Working with Stem Cells PDF
Preview Working with Stem Cells
Henning Ulrich · Priscilla Davidson Negraes Working with Stem Cells Working with Stem Cells Henning Ulrich • Priscilla Davidson Negraes Working with Stem Cells A Quick and Easy Approach of Methodologies and Applications Henning Ulrich Priscilla Davidson Negraes Department of Biochemistry Department of Biochemistry Institute of Chemistry Institute of Chemistry University of Sao Paulo University of São Paulo São Paulo , SP , Brazil São Paulo , SP , Brazil ISBN 978-3-319-30580-6 ISBN 978-3-319-30582-0 (eBook) DOI 10.1007/978-3-319-30582-0 Library of Congress Control Number: 2016945374 © Springer International Publishing Switzerland 2016 T his work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. T he publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer International Publishing AG Switzerland Pref ace We are in a unique moment in human history where stem cells, previously viewed as a great promise in medicine, are now slowly moving into clinical trials. Scientists are now confi dent to start testing different stem cell-based protocols in humans. For the fi rst time, we will be the pioneers, having the opportunity to do it right, setting the example for the generations to come. Contrary to conventional medicine, cel- lular therapies will replace damaged tissues and stay there for the lifespan of a per- son. Alternatively, stem cells can be used as a tool to recapitulate human development and inform us about the disease pathology, proving opportunities for novel diagnos- tics and therapies. This is an extraordinary example of plasticity, never seen before in medical history. The fundamental aspects of stem cells come back to the methods that were developed over the years to reach to this point. We have learnt how to isolate, grow, enrich, and differentiate stem cells. These methods are still evolving in the scientifi c laboratories all over the world and will be optimized for the differ- ent medical strategies. This book summarizes the most current and important meth- ods, and state-of-the art protocols to manipulate stem cells. It should not be seen as a fi xed textbook, but a snapshot of a new and dynamic era in medicine. San Diego, CA, USA Alysson Renato Muotri, Ph.D. v Contents 1 Stem Cells: Principles and Applications ............................................... 1 Ágatha Oliveira , Juliana da Cruz Corrêa-Velloso , Talita Glaser , and Henning Ulrich 2 Human Embryonic Stem Cell Line Derivation .................................... 15 Simone Aparecida Siqueira Fonseca , Roberta Montero Costas , and Lygia V. Pereira 3 Adipose-Derived Mesenchymal Stromal Cells ..................................... 37 Amanda Faria Assoni , Giuliana Castello Coatti , Juliana Plat Aguiar Gomes , Mayra Vitor Pelatti , and Mayana Zatz 4 Neural Differentiation of Rodent Neural Progenitor Cells and Isolation and Enrichment of Human Neural Progenitor/Stem Cells ............................................................................. 57 Antonio H. Martins , Jose L. Roig-Lopez , and Maxine Nicole Gonzalez 5 Mice Post-natal Subventricular Zone Neurospheres: Derivation, Culture, Differentiation and Applications ........................ 79 Laura Sardá-Arroyo , Clarissa Schitine , Sara Alves Xapelli , and Henning Ulrich 6 Very Small Embryonic Like Stem Cells (VSELs) and Their Hematopoietic Specification ................................................. 97 Malwina Suszynska , Mariusz Z. Ratajczak , and Janina Ratajczak 7 Neural Crest Stem Cell Cultures: Establishment, Characterization and Potential Use....................................................... 111 Andréa Gonçalves Trentin , Ricardo Castilho Garcez , and Raul Bardini Bressan vii viii Contents 8 Cancer Stem Cells: Issues with In Vitro Expansion and Model Systems ................................................................................. 127 Khadidiatou Guiro , Garima Sinha , Oleta Sandiford , Treena L. Arinzeh , and Pranela Rameshwar 9 Spontaneous Generation of Patient-Specific Retinal Pigment Epithelial Cells Using Induced Pluripotent Stem Cell Technology .............................................................................. 143 David A. Carter , Britta Nommiste , Pete J. Coffey , and Amanda-Jayne F. Carr 10 Differentiation of Human Pluripotent Stem Cells into Cortical Neurons.............................................................................. 163 Cassiano Carromeu , Alexandre Vessoni , Ana Paula Diniz Mendes , and Patricia Cristina Baleeiro Beltrão-Braga 11 Motor Neuron Differentiation from Pluripotent Stem Cells: Development of the Technique, Synopsis of Protocols, and Summary of Current Applications ................................................ 181 Helen Cristina Miranda and Albert R. La Spada 12 Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or Small Molecules .............. 203 Atossa Shaltouki 13 Differentiation of hiPSC-Derived Cardiomyocytes .............................. 219 Fabian Zanella and Farah Sheikh 14 Endoderm Differentiation from Human Pluripotent Stem Cells ....... 237 Nathan Kumar , David Brafman , and Karl Willert 15 Pancreatic Differentiation from Human Pluripotent Stem Cells ....... 257 Nicholas Vinckier , Jinzhao Wang , and Maike Sander 16 Efficient Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells ..................................................... 277 Jaemin Kim , Alessandro Magli , and Rita C. R. Perlingeiro 17 Genome Editing in Stem Cells ............................................................... 287 Leon Tejwani , Cleber A. Trujillo , Charles A. Thomas , and Alysson R. Muotri 18 Neural Stem Cells: Functional Multipotency and Spinal Cord Injury Research Protocols ......................................... 311 Yang D. Teng , Xiang Zeng , Inbo Han , and Jaime E. Anderson Contents ix 19 Stem Cells and Tissue Engineering ....................................................... 331 Fernanda Maria Policarpo Tonelli , Nicole de Cássia Oliveira Paiva , Rebecca Vasconcellos Botelho de Medeiros , Mauro Cunha Xavier Pinto , Flávia Cristina Policarpo Tonelli, and Rodrigo Ribeiro Resende 20 Scaffolds for Embryonic Stem Cell Growth and Differentiation ........ 347 Ana Teresa Semeano , Talita Glaser , Henning Ulrich , and Denise Freitas Siqueira Petri Index ................................................................................................................. 367
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