Table Of Content

Historic, Archive Document Do not assume content reflects current scientific knowledge, policies, or practices. SUGARBEET RESEARCH 2004 REPORT FOREWARD SUGARBEET RESEARCH is an annual compilation of progress reports concerning research by U.S. Department of Agriculture, Agricultural Research Service investigators and other cooperators who are engaged in sugarbeet research. The report was assembled and produced at the expense of the Beet Sugar Development Foundation, and is for the sole use of its members and the cooperators. Much of the data has not been sufficiently confirmed to justify general release and interpretations may be modified with additional experimentation. This report is not intended for publication and should not be used for cited reference nor quoted in publicity or advertising. Reproduction of any portion of the material contained herein will not be permitted without the specific consent of the contributor and the Beet Sugar Development Foundation. The report presents results of investigations strengthened by contributions received under Cooperative Agreement between the USD A Agricultural Research Service and the Beet Sugar Development Foundation, along with the California Beet Growers Association, the Western Joint Research Committee, and the Sugarbeet and Education Board of Minnesota and North Dakota. Trade names occur in this report solely to provide specific information and do not signify endorsement by the U.S. Department of Agriculture, the Beet Sugar Development Foundation or any of the cooperating organizations. CONTENTS PAGE SECTION A - SALINAS, CALIFORNIA Contents A1 Abstracts of Papers, 2003 A3 PROJECT 261 Study of New Pathotypes of Rhizomania in the United States A19 PROJECT 221 Ecology of Beet curly top virus (BCTV) and identification of sources of resistance to BCTV for bioremediation or genetic engineering A26 PROJECTS 211 and 215 Development of Sugarbeet Breeding Lines and Germplasm A36 Index of Variety Trails A50 SECTION B - FORT COLLINS, COLORADO Contents B1 Publications B2 Notice of Release of FC720, FC722 and FC722CMS Monogerm Sugarbeet Germplasm B4 PROJECT 903 Evaluation of Contributed Lines for Resistance to Rhizoctonia solani, A Causal Fungus of Cercospora Leaf Spot B7 PROJECT 904 Evaluation of Contributed Lines for Resistance to Cercospora Beticola, A Causal Fungus of Cercospora Leaf Spot B9 CONTENTS PAGE PROJECT 420 Screening Biological Control Agents for Rhizoctonia solani Control on Sugar Beets B11 PROJECT 421 Variability in Fusarium oxysporum from Sugar Beets in the United States B15 PROJECT 440 Rhizoctonia Root Rot Resistance and Development of Genetic Resistance in Sugarbeet B18 PROJECT 441 Cercospora Leaf Spot Research and Breeding for Cercospora and Curly Top Resistance B29 PROJECT 443 Pre-Breeding: The Introgression of New Sources of Cercospora Leaf Spot Resistance from Beta Vulgaris spp.Maritima and Other Exotic Sources into Sugarbeet-Type Populations B39 SECTION C - FARGO, NORTH DAKOTA Contents C1 Publications C2 PROJECT 620 Polymerase Chain Reaction (PCR)-Based Detection of Fungal Pathogens Using Actin Gene Sequences C6 CONTENTS PAGE PROJECT 621 Mechanisms of Resistance in Sugarbeet to Fungal and Bacterial Pathogens C8 PROJECT 622 Tagging of Genes for Disease Resistance in Sugarbeet Using Molecular Genetic Markers C10 PROJECT 650 Role of Sucrose Metabolizing Enzymes in Sugarbeet Root Growth, Carbohydrate Partitioning and Postharvest Sucrose Loss Cl2 PROJECT 651 Characterization of Raffinose Biosynthesis During Sugarbeet Production and Storage Cl7 SECTION D - EAST LANSING, MICHIGAN Contents D1 Manuscripts Published D3 Sugarbeet Activities Conducted in Cooperation with Saginaw Valley Bean and Beet Farm During 2004 D3 Self-fertility in beets: Obligate or facultative? D5 PROJECT 742 Sugar beet seedling disease resistance caused by Rhizoctonia D7 Phenotypic and Genotypic Analyses of Root Sucrose Accumulation During Juvenile to Adult Development Phase Change in Sugar Beet D11 Table 1: 04BB01 Agronomic performance D35 CONTENTS PAGE Table 2: Germination and emergence data for entries in Test 02BB01 D36 Table 3: 04BB01 Analytical methods comparison D37 Table 4: 00BB02 Evaluation Plots D38 Table 5: 04BB05 Recombinant Inbreds 1 D39 Table 6: Stand counts of entries tested in Test 04BB03 (Disease Nursery) D42 SECTION E - BELTSVILLE, MARYLAND Contents El PROJECT 811 Improvement of Root Maggot and Disease Resistance in Sugarbeet E2 PROJECT 831 Expression of the CFP Gene from Cercospora in Sugar Beet E7 SECTION F - BUSHLAND, TEXAS Contents FI PROJECT 508 Mutation and Genetic Variability among Isolates of BNYVV F2 SECTION G - FORT COLLINS, COLORADO Contents G1 PROJECT 840 New Strategies for Modifying Sucrose Distribution in Sugarbeet G2