Table Of Content

CONTENTS Charing Cross and Westminster Meeting Demonstrations Abnormal segment 'work' in the alternating heart of the anaesthetized pig M. Moutoussis, I.B. Gartside and M.J. Lab Interval dependence of mechanically induced early afterdepolarizations in ventricle of anaesthetized pigs M.J. Lab and D.J. Dick A device for atraumatic recording of regional electromechanical signals from the heart at surgery in man M.J. Lab, F.G. Harrison and G. Bennet Mechanical alternans induced in isolated guinea-pig papillary muscles by a combination of hypothermia and rapid pacing C.I. Spencer, M.J. Lab and W.A. Seed January 1891 - The Physiological Society at Charing Cross P.H. Ellaway, H.R. Hague, F. Harrison and E.M. Tansey Facilitation by mechanical cutaneous stimulation of muscle responses to transcranial magnetic stimulation in man N.J. Davey, P.H. Ellaway and D.W. Maskill Method for measuring the time course of twitch tension from a single motor unit in man P.H. Ellaway, I.B. Gartside, P. Romaiguére, 8.R. Rawlinson and E. Wells A computer-assisted practical class on isolated frog sciatic nerve: software and hardware Ivor B. Gartside Apparatus for the production of step changes in cuff pressure for venous occlusion plethysmography in man Ivor B. Gartside and John Gamble An electronic membrane osmometer to measure the oncotic pressure of microlitre quantities of oedema fluid D.O. Bates, J.R. Levick and P.S. Mortimer A re-assessment of the relationship between the protein concentration (CONC) and colloid osmotic pressure (COP) of fresh, heparinized human plasma (HP) and bovine serum albumin (BSA) J. Gamble A simple analogue circuit to provide a voltage proportional to ‘instantaneous’ minute ventilation from respiratory airflow for use in studies with targeted breathing S.H. Moosavi, L. Adams and K. Murphy ii A simple technique to study human laryngeal respiratory muscle action (LRMA) during different tasks in health and disease BO Tin TE TN RN niki idsscssinissinrsinsccnvenncsomiicnnieetebiicetaiokeanehciailien 14 Comparison of within-breath variations in left ventricular stroke volume during positive-pressure and spontaneous ventilation in man A a i TI CO ih ict btcssiciinssisceicaSs eeneinsbvancidnsvnsatmeeaaneiiennsepee 15 Generation and measurement of rapid changes in airway pressure in man J.A. Innes, R.L. Horner and A. Guz Facilitation of the diaphragmatic response to transcranial magnetic stimulation with peripheral nerve stimulation K. Murphy, L. Adams, A. Mier, D. Maskill, D. Lobo and A. Guz A protocol for studying the effects of changes in orthostatic pressure on the responses of the human limb to cuff inflation using mercury-in-Silastic strain gauge plethysmography J. Gamble, F. Christ and I.B. Gartside Communications Restitution of mechanical force during alternans in isolated guinea-pig papillary muscles C.I. Spencer, M.J. Lab and W.A. Seed Effect of diastolic membrane potentials on twitch force in isolated ferret papillary muscle P. Ariock, W.1.M. Noble:and B.: Woblfart oc... ccc cicccssccccssesccscecessccocdescccncssvddeacerccovecese 19 Effect of B-adrenergic agonists on maximum output pressure in the isolated rabbit heart during hyperkalaemia David J. Paterson, Stewart Leitch, Simon M. Phillips and Hilary F. Brown Endothelin-stimulated inositol phosphate formation in aorta and cultured aortic cells from spontaneously hypertensive rats G.F. Nixon, F. Pasanisi, C. Pryce, C.A. Hamilton, R.M. Wadsworth and J.L. Reid A comparison of the action of angiotensin II, 5-hydroxytryptamine and adenosine triphosphate on ionic currents in rabbit single ear artery cells A.D. Hughes and T.B. Bolton Effects of progesterone on contractility of rat isolated vascular smooth muscle Catherine Huyton and Helen L. Leathard Measurement of regional cerebral blood flow during volitional breathing using positron emission tomography in man J.G. Colebatch, L. Adams, K. Murphy, A.J. Martin, A.A. Lammertsma, H.J. Tochon-Danguy, J.C. Clark, K.J. Friston and A. Guz Carotid sinus baroreceptor reflex during the Valsalva manoeuvre in man S.A. Choksy, D.J. Collier and J.E. Angell-James .0........0..cccccccsscccesseceeesseecessseeeeeeees 2 _ The influence of metabolic acidosis on the sensation of breathlessness in man during exercise and hypercapnia R. Lane, L. Adams and A. Guz The effects of imposed breathing frequencies on gas exchange during moderate exercise in man 8. Bojanic, G.J.C. Burkill and 8.J. Walter Modelling the human ventilatory response to isocapnic hypoxia Rosemary Painter, Saeed Khamnei and Peter A. Robbins The effect of short-term hypoxic exposure on ventilation and carotid body dopamine blockade in anaesthetized rats I TNE oiccccisistintisniiRvieinniniougbicaadnsaanaicgebiias schealseasc aansab mnaeaahne wkbaac i Effect of cortical stimulation on the execution of fast complex arm movements in man A. Berardelli, M. Inghilleri, G. Gruccu and M. Manfredi Motor performance following the bilateral lesion of the supplementary motor area (SMA) in man J.A. Obeso, J. Artieda, A. Oliver, J. Muruzabal and L. Barraquer-Bordas Longitudinal study of babies developing cerebral palsy: neuro-physiological signs of spasticity with normal conduction in the fastest fibres of the corticospinal pathway M.C. O'Sullivan, V. Ramesh, 8. Miller and J.A. Eyre Reorganization in motor pathways following a large congenital hemispheric lesion in man: different ipsilateral motor representation areas for ipsi- and contralateral muscles L.G. Cohen, T. Zeffiro, S. Bookheimer, E.M. Wassermann, P. Fuhr, J. Matsumoto, C. Toro and M. Hallett Physiological studies in a patient with mirror movements and agenesis of the corpus callosum J.C. Rothwell, J. Colebatch, T.C. Britton, A. Priori, P.D. Thompson, B.L. Day and C.D. Marsden Raised motor cortical threshold associated with bradykinesia as revealed by transcranial magnetic stimulation in normal man and Parkinson’s disease N.J. Davey, J.P.R. Dick, P.H. Ellaway and D.W. Maskill The respiratory responses to magnetic stimulation of the cortex in sedated and anaesthetized baboons W.A. Karczewski, K. Budzinska, K. Glowicki, M. Malinowska and M. Ryba Unilaterality of the motor cortical representation of the human diaphragm T. Similowski, M. Catala, B. Orcel, J-C. Willer and J-P. Derenne Enhancement of the H reflex after anaesthetic blockade of afferents from antagonist muscles in man J. Artieda, P. Quesada and J.A. Obeso Modulation of excitatory and inhibitory reflexes in muscles of the shoulder in normal subjects and stroke patients performing a cyclical task 8. Miller and R.D. Plant iv Delayed motor unit discharges associated with a high degree of short-term synchrony in isometric contractions in man J.R. Baker, N.J. Davey, P.H. Ellaway and C.L. Friedland Phases of depressed axonal function after spinal trauma in anaesthetized cats V.E. Amassian and M. Jayachandra Effects of analytical and visuo-spatial cognitive tasks on spatio-temporal characteristics of the human auditory evoked potential E.A. Davies, M.F. Regan, P.J. McCullagh, R.J. McClelland and W.F.M. Wallace Studies correlating NMDA-stimulated efflux of (*H]dopamine from rat brain striatal cores with conditioned avoidance behaviour M. Smith, D. Rakhit, D.R. Patel and J.M. Turnbull Induction of sarcoplasmic reticulum Ca?*-ATPase synthesis by thyroid hormone in the L6 muscle cell line is potentiated by insulin-like growth factor-I (IGF-I) A. Muller, C. van Hardeveld, W.S. Simonides and J. van Rijn Metabolite changes, without intracellular acidification, are sufficient to cause the fall of force in hypoxia in isolated rat uterus Susan Wray Lactate efflux from fatigued single muscle fibres of Xenopus laevis A.S. Nagesser, W.J. van der Laarse and G. Elzinga Rate of oxygen consumption of single Xenopus muscle fibres is proportional to oxygen taken up in recovery W.J. van der Laarse and G. Elzinga Force—velocity relation for shortening and lengthening of fatigued isolated muscle fibres from frog N.A. Curtin and K.A.P. Edman Singular value decomposition of mechanical signals from anaesthetized pig heart M. Moutoussis, A.V. Holden and M.J. Lab Plasma thymulin concentrations in cardiac patients: involvement with the ypothalamic-pituitary-adrenal axis B. Safieh, G.E. Venn, M.A. Ritter, S. Singh, J.C. Buckingham and M.D. Kendall The influence of lipocortin 1 on hypothalamo-pituitary-adrenocortical (HPA) function in the rat in vivo and in vitro H.D. Loxley, A-M. Cowell, P.O. Cover, R.J. Flower and J.C. Buckingham Interactions between GABAergic and somatostatin-producing cells in cultures of fetal rat hypothalami G.E. Gillies, 8. Guldenaar and K. Davidson Contractile myogenic response of the coronary vessels in the anaesthetized dog D. Gattullo, R.J. Linden, G. Losano, P. Pagliaro and P. Vono Inhibition of coronary arterial platelet thrombosis in the anaesthetized dog by antagonism of the 5HT, receptors B. Cox, 8.J.G. McAuliffe, M.I.M. Noble and H.M. Snow Changes in superior mesenteric artery blood flow during sympathetic activation in normal subjects K. Ray Chaudhuri, T. Thomaides, P. Hernandez and C.J. Mathias Effect of phentolamine on initial cardiovascular response to isocapnic hypoxia in intact and carotid sinus denervated fetal sheep D.A. Giussani, J.A.D Spencer, P.J. Moore and M.A. Hanson An assessment of the correlation between cyclic changes in limb circumference and cardiovascular instability using mercury in rubber strain gauge plethysmography (MSG) in man F. Christ, I. Gartside, W. Kox and J. Gamble Changes in colloid osmotic pressure (COP) during continuous veno-venous or arterio-venous haemodialysis (CVVHD/CAVHD) in man F. Christ, S. Weidel, K. McIntyre, T. Jameson, W.J. Kox and J. Gamble The effect of high frequency oscillation on the non-Newtonian properties of sputum C. Moores and A. Davies Uncoupling of proximal tubular handling of sodium and phosphate in man F.H. Noormohamed and A.F. Lant A micropuncture study of renal lithium handling in anaesthetized rats D.G. Shirley, S.J. Walter and B. Sampson Effect of formate and oxalate on fluid reabsorptive rate (J,) in the proximal convoluted tubule of the anaesthetized rat M. Wareing and R. Green Effects of diabetes mellitus on calcium handling by the proximal tubule and loop of Henle in anaesthetized rats H.O. Garland, P.J. Harris and T.0. Morgan. .............::cccccccccssseceesseeceeneeeeeseeeeeseeeeenes 63 Renal function in papillary, necrotic chronically instrumented, conscious rats: effects of bumetanide J. Plange-Rhule, A. Marshall, D. Robinson, A. Turnbull and J.C. Atherton The effect of several classes of diuretic on water (J,), sodium (J,,) and potassium (J,) transport in the anaesthetized rat loop of Henle (LOH) in vivo G. Capasso, G. Giebisch and R. Unwin Natriuretic effect of arginine vasopressin in papillary necrotic, chronically instrumented, conscious rats J. Plange-Rhule, A. Marshall, D. Robinson, A. Turnbull and J.C. Atherton The natriuretic actions of the neurohypophysial hormones in the rat R.J. Windle, M.L. Forsling and J. Judah A novel atrial peptide receptor in the kidneys of 3-week-old spontaneously hypertensive rats vi Effect of atrial natriuretic factor (ANF) on steroidogenesis in teleost fish, the flounder, Platichthys flesus, and the trout, Salmo gairdneri, in vivo and in vitro D.E. Arnold-Reed and R.J. Balment Stimulation of atrial natriuretic factor (ANF) secretion from isolated guinea-pig atrial strips P.F. Speake, 8S. Pirie and R. Green Royal Free Meeting Demonstrations Extracellular recordings from lower thoracic and lumbar sympathetic preganglionic neurones in the anaesthetized rat S-Y. Zhou and M.P. Gilbey Anterograde labelling of soine short CNS pathways under acute experimental condi- tions in the rat and cat J. Deuchars, R.M. Sykes, H.I. Choudhury and P.N. Izzo Integrating cellular and haemodynamics events in didactic simulations of cardiovascular dynamics J. Lefevre and P. Van Roey Measurement of the ionic composition of urine with ion-selective electrodes S.E.M. Langley, J. Graves and C.H. Fry The use of Mag-Fura-2 to measure the intracellular Mg** concentration, [Mg?*],, in isolated guinea-pig ventricular myocytes A. Buri, C.H. Fry, J.A.S. McGuigan, D. Noble, T. Powell and V.W. Twist Anatomical identification of the origin and chemistry of synaptic inputs onto vagal motoneurones in the rat and cat J. Deuchars and P.N. Izzo Communications The effect of glutamate receptor antagonists on rat thermoreactive hypothalamic neurones M.J. Parton, R.J. Gayton and D.C.M. Taylor Activation of GABA, receptors causes pre- and postsynaptic effects in the rat nucleus tractus solitarius (NTS) in vitro P.A. Brooks, A.J. Bradley, 8. Glaum and R.J. Miller Demonstration of a monosynaptic pathway from the nucleus tractus solitarius to regions of the ventrolateral medulla with specific reference to vagal motoneurones in the nucleus ambiguus of the anaesthetized cat J. Deuchars and P.N. Izzo Rhythmic activity of neurones in the dorsomedial region of the nucleus tractus solitarius (NTS) of the rat in vitro J.F.R. Paton, W.T. Rogers and J.S. Schwaber ..................cccccccccccecceeseeeeeeeceeeeeeeeeeeees 81 Inhibitory influence from the ventrolateral periaqueductal grey matter on rostral ventrolateral medullo-spinal neurones in anaesthetized rats T.A. Lovick Microinjection of phenylephrine into the intermediolateral cell column elicits an increase in cardiac sympathetic nerve activity in the anaesthetized cat S.A. Marks and M.P. Gilbey Examination of the sympatho-excitatory action of the 5-HT, agonist, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI), on cardiac sympathetic nerve activity in the anaesthetized cat A.G.Ramage, 8.L.Shepheard, D.Jordan and M.C.Koss Lack of vascular pressure equilibrium during the measurement of mean circulatory filling pressure in urethane-anaesthetized, ganglion-blocked rats Andrew J. Rankin and Linong Cheng Reflex vasodilatation to independent changes in aortic root and left ventricular pressures in the anaesthetized dog M.J. Drinkhill, J.K.A. Al-Timman and R. Hainsworth The stroke volume reduction by baroreceptor activation in human volunteer subjects is mediated by the vagus B. Casadei, T. Meyer, A.J.S Coats, J. Conway and P. Sleight The role of vasopressin in the regional vascular responses induced by hypocapnic hypoxia in the anaesthetized rat Anne-Marie Louwerse and Janice M Marshall Effects of endothelin-1 on carotid baroreceptor and chemoreceptor activity in the anaesthetized cat D.S. McQueen, M.R. Dashwood and R.M. Sykes An investigation of some of the mechanisms linking cardiac action potential duration to mechanical events in the isolated guinea-pig heart and the heart of the anaesthetized dog D.S. Coulshed, M.J. Drinkhill, J.C. Cowan and R. Hainsworth Chemo-mechanical coupling in the left ventricular dynamic compliance J. Lefevre Blood pressure and heart rate variability in response to controlled respiration in human volunteers undergoing sustained +Gz acceleration I. McKenzie Vascular responses evoked by isometric hand grip in the contralateral forearm of human subjects Christina Cotzias and Janice M. Marshall Abnormal vascular responses in the diabetic foot are a result of neuropathy and not microangiopathy M.J. Stevens, M.E. Edmonds and P.J. Watkins Viii Inability to constrict actively the superior mesenteric artery in human autonomic failure K. Ray Chaudhuri, T. Thomaides and C.J. Mathias ...............cccccseseseesssseeeeeeesneeeeees 95 Protein kinases modulate Ca sensitivity of cardiac myofilaments in rat skinned cells Odile Clement, Michel Puceat and Guy Vassort a-Adrenoceptors modulate intracellular pH in single rat isolated ventricular cardiomyocytes A. Terzic, Odile Clement and M. Puceat Possible involvement of a chloride conductance in the transient outward current of ferret ventricular myocytes A. Bouron, D. Potreau and G. Raymond A cholera toxin sensitive G-protein mediates the P,-purinergic stimulation of calcium current in rat ventricular cells Frederique Scamps and Guy Vassort Desensitization to acetylcholine in single sino-atrial node cells isolated from the rabbit heart H. Honjo, I. Kodama and M.R. Boyett The kinetics of desensitization to acetylcholine in single atrial cells isolated from the guinea-pig heart W.-J. Zang and M.R. Boyett Creatine kinase in the gestating guinea-pig uterus J.F. Clark, Z. Khuchua, A.V. Kuznetsov and R. Ventura-Clapier Evidence that nitric oxide mediates human penile erection R.S. Pickard, P.H. Powell and M.A. Zar Single Ca?*-activated K* channels recorded from human cultured myometrial cells M.L.J. Ashford, R.N. Khan and S.K. Smith Calcium currents in bovine adrenal chromaffin cells P. Charlesworth, G. Pocock and C.D. Richards Single potassium channel currents in rat hippocampal neurones maintained in culture K.T. Wann, G. Pocock and C.D. Richards Effects of acetylcholinesterase on the membrane properties of guinea-pig cerebellar Purkinje cells recorded intracellularly in vitro M.E. Appleyard and H. Jahnsen Uptake of sulphur-containing amino acids by the electrogenic glutamate uptake carrier in glial cells isolated from the salamander retina Muriel Bouvier, Barbara Miller, Marek Szatkowski and David Attwell Pertussis toxin pretreatment increases glutamate release and dihydropyridine binding sites in cultured rat cerebellar granule neurones E. Huston, M.S. Fazeli, H.A. Pearson, V.H. Norris, M. I. Sweeney and A.C. Dolphin Voltage-dependent block by /-cis-diltiazem of cyclic GMP activated conductance in rod bipolar cells of dogfish retinal slices R.A. Shiells and G. Falk Rectification in identified rat sympathetic preganglionic neurones in vitro A.E. Pickering, D. Spanswick and 8.D. Logan Inhibitory postsynaptic potentials in sympathetic preganglionic neurones (SPN) in rat spinal cord in vitro D. Spanswick and 8.D. Logan Local circuit excitatory and inhibitory connections in slices of rat thalamus Alex M. Thomson and David C. West Assessment of hepatic microcirculation by laser Doppler flowmetry in the in situ perfused liver of anaesthetized rats: effect of perfusate haematocrit A.M. Wheatley and N.E. Almond The effects of sustained isocapnic hypoxia during steady-state exercise in man J.J. Pandit and P.A. Robbins Effect of propranolol on arterial plasma potassium ([K*],) and ventilation during exercise in man D.J. Paterson, J. Conway and P.A. Robbins Pharmacological characteristics of adrenal responses to acetylcholine in conscious calves A.V. Edwards and C.T. Jones The effect of a corticotrophin releasing factor antagonist on adrenal function in conscious calves C. T. Jones and A. V. Edwards The effect of intracerebroventricular injection of hypertonic solutions on local cerebral glucose utilization (LCGU) in the conscious rat M.R. Achaaban, T. Wells, H.K. Richards and M.L. Forsling Selective activation by capsaicin of polymodal nociceptors in the rat hind paw skin saphenous nerve in vitro and antagonism by capsazepine N. Seno and A. Dray Histidine-stimulated “Zn transport at the blood-brain barrier of the anaesthetized rat S. Buxani and J. Adu An in situ vascular perfusion method for measuring blood-retinal barrier permeability in the anaesthetized rat J. Gratton, 8. Lightman and M.W.B. Bradbury Effect of poly-L-lysine on the transepithelial permeability across cultured epithelial monolayers of MDCK cells G.T.A. McEwan, B.H. Hirst and N.L. Simmons Antibodies to amiloride-sensitive sodium channels reveal the possible location of the mechanoelectrical transducer channels in guinea-pig cochlear hair cells C.M. Hackney and D.N. Furness x Intracellular free calcium concentration in human sweat duct cells in vitro: increases and oscillations induced by agonists P. Steen Pedersen* and J. Hedemark Poulsen Reduced brush-border chloride permeability is responsible for the enhanced sugar transport in isolated jejunal enterocytes from insulin hypoglycaemic rats: an in vitro study E.S. Debnam and G. Chowrimootoo Ethanol and hexose transport across the brush border and basolateral membranes of isolated rat jejunal enterocytes E.S. Debnam Effects of an epithelial Cl channel blocker on whole cell voltage clamp and patch clamp recordings from a human astrocytoma in culture M. Baker, N.S. Crouch and J.E. Arrowsmith Observations on zinc deficiency in the young rat J. Adu, 8. Betts and M.W.B. Bradbury The effects of posterior cerebellar vermal lesions on the cardiovascular components of the alerting response in the rabbit D.J. Bradley, M.A. Goring, A. La Noce and K.M. Spyer Actions of 5-hydroxytryptamine on dorsal vagal motoneurones of the rat in vitro P.A. Brooks Carotid sinus baroreceptor modification of mean circulatory filling pressure in the anaesthetized rabbit P.W. Humphreys and N. Joels Respiratory modulation of the carotid sinus baroreceptor reflex in man D.J. Collier, M.D. Ralph, V.L. Zadorozny and J.E. Angell-James The effect of adenosine deaminase on pacing-induced coronary functional hyperaemia in denervated hearts of anaesthetized dogs F. Karim and I.P. Goonewardene ['*Ijendothelin binding sites and endothelin-like immunoreactivity in isolated human blood vessels: a role in atherosclerosis ? M.R. Dashwood, M.J. Collins, J.R. Muddle, R.M. Sykes, 8S. Theodoropoulos and M.H. Yacoub Pathophysiology of photochemically induced retinal ischaemia in the anaesthetized cat T. Asai, E.A. Dawes, M.W. Hankins and H. Ikeda Distribution of glutamate immunoreactive neurones in the V main sensory and V nucleus oralis of the rat Sikha Saha, Kwabena Appenteng and Trevor F.C. Batten Topographical discontinuities modify the outgrowth of leech Retzius neurones grown in culture R.J.A. W'lson, S.E. Blackshaw and J.A.T. Dow