Wheat biotechnology : methods and protocols

309 Pages·2017·7.553 MB·English

by  Bhalla, Prem L., Singh, Mohan B

Methods in
Molecular Biology 1679
Prem L. Bhalla
Mohan B. Singh
Editors
Wheat
Biotechnology
Methods and Protocols
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire,AL109AB,UK
Forfurther volumes:
http://www.springer.com/series/7651
Wheat Biotechnology
Methods and Protocols
Edited by
Prem L. Bhalla and Mohan B. Singh
Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food,
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Parkville, VIC, Australia
Editors
PremL.Bhalla MohanB.Singh
PlantMolecularBiologyandBiotechnology PlantMolecularBiologyandBiotechnology
Laboratory,SchoolofAgriculture Laboratory,SchoolofAgriculture
andFood,FacultyofVeterinary andFood,FacultyofVeterinary
andAgriculturalSciences andAgriculturalSciences
TheUniversityofMelbourne TheUniversityofMelbourne
Parkville,VIC,Australia Parkville,VIC,Australia
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-4939-7335-4 ISBN978-1-4939-7337-8 (eBook)
DOI10.1007/978-1-4939-7337-8
LibraryofCongressControlNumber:2017951629
©SpringerScience+BusinessMediaLLC2017
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Preface
Wheat has long been integral to human civilization. Humans have consumed wheat for
thousands of years. The present-day bread wheat is a polyploid crop consisting of three
genomes believed to have originated around 10,000 BC. Wheat is one of the major staple
foodcropsgrownglobally;itprovidesasignificantpart(around50%)ofcaloriesforhumans.
Wheatgrainsarerichincarbohydrates,proteins,essentialvitamins,calcium,iron,fiber,and
othermicronutrientsimportantforgeneralgoodhealth.Moreover,thewheatgrainprotein,
gluten, is also used in the pharmaceutical and paper-making industries. The carbohydrate-
richvegetativepartsofawheatplantcanalsobeusedforbio-fuelproduction.China,India,
and the USA are currently the leading wheat-producing countries, followed by the former
USSR,France,andCanada.
The green revolution that resulted from high-yielding dwarf wheat and rice varieties
turnedcountrieslikeIndiafromthebrinkofmassfaminetoanetexporterofwheat.Wheat
istheprimarycropgrownglobally,occupyingthedominantpositioninworldgraintrade.
Our society is facing multidimensional challenges from climate change and population
growth.Tomeetourfuturedemands,weneedtogrowmorefoodonthesameareaofarable
land;however,thelandavailableforcultivationisdecreasingduetourbanizationandother
nonagricultural uses. Moreover, food production and distribution are severely affected by
unforeseenclimaticconditions.Limitedwater forcropirrigationandhighenergycostsput
anextrastrainonfoodproduction.
Globalwheatproductionhasbeendeclining,thusraisingconcernsaboutfoodsecurity.
Worldwide wheat production in 2017 has been forecasted to reach 744.5 million tons by
FAO,indicatingareductionfrom2016.Moreover,thereisavariationinwheatproduction
fromyear toyear,whichaddsuncertainty.Therefore,amajorgoalistodevelopknowledge
and technology in order to breed wheat varieties that can show yield stability in varying
environmentalconditions.
Conventionalplantbreedingislimitedbytheavailablegenepool,whichcannotsustain
the growth in wheat production and meet the growing demand. Recent progress in
molecular biology, including recombinant methods, has the potential to breed future
high-yielding wheat varieties that require less fertilizer and herbicides and are disease and
stresstolerant.
The aim of Wheat Biotechnology: Methods and Protocols is to provide a background on
recentnewtechnologydevelopmentshighlightingthepotentialofthegenomicerainwheat
breeding with detailed instruction on the methodology, which is complemented by over-
view chapters on the status of new technology application in major wheat producing
countries. The protocol chapters contain an introduction to bring the reader up to speed
on the literature, followed by detailed step-by-step instructions for applying these techni-
quesandmethods,andnotesontroubleshooting.Thetopicscovermethodsunderpinning
the latest developments in the field of wheat biotechnology. The chapters are written by
internationallyrenownedscientistswhoareactivelyworkingoncutting-edgetechnologyfor
v
vi Preface
wheat. This book is directed at individuals in academia and industry alike and forms a
valuable resource for educators and beginners in the area of applying biotechnological
techniquesinwheatbreeding.
Parkville,VIC,Australia PremL.Bhalla
MohanB.Singh
Contents
Preface ..................................................................... v
Contributors................................................................. ix
PART I BACKGROUND AND OVERVIEW
1 EnablingMolecularTechnologiesforTraitImprovementinWheat ........... 3
PremL.Bhalla,AkankshaSharma,andMohanB.Singh
2 WhatWillBetheBenefitsofBiotechWheatforEuropeanAgriculture?........ 25
Agne`sE.Ricroch
3 OverviewoftheWheatGeneticTransformationandBreeding
StatusinChina ......................................................... 37
JiapengHan,XiaofenYu,JunliChang,GuangxiaoYang,
andGuangyuanHe
4 WheatImprovementinIndia:PresentandFuture........................... 61
SoniaGoel,KalpanaSingh,andN.K.Singh
5 OverviewofMethodsforAssessingSalinityandDrought
ToleranceofTransgenicWheatLines...................................... 83
RohitJoshi,KhalidAnwar,PriyankaDas,SnehL.Singla-Pareek,
andAshwaniPareek
6 AllergenicityAssessmentofTransgenicWheatLinesInSilico................. 97
AnkitaMishraandNaveenArora
7 AgribusinessPerspectivesonTransgenicWheat............................. 113
BillMalcolm
PART II PROTOCOLS
8 Agrobacterium-MediatedTransformationofWheatUsing
ImmatureEmbryos ..................................................... 129
GoetzHensel,CorneliaMarthe,andJochenKumlehn
9 BiolisticTransformationofWheat......................................... 141
CarolineTassyandPierreBarret
10 WheatGeneticTransformationUsingMatureEmbryosasExplants........... 153
HarshChauhanandParamjitKhurana
11 TargetedMutagenesisinHexaploidBreadWheatUsing
theTALENandCRISPR/CasSystems.................................... 169
YanpengWang,YuanZong,andCaixiaGao
12 DesignandAssemblyofCRISPR/Cas9ReagentsforGene
Knockout,TargetedInsertion,andReplacementinWheat................... 187
Toma´ˇsCˇerma´kandShaunJ.Curtin
13 DoubledHaploidTransgenicWheatLinesbyMicrosporeTransformation..... 213
SachinRustgi,NiiO.Ankrah,RhodaA.T.Brew-Appiah,
YueSun,WeiguoLiu,andDiter vonWettstein
vii
viii Contents
14 DoubledHaploidLaboratoryProtocolforWheatUsing
Wheat–MaizeWideHybridization ........................................ 235
MeenakshiSantra,HongWang,ScottSeifert,andScottHaley
15 Real-TimePCRfor theDetectionofPreciseTransgeneCopy
NumberinWheat....................................................... 251
AngelicaGiancaspro,AgataGadaleta,andAntonioBlanco
16 EndogenousReferenceGenesandTheirQuantitative
Real-TimePCRAssaysforGeneticallyModifiedBreadWheat
(TriticumaestivumL.)Detection......................................... 259
LitaoYang,ShengQuan,andDabingZhang
17 PhenotypicCharacterizationofTransgenicWheatLinesAgainst
FungalPathogensPucciniatriticinaandFusariumgraminearum ............ 269
JagdeepKaur,DilipShah,andJohnFellers
18 DatabasesforWheatGenomicsandCropImprovement..................... 277
YuxuanYuan,ArminScheben,Chon-KitKennethChan,
andDavidEdwards
19 High-DensitySNPGenotypingArrayforHexaploidWheat
andItsRelatives ........................................................ 293
AmandaJ.Burridge,MarkO.Winfield,AlexandraM.Allen,
PaulA.Wilkinson,GaryL.A.Barker,JaneCoghill,
ChristyWaterfall,andKeithJ.Edwards
Index ...................................................................... 307
Contributors
ALEXANDRAM.ALLEN (cid:1) SchoolofBiologicalSciences,UniversityofBristol,Bristol,UK
NIIO.ANKRAH (cid:1) DepartmentofCropandSoilSciences,WashingtonStateUniversity,
Pullman,WA,USA;SchoolofBiologicalSciences,WashingtonStateUniversity,Pullman,
WA,USA
KHALIDANWAR (cid:1) StressPhysiologyandMolecularBiologyLaboratory,SchoolofLifeSciences,
JawaharlalNehruUniversity,NewDelhi,Delhi,India
NAVEENARORA (cid:1) AllergyandImmunologySection,CSIR—InstituteofGenomicsand
IntegrativeBiology,Delhi,India
GARYL.A.BARKER (cid:1) SchoolofBiologicalSciences,UniversityofBristol,Bristol,UK
PIERREBARRET (cid:1) INRA,UMRGDEC,Genetic,DiversityandEcophysiologyofCereals,
ClermontFerrand,France
PREML.BHALLA (cid:1) PlantMolecularBiologyandBiotechnologyLaboratory,Schoolof
AgricultureandFood,FacultyofVeterinaryandAgriculturalSciences,TheUniversity
ofMelbourne,Parkville,VIC,Australia
ANTONIO BLANCO (cid:1) DepartmentofSoil,PlantandFoodSciences,UniversityofBari
“AldoMoro”,Bari,Italy
RHODAA.T.BREW-APPIAH (cid:1) DepartmentofCropandSoilSciences,WashingtonState
University,Pullman,WA,USA
AMANDAJ.BURRIDGE (cid:1) SchoolofBiologicalSciences,UniversityofBristol,Bristol,UK
TOMA´Sˇ CˇERMA´K (cid:1) DepartmentofGenetics,CellBiologyandDevelopmentandCenter
forGenomeEngineering,UniversityofMinnesota,Minneapolis,MN,USA
CHON-KITKENNETHCHAN (cid:1) SchoolofPlantBiologyandInstituteofAgriculture,
UniversityofWesternAustralia,Crawley,WA,Australia
JUNLICHANG (cid:1) TheGeneticEngineeringInternationalCooperationBaseofChinese
MinistryofScienceandTechnology,KeyLaboratoryofMolecularBiophysicsofChinese
MinistryofEducation,CollegeofLifeScienceandTechnology,HuazhongUniversityof
ScienceandTechnology,Wuhan,China
HARSHCHAUHAN (cid:1) IndianInstituteofTechnologyRoorkee,Roorkee,India
JANECOGHILL (cid:1) SchoolofBiologicalSciences,UniversityofBristol,Bristol,UK
SHAUN J.CURTIN (cid:1) CerealDiseaseLaboratory,AgriculturalResearchService,United
StatesDepartmentofAgriculture,StPaul,MN,USA
PRIYANKA DAS (cid:1) StressPhysiologyandMolecularBiologyLaboratory,SchoolofLifeSciences,
JawaharlalNehruUniversity,NewDelhi,Delhi,India
DAVIDEDWARDS (cid:1) SchoolofPlantBiologyandInstituteofAgriculture,Universityof
WesternAustralia,Crawley,WA,Australia
KEITHJ.EDWARDS (cid:1) SchoolofBiologicalSciences,UniversityofBristol,Bristol,UK
JOHNFELLERS (cid:1) DepartmentofPlantPathology,USDA-ARS-HWWGRU,Manhattan,KS,
USA
AGATAGADALETA (cid:1) DepartmentofEnvironmentalandTerritorialSciences,Universityof
Bari“AldoMoro”,Bari,Italy
CAIXIAGAO (cid:1) StateKeyLaboratoryofPlantCellandChromosomeEngineering,and
Center forGenomeEditing,InstituteofGeneticsandDevelopmentalBiology,Chinese
AcademyofSciences,Beijing,China
ix
x Contributors
ANGELICAGIANCASPRO (cid:1) DepartmentofEnvironmentalandTerritorialSciences,
UniversityofBari“AldoMoro”,Bari,Italy
SONIAGOEL (cid:1) NationalResearchCentreonPlantBiotechnology,IndianCouncilof
AgriculturalResearch,NewDelhi,India
SCOTTHALEY (cid:1) DepartmentofSoilandCropScience,ColoradoStateUniversity,FortCollins,
CO,USA
JIAPENGHAN (cid:1) TheGeneticEngineeringInternationalCooperationBaseofChinese
MinistryofScienceandTechnology,KeyLaboratoryofMolecularBiophysicsofChinese
MinistryofEducation,CollegeofLifeScienceandTechnology,HuazhongUniversity
ofScienceandTechnology,Wuhan,China
GUANGYUANHE (cid:1) TheGeneticEngineeringInternationalCooperationBaseofChinese
MinistryofScienceandTechnology,KeyLaboratoryofMolecularBiophysicsofChinese
MinistryofEducation,CollegeofLifeScienceandTechnology,HuazhongUniversity
ofScienceandTechnology,Wuhan,China
GOETZ HENSEL (cid:1) PlantReproductiveBiology,LeibnizInstituteofPlantGeneticsand
CropPlantResearch(IPK),Seeland/OTGatersleben,Germany
ROHITJOSHI (cid:1) StressPhysiologyandMolecularBiologyLaboratory,SchoolofLifeSciences,
JawaharlalNehruUniversity,NewDelhi,Delhi,India
JAGDEEP KAUR (cid:1) DonaldDanforthPlantScienceCenter,SaintLouis,MO,USA
PARAMJITKHURANA (cid:1) DepartmentofPlantMolecularBiology,UniversityofDelhiSouth
Campus,NewDelhi,India
JOCHEN KUMLEHN (cid:1) PlantReproductiveBiology,LeibnizInstituteofPlantGeneticsand
CropPlantResearch(IPK),Seeland/OTGatersleben,Germany
WEIGUOLIU (cid:1) Syngenta,ResearchTrianglePark,Cary,NC,USA
BILLMALCOLM (cid:1) SchoolofAgricultureandFood,FacultyofVeterinaryandAgricultural
Sciences,TheUniversityofMelbourne,Parkville,VIC,Australia
CORNELIAMARTHE (cid:1) PlantReproductiveBiology,LeibnizInstituteofPlantGeneticsand
CropPlantResearch(IPK),Seeland/OTGatersleben,Germany
ANKITAMISHRA (cid:1) AllergyandImmunologySection,CSIR—InstituteofGenomicsand
IntegrativeBiology,Delhi,India
ASHWANIPAREEK (cid:1) StressPhysiologyandMolecularBiologyLaboratory,SchoolofLifeSciences,
JawaharlalNehruUniversity,NewDelhi,Delhi,India
SHENG QUAN (cid:1) NationalCenter forMolecularCharacterizationofGeneticallyModified
Organisms,SJTU-BorLuhFoodSafetyCenter,SchoolofLifeScienceandBiotechnology,
ShanghaiJiaoTongUniversity,Shanghai,China
AGNE`SE.RICROCH (cid:1) EvolutionaryGeneticsandPlantBreeding,AgroParisTech,Paris,
Cedex05,France;Durability,Innovation,ResourcesandEthics,UniversityParis-Sud,
Paris,France;CollegeofInterdisciplinaryStudies,Universite´ Paris-Saclay,Paris,France
SACHINRUSTGI (cid:1) DepartmentofPlantandEnvironmentalSciences,ClemsonUniversity
PeeDeeResearchandEducationCenter,Florence,SC,USA;DepartmentofCropand
SoilSciences,WashingtonStateUniversity,Pullman,WA,USA
MEENAKSHISANTRA (cid:1) DepartmentofSoilandCropScience,ColoradoStateUniversity,
FortCollins,CO,USA
ARMIN SCHEBEN (cid:1) SchoolofPlantBiologyandInstituteofAgriculture,Universityof
WesternAustralia,Crawley,WA,Australia
SCOTTSEIFERT (cid:1) DepartmentofSoilandCropScience,ColoradoStateUniversity,
FortCollins,CO,USA
DILIPSHAH (cid:1) DonaldDanforthPlantScienceCenter,SaintLouis,MO,USA