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UNIVERSIDADE DE TRÁS-OS-MONTES E ALTO DOURO HISTOPATHOLOGY AND IMMUNOHISTOCHEMISTRY CHARACTERIZATION OF MAMMARY LESIONS CHEMICALLY-INDUCED BY 7,12-DIMETHYLBENZ(A)ANTHRACENE AND 1-METHYL-1-NITROSOUREA IN FEMALE SPRAGUE-DAWLEY RATS Thesis in Veterinary Sciences ANTONIETA MARIA ALVARADO MUÑOZ Advisors: Professor Doutor Bruno Jorge Antunes Colaço Professora Doutora Paula Alexandra Martins de Oliveira Professor Doutor António Cabrita Vila Real, November 2017 UNIVERSIDADE DE TRÁS-OS-MONTES E ALTO DOURO HISTOPATHOLOGY AND IMMUNOHISTOCHEMISTRY CHARACTERIZATION OF MAMMARY LESIONS CHEMICALLY-INDUCED BY 7,12-DIMETHYLBENZ(A)ANTHRACENE AND 1-METHYL-1-NITROSOUREA IN FEMALE SPRAGUE-DAWLEY RATS Thesis in Veterinary Sciences ANTONIETA MARIA ALVARADO MUÑOZ Advisors: Professor Doutor Bruno Jorge Antunes Colaço Professora Doutora Paula Alexandra Martins de Oliveira Professor Doutor António Cabrita Composition of the Jury Professor Doutor Jorge Manuel Teixeira de Azevedo Professora Doutora Maria da Conceição da Cunha e Vasconcelos Peleteiro Professor Doutor Rui Pedro Brás Martins Faísca Professora Doutora Anabela Gouveia Antunes Alves Professora Doutora Isabel Cristina Ribeiro Pires Professora Doutora Helena Maria Vala Correia Professor Doutor Bruno Jorge Antunes Colaço Vila Real, November 2017 Original thesis presented by Antonieta María Alvarado Muñoz at the University of Trás-os-Montes and Alto Douro, to obtain the doctor’s degree on Veterinary Science This work was partially funded by Operational Competitiveness and Internationalization Programme, under Project POCI-01- 0145-FEDER-016728, National Funds by FCT-Portuguese Foundation for Science and Technology, under the project PTDC/DTP-DES/6077/2014 and Consejo de Desarrollo Científico, Humanístico y Tecnológico CDCHT, under the project 008REV2015 To My Fathers, Brothers, Nephews and Nieces. My Family VII VIII

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lethal, and mutagenic effects of N-nitroso-N-methylurea. Molecular Carcinogenesis,. 43(3): 155-164. Arpino, G.; Milano, M. and De, P.S. (2015). Features of aggressive breast cancer. Breast,. 24(5): 594-600. Arroyo-Acevedo, J.; Chávez-Asmat, R.J.; Anampa-Guzmán, A.; Donaires, R. and Ráez-
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