Systems Biology Microscopy Workshop Si l LT itt d Li ht Simple Lenses, Transmitted Light Optical ... PDF
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McGill University Life Sciences Complex Imaging Facility Systems Biology Microscopy Workshop th Tuesday December 7 , 2010 Simple Lenses, Transmitted Light Optical Train, Köhler Illumination What Does a Microscope Need To Do? Three things need to be accomplished: 1. M A G N I F Y Produce a magnified image of the specimen. 2. C O N T R A S T Render the details within the specimen visible to the imaging device: e.g. eye, camera, photomultiplier tube 3. R E S O L V E Distinguish between objects or features within the specimen. All three things need to be accomplished. Magnification and Contrast – but Low Resolution http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Image-Gallery.html Magnification and Resolution – but Low Contrast http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Image-Gallery.html Contrast and Resolution - but Low Magnification http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Image-Gallery.html Magnification, Contrast, Resolution http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Image-Gallery.html Magnification Magnify: Produce a magnified image of the specimen. Magnification = Mag. Objective lens x Mag. Eyepiece Lens 20x lens X 10x eyepiece = 200x magnification 60x lens X 10x eyepiece = 600x magnification http://media.thestar.topscms.com/images/3a/ 7d/fe044496470cbcd585a2307b6ab0.jpeg http://www.astrographics.com/ http://www5.pbrc.hawaii.edu/microangela/mhflyo.jpg GalleryPrints/Display/GP2129.jpg Simple Biconvex Lens Modified by Aleks Spurmanis from http://www.olympusmicro.com/primer/anatomy/magnification.html Simple Biconvex Lens Focal Points: Rear Focal Point (F’): Point where light coming from an infinite distance is focused to a fine point. Modified by Aleks Spurmanis from http://www.olympusmicro.com/primer/anatomy/magnification.html Simple Biconvex Lens Focal Points: Rear Focal Point (F’): Point where light coming from an infinite distance is focused to a fine point. Front Focal Point (F’): Point where light rays collected from a single point source spread out into parallel rays. Modified by Aleks Spurmanis from http://www.olympusmicro.com/primer/anatomy/magnification.html
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