Transgenic Plants - Advances and Limitations

490 Pages·2012·21.475 MB·English

by  Y. Ciftci

TRANSGENIC PLANTS –
ADVANCES AND
LIMITATIONS

Edited by Yelda Özden Çiftçi
Transgenic Plants – Advances and Limitations
Edited by Yelda Özden Çiftçi

Published by InTech
Janeza Trdine 9, 51000 Rijeka, Croatia

Copyright © 2012 InTech
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Technical Editor Teodora Smiljanic
Cover Designer InTech Design Team

First published February, 2012
Printed in Croatia

A free online edition of this book is available at www.intechopen.com
Additional hard copies can be obtained from [email protected]

Transgenic Plants – Advances and Limitations, Edited by Yelda Özden Çiftçi
p. cm.
ISBN 978-953-51-0181-9

Contents

Preface IX
Part 1 Application 1
Chapter 1 Agrobacterium-Mediated Transformation of Wheat:
General Overview and New Approaches to Model
and Identify the Key Factors Involved 3
Pelayo Pérez-Piñeiro, Jorge Gago,
Mariana Landín and Pedro P. Gallego
Chapter 2 Recent Advances in Fruit Species Transformation 27
Hülya Akdemir, Jorge Gago,
Pedro Pablo Gallego and Yelda Ozden Çiftçi
Chapter 3 Green Way of Biomedicine –
How to Force Plants to Produce New Important Proteins 63
Aneta Wiktorek-Smagur, Katarzyna Hnatuszko-Konka,
Aneta Gerszberg, Tomasz Kowalczyk,
Piotr Luchniak and Andrzej K. Kononowicz
Chapter 4 Molecular Breeding of Grasses by
Transgenic Approaches for Biofuel Production 91
Wataru Takahashi and Tadashi Takamizo
Chapter 5 Bioactive Beads-Mediated Transformation of Rice with Large
DNA Fragments Containing Aegilops tauschii Genes, with
Special Reference to Bead-Production Methodology 117
Naruemon Khemkladngoen, Naoki Wada, Suguru Tsuchimoto,
Joyce A. Cartagena, Shin-ichiro Kajiyama and Kiichi Fukui
Chapter 6 Genetic Transformation of
Immature Sorghum Inflorescence via
Microprojectile Bombardment 133
Rosangela L. Brandão,
Newton Portilho Carneiro, Antônio C. de Oliveira,
Gracielle T. C. P. Coelho and Andréa Almeida Carneiro
VI Contents

Chapter 7 Phytoremediation of Bis-Phenol A via Secretory Fungal
Peroxidases Produced by Transgenic Plants 149
Tomonori Sonoki, Yosuke Iimura and Shinya Kajita
Chapter 8 Biological Activity of Rehmannia glutinosa
Transformed with Resveratrol Synthase Genes 161
Bimal Kumar Ghimire, Jung Dae Lim and Chang Yeon Yu
Chapter 9 Methods to Transfer Foreign Genes to Plants 173
Yoshihiro Narusaka, Mari Narusaka,
Satoshi Yamasaki and Masaki Iwabuchi
Part 2 Crop Improvement 189
Chapter 10 Genetic Enhancement of
Grain Quality-Related Traits in Maize 191
H. Harting, M. Fracassetti and M. Motto
Chapter 11 Stability of Transgenic Resistance
Against Plant Viruses 219
Nikon Vassilakos
Chapter 12 Expression of Sweet Potato
Senescence-Associated Cysteine Proteases
Affect Seed and Silique Development and
Stress Tolerance in Transgenic Arabidopsis 237
Hsien-Jung Chen, Guan-Jhong Huang, Chia-Hung Lin,
Yi-Jing Tsai, Zhe-Wei Lin, Shu-Hao Liang and Yaw-Huei Lin
Part 3 Metabolomics 257
Chapter 13 Transgenic Plants as a Tool for
Plant Functional Genomics 259
Inna Abdeeva, Rustam Abdeev,
Sergey Bruskin and Eleonora Piruzian
Chapter 14 Transgenic Plants as Gene-Discovery Tools 285
Yingying Meng, Hongyu Li, Tao Zhao, Chunyu Zhang,
Chentao Lin and Bin Liu
Chapter 15 Transgenic Plants as Biofactories for
the Production of Biopharmaceuticals:
A Case Study of Human Placental Lactogen 305
Iratxe Urreta and Sonia Castañón
Chapter 16 Arabinogalactan Proteins in
Arabidopsis thaliana Pollen Development 329
Sílvia Coimbra and Luís Gustavo Pereira
Contents VII

Chapter 17 Trichome Specific Expression:
Promoters and Their Applications 353
Alain Tissier
Chapter 18 Comparative Metabolomics of Transgenic Tobacco Plants
(Nicotiana tabacum var. Xanthi) Reveals Differential Effects
of Engineered Complete and Incomplete Flavonoid Pathways
on the Metabolome 379
Corey D. Broeckling, Ke-Gang Li and De-Yu Xie
Chapter 19 Effect of Antisense Squalene Synthase Gene Expression on
the Increase of Artemisinin Content in Artemisia anuua 397
Hong Wang, Yugang Song, Haiyan Shen, Yan Liu,
Zhenqiu Li, Huahong Wang, Jianlin Chen,
Benye Liu and Hechun Ye
Part 4 Biosafety 407
Chapter 20 Transgenic Plants – Advantages Regarding Their Cultivation,
Potentially Risks and Legislation Regarding GMO’s 409
Pusta Dana Liana
Chapter 21 Biosafety and Detection of
Genetically Modified Organisms 427
Juliano Lino Ferreira,
Geraldo Magela de Almeida Cançado, Aluízio Borém,
Wellington Silva Gomes and Tesfahun Alemu Setotaw
Chapter 22 Elimination of Transgenic Sequences in
Plants by Cre Gene Expression 449
Lilya Kopertekh and Joachim Schiemann
Chapter 23 GMO Safety Assessment-Feasibility
of Bioassay to Detect Allelopathy Using
Handy Sandwich Method in Transgenic Plants 469
Katsuaki Ishii, Akiyoshi Kawaoka and Toru Taniguchi

Preface

“Green revolution” aided to develop enormous number of improved varieties
especially in wheat and rice. Following this revolution, traditional and molecular
breeding that benefited from either the desirable genes available naturally or induction
of mutation in economically valuable species, provided also improved varieties in tree
species. However, with the advent of transgenic technology, it became possible to
introduce foreign genes from other plant species that are cross-incompatible and/or
even from bacteria, fungi, viruses, mice, and humans. Thus, the scientific community
realized the importance of genetically modified (GM) crops, not especially for
supplementation of enough food to the growing population, but also for decreasing
the usage of pesticides and other crop protective chemicals.
Today, GM crops are cultivated in USA, Argentina, Brazil, Canada, China, India,
Paraguay, South Africa, Germany, Spain etc. and numerous studies revealed that
cultivation of GM crops is safe for the environment and usage as food, at least for
approved plants. However, there is still a public concern on GM crops in a number of
countries especially in European Union. The main concerns involve cross-pollination
between GM crops and wild species, the use of especially antibiotic resistance marker
genes, the introduction of possible allergens into the food chain, generation of adverse
effect on non-target organisms. But, all of these concerns caused improvements of the
technology such as development of new marker systems as phosphomannose
isomerase (PMI) and marker-free plants and also production of cisgenic plants.
Moreover, biosafety regulations are also carried out very carefully to prevent its
potential side-effects.
As emerging studies carried on transgenic plants, this book tried to address many
aspects of GM plants including its application on different plant species (i.e., wheat,
fruit trees and sorghum) together with its usage for crop improvement (i.e., insect and
virus resistance, enhancement of quality etc.) and metabolomic studies (i.e., usage for
gene discovery and production of biopharmaceuticals). In addition, the risk
assessment and economical implications of GM crops are also discussed. Thus, this
book is structured into four sections namely, i) Application, ii) Crop Improvement, iii)
Metabolomics, and iv) Biosafety. All of those sections include general and research
papers that are written by scientist who have experience in transgenic technology. I
would like to thank to all of the Authors not only for making this book a valuable
X Preface

recent resource for various aspects of transgenic crops, but also for enabling this Open
Access publication to reach many scientist, teachers, and students working in that
field. Finally, I would also like to thank InTech Publishing Company, especially
Publishing Process Managers Mr Marko Rebrovic and Ms Silvia Vlase, and the
Technical Editor of the book.

Assoc. Prof. Yelda Özden Çiftçi
Gebze Institute of Technology,
Department of Molecular Biology and Genetics,
Kocaeli,
Turkey