The Future of Metabolic Engineering

406 Pages·2022·7.067 MB·English

by  Abhishek Sharma, Dhruti Amin

BIOCHEMISTRY AND MOLECULAR BIOLOGY
IN THE POST GENOMIC ERA

T F
HE UTURE OF
METABOLIC ENGINEERING

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B M
IOCHEMISTRY AND OLECULAR
B P G E
IOLOGY IN THE OST ENOMIC RA

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BIOCHEMISTRY AND MOLECULAR BIOLOGY
IN THE POST GENOMIC ERA

T F
HE UTURE OF
METABOLIC ENGINEERING

ABHISHEK SHARMA
AND
DHRUTI AMIN
EDITORS
Copyright © 2022 by Nova Science Publishers, Inc.
DOI: https://doi.org/10.52305/VEAH4499

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Published by Nova Science Publishers, Inc. † New York
Contents

Foreword ....................................................................................... vii
Pragya Tiwari
Preface ........................................................................................ xi
Chapter 1 Advances in Metabolic Engineering:
Applications of CRISPR and Beyond .......................... 1
Enuh Blaise Manga, Pınar Aytar Çelik,
Belma Nural Yaman and Ahmet Çabu
Chapter 2 Significant Role of Metabolic Engineering
in the Understanding of Cancer ................................. 33
Aarti Gayakwad, Neha P. Shukla
and Giftson J. Senapathy
Chapter 3 Genome Editing in Metabolically Engineered
Breast Cancer Cells with a Special Focus
on Nano-Therapeutic Applications ............................ 47
P. Parizad, R. Krishnamurthy
and Giftson J. Senapathy
Chapter 4 Invertebrate and Vertebrate Cell Cultures
in Bio-Therapeutics ..................................................... 75
Krutika Saurabh Abhyankar
Chapter 5 An Insight in the Trends Timeline
of Metabolic Engineering in Microbes
and Its Reflection on Future Scope.......................... 121
Ketankumar J. Panchal
vi Contents
Chapter 6 Metabolic Engineering Approach for
Optimization of Microalgae Biorefinery Using
Phaeodactylum Tricornutum as
Model Organism ........................................................ 151
Caroline Schmitz, Claudia M. Bauer
and Marcelo Maraschin
Chapter 7 Deciphering the Metabolic Adjustments of
Engineered Plants Using GC-MS:
A Typical Workflow.................................................. 197
Maneesh Lingwan
and Shyam Kumar Masakapalli
Chapter 8 Plants as a Bioreactor for Edible Vaccines:
Emerging Excellence ................................................. 215
Ashraf Y. Z. Khalifa, T. Savitha
and A. Sankaranarayanan
Chapter 9 Recent Development and Future Prospect of
Plant-Based Vaccines ................................................ 245
Bairagi Ghanshamdas R. and Vipul P. Patel
Chapter 10 Metabolic Engineering Approaches for
Plant Secondary Metabolites Biosynthesis.............. 271
Subir Kumar Bose, Ramesh Kumar
and Reshma Kapoor
Chapter 11 Plant Metabolic Engineering:
Promotion of Human Health .................................... 313
Rita Mahapatra
Chapter 12 In Silico Studies Describing MiRNAs and Their
Regularity Processes in Industrial Important
Medicinal and Aromatic Plants ............................... 355
Anand Mishra and Pankhuri Gupta
About the Editors ................................................................................ 375
Index ..................................................................................... 379
FOREWORD

Pragya Tiwari
Department of Biotechnology, Yeungnam University,
Gyeongsan, Gyeongbuk, Republic of Korea

The last two decades have witnessed increasing data on metabolic
networks in biological species, projecting a need to elucidate cellular
metabolism via scientific interventions. The existing and forthcoming
developments in genetic manipulation of biological systems have
empowered the field of metabolic engineering to explore/attempt
alterations in metabolic pathways and entire pathway reconstitution in
viii Pragya Tiwari
biological systems, putting forth the concept of “system metabolic
engineering”. The ever-expanding toolbox of “metabolic engineering”
has been benefited by the strategies to insert/delete or overexpress
targeted genes and regulation/modulation of metabolic networks,
defining new paradigms in genetic manipulation of biological systems.
The field of metabolic engineering has made huge strides in creating
“designer organisms” with desired modifications, highlighting
prospective avenues in multi-faceted applications, including agriculture,
environment, and health care. The advent of metabolic engineering in
industrial biotechnology has been a recent emerging concept that aims to
boost industrial/large-scale production of value-added metabolites,
foodstuffs, and biofuels on a commercial scale. With its initial
implementation, the science of metabolic engineering has come a long
way and witnessed new developments, while addressing the associated
challenges to improve and redirect metabolic flux towards targeted
production of the desired metabolite, however, much remains to be
addressed for realistic application of these goals. Scientific interventions
towards targeted and enhanced production of desired metabolites may be
attempted through various strategies: limiting metabolic flux towards
undesired by-products and improving precursor supply, promoting the
uptake of substrates, pathway reconstitution, and optimization of
constituent enzymes and extracellular production of targeted metabolite,
respectively. In addition, the increased understanding of the metabolic
networks in biological species has aided in altering the copy number of
genes and promoter regulation and thereby changes in tuning gene
expression, ensuring the optimal supply of co-factors and enhancing the
function of pathway enzymes.
The field of metabolic engineering has witnessed significant success
in genetic manipulation of microbial/plant systems, towards the
production of plant-based vaccines, value-added metabolites of
therapeutic/industrial importance, improving trait of biological
system/yield of targeted metabolites, environmental applications,
biofuels/smart crops in agriculture and a number of diverse applications
in present time. As “biosynthetic platforms” for targeted metabolite