The Steroid/Thyroid Hormone Receptor Family and Gene Regulation: Proceedings of the 2nd International CBT Symposium Stockholm, Sweden, November 4–5, 1988

360 Pages·1989·11.426 MB·English

by  Ronald M. Evans (auth.), Prof. Jan Carlstedt-Duke, Prof. Håkan Eriksson, Prof. Jan-Åke Gustafsson (eds.)

Birkhauser Congress Reports
Life Sciences, Vol. 4
The Steroid/Thyroid
Horm.one Receptor Fam.ily
and Gene Regulation
Prooeedings of the 2nd International CBT Symposium
Stookholm, Sweden, November 4-5,1988
Edited by
J. Carlstedt-Duke
H. Eriksson
o
J.-A Gustafsson
1989 Springer Basel AG
Editors' addresses:
Prof. Jan Carlstedt-Duke Prof. HAkan Eriksson
Prof. Jan- Ake Gustafsson Reproductive Endocrinology
Dept. of Medical Nutrition Research Unit
Karolinska Institute Karolinska Hospital
Huddinge University Hospital F69 S - 10401 Stockholm
S-14186Huddinge Sweden
Sweden
CIP-Titelaufnahme der Deutschen Bibliothek
The steroidlthyroid hormone receptor family and gene regulation: Stockholm, Sweden, Novem
ber 4-5,19881 ed. by J. Carlstedt-Duke ... - Basel; Boston; Berlin: Birkhauser, 1989
(Proceedings of the ... international CBT symposium; 2) (Birkhauser congress reports, life
sciences; Vol. 4)
ISBN 978-3-0348-5468-9 ISBN 978-3-0348-5466-5 (eBook)
DOI 10.1007/978-3-0348-5466-5
NE: Carlstadt-Duke, Jan (Hrsg.); Center for Biotechnology <Stockholm>: Proceedings of the ... ;
2.GT
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Originally published by Birkh!tuser Verlag in 1989
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ISBN 978-3-0348-5468-9
5
CONTENTS PAGE
Preface ......................................................................................................................... 9
RECEPTOR STRUCfURE
The contributions of the steroid receptor superfamily to development,
physiology and medicine
Ronald M. Evans........................................................................................................... 11
Cooperative interactions of steroid receptors at their target enhancers
Bert W. O'Malley, S.Y. Tsai, M.-J. Tsai, O.M. Conneely, and W.T. Schrader................. 29
The association of the glucocorticoid receptor with Mr 90,000 heat shock
protein and tubulin
Ann-Charlotte Wikstrom, Marc Denis, Gunnar Akner, Oddmund Bakke, and Jan-Ake
Gusta/sson...................................................................................................................... 41
Functional domains of steroid hormone receptors
H. Gronemeyer, V. Kumar, S. Green, M.T. Bocquel. L. Tora, M.E. Meyer. J. Eul.
and P. Chambon............................................................................................................ 55
Thyroid hormone receptor interactions with DNA
T.N. Lavin, M.F. Norman. N. Eberhardt and J.D. Baxter............................................. 69
Structure and intranuclear dynamics of androgen receptors
S. Liao, C. Chang, J. Kokontis. T. Popovich, and R.A. Hiipakka............................... 83
Structural analysis of the glucocorticoid receptor protein
Jan Carlstedt-Duke. Per-Erik Stromstedt, Karin Dahlman, Carol Rae, Anders
Berkenstam. Janet Hapgood. Hans Jornvall and Jan-Ake Gusta/sson....................... 93
Speculations on the role of the 90 kDa heat shock protein in glucocorticoid
receptor transport and function
W.B. Pratt. T. Redmond. E.R. Sanchez. E.H. Bresnick. S. Meshinchi.
and M.J. Welsh............................................................................................................... 109
6
Growth inhibition of CEM cells by glucocorticoids: c-myc down regulation, and
the topology of the glucocorticoid receptor
E. B. Thompson, Y -So Yuh, D. Harbour, J. Ashraf, B. Johnson, and J.M. Harmon.. 127
The vitamin D3 receptor and its chromosomal gene
J.W. Pike, D.P. McDonnell, R.A. Scott, S.A. Kerner, R.A. Kesterson,
and B.W. O'Malley......................................................................................................... 147
The thyroid hormone receptor/c-erbA protein and its viral homologue
P75g·g-v-erbA
Bjorn Vennstrom, Jan Sap, Jackie Schmitt, Douglas Forrest, Alberto Munos, Martin
Zenke, Henk Stunnenberg, and Hartmut Beug............................................................ 161
Characterization of the human androgen receptor
P.W. Faber, G.G.J.M. Kuiper, H.C.J. van Rooij, J.A.G.M. van der Korput, A.O.
Brinkmann, and J. Trapman......................................................................................... 169
Characterization of new members of the steroid receptor super-family
Chawnshang Chang, Lester Lau, Shutsung Liao, and John Kokontis........................... 183
GENE REGULATION BY RECEPTORS:
Reciprocal regulation of PEPCK gene and gene 33 transcription by insulin
D. Granner, D. Chu, C. Davis, and N. Chrapkiewicz...................................................•.. 195
Repression of gene expression by glucocorticoid receptor through interference
with cAMP responsive enhancers
Pamela L. Mellon, and Ingrid E. Akerblom.................................................................... 207
Steroid transactivation at a promoter organized in a specifically-positioned
array of nucIeosomes
T.K. Archer, M.G. Cording ley, V. Marsaud, H. Richard-Foy, and G.L. Hager.............. 221
Glucocorticoid regulated sorting of cell surface glycoproteins: Evidence for a
glucocorticoid regulated trafficking gene
Deborah A. Bravo, Emily J. Platt, Laurie J. Goodman, and Gary L. Firestone.......... 239
Interaction of a steroid hormone receptor with DNA:
Molecular model and kinetic analysis
M. Beato, G. Chalepakis, and M. Schauer.................................................................... 253
RECEPTOR LOCALISATION AND DISTRIBUTION:
Neural gonadal steroid receptors and actions: Chemical anatomy of the
ventromedial hypothalamus in relation to sexual differentiation and sexual
behavior
Bruce S. McEwen, Hector Coirini, Maya Frankfurt, John Gerlach, Allan Johnson,
and Michael Schumacher............................................................................................... 263
7
Stucture, function and cellular distribution of mammalian progesterone
receptors
A. Bailly. A. Guiochon-Manlel. M. Misrahi. H. Loos/elt. M. Alger. J.F. Savouret.
M. Perrot-Applanat. M.T. Vu Hai. M. Rauch. F. Lorenzo. F. Logeat. and E.
Milgrom......................................................................................................................... 271
Cellular localization of estrogen and progestin receptors in the macaque
reproductive system
Robert M. Brenner. Neal B. West. Maryanne C. McClellan. Sheri A. Hild-Petito. and
Richard L. Stou//er........................................................................................................ 281
Do receptor-associated nuclear proteins explain earliest steps of steroid
hormone function?
E.E. Baulieu. N. Binart. F. Cadepond. M.G. Catelli. B. Chambraud. J. Garnier. J.M.
Gasc. G. Groyer-Schweizer. M.E. Oblin. C. Radanyi. G. Redeuilh. J.M. Renoir. and
M. Sabbah....................................................................................................................... 301
LIGAND STRUcruRE:
Steroid molecular structure, receptor binding and hormone action
W.L. Duax and J.F. Gri//in............................................................................................ 319
An analysis of the steroid binding domain of receptors and of ligand structure
and binding affinity
J.P. Raynaud. V. Bissery. C. Gaboriaud. T. Ojasoo. G. Teutsch. and J.P.
Mornon........................................................................................................................... 337
Author Index .............................................................................................................. 367
Subject Index .............................................................................................................. 369
9
PREFACE
The Second International CBT (Center for Biotechnology) Synposium was held in
Stockholm November 4-5 1988 and had the title "The steroid/thyroid hoITIDne
receptor family and gene regulation". This meeting contained contributions
from most leading laboratories within the field of steroid/thyroid hOITIDne
receptors and gave a very exciting perspective on the dynamic development of
this i:ntx>rt.ant research field. Today all known steroid hoITIDne receptors have
been cloned and sequenced and novel members of this supergene family are
constantly being discovered. Sane of these new receptors remain to be
characterized regarding the nature of their ligand. Access to probes for
steroid receptors has enabled studies on mechanisms of regulation of receptor
gene expression. Deletion and II'Altational analysis of steroid receptor cDNAs
followed by expression in cells together with suitable reporter genes has
yielded a detailed knowledge about the functional significance of the various
domains the receptors are composed of. In certain cases, steroid resistance
in patients have been shown to be due to point mutations in the corresponding
steroid receptor genes resulting in non-functional receptors.
The availability of receptor cDNAs also makes it possible to express
receptors at high levels in procaryotic and eucaryotic cells. It is e.g.
possible to express the DNA-binding domain of the glucocorticoid receptor in
E.coli as a fusion protein with protein A which interacts specifically with
DNA. Such studies are necessary for production of sufficient quantities of
receptors to allow crystallization and X-ray crystallography for detailed
structural information.
Several steroid receptors can be shown to interact with a heat-shock protein
with a molecular weight of 90,000, hsp 90. The biological significance of
this interaction is currently under investigation but it is not inconceivable
that interaction between hsp 90 and the glucocorticoid receptor is necessary
for proper ligand binding. FurtheITIDre, it has been shown that heat
activation of the glucocorticoid receptor results in dissociation of hsp 90
from the receptor enabling it to interact with DNA.
An inpJrtant aspect of steroid control of gene expression is negative
10
regulation. The molecular mechanisms underlying this phenomenon are not yet
completely understood but one assumption that appears to be valid is that the
glucocorticoid receptor displaces same kind of positive regulatory factor
when interact.ing with DNA, thus resulting in decreased expression of the
regulated gene.
These and other themes were addressed at the symposium which thus provides an
exciting up-to-date review of the current front line of steroid receptor
research.