Transfer of Cell Constituents into Eukaryotic Cells PDF
Preview Transfer of Cell Constituents into Eukaryotic Cells
Transfer of Cell Constituents into Eukaryotic Cells NATO ADVANCED STUDY INSTITUTES SERIES A series of edited volumes comprising multifaceted studies of contem- porary scientific issues by some of the best scientific minds in the world, assembled in cooperation with NATO Scientific Mfairs Division. Series A: Life Sciences Recent Volumes in this Series Volume 22 - Plant Regulation and World Agriculture edited by Tom K. Scott Volume 23 - The Molecular Biology of Picornaviruses edited by R. Perez-Bercoff Volume 24 - Humoral Immunity in Neurological Diseases edited by D. Karcher, A. Lowenthal, and A. D. Strosberg Volume 25 - Synchrotron Radiation Applied to Biophysical and Biochemical Research edited by A. Castellani and I. F. Quercia Volume 26 - Nucleoside Analogues: Chemistry, Biology, and Medical Applications edited by Richard T. Walker, Erik De Clercq, and Fritz Eckstein Volume 27 - Developmental Neurobiology of Vision edited by Ralph D. Freeman Volume 28 - Animal Sonar Systems edited by Rene-Guy Busnel and James F. Fish Volume 29 - Genome Organization and Expression in Plants edited by C. J. Leaver Volume 30 - Human Physical Growth and Maturation: Methodologies and Factors edited by Francis E. Johnston, Alex F. Roche, and Charles Susanne Volume 31 - Transfer of Cell Constituents into Eukaryotic Cells edited by J. E. Celis, A. Graessmann, and A. Loyter Volume 32 - The Blood-Retinal Barriers edited by Jose G. Cunha-Vaz The series is published by an international board of publishers in con- junction with NATO Scientific Affairs Division A Life Sciences Plenum Publishing Corporation B Physics New York and London C Mathematical and D. Reidel Publishing Company Physical Sciences Dordrecht and Boston D Behavioral and Sijthoff International Publishing Company Social Sciences Leiden Transfer of Cell Constituents into Eukaryotic Cells Edited by 1. E.Celis Aarhus University Aarhus, Denmark A. Graessmann Free University of Berlin Berlin, German Federal Republic and A.Loyter The Hebrew University of Jerusalem Jerusalem, Israel PLENUM PRESS • NEW YORK AND LONDON Published in cooperation with NATO Scientific Affairs Division ISBN 978-1-4684-3709-6 ISBN 978-1-4684-3707-2 (eBook) DOI 10.1007/978-1-4684-3707-2 Lectures presented at a NATO Advanced Study Institute on Transfer of Cell Constituents into Eukaryotic Cells, held in Sintra-Estoril, Portugal, September 2-14, 1979 and sponsored by NATO and The Gulbenkian Foundation © 1980 Plenum Press, New York Softcover reprint of the hardcover 1st edition 1980 A Division of Plenum Publishing Corporation 227 West 17th Street, New York, N.Y. 10011 All righ ts reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfIlming, recording, or otherwise, without written permission from the publisher PREFACE This book is a record of the proceedings of a NATO Advanced Study Institute on "Transfer of Cell Constituents into Eukaryotic Cells" held September 2-14, 1979, at Sintra-Estoril, Portugal. Transfer of cell constituents into living cells can be brought about by a variety of ways, namely by microinjection with micropi- pettes (small somatic cells, oocytes and plant cells), by microin- jection using fusion methods (red cell mediated microinjection, li- posome mediated transfer, transfer of membrane components, cell hy- bridization and fusion of cell fragments)and by means of uptake (DNA and chromosome gene transfer). Prompted by the development~ in this exciting and expanding field of cell biology it seemed timely to meet to review the exist- ing knowledge and to speculate on future research applications. The lectures published here reflect the diversity of research currently underway using these techniques. The final outcome of the meeting was positive as it promoted collaboration between laboratories and, most important, created strong scientific and social links between individuals. We wish to express our appreciation to Dr. Maria C. Lechner and Mrs. Helena Gata for providing valuable advice and help through the initial planning stages of this meeting. Finally, we are indebted to Ms. Lisbeth Heilesen for her outstanding organization and admini- v vi PREFACE stration of the meeting and for typing all the manuscripts of this book. The meeting was held under the sponsorship of NATO with partial support from the Gulbenkian Foundation. J.E. Celis A. Graessmann November 1979 A. Loyter CONTENTS t1ICROINJECTION OF SOMATIC CELLS WITH MICROPIPETTES 1. Microinjection of Somatic Cells with Micropipettes and PEG-Erythrocyte Ghost ~lediated Microinjection ..........•..................... J.E. Celis~ K. Kaltoft and R. Bravo 2. Microinjection of Cellular and Viral mRNAs 29 D. W. Stacey 3. Expression of Rabbit Globin mRNA Injected into Fused HeLa Cells .................................. 55 G. Huez~ C. Bruck~ D. Portetelle and Y. Cleuter 4. Biological Activity of Simian Virus 40 DNA Fragments and T-Antigen Tested by t1icro- injection into Tissue Culture Cells ............ ........ 61 A. Graessmann~ M. Graessmann and C. Mueller 5. Aspects of Cell Architecture and Locomotion .... ... ..... 75 J.V. Small~ J.E. Celis and G. Isenberg GHOST MEDIATED MICROINJECTION AND TRANSFER OF MEMBRANE COMPONENTS 6. Red Cell-Mediated Microinjection Studies on Protein Degradation .................................... 113 M. Rechsteiner vii viii CONTENTS 7. Transfer of Functional Compoments into Plasma Membrane of Living Cells: A New Tool in Membrane Research ...................................... 143 A.Loyter~ D.J. Volsky~ M. Beigel~ B. Ginsberg and Z.I. Cabantchik LIPOSOME MEDIATED TRANSFER 8. Liposomes as Macromolecular Carriers for the Introduction of RNA and DNA into Cells 155 D. Papahadjopoulos~ T. Wilson and R. Taber 9. Liposomes in Drug Targeting .. ............ .............. 173 G. Gregoriadis FUSION OF CELL FRAGMENTS AND CELL HYBRIDS 10. Fusion of Cell Fragments as a Method in Cell Genetics....................................... 201 T. Ege 11. B-Cell and Epstein-Barr Virus (EB) Associated Functions in Human Cells and Hybrids................... 235 J. Zeuthen and G. Klein 12. Tumorigenicity, Actin Cables and Gene Expres- sion in Mouse CLIO x CHO Cell Hybrids 275 R. Bravo~ J.V. Small~ A. Celis~ K. Kaltoft and J.E. Celis DNA AND CHROMOSOME MEDIATED GENE TRANSFER 13. Gene Transfer in Eukaryotes .. 0 ••••••••••••••••••••••••• 295 F.B. Ruddle 14. DNA Mediated Gene Transfer between ~·1amma 1 i an Cell s .................... 0 ••••••••••••••••• 0 • 311 K. Willecke CONTENTS ix 15. Transfer of DNA into Plant Cells with the Ti-Plasmid as a Vector 325 J. Schell and M. Van Montagu ~lICROINJECTION OF XENOPUS OOCYTES 16. Expression of Messenqer RNAs Injected into Xenopus Laevis Oocytes ............................ 347 Go Marbaix and G. Huez 17. Surrogate Genetics in the Frog Oocyte 383 A. Kressmann and M.L. Birnstiel TRANSFER OF CELL CONSTITUENTS INTO PLANT CELLS 18. Grafts and Transfer of Cell Constituents into the Giant Unicellular Alga Acetabularia ..............•• 409 S. Bonotto and A. Luttke Contri butors ..... 0 ••••••••••• 00 ••• 0 • • • • • • • • • • • • • • • • • • • • • • • • 433 Subject Index ................ 0 •••••••••••••••••••••••••••••• 437
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