University of Groningen The development of antimicrobial biomaterial surfaces Gottenbos, Bart

121 Pages·2001·0.98 MB·English

University of Groningen

The development of antimicrobial biomaterial surfaces
Gottenbos, Bart
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2001
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Gottenbos, B. (2001). The development of antimicrobial biomaterial surfaces. s.n.
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Download date: 17-03-2023
The development of antimicrobial
biomaterial surfaces
Copyright © 2001, Gottenbos, Bart
The development of antimicrobial biomaterial surfaces
ISBN 90-367-1447-8
Printed by Ponsen & Looijen
Cover design by Aletta van Rheede, Amsterdam
Publication of this thesis was financially supported by:
Faculty of Medical Sciences
Institute of Biomedical Materials Science and Applications
RIJKSUNIVERSITEIT GRONINGEN
The development of antimicrobial
biomaterial surfaces
PROEFSCHRIFT
ter verkrijging van het doctoraat in de
Medische Wetenschappen
aan de Rijksuniversiteit Groningen
op gezag van de
Rector Magnificus, dr. D.F.J. Bosscher,
in het openbaar te verdedigen op
woensdag 13 juni 2001
om 14.15 uur
door
Bart Gottenbos
geboren op 10 september 1973
te Heesch
Promotores : Prof. Dr. Ir. H.J. Busscher
Prof. Dr. P. Nieuwenhuis
Prof. Dr. J. Feijen
Co-promotor : Dr. H.C. van der Mei
Beoordelingscommissie : Prof. Dr. J. Dankert
Prof. Dr. J.E. Degener
Prof. Dr. A.J. Schouten
ISBN 90-367-1447-8
Paranimfen: Albert Poortinga
Gerda Bruinsma
Voor mama

Contents
Chapter 1 Introduction and aims page 9
Chapter 2 Models for studying initial adhesion and surface growth in page 25
biofilm formation on surfaces
Chapter 3 Initial adhesion and surface growth of Staphylococcus page 39
epidermidis and Pseudomonas aeruginosa on biomedical
polymers
Chapter 4 Antimicrobial effects of positively charged surfaces on adhering page 55
Gram-positive and Gram-negative bacteria
Chapter 5 Positively charged biomaterials exert antimicrobial effects on page 69
Gram-negative bacilli in rats
Chapter 6 Late hematogenous infection of subcutaneous implants page 77
in rats
Chapter 7 In vitro and in vivo antimicrobial activity of covalently coupled page 89
quaternary ammonium silane coatings on silicone rubber
Chapter 8 General discussion page 101
Summary page 111
Samenvatting voor niet ingewijden page 115
Nawoord page 119

1
Introduction and aims
Bart Gottenbos, Henk J. Busscher, Henny C. van der Mei and Paul Nieuwenhuis
One of the major drawbacks in the use of biomedical materials is the occurrence of
biomaterial-centered infections. After implantation, the host interacts with a biomaterial by
forming a conditioning film on its surface and an immune response towards the foreign
material. When microorganisms can reach the biomaterial surface they can adhere to it.
Adhesion of microorganisms to an implant is mediated by their physico-chemical surface
properties and the properties of the biomaterial surface itself. Subsequent surface growth of
the microorganisms will lead to a mature biofilm and infection, which is difficult to eradicate
by antibiotics. The purpose of this review is to give an overview of the mechanisms involved
in biomaterial-centered infection and the possible strategies to prevent these infections.
Submitted to Journal of Materials Science: Materials in Medicine
9