Topics and Perspectives in Adenosine Research: Proceedings of the 3rd International Symposium on Adenosine, Munich, June 1986

641 Pages·1987·19.216 MB·English

by  J. Linden, C. Q. Earl, A. Patel, R. H. Craig, S. M. Daluge (auth.), Professor Dr. med. Eckehart Gerlach, Dr rer nat. Bernhard Friedrich Becker (eds.)

.T opics
and Perspectives
in Adenosine
Research
Proceedings of the 3rd International
Symposium on Adenosine,
Munich, June 1986
E. Gerlach B.F.Becker (Eds.)
With 218 Figures and 64 Tables
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Professor Dr. med. Eckehart Gerlach
Dr. rer. nat. Bernhard Friedrich Becker
Physiologisches Institut der UniversiHit Miinchen
PettenkoferstraBe 12
8000 Miinchen 2
Federal Republic of Germany
ISBN- 13 :978-3-642-45621-3 e-ISBN-13 :978-3-642-45619-0
DOl: 10.1007/978-3-642-45619-0
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© Springer-Verlag Berlin Heidelberg 1987
Softcover reprint of the hardcover 1 st edition 1987
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Preface
An "International Conference on Physiological and Regulatory Functions of
Adenosine and Adenine Nucleotides" was held in Banff, Canada, in June 1978.
Four years later, in June 1982, an "International Symposium on Adenosine" took
place in Charlottesville, Virginia, USA. Both meetings revealed an increasing
interest of scientists from numerous disciplines on biochemical, physiological
and pathophysiological aspects of adenosine as a proposed mediator of various
specified regulatory functions, both on the cellular and organ level. In view of the
obvious continuity of adenosine research in well-established and newly emerging
fields, a scientific committee was charged with organizing a further meeting to
be held in Munich, Germany, in June 1986. Members of the committee were
H. P. Baer, Canada, R. M. Berne, USA, and E. Gerlach, G. W. Kreutzberg and
J. Schrader, FRG. It was the general intention that in this conference, purposely
named "3rd International Symposium on Adenosine", emphasis should be
placed on the presentation of shorter reports concerning both latest as well as
controversial aspects and developments in as wide a scope of adenosine research
as possible. Therefore, in addition to more than 50 presentations by invited
speakers, over 120 posters were displayed. Coinciding with the symposium,
abstracts of all contributions were published in Pfltigers Archiv - European
Journal of Physiology [Vol. 407, Suppl. 1, 1986].
This book, entitled "Topics and Perspectives in Adenosine Research", now
enholds all papers presented at the 3rd International Symposium on Adenosine,
together with the complete discussions following each talk. In order to make
these proceedings available as early as possible, the manuscripts are published as
submitted by the authors, except for copy-editing by the publishing company;
standardization of abbreviations was not attempted. The transcripts of the oral
discussions were edited where necessary. The chapters of the book are arranged
according to the topics of the symposium; the sequence of contributions had to
be modified only in a few cases.
The editors are indebted to Dr. J. Wieczorek, Springer-Verlag, for his thoughtful
cooperation in the publication of this book.
We hope that these proceedings will help to update and to define the state of the
art in major fields of adenosine research - at least for the year 1986 - and will be
of value to a broader scientific community.
Munich, February 1987 E. Gerlach and B. F. Becker
Acknowledgements
The "3rd International Symposium on Adenosine" was very generously sponso
red by
Pharma Schwarz GmbH, Monheim, FRG.
Further support was kindly provided by
Boehringer Mannheim GmbH, Mannheim, FRG,
G6decke AG, Freiburg i. Br., FRG,
Hoechst AG, Werk Albert, Wiesbaden, FRG,
Paul-Martini-Stiftung, Mainz, FRG,
Pfrimmer + Co., Pharmazeutische Werke, Erlangen, FRG,
Sandoz AG, Niirnberg, FRG,
Schering AG, Berlin,
Dr. Willmar Schwabe, Karlsruhe, FRG,
Springer-Verlag, Berlin Heidelberg, FRG,
Dr. Karl Thomae GmbH, Biberach/Riss, FRG,
Warner-Lambert Company, Ann Arbor, USA.
Venue and splendid facilities for the meeting were obligingly made available by
the
Max-Planck-Institute, Martinsried, FRG.
On behalf of the Scientific Committee, thanks are once again conferred to all the
sponsoring institutions and companies as well as to Mr. M. Giicker, Pharma
Schwarz GmbH, for his personal engagement, to Mrs. B. Kratzenberg for her
excellent assistance in organisational matters, and to Mrs. K. Schaipp and Mrs.
M. Bellstedt for their invaluable, conscientious secretarial help.
E. Gerlach
Table of Contents
I. Adenosine Receptors and Nncleoside Transporter
Agonist and Antagonist Radioligands and Photo affinity Labels for the
Adenosine Al Receptor
J. Linden, C. Q. Earl, A. Patel, R. H. Craig, and S. M. Daluge . . . . . 3
Functional Characterization of Al Adenosine Receptors by Photoaffinity
Labeling
M. J. Lohse, K-N. Klotz, and U. Schwabe .................. 15
Analogues of Adenosine, Theophylline, and Caffeine: Selective Interactions
with Al and A2 Adenosine Receptors
J. W. Daly, D. Ukena, and K A. Jacobson .................. 23
Structural Studies on Adenosine Ri Receptors from Rat Cerebral Cortex
E. Perez-Reyes, S.-M. H. Yeung, R. L. Lasher, and D. M. F. Cooper . .. 37
Biochemical and Autoradiographic Approaches to the Characterization of
Adenosine Receptors in Brain
M. Reddington, S. P. Alexander, A. Erfurth, K S. Lee, and G. W. Kreutzberg 49
Adenosine Receptor Subtypes: Binding Studies
R. F. Bruns, G. H. Lu, and T. A. Pugsley .................... 59
Central Sites of Adenosine Action and Their Interaction with Various Drugs
P. J. Marangos, J. Deckert, and J.-c. Bisserbe ................. 74
Nucleoside Transport Inhibition In Vitro and In Vivo
A. R. P. Paterson, E. S. Jakobs, C. Y. C. Ng, R. D. Odegard, and A. A. Adjei. 89
VIII Table of Contents
Kinetic and Molecular Properties of Nucleoside Transporters in Animal Cells
S. M. Jarvis .................................... 102
Drug Interactions with Nucleoside Transport Systems
A. S. Clanachan, T. P. Heaton, and F. E. Parkinson ............. 118
II. Adenosine Formation, Metabolism and Transport
Regulation and Function of 5'-Nucleotidases
A. R. Collinson, K. J. Peuhkurinen, and J. M. Lowenstein ......... 133
Kinetics of Endothelial Cell Ectonucleotidases
J. D. Pearson, and S. B. Coade ........ . . 145
Critical Evaluation of the Role of Ecto- and Cytosolic 5'-Nucleotidase in
Adenosine Formation
A. C. Newby, Y. Worku, and P. Meghji .................... 155
New Insights into the Mechanism of Myocardial Adenosine Formation
A. Dendorfer, S. Lauk, A. Schaff, and S. Nees . . . . . . . . . . . . .. . 170
Cardiac Myocyte and Coronary Endothelial Cell Adenosine Transport
M. J. Rovetto, D. A. Ford, and A. Yassin ............. . . . 188
The Importance of the Transmethylation Pathway for Adenosine
Metabolism in the Heart
H. G. E. Lloyd, and J. Schrader .................. . ... 199
Uric Acid, the Major Catabolite of Cardiac Adenine Nucleotides and
Adenosine, Originates in the Coronary Endothelium
B. F. Becker, and E. Gerlach .......................... 209
Thermodynamic State of Cytosolic Adenylates in Guinea Pig Myocardium.
Energy-Linked Adaptive Changes in Free Adenylates and Purine
Nucleoside Release
R. Bunger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223
Table of Contents IX
III. Functions of Adenosine in Cells, Organs and Blood
On Multiple Targets for Fat Cell Receptors
C. Londos .................. . . .............. 239
The Renal and Epithelial Actions of Adenosine
W. S. Spielman, L. J. Arend, and J. N. Forrest, Jr. .............. 249
Adenosine and Thermogenesis in Brown Adipose Tissue: Interaction with
Beta and Alpha Adrenergic Responses
R. J. Schimmel, M. E. Elliott, and L. McCarthy ............... 261
The Hormone-Modulatory Effects of Adenosine in Skeletal Muscle
R. A. J. Challiss, B. Leighton, F. J. Lozeman, and E. A. Newsholme . 275
Effects of Adenosine on Human Neutrophil Function and Cyclic AMP
, Content
M. A. Iannone, T. P. Zimmerman, R. Reynolds-Vaughn, and G. Wolberg 286
A New Function for Adenosine: Protection of Vascular Endothelial Cells
from Neutrophil-Mediated Injury
B. N. Cronstein, R. I. Levin, J. Belanoff, G. Weissmann, and R. Hirschhorn. 299
Formation of Adenosine by Vascular Endothelium: a Homeostatic and
Antithrombogenic Mechanism?
E. Gerlach, B. F. Becker, and S. Nees ..................... 309
IV. Adenosine in the Heart and Vascular System
Ionic Channels and Adenosine in Isolated Heart Cells
G. Isenberg, E. Cerbai, and U. KlOckner ... 323
The Negative Chronotropic Effect of Adenosine in Sinus Node Cells
G. A. West, W. Giles, and L. Belardinelli .......... . ... 336
Regulation of Atrioventricular Node Function by Adenosine
L. Belardinelli, G. A. West, and S. H. F. Clemo ............... 344
X Table of Contents
The Antiadrenergic Actions of Adenosine in the Heart
J. G. Dobson, Jr., R. A. Fenton, and F. D. Romano .. . 356
Mechanism of the "Antiadrenergic" Effects of Adenosine on Myocardial
Force of Contraction
H. Scholz, M. Bohm, R. Briickner, J. Neumann, and W. Schmitz ..... 369
Further Evidence Against Adenosine-Catecholamine Antagonism In Vivo:
Investigations with Treadmill Exercise in Dogs
G. Raberger, G. Fischer, G. Krumpl, W. Schneider, and H. Stroifinig ... 383
Adenosine in the Local Regulation of Blood Flow: Some Controversies
R. M. Berne, J. M. Gidday, H. E. Hill, R. R. Curnish, and R. Rubio .... 395
Adenosine in the Local Regulation of Blood Flow: Current Controversies
H. V. Sparks, Jr., and M. W. Gorman . . . . . . . . . . . . . . . . . . .. 406
5'-Nucleotidase Inhibitors and the Myocardial Reactive Hyperemia and
Adenosine Content
S. Imai, M. Nakazawa, H. Imai, and H. Jin .................. 416
Estimates of Interstitial Adenosine in Isolated Rat Hearts from Surface
Exudates During Rapid Pacing and EHNA Infusion
L. J. Heller, and D. E. Mohrman ........................ 425
Intra-Arterial Adenosine Deaminase: a Tool for Assessing Physiological
Functions of Adenosine
R. A. Olsson ................................... 438
A Critique on the Use of Adenosine Deaminase to Test the Adenosine
Hypothesis: Disregarded Implicit Assumptions
R. Rubio, R. M. Knabb, S. W. Ely, and R. M. Berne . . . . . . . . . . . . . 445
Adenosine Receptors at the Coronary Endothelium: Functional
Implications
S. Nees, Ch. Des Rosiers, and M. Bock . . . . . . . . . . . . . . . . . . . . 454