Table Of Content

General Physiology is the study of biolo mechanisms Grouch ea poei nvestig which decipher the mole mechanisms underlyingb iological functioo n at levels of organiz The mission of the J Val of Gener is to publish ar rtic lesa e be eluc The Journal of General Physiology \b iologica } chemibcraola,d oprh yspinoylso gCiac al esic han Vol 138 © No 1 e July 2011 Meeting Summary 1 The 64th Symposium of the Society for General Physiologists: optogenetics and superresolution microscopy take center stage. Graham C.R. Ellis-Davies and Edward N. Pugh Jr. Commentaries 13 Dynamical systems theory in physiology. Arthur Sherman Articles 21 lonic mechanisms and Ca** dynamics underlying the glucose response of pancreatic § cells: a simulation study. Chae Young Cha, Yasuhiko Nakamura, Yukiko Himeno, JianWu Wang, Shinpei Fujimoto, Nobuya Inagaki, Yung E Earm, and Akinori Noma Time-dependent changes in membrane excitability during glucose-induced bursting activity in pancreatic B cells. Chae Young Cha, Enrique Santos, Akira Amano, Takao Shimayoshi, and Akinori Noma The pathway and spatial scale for MscS inactivation. Kishore Kamaraju, Vladislav Belyy, lan Rowe, Andriy Anishkin, and Sergei Sukharev Functional properties and toxin pharmacology of a dorsal root ganglion sodium channel viewed through its voltage sensors. Frank Bosmans, Michelino Puopolo, Marie-France Martin-Eauclaire, Bruce P. Bean, and Kenton J. Swartz An analysis of the relationships between subthreshold electrical properties and excitability in skeletal muscle. Thomas H. Pedersen, Christopher L.-H. Huang, and James A. Fraser Relationships between resting conductances, excitability, and t-system ionic homeostasis in skeletal muscle. James A. Fraser, Christopher L.-H. Huang, and Thomas H. Pedersen Na*,K*-pump stimulation improves contractility in isolated muscles of mice with oe+ seretage essed‘ parted: paralysis. Torben Clausen, Ole Baekgaard Nielsen, Johannes D. Clausen, Thomas Holn " Lawrence J. Hayward Abstracts of Papers at the 65th Annual Meeting of The Society of General Physiologists: Mitochondrial physiology and medicine Index to authors of abstracts Cover picture: Four bifurcation diagrams and corresponding time simulations of bursting made with a Chay—Keizer model of a cell. In the top of each pair of panels, the solid black line represents stable states of V,, as a function of (clamped) Ca, the dashed and dotted lines represent unstable equilibria and unstable oscillations, and the blue lines represent burst trajectories. The bottom of each pair of panels shows the corresponding time-dependent changes in V,,, with Ca free to vary. Upper left: square wave without spikes; upper right: B cell-like bursting; lower left: pituitary-like bursting; lower right: bursting without bistability but with two slow variables, as in the R15 neuron of Aplysia (see commentary by Sherman, 13-19). General Physiology is the study of biological mechanisms ercugh analytical investigations, which decipher the molecular and cellular mechanisms underlying biological function at all levels of organization. The mission of the Journal of General Physiology is to publish articles that elucidate important biological, chemical, or physical mechanisms of The Journal of General Physiology broad physiological significance. Vol 138 ¢ No 2 © August 2011 Editorials 131 Redefining our menu: Communications and Reviews. Edward N. Pugh Jr. Commentaries 133 Where's the gate? Gating in the deep pore of the BKc, channel. Daniel H. Cox and Toshinori Hoshi Articles 137 Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating. Xixi Chen and Richard W. Aldrich 155 R1 in the Shaker $4 occupies the gating charge transfer center in the resting state. Meng-chin A. Lin, Jui-Yi Hsieh, Allan F. Mock, and Diane M. Papazian 165 Alignment of transmembrane regions in the cystic fibrosis transmembrane conductance regulator chloride channel pore. Wuyang Wang, Yassine El Hiani, and Paul Linsdell 179 Local constraints in either the GluN1 or GluN2 subunit equally impair NMDA receptor pore opening. lehab Talukder and Lonnie P. Wollmuth 195 Spatial organization of RYRs and BK channels underlying the activation of STOCs by Ca’* sparks in airway myocytes. Lawrence M. Lifshitz, Jeffrey D. Carmichael, F. Anthony Lai, Vincenzo Sorrentino, Karl Bellvé, Kevin E. Fogarty, and Ronghua ZhuGe D4cpv-calsequestrin: a sensitive ratiometric biosensor accurately targeted to the calcium store of skeletal muscle. Monika Sztretye, Jianxun Yi, Lourdes Figueroa, Jingsong Zhou, Leandro Royer, and Eduardo Rios Measurement of RyR permeability reveals a role of calsequestrin in termination of SR Ca** release in skeletal muscle. Monika Sztretye, Jianxun Yi, Lourdes Figueroa, Jingsong Zhou, Leandro Royer, Paul Allen, Gustavo Brum, and Eduardo Rios Thiazolidinedione insulin sensitizers alter lipid bilayer properties and voltage-dependent sodium channel function: implications for drug discovery. Radda Rusinova, Karl F. Herold, R. Lea Sanford, Denise V. Greathouse, Hugh C. Hemmings Jr., and Olaf S. Andersen Communications 271 Xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings. Denice O'Connell, Karen Mruk, Jessica M. Rocheleau, and William R. Kobertz 279 Correction Cover picture: Antibiotic resistance is a game of tic-tac-toe: Harvested Xenopus laevis oocytes (O's) visibly damaged (black foci on animal pole) by multi-drug-resistant bacteria; scanning electron micrographs (X’s) of the multi-drug-resistant bacteria on the oocyte’s surface that are susceptible to a ciprofloxacin/amikacin antibiotic cocktail. Judicious antibiotic usage should prevent further drug resistance while providing healthy oocytes for the investigation of ion transport proteins (see communica- tion by O'Connell et al., 271-277). General Physiology is the study of biological mechanisms through analytical investigations which decipher the molecular and cellular mechanisms underlying biological function at all ~ — levels of organization The mission of the Journal of General Physiology is to publish articles that elucidate important biological, chemical, or physical mechnar s of The Journal of General Physiology COD PyenO pC Vol 138 * No 3 e September 2011 Perspectives on: Information coding in mammalian sensory physiology 281 Perspectives on: Information coding in mammalian sensory physiology. Robert A. Farley and Alapakkam P. Sa 283 Nonlinear spatial encoding by retinal ganglion cells: when 1 + 1 # 2. Greg Schwartz and Fred Rie e© ke 291 Probing mammalian touch transduction. Diana M. Bautista and Ellen A. Lumpkin 303 Response kinetics of olfactory receptor neurons and the implications in olfactory coding. Johannes Reisert and Haiging Zhao 311 Inhibitory synaptic mechanisms underlying functional diversity in auditory cortex. Li |. Zhang, Yi Zhou, and Huizhong W. Tao Articles oe Mechanism for modulation of gating of connexin26-containing channels by taurine. Dar Liang Tao, Paul L. Sorgen, and Andrew L. Harris 341 Coexpression and activation of TRPV1 suppress the activity of the KCNQ2/3 channel. Xu Torben R. Neelands, Steve McGaraughty, Prisca Honore, Carol S. Surowy, and Zhang 53 53 An acetylcholine receptor lacking both y and « subunits mediates transmission in zebrafish slow muscle synapses. Rebecca Mongeon, Michael Walogorsky, Jason Urban, Gail Mandel, Fumihito Ono, and Paul Brehm Letters to the Editor 367 Can selectivity be functionally modulated in ion channels? Riccardo Albina Locarno, Rita Canella, Oscar Sacchi, and Maria Lisa Rossi 371 A minimalist model for ion partitioning and competition in a K* channel selectivity filter. Ste Thomas Kloss, Sascha Tayefeh, and Gerhard Thie lon selectivity of proteins: lessons from molecular dynamics simulations on valinomycin. George | Cover picture: Modification of the cytoplasmic, carboxyl-terminal domain (CT) of connexin26 affects modulation of channel activity by taurine (left, from top: orbital structure, electrostatic potential, local ionization potential). Energy-minimized conformations of the native CT (center) with a four amino acid addition (right) are shown. NMR shows that taurine interacts only with the cytoplasmic loop domain (CL), not the CT. In the presence of taurine, the pore closes; the four amino acid extension causes pore narrowing. Results suggest that CL-CT interaction affects gating, and that taurine modifies this interaction (see article by Locke et al., 321-339). General Physiology is the study of biological mechanisms through analytical investigations, which decipher the molecular and cellular mechanisms underlying biological function at all levels of organization. The mission of the Journal of General Physiology is to publish articles that elucidate important biological, chemical, or physical mechanisms of The Journal of General Physiology broad physiological significance. Vol 138 ¢ No 4 © October 2011 Commentaries 377 Not what you thought: How H* ions combine with taurine or other aminosulfonates to close Cx26 channels. Michael V.L. Bennett Articles 381 Elevated intracellular Ca** reveals a functional membrane nucleotide pool in intact human red blood cells. Teresa Tiffert and Virgilio L. Lew 393 The Na conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers. Marino DiFranco and Julio L. Vergara 421 Potassium-selective block of barium permeation through single KcsA channels. Kene N. Piasta, Douglas L. Theobald, and Christopher Miller 437 Calcium-dependent block of P2X7 receptor channel function is allosteric. Zonghe Yan, Anmar Khadra, Arthur Sherman, and Stanko S. Stojilkovic 453 5-HT3 receptor ion size selectivity is a property of the transmembrane channel, not the cytoplasmic vestibule portals. Nicole K. McKinnon, David C. Reeves, and Myles H. Akabas Journal Club 467 To be, or not to be two sites: that is the question about LeuT substrate binding. Nicolas Reyes and Sotiria Tavoulari Cover picture: Scheme depicting a submembrane ATP-ADP pool, restricted diffusionally from bulk cytoplasmic enzymes and nucleotides in intact human red blood cells. Discrepancies between measured and predicted nucleotide changes induced by Ca** pump-saturating calcium loads can be explained by the hypothesis that the submembrane ATP pool is the immediate source of energy for the plasma membrane sodium and calcium pumps (see article by Tiffert and Lew, 381-391). General Physiology is the study of biological mechanisms through analytical investigations which decipher the molecular and cellular mechanisms underlying biological function at all levels of organization The mission of the Journal of General Physic is to publish articles tha }. KDA;I OIOGICaml=,! C~ NeMiICc ¢ The Journal of General Physiology Vol 138 ¢ No 5 ¢ November 2011 Editorials 473 Associate editor: Sharona Gordon. Edward N. Pugh Jr Articles 475 Molecular dynamics simulations of the Cx26 hemichannel: Evaluation of structural models with Brownian dynamics. Taekyung Kwon, Andrew L. Harris, Angelo Rossi, and Thaddeus A. Bargiello 495 Structural basis for the channel function of a degraded ABC transporter, CFTR (ABCC7). Yonghong Bai, Min Li, and Tzyh-Chang Hwang 509 Hysteresis of gating underlines sensitization of TRPV3 channels. Beiying Liu, Jing Yao, Michael X. Zhu, and Feng Qin 521 KCNQ1 subdomains involved in KCNE modulation revealed by an invertebrate KCNQ1 orthologue. Koichi Nakajo, Atsuo Nishino, Yasushi Okamura, and Yoshihiro Kubo Influence of pH on Ca** current and its control of electrical and Ca”* signaling in ventricular myocytes. Noriko Saegusa, Emma Moorhouse, Richard D. Vaughan-Jones, and Kenneth W. Se WU Cover picture: (Left) The grand canonical Monte Carlo Brownian dynamics (GCMC/BD) system used to simulate ion permeation through molecular dynamics—equilibrated and non-equilibrated structural models of connexin-26 (Cx26) hemichannels. (Right) Depiction of sites at which charges were changed in the GCMC/BD studies to mimic the effects of previously reported MS-identified Cx26 protein modifications. Blue, N-acetylation of Met1; red, acetylation of lysine residue (K15) in the NT; green, acetylation of six internal lysine residues in CL; orange, y-carboxylation of three giutamates (see article by Kwon et al., 475-493). General Physiology is the study of biological mechanisms Pacuan analytical investigations, which decipher the molecular and cellular mechanisms underlying biological function at all levels of organization. The mission of the Journal of General Physiology is to publish articles that elucidate important biological, chemical, or physical mechanisms of The Journal of General Physiology broad physiological significance. Vol 138 ¢ No 6 e December 2011 Meeting Summaries 563 The 65th Symposium of the Society for General Physiologists: Energizing research in mitochondrial physiology and medicine. Shey-Shing Sheu, Robert T. Dirksen, and Edward N. Pugh Jr. 569 Paul F. Cranefield Award to Rikard Blunck Articles 571 Thermodynamic coupling between activation and inactivation gating in potassium channels revealed by free energy molecular dynamics simulations. Albert C. Pan, Luis G. Cuello, Eduardo Perozo, and Benoit Roux 581 hERG1a N-terminal eag domain-containing polypeptides regulate homomeric hERG1b and heteromeric hERG1a/hERG1b channels: A possible mechanism for long QT syndrome. Matthew C. Trudeau, Lisa M. Leung, Elon Roti Roti, and Gail A. Robertson 593 Mg** binding to open and closed states can activate BK channels provided that the voltage sensors are elevated. Ren-Shiang Chen, Yanyan Geng, and Karl L. Magleby 609 Cl” concentration changes and desensitization of GABA, and glycine receptors. Urban Karlsson, Michael Druzin, and Staffan Johansson 627 The core domain as the force sensor of the yeast mechanosensitive TRP channel. Zhenwei Su, Andriy Anishkin, Ching Kung, and Yoshiro Saimi Communications 641 Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes. Grigory Maksaev and Elizabeth S. Haswell Corrections 651 Importance of hydration and dynamics on the selectivity of the KcsA and NaK channels. Sergei Yu. Noskov and Benoit Roux 653 Perspectives on: Information and coding in mammalian sensory physiology. Diana M. Bautista and Ellen A. Lumpkin Cover picture: The energy landscapes in the left and center panels reveal the importance of residue Phe103 as an allosteric link between the activation and inactivation gates of the KcsA potassium channel. As the intracellular gate transitions from closed (left) to open (center), the minimum of the energy landscape shifts to favor the rotameric state of Phe103 observed in the open crystal conformation. The right panel illustrates the steric clash that causes the Phe103 to change conformation after the intracellular gate opens (see article by Pan et al., 571-580).