VARIETAL CHARACTERIZATION AND ABIOTIC STRESS STUDIES ON SEED QUALITY OF ...

172 Pages·2015·4.8 MB·English

VARIETAL CHARACTERIZATION AND ABIOTIC
STRESS STUDIES ON SEED QUALITY OF
BRINJAL (Solanum melongena L.) VARIETIES

RAJATHA, K. D.
PALB 3279

DEPARTMENT OF SEED SCIENCE AND TECHNOLOGY
UNIVERSITY OF AGRICULTURAL SCIENCES
BENGALURU
2015
VARIETAL CHARACTERIZATION AND ABIOTIC
STRESS STUDIES ON SEED QUALITY OF
BRINJAL (Solanum melongena L.) VARIETIES

RAJATHA, K. D.
PALB 3279

Thesis submitted to the
UNIVERSITY OF AGRICULTURAL SCIENCES, BENGALURU
In partial fulfillment of the requirements
For the award of the Degree of
Master of Science (Agriculture)
In
Seed Science and Technology
BENGALURU JULY, 2015
Affectionately Dedicated to

My Beloved Parents

Sri Dharmappa K.S.

Smt. Kalpana G.C.

My sweet sister

Harshitha K.D.

and to my teacher

Dr. B.C Channakeshava
DEPARTMENT OF SEED SCIENCE AND TECHNOLOGY
UNIVERSITY OF AGRICULTURAL SCIENCES
BENGALURU 560 065
CERTIFICATE
This is to certify that the thesis entitled “Varietal characterization and abiotic
stress studies on seed quality of Brinjal (Solanum melongena L.) varieties” submitted
by Ms. RAJATHA K.D., I.D NO. PALB 3279, in partial fulfilment of the requirements
for the award of the degree of MASTER OF SCIENCE (AGRICULTURE) in SEED
SCIENCE AND TECHNOLOGY of the University of Agricultural Sciences,
BENGALURU, is a record of research work done by her during the period of her study in
this University under my guidance and supervision and the thesis has not previously
formed the basis for the award of any degree, diploma, associateship, fellowship or other
similar titles.

BENGALURU (B.C. CHANNAKESHAVA)
July, 2015 Major Advisor
Professor and Head
Approved by:
Chairman: ___________________________
(B.C. CHANNAKESHAVA)
Members: 1. ___________________________
(K. BHANUPRAKSH)
2. __________________________
(T.B. PUTTARAJU)
3. ___________________________
(VISWANATH KOTI)
ACKNOWLEDGEMENT
With great memories …!!!
The moment has come to look into the deeper layer of heart, which is filled with
the feelings of togetherness, loveliness, consolation and satisfaction, a sign of relief and
sense of fulfilment. Some are momentary and some are permanent, but both involve a
number of near and dear persons to whom I acknowledge my warm regards and take this
opportunity to express my feelings during the course of my research and it is putting
down in black and white.
I place on record my deep sense of gratitude and heartfelt respects to
Dr. B.C. CHANNAKESHAVA, Professor and Former Head, Department of Seed
Science & Technology, University of Agricultural Sciences, Bengaluru, Chairman of my
advisory committee, for being very motivating force during the course of investigation.
His valuable guidance, keen interest helped me to a great extent in the preparation of
thesis. It was my great pleasure to be associated with him and I find no words to express
my thanks for all kindness shown by him in completion of this manuscript without which
the work of mine is partial.
I express my gratitude to Dr. K. BHANUPRAKASH, Principle scientist, Division
of Seed Science and Technology, IIHR, Hesaraghatta, Bengaluru, for being member of
my advisory committee with providing all the facilities timely as and when required for
which I am greatly indebted to him.
I thank profusely to Dr. T.B. PUTTARAJU, Professor of Hort. and Technical
officer College of Agriculture,UAS, GKVK, Benagluru, being member of my advisory
committee for his encouragement, valuable suggestions and kindness.
I thank my deepest gratitude to to Dr. VISHWANATH KOTI, Assistant
professor,ZARS, VC Farm, Mandya, being member of my advisory committee for his kind
support and valuable intimation in providing for completion of this manuscript.
I thank profusely, Dr. BALAKRISHNA, Professor and Head Department of Seed
Science & Technology, University of Agricultural Sciences, Mr. R. PARAMESH,
Associate Professor, Department of Seed Science & Technology, University of
Agricultural Sciences, Bengaluru, for his encouragement, valuable suggestions and
kindness.
This thesis would be incomplete if I do not reckon the sacrifices, love, affection
and support of my father Sri. DHARMAPPA K.S., my mother Smt. KALPANA G.C my
sister HARSHITHA K.D., my father in law SOMSHEKAR and my lovely grandmother
KAMALAMMA without whose love and affection this task would not have been possible.
I would like to thank my friends, Sinchu, Deepak ola, Nikhil, Neha, Usha, Vishu,
Meghana, Chandu, Roopa O.M, Shobha D.M, Lloyd, Smitha,, Moksha, Arpitha,
Brijesh, Jayanth, Naneshwar, Sumalatha, Vidhya, Inchara and my relatives Sudha,
Divya, Shobha, Anitha Devi, Prajwala, Dimpi, Suppi, Supriya D, Lohith, Pari, Pallavi
and Vimala for their kind co-operation and timely help throughout the study, for their
moral support, and love which always help me to stand stron.
I strongly beholden to all my beloved seniors Nagaraj Hullur, Radha, B. N.,
Chaithra, Maithreyee, Sarika, Amrutha, Latha, Deepika, Ranjitha, Nagraju, Umesh,
Gangaraju, Manjunath, Hareesh and Siddaruda for their support and help throughout
my course of study and preparation of thesis. I am ever grateful to my dearest friends
Shruthi K, Dileep, Balappa, , Vittal Chabbi, Salman, Sarang for constructive censure
and encouragement right through my course of study and completion of this manuscript
eventually.
I owe my heartfelt gratitude to my juniors Shuba, Sahana and all for their
encouragement and the things they do in pleasant friendly ways to me.
I express my thanks to staff of Seed Science and Technology Mr. Venugopalraj
Urs, Mr. Udhay Mrs. Pornima and Mr Manju staff of IIHR for their help and
cooperation during my research work.
No less share of credit goes to all other galaxies and oceans of friends for their
timely help and good company during the course of study and completion of this
manuscript. In the light of reaching a milestone in my life, I owe my deep sense of
gratitude to all those who helped me in a constructive fashion.

Bengaluru
July, 2015 [RAJATHA, K. D.]
Varietal characterization and abiotic stress studies in seed quality
of Brinjal (Solanum melongena L.) varieties
Rajatha, K. D.
Abstract
Brinjal is one of the most important vegetable crop in India. Present investigation
was undertaken to characterize 12 brinjal genotypes based on the morphological traits
and to study the effect of drought and high temperature stress on seed quality parameters.
Drought and high temperature stress were imposed on four brinjal genotypes to study the
seed quality parameters by using three concentrations of PEG 6000 (0, -1 MPa and -1.5
MPa) and subjecting seeds to three different temperature (25 °C, 35 °C and 40 °C).
Characterization studies revealed wide variation among genotypes for most of the
parameters studied viz., seed size, anthocyanine pigmentation, vein colour, fruit
shape, flower size and flower colour. Brinjal green round was the sole genotype which
exhibited small sized flowers, whereas Arka Shirish and Arka Kusumakar showed
greenish white colour flower. The experimental results revealed that drought stress and
high temperature resulted in decreased seed germination, mean seedling length, seedling
vigour index, total dehydrogenase activity and increased electrical conductivity. Drought
stress (-1.5 MPa) reduced the germination per cent by (9 %) when compared to control
(90 %). Similarly in high temperature stress (40 °C) the germination per cent in all the
genotypes is zero, whereas in control recorded 88.5 %. As the stress increases there was
significant increase in the activity of peroxidase (POD) and esterase (EST) under both
high temperature and drought stress, irrespective of genotypes. However among the four
genotypes studied in the experiment, Arka Anand maintained the highest seed quality
under both the high temperature and drought stress.

July, 2015 (B. C. Channakeshava)
Department of Seed Science and Technology Major Advisor
UAS, G. K. V. K., Bengaluru-560065
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CONTENTS
CHAPTER TITLE PAGE No.
I INTRODUCTION 1-3
II REVIEW OF LITERATURE 4-16
III MATERIAL AND METHODS 17-36
IV EXPERIMENTAL RESULTS 37-95
V DISCUSSION 96-109
VI SUMMARY 110-114
VII REFERENCES 115-128
APPENDICES 129-130