ebook img

UltiMate 3000 ACC-3000 Autosampler Column Compartment - Dionex PDF

158 Pages·2013·1.63 MB·English
by  
Save to my drive
Quick download
Download
Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.

Preview UltiMate 3000 ACC-3000 Autosampler Column Compartment - Dionex

Thermo Scientific Dionex UltiMate 3000 Series Autosampler Column Compartment ACC-3000 Operating Instructions (Original Operating Instructions) Revision: 1.4 Date: September 2013 © 2013 Thermo Fisher Scientific Doc No. 4828.3050 UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment Operating Instructions UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Autosampler Column Compartment Types: ACC3000 and ACC-3000T Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Directive 2006/95/EC • EMC Directive 2004/108/EC The electrical safety of the products was evaluated based on the following standard: • DIN EN 61010-1:2010 Safety requirements for electrical equipment for measurement, control and laboratory use, Part 1: General Requirements The Electromagnetic Compatibility (EMC) of the products was evaluated based on the following standard: • DIN EN 61326:2006 Electrical equipment for measurement, control and laboratory use EMC Requirements This declaration is issued for the manufacturer Dionex Softron GmbH Part of Thermo Fisher Scientific Inc. Dornierstraße 4 D-82110 Germering by the Managing Director, Rüdiger Obst and den Vice President HPLC, Fraser McLeod. September 2, 2013 Operating Instructions Page I UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment Page II Operating Instructions UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment Table of Contents 1 Introduction ................................................................................................................... 1 1.1 How to Use This Manual ........................................................................................... 1 1.2 Safety Information ..................................................................................................... 3 1.2.1 Symbols on the Instrument and in the Manual .................................................. 3 1.2.2 Safety Precautions.............................................................................................. 4 1.2.3 Consignes de Sécurité ........................................................................................ 7 1.3 Intended Use ............................................................................................................ 10 1.4 Federal Communications Commission (FCC) Note ................................................ 12 2 Overview ...................................................................................................................... 13 2.1 Unit Description ....................................................................................................... 13 2.2 Operating Principle .................................................................................................. 15 2.3 Autosampler Configurations .................................................................................... 17 2.3.1 Overview .......................................................................................................... 17 2.3.2 Autosampler with Sample Thermostatting Option .......................................... 17 2.3.3 Proper Syringe, Buffer Tubing and Sample Loop Combinations .................... 18 2.4 Interior Components ................................................................................................ 19 2.5 Front Panel Elements ............................................................................................... 21 2.6 Rear Panel ................................................................................................................ 22 2.6.1 Power Switch ................................................................................................... 23 2.6.2 Fuse Cartridge .................................................................................................. 23 2.6.3 USB Port (USB 1.1)......................................................................................... 23 2.6.4 Digital I/O Ports ............................................................................................... 23 2.7 Carousel, Sample Trays, and Vials .......................................................................... 24 2.8 Compartment Lights ................................................................................................ 26 2.9 Fluid Connections .................................................................................................... 26 2.10 Leak Sensor .............................................................................................................. 26 2.11 Injection Valve ......................................................................................................... 27 2.12 Column Chamber ..................................................................................................... 28 2.13 Chromeleon Software .............................................................................................. 29 2.14 System Wellness and Predictive Performance ......................................................... 30 3 Installation ................................................................................................................... 31 3.1 Facility Requirements .............................................................................................. 31 3.2 Unpacking ................................................................................................................ 31 3.3 Positioning the Autosampler in the UltiMate 3000 System .................................... 33 3.4 Connecting the Autosampler ................................................................................... 35 3.4.1 General Information ......................................................................................... 35 3.4.2 Connecting the USB Cable .............................................................................. 35 3.4.3 Connecting the Power Cord ............................................................................. 36 3.4.4 Connecting the Digital I/O ............................................................................... 36 Operating Instructions Page i UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment 3.5 Setting Up the Autosampler in Chromeleon ............................................................ 37 3.5.1 Loading the USB Driver for the Autosampler ................................................. 37 3.5.2 Installing the Autosampler ............................................................................... 39 3.5.3 Configuring the Autosampler........................................................................... 40 3.6 Setting Up the Autosampler in DCMSLink ............................................................. 45 4 Preparation for Operation (Startup) ........................................................................ 47 4.1 Overview of Actions ................................................................................................ 47 4.2 Tips and Precautions for Connecting Capillaries ..................................................... 48 4.3 Connecting the Column ........................................................................................... 50 4.4 Connecting the Wash Liquid Lines .......................................................................... 51 4.4.1 Connecting the Wash Liquid Lines for Online Degassing .............................. 51 4.4.2 Connecting the Wash Liquid Bottle ................................................................. 53 4.5 Connections on the Syringe Valve ........................................................................... 54 4.6 Connecting Drain Tubing......................................................................................... 55 4.7 Removing Air Bubbles from the Syringe (Priming) ................................................ 56 4.8 Installing and Loading the Vial Tray ....................................................................... 57 4.9 Loading the 5-Position Vial Holder ......................................................................... 58 4.10 Equilibrating the System .......................................................................................... 60 5 Operation and Maintenance ...................................................................................... 63 5.1 Power-Up ................................................................................................................. 63 5.2 Status Screen ............................................................................................................ 64 5.3 Sample Positions ...................................................................................................... 65 5.4 Operation from Chromeleon .................................................................................... 67 5.4.1 Connecting to Chromeleon .............................................................................. 67 5.4.2 Direct Control .................................................................................................. 68 5.4.3 Automated Control ........................................................................................... 71 5.5 Display Screens (Function Keys and Menus) .......................................................... 73 5.5.1 Showing the Function Keys ............................................................................. 73 5.5.2 Autosampler Menus ......................................................................................... 75 5.6 Settings for Operating the Autosampler................................................................... 81 5.6.1 Setting the Injection Mode ............................................................................... 81 5.6.2 Linking the Autosampler to the Pump ............................................................. 83 5.6.3 Synchronizing the Inject Command with the Pump Strokes ........................... 83 5.6.4 Turning On Column Thermostatting ............................................................... 84 5.6.5 Turning On Sample Thermostatting ................................................................ 85 5.6.6 Changing the Carousel Configuration.............................................................. 86 5.6.7 Setting the Injection Parameters and Starting the Injection ............................. 86 5.6.8 Detecting Liquid Leaks in the Autosampler .................................................... 88 5.6.9 Adjusting the Screen Brightness or Contrast ................................................... 88 5.6.10 Wash Cycle ...................................................................................................... 89 5.6.11 SmartStartup and SmartShutdown ................................................................... 89 5.6.12 Recommendations for Operation ..................................................................... 89 Page ii Operating Instructions UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment 5.7 Special Chromeleon Functions ................................................................................ 90 5.7.1 Predictive Performance .................................................................................... 90 5.7.2 Defining the Needle Height (Sample Height).................................................. 91 5.7.3 Moving the Tray before Drawing the Sample (Tray Shake) ........................... 93 5.7.4 Venting the Vial ............................................................................................... 93 5.7.5 Operational Qualification and Performance Qualification .............................. 93 5.7.6 Automatic Tray Test ........................................................................................ 93 5.7.7 Automatic Vial Detection ................................................................................ 93 5.8 Shutting Down the Autosampler.............................................................................. 94 5.9 Routine and Preventive Maintenance ...................................................................... 96 6 Troubleshooting .......................................................................................................... 99 6.1 Overview .................................................................................................................. 99 6.2 Messages on the Autosampler Display .................................................................. 100 6.3 Operating Problems ............................................................................................... 105 7 Service ........................................................................................................................ 111 7.1 General Notes and Safety Precautions ................................................................... 111 7.2 Drying the Leak Sensor (Eliminating Leakage) .................................................... 113 7.3 Replacing the Syringe ............................................................................................ 114 7.4 Replacing the Buffer Tubing ................................................................................. 117 7.5 Needle and Needle Capillary ................................................................................. 118 7.5.1 Replacing the Needle or the Needle Capillary............................................... 118 7.5.2 Washing the Needle Manually ....................................................................... 120 7.6 Replacing the Sample Loop ................................................................................... 121 7.7 Injection Valve ....................................................................................................... 122 7.7.1 Inspecting the Injection Valve for Leakage ................................................... 123 7.7.2 Replacing the Valve Pod................................................................................ 124 7.7.3 Replacing the Valve Stator ............................................................................ 126 7.7.4 Replacing the Rotor Seal ............................................................................... 127 7.8 Replacing the Capillary for Eluent Preheating ...................................................... 129 7.9 Replacing the Main Power Fuses........................................................................... 131 7.10 Updating the Autosampler Firmware .................................................................... 132 8 Technical Information .............................................................................................. 135 9 Accessories, Spare Parts, and Consumables .......................................................... 137 9.1 Standard Accessories ............................................................................................. 137 9.2 Optional Accessories ............................................................................................. 139 9.3 Consumables and Spare Parts ................................................................................ 140 Operating Instructions Page iii UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment 10 Injection Modes ......................................................................................................... 143 10.1 Full-Loop Injection ................................................................................................ 143 10.2 Partial-Loop Injection ............................................................................................ 144 11 Appendix - Digital I/O (Pin Assignment) ................................................................ 145 12 Index ........................................................................................................................... 147 Page iv Operating Instructions UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment 1 Introduction 1.1 How to Use This Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of your Thermo Scientific™ Dionex™ ACC-3000 Autosampler Column Compartment, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation. The descriptions in this manual apply to the following instruments in the UltiMate™ 3000 ACC-3000 series: • ACC-3000 • ACC-3000T The following conventions apply to the descriptions throughout this manual: • The term "the device" or the autosampler" is used throughout the manual. If some detail applies to only one autosampler model or one version, the model or version is identified by name. • If not otherwise stated, the descriptions for the ♦ ACC-3000 apply also to the version with sample thermostatting option (ACC 3000T). ♦ Viper™ capillary connections apply also to nanoViper™ and possible other Viper capillary connections. • The device configuration may vary, for example, the autosampler may have a thermostatted carousel. Therefore, not all descriptions necessarily apply to your particular instrument. • The representation of a component in this manual may be different from the real component. However, this does not influence the descriptions. • The descriptions in this manual refer to firmware version 4.13 and Chromeleon™ 6.80 Service Release 13. If you want to operate the autosampler from Chromeleon 7, note the information on page 29. This manual is provided "as is". Every effort has been made to supply complete and accurate information and all technical specifications have been developed with the utmost care. The information contained in this manual should not be construed as a commitment by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any errors that may appear in this document that is believed to be complete and accurate at the time of publication and, in no event, shall Thermo Fisher Scientific be liable for incidental or consequential damages in connection with or arising from the use of this document. We appreciate your help in eliminating any errors that may appear in this document. Operating Instructions Page 1 UltiMate 3000 Series: ACC-3000 Autosampler Column Compartment The information contained in this document is subject to change without notice. All rights reserved, including those for photomechanical reproduction and storage on electronic media. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Thermo Fisher Scientific Inc. Trademarks Analyst is a registered trademark of AB Sciex. Compass and Hystar are trademarks of Bruker Daltonics. Empower is a trademark of Waters Corp. PEEK is a trademark of Victrex PLC. RheBuild is a registered trademark of Idex Corp. Microsoft, Windows, and Windows Vista are registered trademarks of Microsoft Corp. All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries. Page 2 Operating Instructions

Description:
Sep 2, 2013 Thermo Scientific Dionex 1.2.1 Symbols on the Instrument and in the Manual . 2.3.2 Autosampler with Sample Thermostatting Option .
See more

The list of books you might like

Most books are stored in the elastic cloud where traffic is expensive. For this reason, we have a limit on daily download.