The Biology of DNA Tumor Viruses: With an Introduction by Renato Dulbecco

265 Pages·1976·10.19 MB·English

by  R. Dulbecco (auth.), Samuel Schiminovich (eds.)

The Biology of
DNA Tumor Viruses
With an Introduction by Renata Oulbecco
Milestones in Current Research
Collections of Influential Contributions in Key Areas of Science
====== ======
Series Editor: Samuel Schiminovich
Volume 1: THE BIOLOGY OF DNA TUMOR VIRUSES
With an Introduction by Renato Dulbecco
The Biology of
Tumor Viruses
DNA
With an Introduction by Renato Dulbecco
Edited by
Samuel Schiminovich
Springer Science+Business Media, LLC
library of Congress Cataloging in Publication Data
Main entry under title:
The Biology of DNA tumor viruses.
l'
(Milestones in current research; v.
Includes index.
1. Oncogenic viruses. I. Schiminovich, Samuel. II. Title: DNA tumor viruses.
[DNLM: 1. Oncogenic viruses. 2. Polyoma virus. 3. SV40 virus. 4. DNA, neoplasm.
aZ202 B616)
RC268.57.B56 616.9'92'0194 76-25874
ISBN 978-1-4684-6972-1 ISBN 978-1-4684-6970-7 (eBook)
DOI 10.1007/978-1-4684-6970-7
© 1976 Springer Science+Business Media New York
Originally published by Plenum Press, New York in 1976
Softcover reprint of the hardcover 1st edition 1976
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y. 10011
All Rights Reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted,
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
ACKNOWLEDGMENTS
The permission of the copyright owners to reprint the following ma
terial is gratefully acknowledged.
Aloni, Y., Winocour, E., and Sachs, L., Characterization of the simian virus 40-specific RNA in virus
yielding and transformed cells, Journal of Molecular Biology, 1968,31:415-429.
Benjamin, T.L., Virus-specific RNA in cells productively infected or transformed by polyoma virus,
Journal of Molecular Biology, 1966, 16:359-373.
Black, P.H., Rowe, W.P., TUrner, H.C., and Huebner, R.J., A specific complement-fixing antigen pres
ent in SV40 tumor and transformed cells, Proceedings of the National Academy of Sciences, 1963,
50: 1148-1156.
Dulbecco, R., and Eckhart, W., Temperature-dependent properties of cells transformed by a thermo
sensitive mutant of polyoma virus, Proceedings of the National Academy of Sciences, 1970,67:
1775-1781.
Dulbecco, R., Hartwell, L.H., and Vogt, M., Induction of cellular DNA synthesis by polyoma virus,
Proceedings of the National Academy of Sciences, 1965,53:403-410.
Dulbecco, R., and Stoker, M., Abortive transformation by the Tsa mutant of polyoma virus, Nature,
1969,223:397-398.
Dulbecco, R., and Vogt, M., Evidence for a ring structure of polyoma virus DNA, Proceedings of the
National Academy of Sciences, 1963,50:236-243.
Fried, M., Cell-transforming ability of a temperature-sensitive mutant of polyoma virus, Proceedings of
the National Academy of Sciences, 1965, 53:486-491.
Gerber, P., Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to
indicator cells, Virology, 1966,28:501-509.
Khoury, G., Martin, M.A., Lee, T.N.H., Danna, K.J., and Nathans, D., A map of simian virus 40 tran
scription sites expressed in productively infected cells, Journal of Molecular Biology, 1973, 78:
377-389.
Koprowski, H., Jensen, F.C., and Steplewski, Z., Activation of production of infectious tumor virus
SV40 in heterokaryon cultures, Proceedings of the National Academy of Sciences, 1967,58:127-
133.
Lindstrom, D.M., and Dulbecco, R., Strand orientation of simian virus 40 transcription in productive
ly infected cells, Proceedings of the National Academy of Sciences, 1972, 69: 1517-1520.
Oda, K., and Dulbecco, R., Regulation of transcription of the SV 40 DNA in productively infected and
in transformed cells, Proceedings of the National Academy of Sciences, 1968,60:525-532.
Pollack, R.E., Green, H., and Todaro, G.J., Growth control in cultured cells: Selection of sublines with
increased sensitivity to contact inhibition and decreased tumor-producing ability, Proceedings of
the National Academy of Sciences, 1968,60: 126-133.
Pope, J.H., and Rowe, W.P., Detection of specific antigen in SV40-transformed cells by immunofluo
rescence, The Journal of Experimental Medicine, 1964, 120: 121-128.
Radloff, R., Bauer, W., and Vinograd, J., A dye-buoyant-density method for the detection and isola
tion of closed circular duplex DNA: The closed circular DNA in He La cells, Proceedings of the Na
tional Academy of Sciences, 1967,57:1514-1521.
Rapp, F., Kitahara, T., Butel, J.S., and Melnick, J.L., Synthesis of SV40 tumor antigen during replica
tion of simian papovavirus (SV40), Proceedings of the National Academy of Sciences, 1964, 52:
1138-1142.
Renger, H.C., and Basilico, C., Mutation causing temperature-sensitive expression of cell transforma
tion by a tumor virus, Proceedings of the National Academy of Sciences, 1972, 69: 109-114.
Sambrook, J., Westphal, H., Srinivasan, P.R., and Dulbecco, R., The integrated state of viral DNA in
SV40-transformed cells, Proceedings of the National Academy of Sciences, 1968,60:1288-1295.
Sauer, G., and Kidwai, J.R., The transcription of the SV40 genome in productively infected and trans
formed cells, Proceedings of the National Academy of Sciences, 1968,61:1256-1263.
Stoker, M., Abortive transformation by polyoma virus, Nature, 1968,218:234-238.
Vinograd, J., Lebowitz, J., Radloff, R., Watson, R., and Paipis, P., The twisted circular form of poly
oma viral DNA, Proceedings of the National Academy of Sciences, 1965,53:1104-1111.
Wall, R., and Darnell, J.E., Presence of cell and virus specific sequences in the same molecules of nu
clear RNA from virus transformed cells, Nature New Biology, 1971,232:73-76.
Watkins, J.F., and Dulbecco, R., Production of SV 40 virus in heterokaryons of transformed and suscep
tible cells, Proceedings (,f the National Academy of Sciences, 1967,58: 1396-1403.
Weil, R., and Vinograd, J., The cyclic helix and cyclic coil forms of polyoma viral DNA, Proceedings of
the National Academy of Sciences, 1963,50:730-738.
Westphal, H., and Dulbecco, R., Viral DNA in polyoma-and SV40-transformed cell lines, Proceedings
of the National Academy of Sciences, 1968,59:1158-1165.
FOREWORD
Milestones in Current Research is a series of reprint collections dis
tinguished from other such publications by new concepts in preparation,
presentation, and intent. The aim of each volume is to gather for a given field
the seminal contributions that have defined and shaped the trends within the
most active areas of current research. The selections for each volume and the
structure of the book have been determined with the help of a novel tech
nique of bibliographic analysis and have then been presented to an acknowl
edged scientific authority for minor adjustments and the writing of an In
troduction. These introductions will lend historic perspective to the material
selected for each volume. The bibliographic analysis used tends to select
papers central to the areas of current research within, roughly, the last
decade and is a systematic procedure for depicting, delineating, and covering
all such areas over a wide spectrum of scientific research.
It is hoped that with this procedure it will be possible to achieve an
objectivity, authority, and thoroughness not reached by others and that the
timeliness of the volumes will not be limited to just a few years. Each volume
should have the permanent value of a historical statement and yet be suf
ficiently interesting to active researchers in the field as well as to students
exploring the quiet way in which the relentless drama of research unfolds in
the journal literature. Furthermore, follow-up volumes will periodically up
date the status of research and maintain the currency of the series as a whole.
While the series may well become a prestige forum where an outstanding
segment of the journal literature is reprinted, readers may occasionally miss a
paper that they would expect to be included. Such omissions are bound to
occur for a number of reasons, including among others the need to keep the
volumes to a viable size or the sheer unavailability of the given paper for re
production due to unusual copyright restrictions.
Our gratitude is extended to all those who in one way or another have
helped us to bring these concepts to fruition, in particular to Professors
vii
viii FOREWORD
Ralph Schiller and Howard M. Temin for their enlightening insights and early
encouragement, to all copyright holders who have made their articles available
for reproduction, and to Dr. Renato Dulbecco for making possible such an
auspicious start for the series.
Samuel Schiminovich
Series Editor
CONTENTS
I. Introduction ......................... R. Dulbecco 3
II. Structure of Viral DNA .......................... 11
1. Evidence for a Ring Structure of Polyoma
Virus DNA ............ R. Dulbecco and M. Vogt 13
2. The Cyclic Helix and Cyclic Coil Forms
of Polyoma Viral DNA .... R. Weil and J. Vinograd 21
3. The Twisted Circular Form of Polyoma
Viral DNA ............. J. Vinograd, J. Lebowitz,
R. Radloff, R. Watson, and P. Laipis 31
4. A Dye-Buoyant-Density Method
for the Detection and Isolation of Closed
Circular Duplex DNA: The Closed Circular
DNA in HeLa Cells ...... R. Radloff, W. Bauer,
and J. Vinograd 39
III. Integration State of Viral DNA in Transfonned Cells ... 47
1. Viral DNA in Polyoma- and SV40-Transformed
Cell Lines .......... H. Westphal and R. Dulbecco 49
2. The Integrated State of Viral DNA
in SV40-Transformed Cells ......... J. Sambrook,
H. Westphal, P.R. Srinivasan, and R. Dulbecco 57
IV. Detection of Virus-Specific (T) Antigen ............. 65
1. A Specific Complement-Fixing Antigen
Present in SV40 Tumor and Transformed
Cells ................. P.H. Black, W.P. Rowe,
H.C. Turner, and R.J. Huebner 67
2. Detection of Specific Antigen in SV40-
Transformed Cells by Immunofluorescence ........
J.H. Pope and W.P. Rowe 77
ix
x CONTENTS
3. Synthesis of SV40 Tumor Antigen
during Replication of Simian Papovavirus
(SV40) ................. F. Rapp, T. Kitahara,
J .S. Butel, and J.L. Melnick 85
V. Activation of Infectious Tumor Virus SV 40
in Transformed Cells .......................... 89
1. Studies on the Transfer of Subviral
Infectivity from SV 40-Induced Hamster
Tumor Cells to Indicator Cells ........ P. Gerber 91
2. Activation of Production of Infectious
Tumor Virus SV 40 in Heterokaryon
Cultures ........... H. Koprowski, F.e. Jensen,
and Z. Steplewski 101
3. Production of SV 40 Virus in Heterokaryons
of Transformed and Susceptible Cells .. J.F. Watkins
and R. Dulbecco 109
VI. Transcription of the Viral Genome
In Infected and Transformed Cells ................ 117
1. Virus-Specific RNA in Cells Productively
Infected or Transformed by Polyoma Virus .......
T.L. Benjamin 119
2. Characterization of the Simian Virus 40-specific
RNA in Virus-yielding and Transformed Cells ......
Y. Aloni, E. Winocour, and L. Sachs 135
3. Regulation of Transcription of the SV40
DNA in Productively Infected and in
Transformed Cells ......K . Oda and R. Dulbecco 151
4. The Transcription of the SV 40 Genome
in Productively Infected and Transformed Cells ....
G. Sauer and J.R. Kidwai 159
5. Strand Orientation of Simian Virus 40
Transcription in Productively Infected Cells .......
D.M. Lindstrom and R. Dulbecco 167
6. A Map of Simian Virus 40 Transcription
Sites Expressed in Productively Infected Cells ......
G. Khoury, M.A. Martin, T.N.H. Lee, K.J. Danna,
and D. Nathans 177
7. Presence of Cell and Virus Specific
Sequences in the Same Molecules of Nuclear
RNA from Virus Transformed Cells ...... R. Wall
and J.E. Darnell 191