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Theriogenology TABLE OF CONTENTS January (1) 1995, Volume 43, Number 1 ASSISTED REPRODUCTIVE TECHNOLOGY IN NONDOMESTIC UNGULATES: A MODEL APPROACH TO PRESERVING AND MANAGING GENETIC DIVERSITY N.M. Loskutoff, P. Bartels, M. Meintjes,R.A. Godke and M.C. Schiewe........ APPLICATION OF REPRODUCTIVE TECHNOLOGIES IN NORTH AMERICAN BISON (Bison bi ie Nn sa Gbatinn db on eda eeeeheenesss0bnssssendenstdnbdaddsentssesessnvcoccnecsovell THE APPLICATION OF NEW REPRODUCTIVE TECHNOLOGIES TO SOUTH AMERICAN CAMELIDS M.R. Del Campo, C.H. Del Campo, G.P. Adams and R.J. Mapletoft.....ccccececceeeeesenl EXOGENOUS CONTROL OF FOLLICULAR WAVE EMERGENCE IN CATTLE G.A. Bo, G.P. Adams, R.A. Pierson and R.Jd. Mapletoft.....cceccesccecccscccccessessal THE EFFECTS OF EXOGENOUS GONADOTROPINS ON OOCYTE AND EMBRYO QUALITY IN CATTLE T. Greve, H. Callesen, P. Hyttel. R. Hoier and R. ASS€Y...ccccscecccccccccccceeeecdl POTENTIAL BENEFITS OF BOVINE EMBRYO-MANIPULATION TECHNOLOGIES TO GENETIC IMPROVEMENT PROGRAMS MM. Lohmisccccccccccccccsccccccccceccece cocccccccce wcvccccccccccdl USE OF DNA MARKERS IN ANIMAL SELECTION M.D. Bishep, GA. Mawkins amd C.L. Reefer. ccccccccccccccccccccccccccccccccccccccceOl THE SEXING OF BOVINE EMBRYOS IN THE FIELD M. Thibier and M. Nibert.ccccccccscccccccccccccccdecccccccccescccccccccceseccocccolh EMBRYO TRANSFER AND DISEASE TRANSMISSION IN LIVESTOCK: A REVIEW OF RECENT RES ts TE 6 66.900: 00-00b46000005409000000066066s606 edb Seed eEdededeeseocoeeseoooncst l EPIDEMIOLOGICAL IMPLICATIONS OF THE PRODUCTION AND TRANSFER OF IVF EMBRYOS D.A. Stringfellow and A.E. Wrathall ° ° EMBRYO IMPORTATION AND CRYOBANKING STRATEGIES FOR LABORATORY ANIMALS AND WILDLIFE SPECIES M.C. Schiewe, V.M. Hollifield, L.A. Kasbohm and P.M. Schmidt.........eceeeeeeeeeee97 COMPUTER-ASSISTED IMAGE ANALYSIS, DIAGNOSTIC ULTRASONOGRAPHY AND OVULATION INDUCTION: STRANGE BEDFELLOWS RA. Pherae aml BoP. ABB eccccvccvccccccccccccccccsccoccoscscccoccoscscccoescIcOsS BOVINE MODEL FOR STUDY OF OVARIAN FOLLICULAR DYNAMICS IN HUMANS GA, Adee GRE RA. PIOPEMe cc cccccccccccccccccccvceccccccccececccccccccecccoescelse COLOR DOPPLER IMAGING IN THE EVALUATION OF NORMAL AND ABNORMAL EARLY UTEROPLACENTAL CIRCULATION AND GESTATIONAL OUTCOMES Be GUO hbo cc eSe tes redeceesescageedcoevensedescocdsedecscoscoccscosooccceeosenel ATYPICAL ACTIVATION AND FERTILIZATION PATTERNS IN HUMANS J. Cohen, J. Levron, G.D. Palermo, S. Munne, A. Adler, M. Alikani, G. Schattman, K. Sultan and S. Willadsen.......ccesccsccceccsceecesesled PRODUCTION, FREEZING AND TRANSFER OF BOVINE IVF EMBRYOS AND SUBSEQUENT CALVING RESULTS J.F. Hasler, W.B. Henderson, P.J. Hurtgen, Z.Q. Jin, A.D. McCauley, S.A. Mower, B. Neely, L.S. Shuey, J.E. Stokes and S.A. Trimmer.......eeeeeeeeeeeel4l POST-THAW SURVIVAL AND PREGNANCY RATES OF BIOPSIED, SEXED AND VITRIFIED BOVINE IVF EMBRYOS Y. Agca, R.L. Monson, D.L. Northey, D.M. Schaefer and J.J. Rutledge.............-153 GONADOTROPIN-RELEASING HORMONE-LIKE FACTORS IN THE SEMINAL PLASMA OF THE DROMEDARY CAMEL (Camelus dromedar ius) ME.08. Abfuradsi ame Lehn MOUSERc cccccccccccccccccccccccccceccecscces PTTTTT TTTTT CRYOPRESERVATION OF ENCAPUSULATED MOUSE EMBRYOS J.F. Aller, R.H. Alberio. B. lovannitti and J. Cabodevila........sseeeeeees THE EFFECT OF NEUTRALIZATION OF BVD/MD VIRUS PRESENT IN BOVINE SEMEN ON THE IVF AND DEVELOPMENTO F BOVINE EMBRYOS M. Allietta, B. Guerin. B. Marquant-Le Guienne and M. Thibier........ coccoccoseselee EFFECT OF ENVIRONMENTAL POLLUTANTS ON MATURATION OF BOVINE OOCYTES IN VITRO RB. Ale, BH. Dormer al U. Tice cccccccccccccccccccccccoscccoscoccceceoocosccocelet Theriogenology TABLE OF CONTENTS January (1) 1995, Volume 43, Number 1 ASSISTED REPRODUCTIVE TECHNOLOGY IN NONDOMESTIC UNGULATES: A MODEL APPROACH TO PRESERVING AND MANAGING GENETIC DIVERSITY N.M. Loskutoff, P. Bartels, M. Meintjes,R.A. Godke and M.C. Schiewe........ APPLICATION OF REPRODUCTIVE TECHNOLOGIES IN NORTH AMERICAN BISON (Bison bi ie Nn sa Gbatinn db on eda eeeeheenesss0bnssssendenstdnbdaddsentssesessnvcoccnecsovell THE APPLICATION OF NEW REPRODUCTIVE TECHNOLOGIES TO SOUTH AMERICAN CAMELIDS M.R. Del Campo, C.H. Del Campo, G.P. Adams and R.J. Mapletoft.....ccccececceeeeesenl EXOGENOUS CONTROL OF FOLLICULAR WAVE EMERGENCE IN CATTLE G.A. Bo, G.P. Adams, R.A. Pierson and R.Jd. Mapletoft.....cceccesccecccscccccessessal THE EFFECTS OF EXOGENOUS GONADOTROPINS ON OOCYTE AND EMBRYO QUALITY IN CATTLE T. Greve, H. Callesen, P. Hyttel. R. Hoier and R. ASS€Y...ccccscecccccccccccceeeecdl POTENTIAL BENEFITS OF BOVINE EMBRYO-MANIPULATION TECHNOLOGIES TO GENETIC IMPROVEMENT PROGRAMS MM. Lohmisccccccccccccccsccccccccceccece cocccccccce wcvccccccccccdl USE OF DNA MARKERS IN ANIMAL SELECTION M.D. Bishep, GA. Mawkins amd C.L. Reefer. ccccccccccccccccccccccccccccccccccccccceOl THE SEXING OF BOVINE EMBRYOS IN THE FIELD M. Thibier and M. Nibert.ccccccccscccccccccccccccdecccccccccescccccccccceseccocccolh EMBRYO TRANSFER AND DISEASE TRANSMISSION IN LIVESTOCK: A REVIEW OF RECENT RES ts TE 6 66.900: 00-00b46000005409000000066066s606 edb Seed eEdededeeseocoeeseoooncst l EPIDEMIOLOGICAL IMPLICATIONS OF THE PRODUCTION AND TRANSFER OF IVF EMBRYOS D.A. Stringfellow and A.E. Wrathall ° ° EMBRYO IMPORTATION AND CRYOBANKING STRATEGIES FOR LABORATORY ANIMALS AND WILDLIFE SPECIES M.C. Schiewe, V.M. Hollifield, L.A. Kasbohm and P.M. Schmidt.........eceeeeeeeeeee97 COMPUTER-ASSISTED IMAGE ANALYSIS, DIAGNOSTIC ULTRASONOGRAPHY AND OVULATION INDUCTION: STRANGE BEDFELLOWS RA. Pherae aml BoP. ABB eccccvccvccccccccccccccccsccoccoscscccoccoscscccoescIcOsS BOVINE MODEL FOR STUDY OF OVARIAN FOLLICULAR DYNAMICS IN HUMANS GA, Adee GRE RA. PIOPEMe cc cccccccccccccccccccvceccccccccececccccccccecccoescelse COLOR DOPPLER IMAGING IN THE EVALUATION OF NORMAL AND ABNORMAL EARLY UTEROPLACENTAL CIRCULATION AND GESTATIONAL OUTCOMES Be GUO hbo cc eSe tes redeceesescageedcoevensedescocdsedecscoscoccscosooccceeosenel ATYPICAL ACTIVATION AND FERTILIZATION PATTERNS IN HUMANS J. Cohen, J. Levron, G.D. Palermo, S. Munne, A. Adler, M. Alikani, G. Schattman, K. Sultan and S. Willadsen.......ccesccsccceccsceecesesled PRODUCTION, FREEZING AND TRANSFER OF BOVINE IVF EMBRYOS AND SUBSEQUENT CALVING RESULTS J.F. Hasler, W.B. Henderson, P.J. Hurtgen, Z.Q. Jin, A.D. McCauley, S.A. Mower, B. Neely, L.S. Shuey, J.E. Stokes and S.A. Trimmer.......eeeeeeeeeeeel4l POST-THAW SURVIVAL AND PREGNANCY RATES OF BIOPSIED, SEXED AND VITRIFIED BOVINE IVF EMBRYOS Y. Agca, R.L. Monson, D.L. Northey, D.M. Schaefer and J.J. Rutledge.............-153 GONADOTROPIN-RELEASING HORMONE-LIKE FACTORS IN THE SEMINAL PLASMA OF THE DROMEDARY CAMEL (Camelus dromedar ius) ME.08. Abfuradsi ame Lehn MOUSERc cccccccccccccccccccccccccceccecscces PTTTTT TTTTT CRYOPRESERVATION OF ENCAPUSULATED MOUSE EMBRYOS J.F. Aller, R.H. Alberio. B. lovannitti and J. Cabodevila........sseeeeeees THE EFFECT OF NEUTRALIZATION OF BVD/MD VIRUS PRESENT IN BOVINE SEMEN ON THE IVF AND DEVELOPMENTO F BOVINE EMBRYOS M. Allietta, B. Guerin. B. Marquant-Le Guienne and M. Thibier........ coccoccoseselee EFFECT OF ENVIRONMENTAL POLLUTANTS ON MATURATION OF BOVINE OOCYTES IN VITRO RB. Ale, BH. Dormer al U. Tice cccccccccccccccccccccccoscccoscoccceceoocosccocelet Theriogenology FOLLTROPIN-V-STIMULATED MULTIPLE FOLLICLES CANNOT BE MAINTAINED BY LOW PROGESTERONE IN COWS J.D. Ambrose and R. Rajamahendran......cccccccccccccccccccceccccccccecccccccesceclS S THE USE OF DIFFERENT PROCESSING TREATMENTS AND THEIR EFFECT ON POST-THAW MOTILITY OF CRYOPRESERVED STALLION SPERMATOZOA RK. AnGeshe ame C. Phamt@ic.cccc0c000000s0000s0enh000ccceeseereeebsoseeneoeeewcoonlee IN VITRO CULTURE OF BOVINE EMBRYOS IN MBMOC AND CR2 A. Applewhite and M. Westhusin. ..cccccccccccccccccccccccccccccccccccccccccccccccolGl EFFECT OF SERUM-FREE MEDIUM ON MORPHOLOGY AND PROTEIN SYNTHESIS OF MOUSE EMBRYOS IN CULTURE 3. Apeiee,, Bs Teme aR BD. Termes occ o0000r00 05 00066sncsseskectensocsessceseensln TRANSVAGINAL BGG RETRIEVAL AND IN-VITRO EMBRYO PRODUCTION IN GAUR (Bos gaurus) WITH ESTABLISHMENTO F INTERSPECIES PREGNANCY D.L. Armstrong, C.R. Looney, B.R. Lindsey, C.L. Gonseth, D.L. Johnson, K.R. Williams, L.G. Simmons and N.M. Loskutoff.......ceeeeeeeeeeee1e6 2 A DOSE-RESPONSE STUDY OF IVERMECTIN IN A BOVINE IN VITRO PRODUCTION SYSTEM B. Avery and M. Schmidt.......ccccccccccccccees cccccvccsccocceeeasccoosssooonee IN VITRO EFFECTS OF DIFFERENT DOSES OF FSH ON OOCYTE MATURATION AND EMBRYO DEVELOPMENT JL. Baranao and D.F. Salamamecccccccccccccccccccccccccccccceccocccecccccovceccconee ANTI-eOG ANTIBODIES ARE TRANSMITTED VIA THE COLOSTRUM IN GOATS J.F. Beckers, B. Remey, G. Baril, J.R. Figueiredo, F. Bureau, J. Sulon and J. Saumamnde....cccccccccccccccccccccvcccccccccscccsccceccelGe PREGNANCIES FOLLOWING THE TRANSFER OF IN VITRO MATURED AND FERTILIZED RED DEER (Cervus elaphus) OOCYTES D.K. Berg, G.W. Asher, P.A. Pugh, H.R. Tervit and J.G. Thompson........-+++++++++166 COMPARATIVE EPIDIDYMAL SPERM CELL MOTILITY OF AFRICAN UNGULATE AND BQUID GAME SPECIES STORED AT 4°C C. Bezuidenhout, F. le R. Fourie, M. Meintjes, M.S. Bornmman, P. Bartels and R.A.Godke......ccecceccccceccecsesceeeslOl IN VITRO PRODUCTION OF BOVINE EMBRYOS: DEVELOPMENTAL COMPETENCE IS ACQUIRED BEFORE MATURATION P. Blondin, L.A. Guibault and M.A. Sirard.....cccccccccccccccccccccscesecsccseesslOB DEVELOPMENT OF THE PRESENCE OF A CL IN THE OVARY ON OOCYTE NUMBER, CLEAVAGE RATE AND BLASTOCYSTS PRODUCTION IN VITRO IN CATTLE A. Boediono, R. Rajamahendran, S. Saha. C. Sumantri and T. Suzuki.........+++++++169 EFFECT OF GLYCINE AND ALANINE ON CO-CULTUREO F BOVINE BLASTOCYSTS BR. Geniitehsi, W.. Geek and B.d. Gabba ccccocccccdcccesccccccsnecessscsccescovecdl e RECIPIENT SYNCHRONISATION AND EMBRYO TRANSFER IN SOUTH AMERICAN CAMELIDS D.A. Bourke, C.E. Kyle, T.G. McEvoy, P. Young and C.L. Adam.....ccseecceseceseceelll NUCLEAR AND CYTOPLASMIC MATURATION OF OOCYTES ASPIRATED FROM LARGE FOLLICES IN SUPEROVULATED HEIFERS D. Bousquet, C. Milovanov, J.C. Bell. J. Durocher and L.C. Smith.........+0-++++-e172 THE INVOLVEMENT OF CARBOHYDRATE BINDING SITES IN BOVINE SPERM ACROSOME REACTION A. Brandelli, D.F. Salamone and J.G. TeZon....cccccccccccceccccccesesescsessseseelid EFFECT OF POLYVINYLPYRROLIDONE, SERUM ALBUMIN AND FETAL CALF SERUM ON EARLY CLEAVOFA GBOVEIN E EMBRYOS K. Bredbacka and P. Bredbacka...ccccccccccccccccccccccccccccccccccccsccccccccccoonte MAMMARY GLAND SPECIFIC EXPRESSION OF CHYMOSIN CONSTRUCTS IN TRANSGENIC RABBITS G. Brem, U. Besenfelder, N. Zinovieva, J. Seregi, L. Solti, and P. Hartl.........175 SUPEROVULATORY RESPONSES IN CATTLE PRE-TREATED WITH ESTRADIOL AND PROGESTAGEN P.J. Broadbent, F.E. Gebbie, D.F. Dolman, R.G. Watt, M.E. King and L.C. Higgins..176 TRANSVAGINAL ULTRASOUND-GUIDED OOCYTE COLLECTION IN 10 TO 16 WEEKS OF AGE CALVES G.M. Brogliatti, C.D. Swan and G.P. Adams.ccccccccccccccccccccccccccccccccccocccoltt EFFECTS OF SERUM REPLACERS ON BOVINE GRANULOSA CELL PROLIFERATION DURING EXTENDED CULTURE J.R. Broussard, J.K. Thibodeaux, M.W. Myers, J.D. Roussel and W. Hansel..........178 1434 Theriogenology NORMAL OVINE EMBRYO DEVELOPMENT FOLLOWING IN VITRO OOCYTE MATURATION IN A PORTABLE INCUBATOR IN THE ABSENCE OF 002 S.R. Byrd, G. Flores-Foxworth and M.E. Westhusin.....cccccccccccccccccccccsecceesll® TRANSIENT TRANSFORMATION OF SHRIMP (Penaeus schmitti) EMBRYOS BY DNA MICROINJECTION E. Cabrera, R. Pimentel, Z. Abad, J.C. Pina, O. Hernandez, ie, Ree GR Se Ge Bh PO ec ccccvccccccccctebstencsdsesecndcscececcoesoceseRol PRODUCTION OF LIVE LAMBS FOLLOWING NUCLEAR TRANSFER OF CULTURED EMBRYONIC DISC CELLS K. Campbell, J. McWhir, B. Ritchie and 1. Wilmut.......cccccccccccccccccccccceseslBl EFFECT OF FOLLICLE SIZE AND QUALITY ON THE ABILITY OF FOLLICULAR FLUID TO SUPPORT CYTOPLASMIC MATURATION OF BOVINE OOCYTES C. Carolan, P. Lonergan, P. Monget, D. Monniaux and P. Mermillod...........+++++-182 EFFECTS OF STAGE OF DEVELOPMENTO N SEX RATIOAN D SURVIVAL AFTER FREEZIOFN GDA Y 7 BOVINE IVF EMBRYOS R.V. Carvalho, M.R. Del Campo, Y. Plante and R.J. Mapletoft........ceeececeeeeees183 SECRETION OF HUMAN ERYTHROPOETIN BY MAMMARY GLAND EXPLANTS FROM LACTATING TRANSGENIC RABBITS F.O. Castro, A. Aguirre, P. Fuentes, B. Ramos, A.Rodriguez and J.de la Fuente....184 DEVELOPMENT OF A NOVEL BIOASSAY FOR MEASURING FSH ACTIVITY P.J. Chedrese, G.T. Braileanu and C. Albanese........ceccccccccceees cocccccete® EFFECTS OF CUMULUS CELLS AND HORMONES DURING IN VITRO MATURATION ON THE PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES R.C. Chian and M.A. Sirard........se+6. CeccccecsecesccccocococcscocoeccooS DEVELOPMENT AND OUTGROWTH OF MOUSE EMBRYOS IN WHITTEN’ S MEDIUM SUPPLEMENTED WITH HUMAN AMNIOTIC FLUID (hAF) K.S. Chung, J.H. Kim, K.R. Seung and H.T. Lee........ EFFECT OF ATRESIA ON THE ABILITY OF FOLLICULAR FLUID TO SUPPORT DEVELOPMENTAL COMPETENCE OF SHEEP OOCYTES IN VITRO Y. Cognie, N. Poulin, C. Pisselet and D. Monnaiux........ ° EFFECTS ON EMBRYO DEVELOPMENT OF HEAT TREATMENT AND FILTRATION OF BOVINE FOLLICULAR FLUID USED TO SUPPLEMENT IVM MEDIUM Ae GORDI Ge Rc TEs DieccccccvccccescccsccccesesesccccececesssecscooccScD DIRECT REHYDRATION OF IVM, IVF AND IVC BOVINE EMBRYOS FROZEN IN ETHYLENE-GLYOCOL S. Cseh, U. Kreysing, A. Lucas-Hahn and H. Niemann.......ccccscsceccceccceceseess19O EVALUATION OF CYTOPLASMIC MATURATION OF CALF OOCYTES P. Damiani, R.A. Fissore, J.B. Cibelli, J.M. Robl and R.T. Duby.........--ee00+--191 IN VITRO SURVIVAL OF MICROMANIPULATED RABBIT EMBRYOS FROZEN IN TWO DIFFERENT CRYOPROTECTANTS R. de Armas, A. Barrios, F.O. Castarndo R. Solamo..........+. e TTT T eter ATTACHMENT OF THE FOLLICULAR CUMULUS-OOCYTE COMPLEX IN SWINE COMPARED TO OTHER FARM SPECIES M.R. Del Campo, C.H. Del Campo, R.J. Mapletoft and O.J. Ginther........... COMPARISON OF FOLLICULAR DEVELOPMENT AND ENDOCRINE PROFILES IN HEIFERS AND MATURE COWS DURING A SYNCHRONIZED ESTROUS CYCLE AND SUPEROVULATION D.M. Desaulniers. J.G. Lussier. A.K. Goff. D. Bousquet and L.A. Guilbault TRANSFORMING GROWTH FACTOR-alpha IN UTERINE LUMINAL FLUID OF PREGNANT AND CYCLING GILTS FROM DAY 10 TO 18 J.R. Diehl, D.M. Henricks and S.L. Gray.......seeeeeeeees eesesees wevTretet oosecekee ISOLATION OF GERM CELLS FROM RABBIT FETAL GONADS d.Ding, A.C. Hahmel and K.d. Betterridgge..ccccccccccccccccccccccccccccs IN VITRO SURVIVAL OF IN VITRO PRODUCED (IVP) BOVINE EMBRYOS FROZEN OR VITRIFIED BY TECHNIQUES SUITABLE FOR DIRECT TRANSFER A. Dinnyes, C. Carolan. P. Lonergan. L. Solti. A. Massip and P. Mermil lod EFFECT OF MONOSACCHARIDES ON THE INTERACTION OF BQUINE SPERMATOZOA WITH OVIDUCTAL EPITHELIAL CELLS IN VITRO 1. Dobrinski. B.A. Ball and P.G.A. Thomas Theriogenology EFFECT OF CYTOSKELETAL STABILIZATION ON THE DEVELOPMENT OF BOVINE EMBRYOS CRYOPRESERVED BY VITRIFICATION J.R. Dobrinsky, E.W. Overstrom. R.T. Duby. L.A. Johnson. P. Daffy, d.F. Rocke and U.P. Boland cccececcocccscccccconscccceoscccecosocosoesolt EFFECT OF FREQUENCY OF FOLLICLE ASPIRATION ON SUBSEQUENT SUPEROVULATORY RESPONSE IN CATTLE D.F. Dolman, P.J. Broadbent, R.G. Watt and L.C. Higgins........ccccccecccecsecess200 COMBINED ACTIVATION TREATMENTS OF RECIPIENT CYTOPLASTS PRIOR TO NUCLEAR TRANSFER IMPROVES DEVELOPMENT OF CLONED EMBRYOS CYTOLOGICAL CHARACTERIZATION OF MATURATION AND FERTILIZATION IN PREPUBERTAL CALF OOCYTES R.T. Duby, P. Damiani, C.R. Looney, C.R. Long, J.J. Balise and J.M. Robl.........202 OVARIAN STIMULATION PROTOCOLS FOR OOCYTE COLLECTION AND IN VITRO EMBRYO PRODUCTION FROM 8 TO 9 WEEK OLD LAMBS C.R. Ear}, B.J. Irvine, J.M. Kelly, J.P. Rowe and D.T. Armstrong.........+++++++-203 FIRST AND SECOND CYCLE NUCLEAR TRANSFER IN CATTLE: COMPARISON OF EFFICIENCY IN EMBRYO DEVELOPMENT AND ONGOING PREGNANCIES F.J. Ectors, A. Delval, L.C. Smith, K. Touati. J-F. Beckers and F. Ectors........204 DURATION OF G2-PHASE AND ONSET OF M-PHASE DURING THE FIRST CELL CYCLE OF THE BOVINE EMBRYO IS DEPENDENT ON BULL IN VIVO FERTILITY LX. Eid and dod. Parrighscccccccccccccccccccscccccccecccccosecccoececceceseooocenee THE EFFECT OF SELENIUM AND VITAMIN E ADDITION ON CLEVAGE RATE OF IVM/IVF BOVINE OOCYTES Y.M. Elhassan and R.W. Wright, Jr....ecccceccccees Covccccesesccoesoocseocecesooocetee CHANGES IN PHOSPHORYLATED SPERM HEAD PROTEINS DURING CAPACITATION. THE ACROSOME REACTION AND BGG PENETRATION M.S. Ellis, C.R. Long and J.M. Robl..........+. ; EARLY FETAL DEVELOPMENT AND TRANSGENE INTEGRATION AFTER PRONUCLEAR INJECTION AND TRANSFER OF MULTIPLE EMBRYOS TO RECIPIENT CATTLE W.H. Eyestone. R.L. Krisher and J. Gibbons.......cccccccccccccccccccscccccsscesseLsU E BOVINE OOCYTE SIZE IN RELATIONSHIP TO FOLLICULAR DIAMETER, MATURATIONAL COMPETENCE AND RNA SYNTHESIS T. Pair, P. Byttel, and Te. Greve ccccccccccccccccccccccesccececoocoocccccocecsceemee COMPARISON OF MENEZO B2 AND TOM-199 MEDIA FOR IN VITRO PRODUCTION OF BOVINE BLASTOCYSTS C.E. Farin, J.F. Basler, amd K.S. Martuse ccccccccccccccccccccccecccccccsces IN VITRO FERTILIZATION OF RACOON DOG (Nyctereutes procynoides Gray) H.L. Feng, X.H. Weng and R.C. Zhang....ccccccccccsccccccccecs SEMEN DILUTION FOR ASSESSMENT OF BOAR EJACULATE QUALITY IN PIG IVM AND IVF SYTEMS G.R. Foxcroft. X. Xu, P.C. Seth, D.S. Harbison and A. P. Cheung........seeeeeeee+212 EFFECTS OF ORGANIC OSMOLYTES IN MATURATION MEDUIM ON INTRACELLULAR GLUTATHIONE CONTENT OF PORCINE OOCYTES H. Funahashi. N.-H. Kim. L.R. Abeydeera and B.N. Day.....ccccccececcccccecesece eotid GnRH INCREASES CLEAVAGE RATES IN BOVINE OOCYTES FERTILIZED IN VITRO RX. Punstemn and G.B. Seidel, Precccccvcccccccccccccecoccccccscccoeses covcccsn4 BOVINE NUCLEAR TRANSFER: BIOCHEMICAL CHARACTERIZATION OF THE RECIPIENT OOCYTE CYTOPLASM L. Gall, T. Dedieu, P. Chesne, Y. Heyman and J.P. Renmard........ ecccceccooccane GRANULOSA CELL SUPPLEMENTATION DURING OOCYTE MATURATION IMPROVES THE DEVELOPMENT OF IVM-IVE BOVINE OOCYTES CULTURED IN THE SHEEP OVIDUCT Cy. Galli. amd G. LazzaPi.ccccccccccccccccccccccccccccccccccccccccesscesccese EFFECTS OF PROGESTERONE TREATMENT ON OVARIAN FUNCTION IN PREPUBERTAL HEIFERS A. Garcia. A.P. Beard. A.C.O. Evans. and N.C. Rawlings........- LIVE BIRTH OF HYBRID (C. musimon x Q. aries) LAMBS FOLLOWING INTRAUTERINE INSEMINATION IN DOMESTIC SHEEP WITH MOUFLON SEMEN OBTAINED 40 HOURS POSTMORTEM Jd. Garde. S. Perez. M.J. Aguado, E. Ayllon. D. Garrido and \. Montoro... 1436 Theriogenology EFFECTS OF RECOMBINANT PORCINE FSH ON GERMINAL VESICLE BREAK DOWN OF PORCINE OOCYTES AND PROGESTERONE SECRETION BY GRANULOSA CELLS CULTURED IN VITRO M. Geshi, M. Sakaguchi, M. Yonai. K. Okuda, Y. Kato. K. Tomizawa, I. Sato, T. Ihara, S. Ueda, J. Mori and T. Nagai......cecceeeceeeees2l9 INNER CELL MASS CELLS IN BLASTOCYSTS DERIVED FROM NUCLEAR TRANSFER OF EITHER BLASTOMERES OR RABBIT EMBRYONIC STEM-LIKE CELLS J.R. Giles, F. Du, K.H. Graves, R.W. Moreadith, X. Yang and R.H. Foote...........220 PRETREATMENT WITH RECOMBINANT BOVINE SOMATOTROPIN ENHANCES THE SUPEROVULATORY RESPONSE TO FSH IN HEIFERS J.G. Gong, I. Wilmut, T.A. Bramley and R. Webb............ VIABILITY CHARACTERISTICS OF KILLED BOVINE SPERMATOZOA USED FOR INTRACYTOPLASMIC SPERM INJECTION Be Gote, B. Debian cok FT. Wea sv cccncpvcccccccosesccadcccccsevccesvcecececcetse ULTRASOUND-GUIDED TRANSVAGINAL OOCYTE RECOVERY FROM FSH-TREATED GOATS FOR IVF K.J. Graff, M. Meintjes, J.B. Paul. V.W. Dyer, B.S. Demmistem, C. Ziemek amd BoA. Godcec ccccccccccccccccvccccccocccccccccccccccehee BINDING OF SPERMATOZOA TO BOVINE OOCYTES AND THEIR PENETRATION RATES IN A HOMOLOGOUS IVF SYSTEM DB Gramie, 3. Bramm amb BR. Bteblaccceccccccccccccsccccccedsceescevccecose cece A NEW METHOD FOR COLLECTION OF OVIDUCT FLUID IN CATTLE T. Greve and Th. Host Hamsen.....ccccscccccscceccecs PPTTTTTTTI TITT ITTTT ITTTT. PREOVULATORY OOCYTE MATURATION AND FOLLICULAR STEROID CONCENTRATIONS IN THE MARE C. Grondahl, P. Hyttel, P. Gotfredsen and T. Greve....cccccccccccccescccccsseceeete& CYSTEAMINE ENHANCES IN VITRO DEVELOPMENT OF IVM-IVF PORCINE OOCYTES C.G. Grupen, H. Nagashima and M.B. Nottle......ccescccccccccccccccees escaevesceneme ALTERATION IN MICELLE SIZE OF MILK FROM TRANSGENIC MICE EXPRESSING A BOVINE k-CASEIN GENE A. Gutierrez.E.A. Maga,H.M. Meade,J.F. Medrano,.G.B. Anderson and J.D. Murray.....228 PRONUCLEAR INJECTION OF MOPS-BUFFERED MEDIUM DOES NOT AFFECT BOVINE EMBRYO DEVELOPMENT cde BAEEEMn ccc ccccsccccenecoccccecceessccccceenceeseocoesessccosescoceceesoooneD EFFECT OF METHYLATED XANTHINE ON SPERM PENETRATION OF BOVINE OOCYTES MATURED IX VITRO A. Hamawaki, S. Hamano and M. Kuwayama......... TTT TTTITTTT TTTT TTTT TT TTT T EFFECT OF HUMAN LEUKEMIA INHIBITORY FACTOR ON IN VITRO DEVELOPMENT OF IVF-DERIVED BOVINE MORULAE Y.M. Han, E.S. Lee, T. Mogoe, K.K. Lee and Y. Fukuii.....cccccccccccsceccsces NON-SURGICAL TRANSFER OF PORCINE BLASTOCYSTS W. Hazeleger, P. Jenneskens and B.K COMPARISON OF CO-CULTURE AND CONDITIONED MEDIUM ON EXPANSION "AND "HATCHING OF IN VITRO-DERIVED BOVINE BLASTOCYSTS J.J. Hernandez-Ledezma, C. Villanueva, J.D. Sikes and R.M. Roberts.......++++++++233 GESTATIONAL PROFILES FOLLOWING TRANSFER OF CLONED BOVINE BLASTOCYSTS DEVELOPED IN VITRO Y. Heyman, S. Camous, P. Chesne. J. Marchal and J.P. Remard......cseeeeseeeeeeeee2a4 \ SIMPLE METHOD OF TRANSVAGINAL FOLLICLE ASPIRATION BAR. Bil cccccccccesces eseneeenee suepeseoesaccecescenes VIABILITY OF IMMATURE HORSE OOCYTES CRYOPRESERVED | BY VITRIFICATION S. Mochi, T. Fujimoto amd N. Qguri.ccccccccccccccccesccccs oceee TOXIC EFFECT OF ETHYLENE OXIDE RESIDUES ON IN VITRO PRODUCTION OF BOVINE EMBRYOS GR. Bolpeak, S. Wag ae Vo Lite ccccccccccccccccccccncccccccccccocccvccccccccceeset TRANSCRIPTION AND DEVELOPMENT OF INTRANUCLEAR BODIES AND PARTICLES DURING THE 2-CELL STAGE IN CATTLE P. Hyttel, D. Viuff and B. Avery..... a CO-CULTURE OF EMBRYONIC STEM (ES) CELLS WITH MOUSE EMBRYOS: EFFECTS OF PHYTOHEMAGGLUTININ (PHA) ON ADHERENCE OF ES CELLS K.S. Jackson. E.D. Hunt. V.L. Jarrell. L.A. Rund and M.B. Wheeler... COMPARATIVE EFFICIENCY OF THREE DIFFERENT COLLECTION TECHNIQUES FOR OOCYTE RETRIEVAL IN BUFFALOES G.C. Jain. G.K. Das. V.S. Solanki and V.N. Tripathi Theriogenology DETECTION OF GENE EXPRESSION IN SINGLE PREIMPLANTATION BOVINE EMBRYOS BY REVERSE TRANSCRIPTION-T7 RNA POLYMERASE-DEPENDENT AMPLIFICATION(RT-TRDA) K.L. Jones, J.A. Piedrahita, R.H. Finnel] and M.E. Westhusin.........-eeeeeeeeeee24! DETECTION OF BOVINE FETAL DNA BY POLYMERASE CHAIN REACTION FOR PRENATAL SEXING H. Kadokawa, M. Minezawa, N. Takusari, H. Takahashi, O. Sasaki and T. Kariya.....242 THE EFFECTS OF FSH AND LH ON BGI, IL-2 AND GONADOTROPHIN BINDING BY GRANULOSA CELLS IN CATTLE W. Kamitz, R. Pohland and F. Becker. cocccccccccccccccccceccccccccccccccscceocccesdee EFFECT OF PERCOLL FOLLGWING SPERM SEPARATION ON IN VITRO FERTILIZATION OF BOVINE OOCYTES C.L. Keefer and A.M. Paprocki.cccccccccccccccccccccccccccccccccccccccccoccecccccotne EFFECT OF FSH TYPE AND NUMBER OF INJECTIONS ON PERIPHERAL FSH CONCENTRATIONS, FOLLICLE NUMBERS AND EMBRYO YIELD IN HEIFERS P. Kelly, P. Duffy, A. Baguisi, J.R. Dobrinsky, E.W. Overstrom, R.T. Duby, J.F.Roche and M.P. Bolamd...ccccccccccccccccccccccccccccccccccccscccsethd AGE- AND PARITY-DEPENDENT DIFFERENCES IN OVARIAN ACTIVITY AND OOCYTE MATURITY IN THE AFRICAN ELEPHANT (Loxodonta africana) A. Kidson, N.M. Loskutoff. C. Raath, C.A. Wood, K.R. Williams, J.O. van Schalkwyk, W.K. Dyche, D.M. Barry and P. Bartels......csecececececeeeee2e4 6 CYTOPLASMIC MATURATION OF PIG FOLLICULAR OOCYTES ARRESTED AT METAPHASE-I K. Kilsohi, T. Magni and Y. Usable cccccccccccccccccccescccocccccccecocoesoceccssonet EXPRESSION PATTERNS OF mRNA FOR MEMBERS OF THE ALKALINE PHOSPHATASE FAMILY IN EMBRYONIC STEM AND PRIMORDIAL GERM CELLS OF MOUSE J.H. Kim, D.B. Koo, H.T. Lee and K.S. Chung..... ecrcccbcccccccececeococceceenee MICROTUBULE AND MICROFILAMENT DYNAMICS IN PORCINE OOCYTES DURING IN VITRO MATURATION N.H. Kim. R.S. Prather. G. Schatten and B.N.Day....ccscccsccccccccecccccsccccesssth® SWAMP BUFFALO OOCYTES FROM TRANSVAGINAL ULTRASOUND-GUIDED ASPIRATION FERTILIZED AND CO-CULTURED IN VITRO WITH BOVINE OVIDUCTAL EPITHELIAL CELLS Y. Kitiyanant, C. Tocharus, M. Areekijseree and K. Pavasuthipaisit...............250 DEVELOPMENT AND EVALUATION OF A TREATMENT REGIMEN FOR SUPEROVULATION AND EMBRYO SURVIVAL IN PIGS R.V. Knaondx D. R. Zimmerman...........+- cooseckee FOLLICULAR STATUS AND SUPEROVULATION IN CATTLE: A FIELD TRIAL H. Kohram, D. Bousquet, J. Durocher and L.A. Guilbault........ccecceeecccceeececedve PRESENCE OF GRANULOSA CELLS DURING OOCYTE MATURATION IMPROVED IN VITRO DEVELOPMENT OF IVM-IVF BOVINE OOCYTES COLLECTED BY ULTRASOUND-GUIDED TRANSVAGINAL ASPIRATION M. Konishi, Y. Aoyagi. T. Takedomi, H. Itakura and T. Wada.......sseseeeeeeeeseee2dd CASEIN PHOSPHOPEPTIDES ENHANCE IN VITRO FERTILIZATION WITH SEMEN OF LOW PENETRABILITY U. Kreysing, T. Nagai and H. Niemamn.....ccccccccccccccccccscccccccccccccccccccccsnt TRANSGENE EXPRESSION IN IVM/IVF-DERIVED BOVINE EMBRYOS AFTER DELAY OF MATURATION WITH 6 DIMETHYLAMINOPURINE H.M. Kubisch, M.A. Larson, F.L. Barnes, J.D. Sikes and R.M. Roberts.....+++++++++255 THE RESPONSE OF BOVINE GRANULOSA CELLS TO GONADOTROPHINS IN AN IN VITRO CULTURE SYSTEM M. Kuran, P.J. Broadbaned nJ.St.M . Hutchinson. eccccevccccces esccocsescecae VITRIFICATION OF IVMFC BOVINE EMBRYOS AT VARIOUS DEVELOPMENTAL STAGES AND OF DIFFERENT QUALITY M. Kuwayama......ceesseeeseeess a CRYOPRESERVATION OF BOVINE EMBRYOS AND DEMI-EMBRYOS USING ETHYLENE GLYCOL FOR DIRECT TRANSFER AFTER THAWING B. LEMBBccccccccess nthe deASn Ob eeeeenséeewsnewnsdsnesseiaseeeennee FACTORS AFFECTING OVUM PICK UP IN CATTLE L.M.T.C. Lansbergen, A.M. van Wagtendonk-de Leeuw, J.H.G. den Daas, L. de Ruigh. G. van der Streek. J.M.C. Reinders. M. Aarts and J. Rodewij k BINDING OF OVIDUCTAL. SPECIFIC PROTEINS TO THE MEMBRANE OF BOVINE SPERMATOZOA S. Lapointe and M.A. Sirard..........-. THE EFFICIENCY OF DIFFERENT GONADOTROPHIN PREPARATIONS ON THE SUPEROVULATORY RESPONSES OF HOLSTEIN DONORS C. Larocca, A. Fernandez, A.F. Gonzalez and A.A. Carbo....seeeececcccceecscecees 1438 Theriogenology COMPARATIVE EXPRESSION PATTERNS OF TWO TRANSGENES IN MURINE, PORCINE AND BOVINE EMBRYOS M.A. Larson, H.M. Kubisch, H. Funahashi, J.J. Hernandez-Ledezma. B.N. Day and R.M. Roberts.....ccccccccccccsccccccccseceseslbe SEX SHIFT IN CALVES DERIVED FROM IVM-IVF EMBRYOS CULTURED IN THE SHEEP OVIDUCT VERSUS CALVES PRODUCED BY CONVENTIONAL SUPEROVULATION AND EMBRYO TRANSFER G. Lazzari, R. Landriscina, R. Duchi and C. Galli........... eSeeccecossececones ANALYSIS OF CELL CYCLES OF BOVINE GRANULOSA CELLS DURING FOLLICULAR MATURATION BY FLOW CYTOMETER H.T. Lee, H.J. Kim, D.H. Kim, B.H. Chung and K.S. Chung........cecccccscccccscees2b4 CHILLING AND FREEZING SENSITIVITY OF “REASSEMBLED” IN VITRO-DERIVED BOVINE EMBRYOS S.P. Lethe, SM. Pollard: amd An Martindsccccccccccccveccevccccessescccescscccccsotee PREGNANCY RATE FOLLOWING TRANSFER OF IVF BOVINE EMBRYOS AT DIFFERENT DEVELOPMENTAL STAGES Z.J. Ling, D.S. Shi. H.M. Huang, Y.M. Wei. R.M. Jiang and K.H. Lu........2+++++++266 THE IMPORTANCE OF CUMULUS CELLS ON THE IN VITRO PRODUCTION OF BOVINE OOCYTES Y. Liu, G.R. Holyoak, S. Wang and T.D. Bunch.......cccsccccccscccccccseees EFFECTS OF 02 CONCENTRATIONS AND SUPEROXIDE DISMUTASE ON THE DEVELOPMENT OF IVM/IVF BOVINE EMBRYOS IN KSOM WITH 2.5aM HEPES Be BOO BD Bele. Fab Ri cccccnscdccveccccccccccccccscceceseessesaseneenoocecceeeeeotee USE OF PREPUBERAL HEIFERS AS OOCYTE DONORS FOR IVF: EFFECT OF AGE AND GONADOTROPHIN TREATMENT C.R. Looney, P. Damiani. B.R. Lindsey, C.R. Long, C.L. Gonseth, D.L. Johnson and R.T. Duby......... OTT TTITTITTT TTITTT T T EFFECTS OF FOLLICULAR DOMINANCE AND DIFFERENT GONADOTROPIN PREPARATIONS ON THE SUPEROVULATORY RESPONSE IN COWS J.G. Lussier, P. Lamothe and X. Pacholek ACTIVATION OF THE G PROTEIN IN PORCINE OOCYTES Z. Machaty, M.A. Mayes and R.S. Prather....... Ceeceseveccooccesceoereessocoeccccents THE KINETICS OF CHILLING SENSITIVITY OF BOVINE OOCYTES COOLED TO NON-PHYSIOLOGICAL TEMPERATURES A. Martino, J.W. Pollard, A. Nakagawa and S.P. Leibo.....cccccccccccccececscessseria ENDOCRINE AND BEHAVIOURAL EVENTS OF ESTROUS CYCLICITY AND SYNCHRONIZATION IN WOOD BISON (Bison bison athabascae) D.M. Matsuda-Motomura, C.J. Gartley, A.C. Bellem, R.M. Liptrap, V. Madison and K.L. Goodrowe.......ccccccecccccccccccsscsssesesssesrid ULTRA-RAPID FREEZING OF IN VITRO PRODUCED BOVINE EMBRYOS K. Matsuoka, S. Sakata, K. Ichino, S. Shimaya, T. Katagihara and T. Suzuki.......274 USE OF LINEAR ULTRASOUND TRANSDUCER FOR COMMERCIAL APPLICATION OF TRANSVAGINAL OOCYTE RECOVERY L. Matthews, H. Petersen and K. Van Beek........... RELATIONSHIP BETWEEN PRE-OVULATORY FEED INTAKE. PROGESTERONE " PRIMING AND THE SUBSEQUENT IN VITRO DEVELOPMENT OF OVA COLLECTED FROM SUPEROVULATED EWES T.G. McEvoy, J.J. Robinson. R.P. Aitken. P.A. Findlay and I.S. Robertson.. DOMESTIC COWS CAN CONTRIBUTE TO SPECIES PRESERVATION EFFORTS J.A. McHugh, R.L. Monson, M.L. Leibfried-Rutledge and J.J. Rutledge..........+++-277 EVIDENCE FOR A HIGH FREQUENCY OF EMBRYO MIGRATION IN CATTLE AFTER UNI-LATERAL TWIN EMBRYO TRANSFER W.Il. McMilalnda An.J . Peterson.......... ocecceeses " IN VITRO FERTILIZATION OF IN VITRO MATURED OOCYTES RECOVERED FROM FREE RANGING ZEBRA IN SOUTH AFRICA M. Meintjes, P. Bartels. C. Bezuidenhout. D.S. Visser, J. Meintjes, N.M. Loskutoff, F. le R. Fourie, D.M. Barry and R.A. Godke...ccccccccccceccceseeerl® EFFECT OF CULTURE MEDIA AND ©O2 CONCENTRATION ON EMBRYO DEVELOPMENT IN A BOVINE EMBRYO IN VITRO PRODUCTION SYSTEM J.S. Merton, J.A.W. van Dillen, J.f. Hasler and J.H.G. den Daas.......eeeeeeeeeeL eR U MORPHOLOGICAL CHANGES NOTED IN CORPORA LUTEA POST-PROGESTAGEN AND/OR OVULATION INDUCTION THERAPY IN BOBCATS (Felis rufus) D.L. Miller. B.D. Leopold and S.J. Waldhalm Theriogenology 1439 GLUTATHIONE CONCENTRATION DURING MATURATION AND FERTILIZATION IN BOVINE OOCYTES M. Miyamura, M. Yoshida, S. Hamano and M. Kuwuayama........scccccccsccccccccceeeslBe NUCLEAR TRANSFER USING GONIA FROM MALE RABBIT FETUSES A. Moens, S. Chastant, P. Chesne, K.J. Betteridge and J.P. Renard.........+++++++283 EFFOEF HCORMTONE S, SERUM SOUARND CCUELTU RE SYSTOEN MTH E IVM AND IVF OF PREPUBERT: GOAT OOCYTES AND SUBSEQUENT EMBRYO DEVELOPMENT T. Mogas, M.D. Izquierdo, M.J. Palomo and M.T. Paramio......cccsccccccececececesstOh SUCCESSFUL CRYOPRESERVATION OF PORCINE EARLY CLEAVAGE STAGE EMBRYOS FOLLOWING REMOVAL OF CYTOPLASMIC LIPID H. Nagashima, N. Kashiwazaki, R.J. Ashman and M.B. Nottle.....ccecceccccecccceeeelBd STAGE OF NUCLEAR MATURATION OF BOVINE OOCYTES AT INSEMINATION INFLUENCES SPERM PENETRATION AND ZYGOTE DEVELOPMENT A. Nakagawa, A. Martino, J.W. Pollard and S.P. Leibo.....ccccccescccccccccceseeee2s0 0 ULTRASONOGRAPHY OF OVULATION IN RELATION TO FIRST CLEAVAGE IN hOG-TREATED SOWS A.K. Nissen, M. Schmidt, P. Hyttel and T. Greve.....cccccccccccccccccccssceceseeslOl PYRUVATE REVISITED: A NON-METABOLIC ROLE FOR PYRUVATE IN PREIMPLANTATION EMBRYO peveLopment J.V. O' Fallen and BW. Wright, Weeccecccccccocccecovcesccssseceseceosesesscessesene VITAMIN E IMPROVES DEVELOPMENT OF BOVINE EMBRYOS PRODUCED IN VITRO S.E. Giaem and G.B. Seidel, Wecccccccscescscccecevetecceseeccesccececetocessooscnee REFRIGERATION OF BOVINE OOCYTES IN HEAT-STABLE PLANT PROTEINS A. Palasz. L. Gusta, H. Gustafsson, B. Larsson and H. Rodriguez-Martinez.........290 EFFECT OF GROWTH FACTORS IGFI,. TGF-alpha. BGF AND PDGF ON DEVELOPMENT OF IN VITRO PRODUCED BOVINE BLASTOCYSTS G.A. Palma and G. Brem. 00000000000 0006006e0ee00eeonsdéseeseeennncnen EFFECT OF HEPARIN AND SPERM CONCENTRATION ON IVF OF PREPUBERTAL GOAT OOCYTES M.J. Palomo, T. Mogas. M.D. Izquierdo and M.T. Paramio.......ccccccccccccccescesedde THE EFFECT OF VITRIFICATION SOLUTION. BQUILIBRATION TIME, AND DIRECT DILUTION METHOD ON SURVIVABILITY OF BQUILIBRATED OR VITRIFIED BOVINE IN VITRO MATURED OOCYTES K. Papis, B. Avery, P. Holm, H. Callesen and T. Greve....ccccccccccccccscsesesseeadd & GONADOTROPIN STIMULATION OF CATTLE DONORS AT ESTRUS FOR TRANSVAGINAL OOCYTE COLLECTION J.B. Paul, C.R. Looney, B.R. Lindsay and R.A. Godke.....cecscccccccccccccseccseesldd REPEATED TRANSVAGINAL FOLLICULAR ASPIRATION IN SWAMP BUFFALO K. Pavasuthipaisit, R.G. Holyoak, C. Tocharus and Y. Kitiyamant.......seeeeeseeeee299 QUANTITATIVE ANALYSIS OF GENE EXPRESSION CHANGES DURING DIFFERENTIATION OF MURINE EMBRYONIC STEM (ES) CELLS J.A. Piedrahita, A.D. Petrescu. M.A. Piedrahita and C-K. Lee......eceeseceeceeee2e9 6 IN VITRO FERTILIZING CHARACTERISTICS OF BOVINE SEMEN WITH MULTIPLE NUCLEAR V. R. Pilip, M.R. Del Campo and R.J. Mapletoft......cccccccccccccccccccsccccseeseeseedl DEVELOPMENT OF SINGLE BLASTOMERES FROM 4-CELL STAGE EMBRYOS AFTER AGGREGATION WITH PARTHENOGENONES IN MICE A. Pinyopummin, Y. Takahashi. M. Hishinuma and IH. Kanagawa........ceeseseeseseeee2e9 8 MINIMUM ENERGY SUBSTRATE REQUIREMENTS FOR EARLY CLEAVAGE STAGES OF BOVINE EMBRYO DEVELOPMENT IN VITRO T. Pinyopummintr and B.D. Bavister.... $680606b0000000060600000600080 ISOLATION AND LONG-TERM CULTURE OF MINK AND BOVINE EMBRYONIC STEM-LIKE CELLS 1.A. Polejaeva, K.L. White, L.C. Ellis and W.A. Reed........ vebencessessboosnessenee IN VITRO PRODUCTION OF WAPITI AND RED DEER (Cervus elaphus) EMBRYOS J.W. Pollard, M.J. Bringans and B. Buckrel]l.......sceccscccccccccesececcccccesessdOl IN VITRO AND IN VIVO DEVELOPMENT OF DOMESTIC CAT OOCYTES FOLLOWING INTRACYTOPLASMIC SPERM INJECTION OR SUBZONAL INSEMINATION C.E. Pope. C.A. Johnson, M.A. McKae. G.L. Keller and B.L. Dresser... ROLES OF TRIFLUOPERAZINE ANT) VERAPAMIL IN OOOCYTE MATURATION AND CUMULUS EXPANSION OF BOVINE CUMULUS-OOCYTE COMPLEXES - Qin. Q.¥. Sun. H.L. Feng. G.Y¥. Liu and Q.Z. Yang....sccccccccccccccccccecesed03 RATIO AND NUMBER OF INNER CELL MASS AND TROPHOBLAST CELLS OF IN VITRO AND IN VIVO PRODUCED PORCINE EMBRYOS D. Rath, H. Niemann, T. Tao and M. Boer jan......cccecccccccccccccccccecceceseeseds04 d 1440 Theriogenology THE CONTENT OF IGF1 AND IGF BINDING PROTEINS IN PORCINE FOLLICULAR FLUID AT DIFFERENT STAGES OF THE ESTROUS CYCLE K. Raun, B. Sehested Hansen, J. Sondergard-Andersen and M. Schmidt...............305 FROM EMBRYO TO CALF AFTER EMBRYO TRANSFER OF IN VITRO PRODUCED BOVINE EMBRYOS J.M.C. Reinders, Y.A. Wurth and Th.A.M. Kruip COONS OOREOCeDESCNRE+ eeeess cDee ee EFFICIENCY OF DERIVATION OF PLURIPOTENT BOVINE EPIBLAST CELLS FROM IVM-IVF PRODUCED AND IN VIVO COLLECTED BLASTOCYSTS C.E. Rexroad, Jr., A.M. Powell and N. Talbot....cccccccccccccsccccccccesesesesesdOl EFFECTS OF ANTIBIOTICS ON DEVELOPMENTAL CAPACITY OF BOVINE EMBRYOS K.P. Riddell, D.A. Stringfellow, B.W. Gray and M.G. Riddell, Jr....ceeeeeeeeee+s3e0 8 SNOW LEOPARD (Panthera uncia) SPERM LONGEVITY IN VITRO IS NOT INFLUENCED BY PROTEIN OR ENERGY SOURCE SUPPLEMENTBSU T IS AFFECTEDB Y BUFFER SOURCE T.L. Roth, W.F. Swanson, and D.E. Wildt..........eeeeeees TTT TTT TTT TTT TT TT EFFECT OF ESTRADIOL SUBSEQUENT TO INDUCED LUTEOLYSIS ON DEVELOPMENT OF THE OVULATORY FOLLICLE AND INTERVAL TO ESTRUS AND OVULATION D.P. Ryan, S. Snijders, A. Asarts and K.J. O'Farrell....ccccccccecccccsccceeesessdlO VIABILITY OF BOVINE BLASTOCYSTS OBTAINED AFTER 7, 8 OR 9 DAYS OF CULTURE FOLLOWING VITRIFICATION AND ONE-STEP REHYDRATION S. Saha, R. Rajamahendran, A. Boediono, S. Cece and T. Suzuki.........seeeeeeeeee3ll DEVELOPMENT OF BOVINE IVF EMBRYOS IN A CR-1 CULTURE MEDIUM SUPPLEMENTED WITH ALBUMIN OR SERUM D.F. Salamone, A. Valdez and J.B. Baranao.. CCHODECSHCSSSCCCCCeCeeOceCesooehe SYNCHRONIZATION OF CELL DIVISIONS IN BOVINE EMBRYOS PRODUCED IN VITRO S. Samake and L.C. Smith........cceceeeeeess Coos oe SeSoesesesroosecoeocccececesooceesae EFFECT OF STORAGE PERIOD ON 3H-HEPARIN BINDING TO BOAR SPERMATOZOA J. Sanchez-Prieto. M.J. Illera, P.L. Lorenzo, J. Gonzalez and L. Orensanz........314 DEVELOPMENTAL COMPETENCE OF MOUSE EMBRYOS FOLLOWING ZONA DRILLING USING A NON-CONTACT HO:YAG LASER SYSTEM M.C. Schiewe,J.Y. Neev.N.L. Hazeleger.Y. Tadir.M.W. Berns and J.P. Balmaceda.....315 THE EFFECT OF RBGUMATE ON THE SYNCHRONIZATION OF THE ESTOUS CYCLE IN MICROPIGS EFFECTS OF THE 14:20 ROBERTSONIAN TRANSLOCATION ON FERTILITY IN SUPEROVULATED CATTLE S.M. Schmutz, J.S. Moker. V. Pawlyshyn. B. Haugen, L. Heinrich and M. Munroe.....317 FERTILITY OF FROZEN BOAR SPERM WHEN DEPOSITED SURGICALLY IN THE OVIDUCT RA. Soheambeck and B.A. DiGidiccccccoccccvcccccccccccssceseccccccccsccececceccccdse PREPARATION OF BOVINE SPERM FOR IN VITRO FERTILIZATION BY SWIM-UP OR CENTRIFUGATION THROUGH PERCOLL OR BSA G.E. Seidel, S.D. Leipold and H. Shawki.........eeeeeeeseess CRYOPRESERVATION OF IN VITRO-DERIVED BOVINE EMBRYOS PRODUCED IN A SERUM- FREE CULTURE SYSTEM M.E. Semple, K.J. Betteridge and S.P. Leibo....... TT TTT TT TTT TT TTT TTT TTT TTT THE FIRST SUCCESSFUL TRANSFER OF FROZEN EMBRYOS DERIVED FROM IVMFC OF BOVINE OOCYTES IN HUNGARY J. Seregi, S. Cseh and A Treuer......... PPVT TTTTITT IITT TTTIT TTTT TTTT IN VITRO PRODUCTION OF AFRICAN BUFFALO (Syncerus caffer) EMBRYOS DERIVED FROM FOLLICULAR OOCYTES AND EPIDIDYMAL SPERM D.G. Shaw, A. Kidson. J.O. van Schalkwyk,. P. Bartels, N.M. Loskutoff. C. Bezuidenhout, P. Rogers, D.M. Barry and A.W. Lishman........ EFFECTS OF GLYCINE AND ALANINE ON THE IN VITRO DEVELOPMENT OF EARLY BOVINE. EMBRYOS DERIVED FROM IVM/IVF D.S. Shi, $.d. Tam, FL. Mang amd Ku. Like cccccccccccccccccccccccccecs ULTRARAPID REFREEZING OF BIOPSIED MOUSE EMBRYOS S.T. Shin, J.H. Lim. M.J. Kang, C.S. Lee. Y.M. Ham and K.K. Lee......eseeeeeeeeeed24 USE OF GnRH TO SYNCHRONIZE OVULATION IN HOLSTEIN COWS AND HEIFERS TREATED WITH GnRH AND PROSTAGLANDIN R.W. Silcox. K.L. Powell. J.R. Pursley and M.C. Wiltbank.....

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