The Effects of Colchicine on Metemorphosis of the Ileum and the Colon of Cules Pipiens Linn

92 Pages·4.106 MB·English

by  Schuh, Joseph E

FORDHAM UNIVERSITY
GRADUATE SCHOOL
.Max..l4j.......19.. 51
This dissertation prepared under my direction by
Joseph E. Schuhj SJ
entitled frHS EFRtCT^ COLCHICINE ON THE
METAMORPHOSIS OF THE ILEUM AND THE COLON
................OF CULES PIP TENS LJW
has been accepted in partial fulfilment of the requirements for the
Decree of Doctor 01 Philosophy
Dr. Charies .Berger... SJ
(Faculty Adviser)

THE EFFECTS OF COLCHICINE ON THE
METAMORPHOSIS OF THE ILEUM AND THE COLON
OF CULEX PIPIENS LINN
BY
JOSEPH E. SCHUH, S.J.
A.B., Georgetown University, *38
Ph.L., Woodstock College, 9 39
M.S*, Fordham University, ,5*1
S.T.L., Weston College, 9k>6
DISSERTATION
SUBMITTED IN PARTIAL FULFILLMENT OF THE
REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY
IN THE DEPARTMENT OF BIOLOGY AT
FORDHAM UNIVERSITY
NEW YORK
1951
ProQuest Number: 10992976
All rights reserved
INFORMATION TO ALL USERS
The quality of this reproduction is dependent upon the quality of the copy submitted.
In the unlikely event that the author did not send a complete manuscript
and there are missing pages, these will be noted. Also, if material had to be removed,
a note will indicate the deletion.
uest
ProQuest 10992976
Published by ProQuest LLC(2018). Copyright of the Dissertation is held by the Author.
All rights reserved.
This work is protected against unauthorized copying under Title 17, United States Code
Microform Edition © ProQuest LLC.
ProQuest LLC.
789 East Eisenhower Parkway
P.O. Box 1346
Ann Arbor, Ml 48106- 1346

ill
“i
r
TABLE OP CONTENTS
Page
LIST OP FIGURES................................... It
AC KNOWLEDGMENTS................................... ix
I. INTRODUCTION................................. 1
II. MATERIAL AND METHODS.................... 5
Culture methods ..... 5
Experimental methods................. 8
Cytological methods ............. 12
III. OBSERVATIONS AND RESULTS.................... 14
Normal metamorphosis of the hind-gut........ 14
Effects of colchicine on the metamorphosis
of the hind-gut .... ....... ................ 16
Treatment begun before metamorphosis....... 31
A. Ileum...... 31
B. Colon..........*...................... 32
Treatment begun during metamorphosis........ 33
A. Ileum.................................. 33
B. Colon............................ 48
General observations ..... 49
IV. DISCUSSION................................... 64
V. SUMMARY....................................... 74
BIBLIOGRAPHY..................................... . 76
L
iv
r
“i
LIST OF FIGrtJHES
Figure Page
1. Breeding cage and culture jars.... 7
2. Micromanipulator used in experiments 11
3* Normal ileum and colon of 2 hour pupa of
Culex pipiens. Feulgen total mount* xlOO 18
4. Normal ileum of 2 hour pupa of Culex
pipiens. Feulgen total mount. x200.... 18
5. Normal ileum and colon of 4 hour pupa of
Culex pipiens. Feulgen total mount* xlOO 20
6. Normal ileum of 4 hour pupa of Culex
pipiens. Feulgen total mount. x200..*.* 20
7. Normal ileum and colon of 6 hour pupa of
Culex pipiens* Feulgen total mount. xlOO 22
8 . Normal ileum and anterior portion of
colon of 6 hour pupa of Culex pipiens*
Feulgen total mount. x200............... 22
9. Normal hind-gut of 13 hour pupa of Culex
pipiens. Feulgen total mount. xlOO..... 24
10* Normal ileum of 13 hour pupa of Culex
pipiens. Feulgen total mount. x200..... 2k
11. Normal colon of 13 hour pupa of Culex
pipiens. Feulgen total mount. x200..... 2k
12. Normal hind-gut of 15 hour pupa of Culex
pipiens. Feulgen total mount. xlOO 26
13* Normal ileum, colon and ileo-colon of 15
hour pupa of Culex pipiens. Feulgen
total mount. x200............. J......... 26
14. Normal hind-gut of 26 hour pupa of Culex
pipiens. Feulgen total mount. xlOO..... 28
15* Normal ileo-colon of 26 hour pupa of Culex
pipiens. Feulgen total mount. x200..... 28
L
V
r -i
Figure Page
16. Hind-gut of 2 hour pupa of Culex pipiens.
treated for 23"^ hours with Buck's Ringer
solution. Control for colchicine-treated
specimen shown in figures 30 and 31.
Feulgen total mount. xlOO............... 30
17* Ileo-colon and rectum of 2 hour pupa of
Culex pipiens. treated for 23a hours with
Buck's Ringer solution. Control for col­
chicine-treated specimen shown in figures
30 and 31. Feulgen total mount. x200... 30
18. Hind-gut of % hour pupa of Culex pipiens
treated for 13# hours with .25 per cent
solution of colchicine. Feulgen total
mount. xlOO............... 35
19. Ileum and anterior portion of colon of i
hour pupa of Culex pipiens treated for 133
hours with .25 per cent colchicine solu­
tion. Feulgen total mount. x200........ 35
20. Hind-gut of 2 hour pupa of Culex pipiens
treated for 6 hours with .25 per cent so-
lution of colchicine. Feulgen total
mount. xlOO...... 37
21. Posterior portion of ileum and anterior
portion of colon of 2 hour pupa of Culex
pipiens treated for 6 hours with .25 per
cent solution of colchicine. Feulgen
total mount. x200........................ 37
22. Hind-gut of 2 hour pupa of Culex pipiens
treated for 8 hours with .25 per cent so­
lution of colchicine. Feulgen tota^.
mount. xlOO............................... 39
23. Posterior region of ileum and anterior
region of colon of 2 hour pupa of Culex
pipiens treated for 8 hours with .25 per
cent solution of colchicine.Feulgen total
mount. x200......... 39
2^. Hind-gut of 2 hour pupa of Culex pipiens
treated for 9 hours with .25 per cent so­
lution of colchicine. Feulgen total
mount. xlOO............................... 41
L J
vi
r 1
Figure Page
25. Hind-gut of 2 hour pupa of Culex pipiens
treated for 9 hours with .25 per cent
solution of colchicine. Feulgen total
mount. x200............................. ^1
26. Hind-gut of 2 hour pupa of Culex pipiens
treated for 12 hours with .25 per cent
solution of colchicine. Feulgen total
mount. xlOO............................ . 4 3
27* Hind-gut of 2 hour pupa of Culex pipiens
treated for 12 hours with .25 per cent
solution of colchicine. Feulgen total
mount. x200......... ^3
28. Hind-gut of 2 hour pupa of Culex pipiens
treated for 1^ hours with .25 per cent
solution of colchicine. Feulgen total
mount. xlOO........ ^5
29# Ileum and anterior region of colon of 2
hour pupa of Culex pipiens treated for l^f
hours with .25 per cent solution of col­
chicine. Feulgen total mount. x200.... ^5
30. Anterior portion of ileum of 2 hour pupa
of Culex pipiens treated for 233 hours
with .25 per cent solution of colchicine.
Feulgen total mount. xlOO............... ^7
31. Anterior region of ileum of 2 hour pupa
of Culex pipiens treated for 23'^ hours
with .25 per cent solution of colchicine.
Feulgen total mount. x200............. ^7
32. Hind-gut of ki hour pupa of Culex pipiens
treated for 13 hours wTith .25 per cent
solution of colchicine. Feulgen total
mount. xlOO.............................. 51
33. Ileum of pupa of ki hour pupa of Culex
pipiens treated for 13 hours with .25 per
cent solution of colchicine. Feulgen
total mount. x200..... 51
3^. Hind-gut of 8 hour pupa of Culex pipiens
treated for 6 hours with .25 per cent so-
lution of colchicine. Feulgen total
mount. xlOO............................ 53
L -I
vii
r n
Figure Page
35• Ileum of 8 hour pupa of Culex pipiens
treated for 6 hours with .25 per cent
solution of colchicine. Feulgen total
mount. x200........................... 53
36. Colon of 8 hour pupa of Culex pipiens
treated for 6 hours with .25 per cent
solution of colchicine. Feulgen total
mount. x200................. 53
37* Hind-gut of 12 hour pupa of Culex
pipiens treated for 1 hour with .25
per cent solution of colchicine.
Feulgen total mount. xlOO............ 55
38. Ileum of 12 hour pupa of Culex pipiens
treated for 1 hour with .25 per cent
solution of colchicine. Feulgen total
mount. x200........................... 55
39• Hind-gut of 12 hour pupa of Culex
pipiens treated for 2 hours with .25
per cent solution of colchicine.
Feulgen total mount. xlOO. . ... 57
40. Ileum of 12 hour pupa of Culex pipiens
treated for 2 hours with .25 per cent
solution of colchicine. Feulgen total
mount. x200.............. 57
41. Hind-gut of 13 hour pupa of Culex
pipiens treated for 1 hour with .25
per cent solution of colchicine.
Feulgen total mount. xlOO........... 59
42. Ileum of 13 hour pupa of Culex pipiens
treated for 1 hour with .25 per cent
solution of colchicine. Feulgen total
mount. x200........................... 59
43. Ileo-colon of 13 hour pupa of Culex
pipiens treated for 1 hour with .25
per cent solution of colchicine.
Feulgen total mount. x200............. 59
44. Hind-gut of 13 hour pupa of Culex
pipiens treated for 3 hours with .25
per cent solution of colchicine.
Feulgen total mount. xlOO............. 61
L j