DOCUMENT RESUME ED 276 564 SE 047 537 AUTHOR Molinaro, Jane Anne TITLE The Relationship of Test Anxiety to Serum Beta-endorphin. INSTITUTION Ohio State Univ., Columbus. Graduate School. PUB DATE 86 NOTE 186p.; Ph.D. Dissertation, Ohio State University. Correlation tables contain small print. PUB TYPE Dissertations/Theses - Doctoral Dissertations (041) -- Reports - Research/Technical (143) EDRS PRICE MF01/PCO8 Plus Postage. DESCRIPTORS Anxiety; Biochemistry; College Science; Higher Education; *Human Body; *Physiology; *Premedical Students; Science Education; *Test Anxiety; *Test Wiseness IDENTIFIERS Endorphins; Hormones; Medical College Admission Test; *Science Education Research ABSTRACT This study attempted to determine if test anxiety is manifested in pre-medical students as a result of the Medical College Admission Test (MCAT) and if Beta-endorphin similarly responds to that type_of situational stress._Seventeen_participants completed_the Test Anziety_inventoryATAII_by Spielberger_et al. (1980) and donated 30_mi lf blood for Beta-endorphin analysis pwo days prior to the MCAT and also three days after the test. Student grade point averave (GPA) and number of science courses were other variables analyzed. Results indicated that TAI were more elevated pre-MCAT and Beta-endorphin wts higher after-the examination. Total TAI was significantly inversely related to GPA and there were positive correlations between GPA_and MCAT scores. Regression results_indicated_that_select_items_from TAI, GPA. and Beta-endorphin could be used to predict performance on particular_MCAT subsets and that MCAT scores were influenced by test anxiety. Appendices include copies of the TAI, procedures used in processing blood samples for Beta-endorphin, and complete correlation matrices. A 14-page bibliography is included. (ML) ***************************************************t******************* Reproductions supplied by EDRS are the best that can be made from the original document. *********************************************************************** THE RELATIONSHIP OF TEST ANXIETY TO SERUM BETA-ENDORPHIN "PERMISSION TO-REPRODUCE THIS MA RIAL HAS t N GRANTED BY By U11,0EPOTATMENT_0 F EDUCATION Improvement Office Of Educational fhispearch and INFORMATION EDUCATIONAL RESOURCES tat- - CENTER (ERIC) reproduced-Si XTfus-rdocUMent ha* bees Sister Jane Anne Molinaro; PhD; organization received -from the person Or originating it made to improve 0 Minor-chiitideLhave Peen The Ohio State University, 1986 orp_7_Puch quality TO_THE EDUCATIONALRESOURCEs in this docu, INFORMATION CENTER (ERIC):" Points of view ottomiontstated represent official Meat do_ not necessarily Professor Robert W. Howe, AdViter OERI posthon of ()WV The purpose of this study was to test pre-medical students before and after the Medical College Admission Test (MCAT) to determine the presence of test anxiety and its effect on MCAT scores. Seventeen participants reported two days prior to the MCAT to complete the Test Anxiety Inventory (TAi; by Spielberger et al., 1980, and to donate 30 ml of blocd. Subjects returned Results I the TM (with SubticaleS WorrY, three days pbst-MCAT to reptat the prOcedure. Plasma was eltracted from to post-MCAT seruin Emotionality, Total) were tabulated. Pre tamples and subted to Radioimmunoassay (R1A) tr; measure th c. amount of Beta-endorphin; Student GPA and number of ScieLce courses were other variables analyzed. It was hypothesized that the TM scores and Beta-endorphin levels would be significantly elevated as a result of stress associated with the MCAT, and test anxiety (as measured by It was further TM and Beta-endorphin) would be significantly to MCAT scores. related hypothesized that TM scbres would lie related to Ekta-endorphin and that GPA and Science courses would relate to MCAT and test anxiety measure& Results indicated that TM (subszales) were more elevated pre-MCAT and Beta-endorphin Test anxiety (TM Total and subicales) wag significantly was higher after the examination. Pre-MCAT Beta-endorphin was negatively corre- inversely related to performance on MCAT. lated with MCAT subsets of Chemistry and Science Problem& TM subscales and serum Beta- endorphin were not significantly correlated; several items of the TM were significantly related to Beta-endorphin. 2 page 2 There were positive correlations Total TAI Was Significantly inverse y related to GPA. between GPA and MCAT scores. Pre-MCAT Beta-endorphin results showed a significant positiVe relationthip with under- with undergraduate Chemistry coufses. correlation negativt Biology; a significant graduate Chemis- Post-MCAT Beta- endorphin data was Significantly inversely correlated with graduate try courses. Beta-endorphin could RegreStiOn results indicated that select items from the TM; GPA and MCAT scores were be used to predict perforniante on particular MCAT subtets and that influenced by test anxiety; The Appindicet Other conclusions and recommendations for further research are presented. Beta-endorphin; and include copies of the TAI, pitcedures used in processing blood samples for complete correlation matrices; The Relationship of Test Anxiety to Serum Seta-endorphin DISSERTATION Pro Tented in Ktrtial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Giswuate Scho Ol of The Ohio State University By Sister lane Anne Molinaro, A.B. The Ohio State University 1986 Dissertation Committee: Approved by Rob-ert W. Howe Stanley L Helgeson Gopi A. Teiwani Robert V. Howc Adviser Cm liege of Education: Theory and Practice DEDICATION To my deceased parents ACKNOWLEDGEMENTS Heartfelt gratitude is extended to the disiertation committee members: Dr. Roben W. Howe, whose competence and intelligence was augmented by patience; Dr. Gopi A. Tejwani, who generously shared laboratory facilities and extensive knowledge about serum Beta-endorphin research; Dr. Stanley L Helgeson who never complained about reading "still another copy." Special thanks to Jim Wagner from the Baker Computer Center for his technical assistance. I am deeply indebted to all my friends who saic4 "You will make id" gratefully acknowledge my natural and religious families whose prayen and I support have sustained me throughout this endeavor. I praise and thank God for His many blessings and pray that I will Above use this accomplishment for His greater Honor and Glory. TABLE OF CONTENTS PAGE DEDICATION ili ACKNOWULDGEMENTS iv VITA LIST OF TABLES viii UST OF FIGURES INTRODUCTION L 1 NWtcl for_ _the Stady 2 1 1 1 I Piirpose_cif _the_ Study_ 9 _ Statement of the _Problem 10 Definition of Terms 12 Delimitations 13 LiMitations 13 HYliothesfs_ 14 _ _ Plan of the Report 16 REVIEW OF THE LITERATURE 17 Characteristics of Test Anxiety 17 Characteristics of Serum Beta-endorphin 33 43 RESFARCH DESIGN kND PROCEDURES Population of Study 4.3 44 Instrumentation 50 Treatment Noiidures of Dina Collet_tion 51 Extraction of Beth-endorphin from Placma 52 53 Radioimmuncessay 60 IV. RESULTS 60 Sample Characteristics 67 Correlational Ani:yses 76 Regression Analyses Hypothesis and Problem Testing 81 86 SYNTHESIS, CONCLUSIONS AND RECOMMENDATIONS V. Synthfisis of Findings and Discussion 86 93 Recommendations VI. BIBLIOGRAPHY APPENDICES 109 A. TAI TEST FORM 110 TM TEST FOILM B. CONSENT TO SPECIAL TREATMENT OR PROCEDURE FORM 111 . . 112 CONSEN7 FORM 113 CONSENT FORM C TALC TREATMENT 114 SELICIC ACED 71MATIODU 115 D. MAIITRIALS USED IN RADIOIMMUNOASSAY 116 116 Antibody vl 117 Label_ _ 117 Standard Buffer 118 118 Charcoal F. 119 STALNDARD CURVE PREPARATION STUDY DATA 120 PILOT G. H. MEANS SD, AND DIFFERENCES BETWEEN MEANS FOR ALL 123 SUBJECTS L STkNDARD CURVE RESULTS 124 Plots of Experimental Data 125 MTANS OF THREE ASSAYS 128 130 K. SUBJECT PROFILES CORREIATION MATRICES 133 Correlation Table for All Subjects from J4AIN Study 134 Correlation_Table for Subjects from MAIN Study (Outliers 170 Removed) LIST OF TABLES PAGE TABLE 47 Test-Atetest Reliabilty for TAI Stores 1. 48 College Grads Correlations of TM Scores/Measures of Anxiety for ; 2. 49 Ccirrelations of TM S=res with Academic Achievement 3. 51 Frequency Chart for all Subjects 4. 62 Means and SD of TM Scores Pre and Pott=MCAT 5. 63 Differences Between Means of TAI Pre and Post-MCAT 6. 64 Means and SD of We Pre and Post MCAT 7; Milian) and Bateiine Differences Between Means of B-e (W/O 8. 65 Valuet e (W/O Outliers) Pre and Post- Differences Between Means of 9. 66 MCAT 67 Correlation Matrix for TM (W,U) ,- B-c GM (Pre-MCAT) 10. 68 (Post-MCAT) Correlation Matrix for TAI (W,E,T), We, GM 11; Correlation Matrix for TM (W, Ej T), GM, We, and Performance 12. 70 on MCAT (Pre-MCAT) Correlation Matrix for TAI (W, E, T), GM, B-e and Performance 13. 71 on MCAT (Post-MCAT) Correlation Matrix for TM (W,E,T) and Related Science Courses 14. 74 (Pro and Pat:MCAT) Corselation Matrix for B-e and Related Science Courses (Pre and 15. 75 Post-MCA.T) 77 Regression Analysis for MCAT-Biology 16. 78 Regression Analysis for MCAT .''hemistry 17. 1 0
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