry-Williams, S. C. Gilbert, L. R. Evans and D. J. Ballance Functional Comparison of the Yeast scERV7 and scERV2 Genes G. Stein and T. Lisowsky YEAST SEQUENCING REPORTS Molecular Cloning and Sequencing of a Chitin Synthase Gene (CHS2) of Paracoccidioides brasiliensis G. A. Nifio-Vega, E. T. Buurman, G. W. Gooday, G. San-Blas and N. A. R. Gow Characterization of a Branched-chain Amino-acid Aminotransferase from Schizosaccharomyces pombe A. Eden and N. Benvenisty ISSUE No. 3 FEBRUARY 1998 Investigation of Two Yeast Genes Encoding Putative lsoenzymes of Phosphoglycerate Mutase J. J. Heinisch, S. Muller, E. Schlutter, J.J acoby and R. Rodicio Post-translational Fate of CAN7 Permease of Saccharomyces cerevisiae M. Opekarova, T. Caspari, B. Pinson, D. Bréthes and W. Tanner The Saccharomyces cerevisiae TGL2 Gene Encodes a Protein with Lipolytic Activity and can Complement an Escherichia coli Diacylglycerol Kinase Disruptant G. P. H. van Heusden, M. Nebohacova, T. A. Overbeeke and H. Y. Steensma isolation and Characteristics of Yeasts able to Grow at Low Concentrations of Nutrients Y. Kimura, Y. Nakano, K. Fujita, S. Miyabe, S. Imasaka, Y. Ishikawa and M. Sato identification and Kinetic Analysis of a Functional Homelog of Elongation Factor 3 YEF3 in Saccharo- myces cerevisiae A. V. Sarthy, T. McGonigal, J. O. Capobianco, M. Schmidt, S. R. Green, C. M. Moehle and R. C. Goldman During the Initiation of Fermentation Overexpression of Hexokinase Pll in Yeast Transiently causes a Similar Deregulation of Glycolysis as Deletion of Tps1 J). R. Ernandes, C. De Meirsman, F. Rolland, J. Winderickx, J. de Winde, R. L. Brandao and J. M. Thevelein YEAST FUNCTIONAL ANALYSIS REPORTS Generation of Saccharomyces cerevisiae Deletants and Basic Phenotypic Analysis of Eight Novel Genes from the Left arm of Chromosome XIV M. Maftahi, C. Gaillardin and J. Nicaud YEAST SEQUENCING REPORTS Cloning, Sequencing and Disruption of the ARG& Gene Encoding Acetylornithine Aminotransferase in the Petite-negative Yeast K/uyveromyces /actis A. Janssen and X. J. Chen The SPL7 tRNA Splicing Gene of Candida maltosa and Candida albicans E. P. Plant, D. Becher and R. T. M. Poulter CURRENT AWARENESS ON YEAST ISSUE No. 4, 15 MARCH 1998 Mam33p, an Oligomeric, Acidic Protein in the Mitochondrial Matrix of Saccharomyces cerevisiae is Related to the Human Complement Receptor gC1q-R T. Seytter, F. Lottspeich, W. Neupert and E. Schwarz Synthesis of Monohydroxylated Inositolphosphoryiceramide (IPC-C) in Saccharomyces cerevisiae Requires Scs7p, a Protein with both a Cytochrome b,-like Domain and Hydroxylase/desaturase Domain T. M. Dunn, D. Haak, E. Monaghan and T. J. Beeler Assembly of Phosphofructokinase-1 from Saccharomyces cerevisiae in Extracts of Single-Deletion Mutants A. Klinder, J. Kirchberger, A. Edelmanann d G. Kopperschlager Purified Arginine Permease of Candida albicans is Functionally Active in a Reconstituted System P. K. Mukherjee and R. Prasad The Importance of the Glycerol 3-Phosphate Shuttle During Aerobic Growth of Saccharomyces cerevisiae C. Larsson, |.-L. Pahiman, R. Ansel!, M. Rigoulet, L. Adler and L. Gustafsson The SKS71 Gene of Saccharomyces cerevisiae is Required for Long-term Adaptation of snf3 Null Strains to Low Glucose P. Vagnoli and L. F. Bissor Glycosylation of Human a,-Antitrypsin in Saccharomyces cerevisiae and Methylotrophic Yeasts H. A. Kang, J.-H. Sohn, E.-S. Choi, B. H. Chang, M.-H. Yu and S.-K. Rhee Purification and Properties of Polyphosphatase from Saccharomyces cerevisiae Cystosol N. Andreeva, T. Kulakovskaya, |. Sidorov, A. Karpov and |. Kulaev YEAST FUNCTIONAL ANALYSIS REPORTS Construction of a PCR-ligated Long Flanking Homology Cassettes for Use in the Functional Analysis of Six Unknown Open Reading Frames from the Left and Right Arms of Saccharomyces cerevisiae Chromosome XV B. M. Pearson, Y. Hernandaon d M. Schweizer ISSUE No. 5, 30 MARCH 1998 Isolation and Characterization of K/uyveromyces marxianus Mutants Deficient in Malate Transport O. Queirés, M. Casal, S. Althoff, P. Moradas-Ferreira and C. Leao Identification and Analysis of Homologues of Saccharomyces cerevisiae Spt3 Suggest Conserved Functional Domains J. M. Madison and F. Winston Heat Shock Transiently Enhances the Synthesis Rate of Sisip, a Ribosome-Associated DnaJ Protein in the Oleagenous Yeast Apiotrichum curvatum V. Specht, M. Lubeck and H. Kindl Application of mRNA Differential Display to Investigate Gene Expression in Thermotolerant Cells of Saccharomyces cerevisiae C. Gross and K. Watson Evolution of Gene Order and Chromosome Number in Saccharomyces, Kluyveromyces and Related Fungi R. S. Keogh, C. Seoighe and K. H. Wolfe Regulation of Alcoholic Fermentation in Batch and Chemostat Cultures of Kluyveromyces /actis CBS 2359 J. Kiers, A.-M. Zeeman, M. Luttik, C. Thiele, J. |. Castrillo, H. Y. Steensma, J. P. van Dijken and J. T. Pronk YEAST FUNCTIONAL ANALYSIS REPORTS The List of Cytoplasmic Ribosomal Proteins of Saccharomyces cerevisiae R. J. Planta andW . H. Mager YEAST SEQUENCING REPORTS Nucleotide Sequence of the Schizosaccharomyces pombe lys1+ Gene and Similarities of the lys1+ Protein to Peptide Antibiotic Synthetases V. Bhattacherjee and J. K. Bhattacharjee Characterization of the Prk1 Protein Kinase from Schizosaccharomyces pombe P. Watson and J. Davey CURRENT AWARENESS ON YEAST ISSUE No. 6 APRIL 1998 Influence of Monovalent Cations on Yeast Cytoplasmic and Vacuolar pH M. Calahorra, G. A. Martinez, A. Hernandez-Cruz and A. Pena Induced Expression of the Candida albicans Multidrug Resistance Gene CDA7 in Response to Fluconazole and Other Antifungals M. L. Hernaez, C. Gil, J. Pla and C. Nombela Identification of YHRO719 in Saccharomyces cerevisiae Chromosome VIII as the Gene for the Cytosolic Asparaginyl-tRNA Synthetase |. Landrieu, M. Vandenbol, R. Leberman, D. Portetellaen d M. Hartlein Deletion of Transmembrane Domain 12 of CDA7, a Multidrug Transporter from Candida albicans, Leads to Altered Drug Specificity: Expression of a Yeast Multidrug Transporter in Baculovirus Expression System S. Krishnamurthy, U. Chatterjee, V. Gupta, R. Prasad, P. Das, P. Snehlata, S. E. Hasnain and R. Prasad A Family of Laboratory Strains of Saccharomyces cerevisiae Carry Rearrangements Involving Chromosomes | and !"! S. Casaregola, H. Vang Nguyen, A. Lepingle, P. Brignon, F. Gendre and C. Gaillardin YEAST FUNCTIONAL ANALYSIS REPORTS An Improved Protocal for the Preparation of Yeast Cells for Transformation by Electroporation J. R. Thompson, E. Register, J. Curotto, M. Kurtz and R. Kelly YEAST SEQUENCING REPORTS Cloning and Sequencing of a cDNA Encoding a Copper-Zinc Superoxide Dismutase Enzyme from the Marine Yeast Debaryomyces hansenii N. Y. Hernandez-Saavedra, J. M. Egly and J. L. Achoa Homolog of a-factor Receptor Gene in Saccharomyces exiguus K.-1. Kusumoto, A. Hino, Y. Yonekura, Y. Nogata, H. Ohta and H. Takano A 9359 bp Fragment from the Right Arm of Saccharomyces cerevisiae Chromosome VII Includes the FOL2 and YTA7 Genes and Three Unknown Open Reading Frames M. L. Agostoni Carbone, G. Lucchini, P. Melchioretto, V. Nardese, M. Vanoni and L. Panzeri CALENDAR CURRENT AWARENESS ON YEAST ISSUE No. 7 MAY 1998 Physical Mapping of Chromosomes VIi and XV of Saccharomyces cerevisiae at 3.5kb Average Resolution to Allow their Complete Sequencing H. Tettelin, A. Thierry, A. Goffeau and B. Dujon An Extracellular Meiosis-promoting Factor in Saccharomyces cerevisiae M. Hayashi, K. Ohkunanid |. Yamashita Bicarbonate-mediated Social Communication Stimulates Meiosis and Sporulation of Saccharomyces cerevisiae K. Ohkuni, M. Hayashanid |. Yamashita The Saccharomyces cerevisiae Early Secretion Mutant tip20 is Synthetic Lethal with Mutants in Yeast Coatomer and the SNARE Proteins Sec22p and Ufelp G. Frigerio isolation of the Pichia pastoris PYC1 Gene Encoding Pyruvate Carboxylase and Identification of a Suppressor of the pyc Phenotype J. Menendez, J. Delgado and C. Gancedo YEAST FUNCTIONAL ANALYSIS REPORTS Genomic Disruption of Six Budding Yeast Genes gives one Drastic Example of Phenotype Strain-dependence E. Bilsland, M. Dahlén and P. Sunnerhagen YEAST SEQUENCING REPORTS identification of a Putative Histidine Kinase Two-component Phosphorelay Gene (CaHK7) in Candida albicans J. A. Calera, G.H. Choi and R. A. Calderone Isolation and Characterization of the Candida albicans SEC4 Gene M. Clément, H. Fournier, L. de Repentigny and P. Belhumeur Genetic Organization and Sequence Analysis of the Hypha-specific Cell Wall Protein Gene HWP7 of Candida albicans J. F. Staab and P. Sundstrom The H/S4 Gene from the Yeast K/luyveromyces /actis M. A. Freire-Picos, M. Hampsey and M. E. Cerdan CURRENT AWARENESS ON YEAST ISSUE No. 8, 15 JUNE 1998 Pombe: A Gene-finding and Exon-intron Structure Prediction System for Fission Yeast T. Chen and M. Q. Zhang Transcriptional Co-regulation of Saccharomyces cerevisiae Alcohol Acetylitransferase Gene, ATF7 and \-9 Fatty Acid Desaturase Gene, OLE7 by Unsaturated Fatty Acids D. Fujiwara, H. Yoshimoto, H. Sone, S. Harashima and Y. Tamai Comparison of the Structural Characteristics of Chromosome VI in Saccharomyces Sensu Stricto: The Divergence, Species-Dependent Features and Uniqueness of Saké Yeasts A. Nakazato, T. Kadokura, M. Amano, T. Harayama, Y. Murakami, M. Takeda, M. Ohkuma, T. Kudo andT . Kaneko The C-terminal Hydrophobic Repeat of Schizosaccharomyces pombe Heat Shock Factor is not Required for Heat-induced DNA-binding K. A. Saltsman, H. L. Prentice and R. E. Kingston Functional Analysis of the Saccharomyces cerevisiae UBC11 Gene F. M. Townsley and J. V. Ruderman The Pichia pastoris Dihydroxyacetone Kinase is a PTS1-containing, but Cytosolic, Protein that is Essential for Growth on Methanol G. H. Luers, R. Advani, T. Wenzel and S. Subramani N-Glycosylation by Transfer of GIcNAc, from Dolichol-PP-G1cNAc, to the Protein Moiety of the Major Yeast Exoglucanase R. Cueva, C. Cotano and G. Larriba YEAST FUNCTIONAL ANALYSIS REPORTS A Versatile Set of Vectors for Constitutive and Regulated Gene Expression in Pichia pastoris |. B. Sears, J. O'Connor, O. W. Rossanese and B. S. Glick CALENDAR ERRATUM... ISSUE No. 9, 30 JUNE 1998 A Novel Esterase from Saccharomyces carlsbergensis, a Possible Function for the Yeast 7/P7 Gene M. W. Horsted, E. S. Dey, S. Holmberg and M. C. Kielland-Brandt Development of a Transformation System for the Multinuclear Yeast Dipodascus (Endomyces) magnusii L. Adamikova, P. Griaé, L. TomaSka and J. Nosek Quantitative Imaging of TATA-Binding Protein in Living Yeast Cells G. H. Patterson, S. C. Schroeder, Y. Bai, P. A. Weil and D. W. Piston Highly Efficient Assimilation of Lactose by a Metabolically Engineered Strain of Saccharomyces cerevisiae M. Rubio-Texeira, J. |. Castrillo, A. C. Adam, U. O. Ugalde and J. Polaina ARH1 of Saccharomyces cerevisiae: A New Essential Gene That Codes for a Protein Homologous to the Human Adrenodoxin Reductase L. Manzella, M. H. Barros and F. G. Nobrega Curing Saccharomyces cerevisiae of the 2 Micron Plasmid by Targeted DNA Damage E. L. Tsalik and M. R. Gartenberg YEAST FUNCTIONAL ANALYSIS REPORTS The Deletion of Six ORFs of Unknown Function From Saccharomyces cerevisiae Chromosome VII Reveals Two Essential Genes: YGR795w and YGR198w J. M. Rodriguez-Pena, V. J. Cid, M. Sanchez, M. Molina, J. Arroyo and C. Nombela YEAST SEQUENCING REPORTS Cloning and Sequence Analysis of the Pichia pastoris TRP1, IPP1 and H/IS3 Genes 1. C. Cosano, P. Alvarez, M. Molina and C. Nombela identification and Characterization of the KLCMD7 Gene Encoding Kluyveromyces /actis Calmodulin T. F. Rayner and M. J. R. Stark CURRENT AWARENESS ON YEAST ISSUE No. 10 JULY 1998 Differentiation of Brewing Yeast Strains by Pyrolysis Mass Spectrometry and Fourier Transform infrared Spectroscopy E. M. Timmins, D. E. Quain and R. Goodacre Chromosomal DNA Patterns and Gene Stability of Pichia pastoris H. Ohi, N. Okazaki, S. Uno, M. Miura and R. Hiramatsu Control of Division Arrest and Entry into Meiosis by Extracellular Alkalisation in Saccharomyces cerevisiae M. Hayashi, K. Ohkuni and |. Yamashita Genetic Interaction Between YP76 and YPT7 in Saccharomyces cerevisiae B. Li and J. R. Warner Co-existence of Two Types of Chromosomes in the Bottom Fermenting Yeast, Saccharomyces pastorianus Y. Tamai, T. Momma, H. Yoshimoto and Y. Kaneko YEAST FUNCTIONAL ANALYSIS REPORTS Drug-induced Phenotypes Provide a Tool for the Functional Analysis of Yeast Genes H. Launhardt, A. Hinnen and T. Munder Heterologous Modules for Efficient and Versatile PCR-based Gene Targeting in Schizosaccharomyces pombe J. Bahler, J. Wu, M. S. Longtine, N. G. Shah, A. McKenzie Ill, A. B. Steever, A. Wach, P. Philippsen and J. R. Pringle Additional Modules for Versatile and Economical PCR-based Gene Deletion and Modification in Saccharomyces cerevisiae M. S. Longtine, A. McKenzie III, D. J. DeMarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen and J. R. Pringle YEAST SEQUENCING REPORTS isolation and Nucleotide Sequence of the Gene Encoding Phosphoenolpyruvate Carboxykinase from Kluyveromyces /actis H. K. Kitamoto, S. Ohmomo and Y. limura CURRENT AWARENESS ON YEAST ISSUE No. 11 AUGUST 1998 REVIEW The Structure and Function of Yeast Xylose (Aldose) Reductases H. Lee Multiple Regulatory Proteins Mediate Repression and Activation by Interaction With the Yeast Mig1 Binding Site J. Wu and R. J. Trumbly Suppression of sdh? Mutations by the SDH1b Gene of Saccharomyces cerevisiae G. Colby, Y. Ishii and A. Tzagoloff 1001 Studies of Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Effect of Inhibitors and Low Temperature L. M. Ducrey Santopietro and M.-R. Kula AILV1 Gene from the Yeast Arxu/a adeninivorans LS3—a New Selective Transformation Marker T. Wartmann, H. Rosel, |. Kunze, R. Bode and G. Kunze YEAST FUNCTIONAL ANALYSIS REPORTS Functional Analysis of Three Adjacent Open Reading Frames from the Right Arm of Yeast Chromosome XVI B. WaSkiewicz-Staniorowska, J. Skata, M. Jasinski, M. Grenson, A. Goffeau and S. Utaszewski A Search in the Genome of Saccharomyces cerevisiae for Genes Regulated via Stress Response Elements E. Moskvina, C. Schuller, C. T. C. Maurer, W. H. Mager and H. Ruis YEAST SEQUENCING REPORTS The Uracil Permease of Schizosaccharomyces pombe: A Representative of a Family of 10 Transmembrane Helix Transporter Proteins of Yeasts J. de Montigny, M. L. Straub, R. Wagner, M. L. Bach and M. R. Chevallier CURRENT AWARENESS ON YEAST ISSUE No. 12, 15 SEPTEMBER 1998 REVIEW Candida rugosa Lipases: Molecular Biology and Versatility in Biotechnology S. Benjamin and A. Pandey Transient mRNA Responses in Chemostat Cultures as a Method of Defining Putative Regulatory Elements: Application to Genes Involved in Saccharomyces cerevisiae Acety|-coenzyme A Metabolism M. A. van den Berg, P. de Jong-Gubbels and H. Y. Steensma A Highly Conserved Intraspecies Homolog of the Saccharomyces cerevisiae Elongation Factor-3 Encoded by the HEF3 Gene T. C. Maurice, C. E. Mazzucco, C. S. Ramanathan, B. M. Ryan, G. A. Warr and J. W. Puziss Secretion and pH-Dependent Self-Processing of the Pro-Form of the Yarrowi/a lipolytica Acid Extra- cellular Protease R. K. McEwan and T. W. Young YEAST FUNCTIONAL ANALYSIS REPORTS Functional Analysis of Yeast Essential Genes Using a Promoter-Substitution Cassette and the Tetracycline-regulatable Dual Expression System G. Belli, E. Gari, M. Aldea and E. Herrero New Constructs and Strategies for Efficient PCR-based Gene Manipulations in Yeast O. Puig, B. Rutz, B. G. Mattias Luukkonen, S. Kandels-Lewis, E. Bragado-Nilsson and B. Séraphin YEAST SEQUENCING REPORTS Cloning and Sequence of a 3:835 KBP DNA Fragment Containing the H/S4 Gene and a Fragment of a PEX5-Like Gene From Candida albicans F. Navarro-Garcia, R. Pérez-Diaz, A. |. Negredo, J. Pla and C. Nombela ISSUE No. 13, 30 SEPTEMBER 1998 A Spheroplast Rate Assay for Determination of Cell Wall Integrity in Yeast R. Ovalle, S. T. Lim, B. Holder, C. K. Jue, C.W ood Moore and P. N. Lipke Characterization of a Second Gene (ZSOD22) of Na* H* Antiporter from Salt-tolerant Yeast Zygo- saccharomyces rouxii and Functional Expression of ZSOD2 and ZSOD22 in Saccharomyces cerevisiae T. lwaki, Y. Higashida, H. Tsuji, Y. Tamai and Y. Watanabe Regulation of Peroxisomal Proteins and Organelle Proliferation by Multiple Carbon Sources in the Methylotrophic Yeast, Candida bo/dinii Y. Sakai, H. Yurimoto, H. Matsuo and N. Kato Fluorescent Probing of Membrane Potential in Walled Cells: diS-C, (3) Assay in Saccharomyces cerevisiae D. GaSkova, B. Brodska, P. Herman, J. Veée7, J. Malinsky, K. Sigler, O. Benada andJ . PlaSek YEAST FUNCTIONAL ANALYSIS REPORTS Disruption of Six Saccharomyces cerevisiae Genes from Chromosome IV and Basic Phenotypic Analysis of Deletion Mutants M. Carmen Lopez, M. Sanchez, E. Ferminan and A. Dominguez Transcriptional Profiling on all Open Reading Frames of Saccharomyces cerevisiae N. C. Hauser, M. Vingron, M. Scheideler, B. Krems, K. Hellmuth, K.-D. Entian and J. D. Hoheisel YEAST SEQUENCING REPORTS Cloning and Sequence Analysis of the Gene Encoding Invertase (/NV7) from the Yeast Candida utilis F. P. Chavez. T. Pons, J. M. Delgado and L. Rodriguez Cloning and Characterization of the Hansenula polymorpha Homologue of the Saccharomyces cerevisiae PMRI1 Gene H. A. Kang, J.-Y. Kim, S.-M. Ko, C. S. Park, D. D. Y. Ryu, J.-H. Sohn, E.-S. Choi and S.-K. Rhee CURRENT AWARENESS ON YEAST ISSUE No. 14 OCTOBER 1998 Stabilization of Two Families of Critical Targets for Hyperthermic Cell Killing and Acquired Thermo- tolerance of Yeast Cells K. Obuchi, H. lwahashi, J. R. Lepock and Y. Komatsu Cloning of the Candida a!bicans Nucleoside Transporter by Complementation of Nucleoside Transport- deficient Saccharomyces S. Detke Comparison of Expression Systems in the Yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of Two Novel Promoters from Yarrowia lipolytica S. Muller, T. Sandal, P. Kamp-Hansen and H. Dalboge Characterization of Deletion Mutations in the Carboxy-terminal Peptide-binding Domain of the Kar2 Protein in Saccharomyces cerevisiae M. Tokunaga, S. Kato, A. Kawamura-Watabe, R. Tanaka and H. Tokunaga YEAST FUNCTIONAL ANALYSIS REPORTS A New Method for Quantitative Determination of Polysaccharides in the Yeast Cell Wall. Application to the Cell Wall Defective Mutants of Saccharomyces cerevisiae N. Dallies, J. M. Francois and V. Paquet YEAST SEQUENCING REPORTS Molecular Cloning and Sequence Analysis of the Schizosaccharomyces pombe ade10° Gene C. Liedtke and H. Schmidt Molecular Cloning of Alcohol Dehydrogenase Genes of the Yeast Pichia stipitis and |Identification of the Fermentative ADH Passoth, B. Schafer, B. Liebel, T. Weierstall and U. Klinner Single-read Sequence Tags of a Limited Number of Genomic DNA Fragments Provide an Inexpensive Tool for Comparative Genome Analysis K. Hartung, D. Frishman, A. Hinnen and S. Wolfi CURRENT AWARENESS ON YEAST ISSUE No. 15 NOVEMBER 1998 inter- and Intraspecific Chromosome Pattern Variation in the Yeast Genus K/uyveromyces C. Belloch, E. Barrio, M. D. Garcia and A. Querol Monovalent Cation Fluxes and Physiological Changes of Debaryomyces hansenii Grown at High Concen- trations of KCI and NaCl P. E. Thomé-Ortiz, A. Peha and J. Ramirez Cloning and Characterization of the Peroxisomal Acyl CoA Oxidase ACO3 Gene from the Alkane-utilizing Yeast Yarrowia lipolytica H. Wang, A. Le Clainche, M.-T. Le Dall, Y. Wache, Y. Pagot, J.-M. Belin, C. Gaillardin and J.-M. Nicaud Cloning and Characterization of an n-alkane-inducible Cytochrome P450 Gene Essential for n-Decane Assimilation by Yarrowi/a lipolytica T. lida, A. Ohta and M. Takagi YEAST SEQUENCING REPORTS Isolation and Sequence Analysis of the Orotidine-5'-phosphate Decarboxylase Gene (URA3) of Candida utilis. Comparison with the OMP Decarboxylase Gene Family L. Rodriguez, F. P. Chavez, M. E. Gonzalez, L. Basabe and T. Rivero 1399 YEAST FUNCTIONAL ANALYSIS REPORTS Vectors for Transcription Factor Cloning and Target Site Identification by Means of Genetic Selection in Yeast A. H. Meyer, P. B. F. Quwerkerk and J. H. C. Hoge Quantitative Analysis of Yeast Gene Function by Using Competition Experiments in Continuous Culture F. Baganz, A. Hayes, R. Farquhar, P. R. Butler, D. C. J. Gardner and S. G. Oliver CURRENT AWARENESS ON YEAST ISSUE No. 16, YEAST 1998, DECEMBER 1998 Publisher's Note List of Referees EDITORIAL This Year in YEAST R. B. Wickner REVIEWS A History of Research on Yeasts 1: Work by Chemists and Biologists 1789-1850 J. A. Barnett 1439 Expanding Yeast Knowledge Online K. Dolinski, C. A. Ball, S. A. Chervitz, S. S. Dwight, M. A. Harris, S. Roberts, T. Y. Roe, J. M. Cherry and D. Botstein 1453 Biochemistry, Cell Biology and Molecular Biology of Lipids of Saccharomyces cerevisiae G. Daum, N. D. Lees, M. Bard and R. Dickson 1471 Oxidative Stress Responses of the Yeast Saccharomyces cerevisiae D. J. Jamieson 1511 Fusion of a Fission Yeast J. Davey 1529 Author Index 1567 Keyword Index 1571 Volume Contents