The Influence of Antibiotics on the Host-Parasite Relationship II

302 Pages·1985·8.23 MB·English

by  D. S. Stephens

The Influence
of Antibiotics
on the Host -Parasite
Relationship II
Edited by
D. Adam H. Hahn and W. Opferkuch
With 98 Figures and 60 Tables
Springer-Verlag
Berlin Heidelberg New York Tokyo
Professor Dr. Dr. med. Dieter Adam
Kinderklinik der Universitat Miinchen
LindwurmstraBe 4
8000 Miinchen 2
Professor Dr. med. Helmut Hahn
Institut fiir Medizinische Mikrobiologie
Freie Universitat Berlin
Hindenburgdamm 27
1000 Berlin 45
Professor Dr. med. Wolfgang Opferkuch
Abteilung fiir Theoretische und Klinische Medizin
Lehrstuhl fiir Medizinische Mikrobiologie und Immunologie
Ruhr-Universitat Bochum
UniversitatsstraBe 150
4630 Bochum 1
ISBN-13: 978-3-642-70750-6 e-ISBN-13: 978-3-642-70748-3
DOl: 10.1007/978-3-642-70748-3
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© Springer-Verlag Berlin Heidelberg 1985
Softcover reprint of the hardcover 1st edition 1985
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2127/3140-543210
Preface
The Second International Symposium on "The Influence of Antibiotics on the Host
Parasite Relationship" was held in Munich, F.R.G., from March 28 to 30,1985. The
topics of the meeting dealt with the aspects of changes in bacterial metabolism and
structure which occur under the influence of antibiotics, and with the effects of such
changes on the antibacterial host resistance. The influence on pathogenicity factors,
changes in the outer membrane of bacteria, as well as the influence on the individual
components of the defence system were analysed in detail. In addition, these studies
showed that antibiotics proved to be an excellent tool for the examination of
bacterial physiology, so that, 50 years after the introduction of antibiotics, additional
important knowledge can be gained about the effect of these substances on bacteria.
Considering the observations reported, it appears justifiable to postulate that new
antibiotics should be routinely tested with respect to their possible effects on
antiinfectious resistance. Of course, a consensus will have to be found on which to
base methods and criteria employ€d.
The symposium documented an increasing interest of microbiologists and clini
cians for this field of research. It would not have been possible to organize it without
the substantial support of the Paul Ehrlich Society as well as of Squibb-Von Heyden
Pharma, Inc. Particular help concerning the organization has been given by Werner
Kremer of Squibb-Von Heyden Pharma. The organizing committee would like to
express its gratitude and appreciation for this financial and personal help. It also
thanks Bayer (Wuppertal), Boehringer (Mannheim), Griinenthal-Takeda
(Aachen), Merck (Darmstadt), Merck, Sharp & Dohme (Munich), Pfizer (Karls
ruhe), and Squipp-Von Heyen (Munich) for supporting the publication of these
proceedings. The editors hope that this publication will help to spread the knowl
edge and interest about these interesting topics as widely as possible.
October 1985 D. Adam
H. Hahn
w. Opferkuch
Contents
I. The Inflnence of Antibiotics on the Morphology and the Surface of
Bacteria
Effect of Subinhibitory Concentrations of Antibiotics on Surface Proteins of
Neisseria gonorrhoeae and Neisseria meningitidis:
Changes that Alter Attachment of Human Cells
D. S. STEPHENS and Z. A. MCGEE 3
Discussion. . . . . . . . . . . . . . . . . . . . 14
Interaction of Aminoglycosides and Ciprofloxacin with Bacterial Membranes
A. DALHOFF 16
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
The Influence of Ciprofloxacin on the Ultrastructure of Gram-negative and
Gram-positive Bacteria
W.-H. VOIGT. 28
Discussion . . . . . . . 35
Exposure of Protein Antigens and Reduction of Siderophore Production
after growth of Gram-Negative Bacteria in Sub-Inhibitory Concentrations of
Cephalosporins
J. L. KAOURUGAMUWA, H. ANWAR, M. R. W. BROWN, and O. ZAK 36
Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
The Influence of B-Lactam Antibiotics Including Monobactam on the outer
and inner Membrane of E. coli
H. J. LEYING, H. KARCH, H. P. KROLL, and W. OPFERKUCH. 48
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . 56
VIII Contents
Changes in outer Membrane Composition after Treatment of Proteus
mirabilis with Antibiotics
K. NIXDORFF, H. H. MARTIN, and N. SIEGMUND-SCHULTZE 57
Discussion . . . . . . . . . . . . . . . . . . . . . . . . 64
Weight and Morphology of Bacteria Exposed to Antibiotics
v. LORIAN, W. TOSCH, and D. JOYCE. 65
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . 71
II. The Influence of Antibiotics on Immunogenicity
Immunogenicity of Bacteria Treated in vitro with Subinhibitory
Concentrations of Antibiotics
H. FRIEDMAN. 75
Discussion . . . . . . . . . . 83
Influence of Subminimal Inhibitory Concentrations of Antibiotics on Antigen
Expression and Metabolic Activity of Salmonella Species
G. GILLISSEN and B. MELZER . 84
Discussion . . . . . . . . . . . . . . . . . . . . . . . . 94
Influence of Ciprofloxacin on Specific Interactions on Listeria-specific T Cells
with Antigen in vitro .
H. HAHN, B. Wos, and U. SPERLING 96
Discussion . . . . . . . . . . . . . 103
III. The Influence of Antibiotics on the Interaction of Bacteria
with Host Defence Mechanisms
a) Serum Bactericidal Activity
Effects of Antibiotics on the Sensitivity of Proteus mirabilis to the Bactericidal
Action of Normal Human Serum
N. SIEGMUND-SCHULTZE, H. H. MARTIN, and K. NIXDORFF 107
Discussion . . . . . . . . . . . . . . . . . . . . . . . . 115
Imipenem Renders Serum Resistant Enterobacter aerogenes and Escherichia
coli to Serum Sensitive Bacteria
C. WIEMER, B. KUBENS, and W. OPFERKUCH 117
Discussion . . . . . . . . . . . . . . . . . 124
Contents IX
b) Phagocytosis
Influence of Subinhibitory Concentrations of Clindamycin
on the Phagocytosis of Staphylococcus aureus
E. VERINGA and J. VERHOEF 125
Discussion . . . . . . . . . . . . . . . . . . 130
The Effect of Antibiotics on the Killing of E. Coli by Macrophages of the Cell
LineP388Dl
M. PAWELZIK, C. WIEMER, K. H. BOSCHER, and W. OPFERKUCH. 131
Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Antibiotics and Polyelectrolytes Modulate Bacteriolysis and the Capacity of
Bacteria to Trigger an Oxygen Burst in Neutrophils
I. GINSBURG, R. BORINSKI, M. SADOVNIK, S. SHAULl, J. WECKE, P. GIESBRECHT,
andM. LAHAV . 141
Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Chemiluminescence of Macrophages induced by Non-Opsonized E. coli
treated with Subinhibitory Concentrations of B-Lactam Antibiotics
K. H. BOSCHER, H. LEYING, M. PAWELZIK, V. KLiMETZEK, and W. OPFERKUCH 152
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
Indirect Antibiotic Effects on Granulocyte Migration and Phagocytosis
H. GNARPE and J. BELSHEIM 159
Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Interactions between human Polymorphonuclear Neutrophils and Ampicillin
treated Escherichia coli
L. A. MANDELL. 166
Discussion . . . . . . . 178
The Effect of Subinhibitory Concentrations of selected Antibiotics
on Bacteria-Phagocyte Interactions
W. RoszKowsKI, P. CIBOROWSKI, H. L. Ko, F. SCHUMACHER-PERDREAU,
K. ROSZKowsKI, J. JELJASZEWICZ, and G. PULVERER. 179
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
The effect of subinhibitory concentrations of monobactams and bactericidal
activity of macrophages
K. IrDA-TANAKA, T. TANAKA, S. IRINo, and A. NAGAYAMA . 188
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . 199
X Contents
IV. The Influence of Antibiotics on the Pathogenicity Factors and Virulence
a) Adherence
Effects of Antibiotics on Streptococcal Adherence: Release of Fibronectin
Lipoteichoic Acid Complexes from Group A Streptococci by Sublethal
Concentrations of Penicillin
E. H. BEACHEY, T. J. NEALON, H. S. COURTMEY, and W. A. SIMPSON 203
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 208
Influence of Subinhibitory Concentrations of Antibiotics on Aggregation and
Adherence of Streptococcus mutans
M. GHIONE, G. MEIXELSPERGER, G. PELIZZONI, and T. GLIOZZI . 209
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . 219
b) Toxin Production and Virulence
Clostridium Difficile Colonization of Infant Hamsters
R. D. ROLFE and J. P. IACONIS 220
Discussion . . . . . . . . . . 228
Gentamicin and Tobramycin selectively inhibit the secretion
of Pseudomonas aeruginosa elastase
R.L. WARREN 230
Discussion . . . . . . . . . . . . . . 238
Subinhibitory concentrations of antibiotics modulate amount of Shiga-like
toxin produced by Escherichia coli
H. KARCH, P. GORONCy-BERMES, W. OPFERKUCH, H.-P. KROLL,
and A. O'BRIEN 239
Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
Interference of ciprofloxacin with the expression of pathogenicity factors of
Pseudomonas aeruginosa
A. DALHOFF and G. DORING 246
Discussion . . . . . . . . . 255
Contents XI
V. Miscellaneous
In vivo Consequences of the Interaction between Antibiotic-damaged
Pathogens and Host Animal Defences
C. G. GEMMELL. 259
Discussion . . . . . . . . . . . . . . . 265
The Influence of Streptomycin on the Ecology of the Intestine and Resistance
to Infection
D. J. HENTGES, J. U. QUE, and S. W. CASEY. 267
Discussion . . . . . . . . . . . . . . . . . . 275
Protection of tracheal explants infected with Pseudomonas aeruginosa
by sub inhibitory concentrations of aminoglycosides
N. R. BAKER and T. GEERS. 277
Discussion . . . . . . . . . . . . . . . . . . . . . . 287
Use of Lectins to Characterize Surface Alternations of B-Lactam Resistant
mutants of Pseudomonas aeruginosa
G. STUBNER and R. MARRE. . . . . . 289
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 296
List of Contributors
ANWAR, H., Birmingham, United Kingdom
BAKER, N. R., Columbus, Ohio, USA
BEACHEY, E. H., Memphis, Tennessee, USA
BELSHEIM, J., Gavle, Sweden
BORINSKI, R., Jerusalem, Israel
BROWN, M. R. W., Birmingham, United Kingdom
BUSCHER, K. H., Bochum, Federal Republic of Germany
CASEY, S. W., Lubbock, Texas, USA
CIBOROWSKI, P., Warsaw, Poland
COURTNEY, H. S., Memphis, Tennessee, USA
DALHOFF, A., Wuppertal, Federal Republic of Germany
DORING, G., Tiibingen, Federal Republic of Germany
FRIEDMAN, H., Tampa, Florida, USA
GEERS, T., Columbus, Ohio, USA
GEMMELL, C. G., Glasgow, United Kingdom
GHIONE, M., Milano, Italy
GIESBRECHT, P., Berlin, Federal Republic of Germany
GILLISSEN, G., Aachen, Federal Republic of Germany
GINSBURG, I., Jerusalem, Israel
GLIOZZI, T., Milano, Italy
GNARPE, H., Gavle, Sweden
GORONCy-BERMES, P., Bochum, Federal Republic of Germany
HAHN, H., Berlin, Federal Republic of Germany
HENTGES, D. J., Lubbock, Texas, USA