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University of Trás-os-Montes and Alto Douro PhD thesis in Veterinary Sciences - Clinic ANA ISABEL ROCHA FAUSTINO Supervisor: Professor Doutor Mário Manuel Dinis Ginja Co-supervisor: Professora Doutora Rita Maria Pinho Ferreira Co-supervisor: Professora Doutora Adelina Maria Gaspar Gama Quaresma Vila Real, 2017 University of Trás-os-Montes and Alto Douro PhD thesis in Veterinary Sciences - Clinic Ana Isabel Rocha Faustino Supervisor: Professor Doutor Mário Manuel Dinis Ginja Co-supervisor: Professora Doutora Rita Maria Pinho Ferreira Co-supervisor: Professora Doutora Adelina Maria Gaspar Gama Quaresma Composition of the jury: ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ Vila Real, 2017 Original thesis presented by Ana Isabel Rocha Faustino at the University of Trás-os- Montes and Alto Douro, to obtain the Doctor’s degree in Veterinary Sciences - Clinic. This work was supported by Fundação para a Ciência e Tecnologia, Ministério da Ciência, Tecnologia e Ensino Superior (Portugal). - SFRH/BD/102099/2014 - To my Parents. vii viii

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protocol of NovoLink Polymer Detection System (Leica Biosystems, Newcastle, UK). Sections were incubated during one and half hour at room temperature with primary antibodies for ER α (clone 6F11, Novocastra, Newcastle, UK) and for ER β (clone. MCA1974S, AbD Serotec, Oxford, UK) at a dilution

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University of Trás-os-Montes and Alto Douro PhD thesis in Veterinary Sciences - Clinic ANA ISABEL ROCHA FAUSTINO Supervisor: Professor Doutor Mário Manuel Dinis Ginja Co-supervisor: Professora Doutora Rita Maria Pinho Ferreira Co-supervisor: Professora Doutora Adelina Maria Gaspar Gama Quaresma Vila Real, 2017 University of Trás-os-Montes and Alto Douro PhD thesis in Veterinary Sciences - Clinic Ana Isabel Rocha Faustino Supervisor: Professor Doutor Mário Manuel Dinis Ginja Co-supervisor: Professora Doutora Rita Maria Pinho Ferreira Co-supervisor: Professora Doutora Adelina Maria Gaspar Gama Quaresma Composition of the jury: ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ Vila Real, 2017 Original thesis presented by Ana Isabel Rocha Faustino at the University of Trás-os- Montes and Alto Douro, to obtain the Doctor’s degree in Veterinary Sciences - Clinic. This work was supported by Fundação para a Ciência e Tecnologia, Ministério da Ciência, Tecnologia e Ensino Superior (Portugal). - SFRH/BD/102099/2014 - To my Parents. vii viii

Description:

protocol of NovoLink Polymer Detection System (Leica Biosystems, Newcastle, UK). Sections were incubated during one and half hour at room temperature with primary antibodies for ER α (clone 6F11, Novocastra, Newcastle, UK) and for ER β (clone. MCA1974S, AbD Serotec, Oxford, UK) at a dilution

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