th The 9 International Symposium of the Microbial Ecology of Aerial Plant Surfaces August 15‐18, 2010 Oregon State University, Corvallis, OR Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces Organizing Committee Convener and local arrangements Walt Mahaffee USDA Agriculture Research Service, Corvallis, Oregon and Department of Botany and Plant Pathology, Oregon State University Scientific Program Committee Maria Brandl USDA Agriculture Research Service, Albany, California Gwyn A. Beattie Iowa State Univ, Plant Pathology Dept, Ames, Iowa Barry J. Jacobsen Montana State Univ, Plant Sciences & Plant Pathology Dept, Bozeman, Montana Johan Leveau Department of Plant Pathology, University California, Davis Steve Lindow Department of Plant and Microbial Biology, University California, Berkley Cindy E. Morris INRA, Unite de Pathologie Végétale, Montfavet – France Vicky Toussaint Agriculture and Agri-Food Canada, Horticulture Research and Development Centre, Quebec, Canada Julia Vorholt Institute of. Microbiology, Swiss Federal Institutes of Technology in Zurich, Zurich, Switzerland Special Thanks to: Maya Perez and Carly Weber of OSU Conference Services for web site, site planning, and registration. 2 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces Table of Contents Maps………………………………………………………………………………………………………………….. 4 Program Schedule……………………………………………………………………………………………… 6 Oral Abstracts (in order of presentation)…………….…………………………………………….. 13 Poster Abstracts………………………………………………..………………………………………………. 63 Directory of Participants……………………………………………………………………………………. 88 Sponsors United States Department of Agriculture Agriculture Research Service Steve Lindow Family Fund Pacific Gas and Electric Agriculture and Agriculture et Agri-Food Canada Agroalimentaire Canada Tuesday’s Wines Contributed by: Annie Amie Winery Lumos Winery Argyle Winery Soter Vineyards Chehalen Winery Willamette Valley Winery Lemelson Winery 3 | P a g e Downtown Corvallis 1 mile Numerous restaurants, shops and bars Avery Park Bloss Hall Weatherford Hilton Garden Inn Hall LaSells Stewart Center Oregon State University Campus Map Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces Oral Presentations Meals, Breaks, and posters LaSells Stewart Center 5 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces Program Schedule LaSells Stewart Center, Construction and Engineering Hall Sunday Aug 15 7:00 AM Hosted Breakfast ‐ Giustina Gallery 8:00 AM Meeting opening ‐ Construction and Engineering Hall Interactions between the phyllosphere and the atmosphere Moderator: Steve Lindow, Department of Plant and Microbial Biology, University California, Berkley 8:15 AM Mapping the world’s VOC emissions Christine Wiedinmyer National Center for Atmospheric Research, Boulder, Colorado 8:55 AM Plants as sources of micro‐organisms for clouds – mechanisms and processes P. Amato1,2,*, Leyronas, C.2, D. Courault1 and C. Morris2 1INRA, UMR1114 EMMAH, Avignon, France; 2INRA, UR407 Plant Pathology Unit, Montfavet, France: * Now at SEESIB – UMR6504 CNRS‐Clermont Université 9:35 AM Epidemic waves and dispersal of phyllosphere microorganisms Christ Mundt Oregon State University, Dept. Botany and Plant Pathology 10:15 AM Break ‐ Giustina Gallery 10:30 AM Modeling the dispersion and deposition of microorganisms in sparse canopies Rob Stoll1, Walt Mahaffee2 and Eric Pardyjak1 1Dept Mechanical Engineering, University of Utah, Salt Lake, UT; 2Hort Crops Research Lab, USDA‐ARS and Dept. of Botany and Plant Pathology, Oregon State University, Corvallis, OR 11:15 AM Trichomes and structured epicuticular waxes on plant surfaces – an optimality approach to the prevalent aerosol regimes? J. Burkhardt, M. Hunsche, S. Pariyar University of Bonn, Institute of Crop Science and Ressource, Bonn Germany Ecological processes dependent on the open habitat of the phyllosphere Moderator: Johan Leveau, Department of Plant Pathology, University California, Davis 11:30 AM A bacterial effector manipulates the host phenotype to enhance insect vector reproduction A. Sugio, H. N. Kingdom, V. M. Nicholls and S. A. Hogenhout The John Innes Centre, Colney Lane, Norwich Research Park, Norwich, NR4 7UH, UK. 6 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces 12:10 PM Hosted lunch ‐ Giustina Gallery 1:10 AM An archetypical epiphyte in the hydrosphere: Biogeography of Pseudomonas syringae in river source waters. Cindy E. Morris1,2 and David C. Sands2 1INRA, UR407 Pathologie Végétale, Montfavet, France; 2Dept. Plant Sciences and Plant Pathology, Montana State University 1:50 PM Insect vectoring of phyllosphere microbes J.L. Shipp1, P.G. Kevan2, J.C. Sutton2, J.P. Kapongo1, .S. Al‐Mazra’Awi3, A.B. Broadbent4 & S. Khosla5 1Agriculture and Agri‐Food Canada, Greenhouse and Processing Crops Research Centre, Harrow, Ontario, Canada; 2Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada; 3Biotechnology Department, Al‐balqa’ Applied University, Assalt, Jordan; 4Agriculture and Agri‐Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, Canada ; 5Ontario Ministry of Agriculture, Food and Rural Affairs, Harrow, Ontario, Canada 2:30 PM The effects of species diversity and propagule density on fungal establishment and coexistence in the phyllosphere Shannon S. Nix Dept. of Biology, Clarion University, Clarion, PA 3:10 PM Break ‐ Giustina Gallery 3:25 PM Multi‐trophic level interactions on the grass phyllosphere: Fungal endophytes confer selection for a specific alkaloid toxin‐consuming bacterial microflora Elizabeth Roberts and Steven Lindow Department of Plant and Microbial Biology, University of California, Berkeley 4:05 PM Biodegradation and stress in the phyllosphere Tanja R. Scheublin1, Johan H.J. Leveau1,2 1 Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO‐KNAW), Heteren, The Netherlands 4:20 PM Impact of site and plant species in structuring Methylobacterium communities in the phyllosphere Claudia Knief, Alban Ramette, Julia Vorholt Institute of Microbiology, ETH Zurich 4:35 PM Population dynamics of Pseudomas syringae in leaf litter and snow pack in the southern french Alps Caroline L. Monteil1, Caroline Guiland1, Catherine Glaux1, François Lafolie2 and Cindy E. Morris1 1INRA, Unité de Pathologie Végétale, Domaine St Maurice, Montfavet cedex; 2INRA, Unité Climat‐ Sol‐Environnement, Domaine Saint‐Paul, Site Agroparc, 84914 Avignon, France. 4:50 PM Poster Session ‐ Giustina Gallery 5:45 PM Break 6:00 PM Board buses in front of LaSells Stewart Center *Make sure to bring a jacket 6:30 PM Hosted Dinner at Tyee Winery 9:00 PM 7 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces Monday 16 August 7:00 AM Hosted Breakfast ‐ Giustina Gallery Managing Phyllosphere Microorganisms for Plant Health and Food Safety Moderator: Maria Brandl, USDA Agriculture Research Service, Albany, California 8:00 AM Risks of contamination by human pathogens and challenges for food safety Trevor Suslow University of California, Davis 8:40 AM Treats and Tricks: Adaptation of Salmonella to the phyllosphere Yulia Kroupitski,1 Dana Golberg,1 Eduard Belausov,2 Riky Pinto,1 Dvora Swartzberg,3David Granot,3 and Shlomo Sela1 1Microbial Food‐Safety Research Unit, Department of Food Quality & Safety, Institute for Postharvest and Food Sciences, 2 Confocal Microscopy Unit, and 3 Institute for Plant Sciences, Agricultural Research Organization, The Volcani Center, Israel 9:20 AM Interactions of Microbial Pathogens of Insects and Mites with the Phyllosphere Donald C. Steinkraus Dept. of Entomology, University of Arkansas, Fayetteville, AR 10:00 AM Break ‐ Giustina Gallery 10:15 AM Existence of Salmonella Typhimurium on growing leafy greens as dictated by level of water contamination, irrigation method and type of produce Kisluk G, Yaron S. Faculty of Biotechnology and Food Engineering, Technion‐Israel Institute of Technology, Haifa, Israel 10:30 AM The human pathogen Salmonella Typhimurium inhibits tobacco immune response Natali Shirron and Sima Yaron Department of Biotechnology and Food Engineering, Technion, Haifa, Israel 10:45 AM Relevance of the phyllosphere microbiota to E. coli O157:H7 persistence on lettuce Tom Williams1, Anne‐Laure Moyne1,2, Linda J Harris1,2, and Maria L Marco1 1 Food Science & Technology, University of California, Davis;2 Western Center for Food Safety, Davis, California 11:00 AM Leaf Surface Interactions between Phakopsora pachyrhizi, the Soybean Rust Pathogen, and the Mycoparasite Simplicillium lanosoniveum N. A. Ward, R. W. Schneider, M. C. Aime, and C. L. Robertson Department of Plant Pathology and Crop Physiology,Louisiana State University Agricultural Center, Baton Rouge, LA 11:15 AM Phylloplane microfungal metabolites induce systemic acquired resistance in Hordeum vulgare var. jagriti against Drechslera graminea Bulbul Khare, Joyeeta Mitra, V.Bhuvaneswari and P.K.Paul Amity Institute of Biotechnology; Amity University, Uttar Pradesh, Noida, Uttarpradesh, India 11:30 AM Impact of the in vitro highly effective peptide antibiotic APV found in Pantoea agglomerans 48b/90 on the biocontrol of bacterial plant pathogens Ulrike Sammer1, Dieter Spiteller2 and Beate Völksch1 Institute of Microbiology, Microbial Phytopathology, Neugasse 25, Jena 8 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces 11:45 AM Analysis of the surface properties of wheat spikelet components and their role in colonization by the biocontrol antagonist Cyptococcus flavescens OH 182.9. Christopher A. Dunlap* and David A. Schisler Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture 12:00 PM The development and architecture of biofilms formed by the biocontrol agent Burkholderia pyrocinnia FP62 on Geranium. Patricia Wallace1,Tara Neil2, Bruce Arey3, and Walter Mahaffee2 1Dept. Botany and Plant Pathology, Oregon State University, Corvallis, OR; 2USDA‐ARS Horticulture Crops Research Lab, Corvallis, OR; 3Pacific Northwest National Laboratory‐Environmental Molecular Sciences Laboratory, Richland, WA 12:15 PM Phyllo‐ecology of virginia and california grown tomatoes Ottesen A. R., Strain E. A., Brown E.A. Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland 12:30 PM Pick up boxed lunch and board buses 1:00 PM Depart for Oregon Coast , includes hosted dinner *Make sure to bring a comfortable pair of walking shoes and a jacket or sweater 10:00 PM Tuesday 17 August 7:00 AM Hosted Breakfast ‐ Giustina Gallery Impact of the plant as a habitat on the phyllosphere microflora and their ecological processes Moderator: Cindy Morris, 1INRA, Unité de Pathologie Végétale, France 8:00 AM Host effects on the composition of the microbial community Ann E. Stapleton, Peter Balint‐Kurti, Susan J. Simmons, James E. Blum, Carlos L. Ballaré Department of Biology and Marine Biology, University of North Carolina at Wilmington 8:40 AM Microbial populations of the Tamarix phyllosphere: some like it hot, dry, and saline Omri M. Finkel1, Adrien Y. Burch2, Steven E. Lindow2, Anton F. Post3 and Shimshon Belkin1 1Department of Plant and Environmental Sciences, Hebrew University of Jerusalem, Edmond J Safra Campus, Givat Ram, Jerusalem, Israel; 2Dept of Plant and Microbial Sciences, University of California‐Berkeley; 3Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biology Laboratory, Woods Hole, MA, USA 9:20 AM Solute permeability of the plant cuticle and its significance for epiphyllic microorganisms Lukas Schreiber Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D‐53115 Bonn, Germany 10:00 AM Break ‐ Giustina Gallery 9 | P a g e Phyllosphere 2010: Ninth International Symposium of the Microbial Ecology of Aerial Plant Surfaces 10:15 AM Influence of the plant as a reservoir of airborne pollutants and quaternary ammonium compounds on phyllosphere microbes Chiliang Chen, Amarjyoti Sandhu, Dana R. McKeever and Gwyn A. Beattie Dept of Plant Pathology, Iowa State University, Ames, IA, USA 10:55 AM Genomic diversity of biocontrol strains of Pseudomonas spp. isolated from plant surfaces Joyce Loper1, Ed Davis1, Kent Lim2, Karl Hassan2, Sasha Tetu2, Neil Wilson2, Sierra Hartney1, Brenda Shaffer1, Virginia Stockwell1, Marcella Henkels1, Kedy Shen1, Rachel Blumhagen1, Dmitri Mavrodi3, Judith van Mortel4, Chunxu Song4, Diana Radune11, Jessica Hostetler11, Daniel Kluepfel5, Patrick Wechter6, Anne Anderson7, Young Cheol Kim8, Leland Pierson III9, Elizabeth Pierson9, Steve Lindow10, Jos Raaijmakers4, David Weller3, Linda Thomashow3, Andrew Allen11, and Ian Paulsen2 1USDA‐ARS and Oregon State University, USA; 2Macquarie University, Australia; 3USDA‐ARS and Washington State University, USA; 4Wageningen University, The Netherlands; 5USDA‐ARS, Davis, CA, USA; 6USDA‐ARS, Charleston, SC, USA; 7Utah State University, USA; 8Chonnam National University, Korea; 9Texas A&M University, USA; 10University of California at Berkeley, USA; 11J. Craig Venter Institute, USA. 11:10 AM Adaptations of lactic acid bacteria to plants Henry Low, Thomas Williams, Sybille Tachon, Benjamin Golomb and Maria L Marco Food Science & Technology, University of California, Davis 11:25 AM Very‐long chain aldehydes promote prepenetration processes of Blumeria graminis in a dose and chain‐length dependent manner. Anton Hansjakob, Markus Riederer, Ulrich Hildebrandt University of Wuerzburg, Julius‐von‐Sachs‐Institute for Biosciences, Dept. Botany II 11:40 AM The cusper bioreporter ‐ measuring reproductive success of individual bacterial in simple and complex environments Mitja N. P. Remus‐Emsermann1 and Johan H. J. Leveau1,2 1NIOO‐KNAW, 6666 GA Heteren, The Netherlands; 2UC Davis, CA, USA 11:55 PM Hosted lunch ‐ Giustina Gallery 1:00 PM Explaining bacterial patterns in the phyllosphere: a modeling approach Annemieke van der Wal1, Robin Tecon1,2, Jan‐Ulrich Kreft3, Wolf Mooij4, Mitja Remus‐ Emsermann1 and Johan Leveau1,2 1NIOO‐KNAW, 6666 GA Heteren, The Netherlands;2UC Davis, CA, USA; 3University of Birmingham, Birmingham B15 2TT, UK; 4NIOO‐KNAW, AC Nieuwersluis, The Netherlands 1:15 PM Comparative phylogenomics of phyllosphere associated Escherichia coli Isolates Guillaume MERIC, Elizabeth J. SAGGERS, Tim F. BROCKLEHURST1 Sacha LUCCHINI Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom 1:30 PM Bacterial communities in the phyllosphere of trees are associated with plant phylogeny M.R. Lambais1 and D.E. Crowley2 1Department of Soil Science, University of São Paulo, Piracicaba, SP, Brazil. 2Department of Environmental Sciences, University of Cali 1:45 PM Bacterial growth in the apoplast is limited by nutrient availability María Eugenia RAMOS, Steve LINDOW Plant and Microbial Biology Department. University of California, Berkeley. USA 2:00 PM Environmental influence on motility in Pseudomonas syringae pv. syringae B728a and implications for plant‐microbe interactions. Kevin Hockett and Steven Lindow Plant and Microbial Biology Department. University of California, Berkeley. USA 10 | P a g e
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