studies on root-knot nematode, meloidogyne spp. on cucurbitaceous vegetables

110 Pages·2016·16.56 MB·English

STUDIES ON ROOT-KNOT NEMATODE, MELOIDOGYNE
SPP. ON CUCURBITACEOUS VEGETABLES

By

D.SAVITRI, B.Sc.(Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF
MASTER OF SCIENCE
IN THE FACULTY OF AGRICULTURAL SCIENCES
(ENTOMOLOGY)

DEPARTMENT OF ENTOMOLOGY
SRI VENKATESWARA AGRICULTURAL COLLEGE, TIRUPATI
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD – 500 030

SEPTEMBER, 2006
CERTIFICATE

Ms.D.SAVITRI has satisfactorily prosecuted the course of research
and that the thesis entitled “STUDIES ON ROOT-KNOT NEMATODE,
MELOIDOGYNE SPP. ON CUCURBITACEOUS VEGETABLES”
submitted is the result of original research work and is of sufficiently high
standard to warrant its presentation to the examination. I also certify that
the thesis or part thereof has not been previously submitted by her for a
degree of any University.

Date : 28.12.2006 Dr.T.UMA MAHESWARI
(Major Advisor)
CERTIFICATE

This is to certify that the thesis entitled “STUDIES ON ROOT-
KNOT NEMATODE, MELOIDOGYNE SPP. ON CUCURBITACEOUS
VEGETABLES” submitted in partial fulfillment of the requirements for the
degree of MASTER OF SCIENCE IN AGRICULTURE of the Acharya
N.G.Ranga Agricultural University, Hyderabad, is a record of the bonafide
research work carried out by Ms.D.SAVITRI under our guidance and
supervision. The subject of the thesis has been approved by the Student’s
Advisory Committee.

No part of the thesis has been submitted for any other degree or
diploma. The published part has been fully acknowledged. All assistance
and help received during the course of investigations have been duly
acknowledged by the author of the thesis.

Dr.T.UMA MAHESWARI
Chairman of the Advisory Committee

Thesis approved by the Student’s Advisory Committee

Chairman : Dr.T.UMA MAHESWARI _____________________
Associate Professor
Department of Entomology
College of Agriculture
Rajendranagar
Hyderabad – 500 030.

Member : Dr.S.SRINIVASAN _____________________
Professor & Head
Dept. of Entomology
S.V.Agricultural College
Tirupati - 517 502.

Member : Dr.M.REDDI KUMAR _____________________
Assistant Professor
Dept.of Plant Pathology
S.V.Agricultural College
Tirupati - 517 502.
DECLARATION

I, Ms.D.SAVITRI hereby declare that the thesis entitled “STUDIES
ON ROOT-KNOT NEMATODE, MELOIDOGYNE SPP. ON
CUCURBITACEOUS VEGETABLES” submitted to Acharya N.G Ranga
Agricultural University, Hyderabad for the degree of MASTER OF SCIENCE
IN AGRICULTURE majoring in ENTOMOLOGY is the result of original
research work done by me. I also declare that the materials contained in
this thesis have not been published earlier.

Date : 28.12.2006 (D.SAVITRI)
CONTENTS

Chapter Page
Title
No. No.
I INTRODUCTION
II REVIEW OF LITERATURE
III MATERIALS AND METHODS
IV RESULTS
V DISCUSSION
VI SUMMARY
LITERATURE CITED
LIST OF TABLES

Table Page
Title
No. No.
Occurrence of root-knot nematode on Gherkin in
1.
Chittoor district
Incidence of root-knot nematode in Gherkin fields
2.
surveyed in Chittoor district
Severity of root-knot nematode in Gherkin fields
3.
surveyed in Chittoor district
Pathogenicity of Meloidogyne incognita to Gherkin
4.
and its effect on plant growth characters
Effect of different inoculum levels of Meloidogyne
5. incognita on Gherkin root-knot damage and
nematode multiplication
Pathogenicity of Meloidogyne incognita to Ash gourd
6.
and its effect on plant growth characters
Effect of different inoculum levels of Meloidogyne
7. incognita on Ash gourd root-knot damage and
nematode multiplication
Screening of Gherkin cultivars for resistance to
8.
Meloidogyne incognita
Reaction of different cultivars of Gherkin to root-
9.
knot nematode, Meloidogyne incognita infection
Screening of Ash gourd cultivars for resistance to
10.
Meloidogyne incognita
Reaction of different cultivars of Ash gourd to root-
11.
knot nematode, Meloidogyne incognita infection
LIST OF ILLUSTRATIONS

Fig. Page
Title
No. No.
Incidence of root-knot nematode in Gherkin fields in
1.
Chittoor district
Effect of different inoculum levels of Meloidogyne
2.
incognita on number of fruits in Gherkin
Effect of different inoculum levels of Meloidogyne
3. incognita on number of galls and egg masses on
Gherkin
Effect of different inoculum levels of Meloidogyne
4. incognita on length and fresh weight of the shoot on
Ash gourd
Effect of different inoculum levels of Meloidogyne
5. incognita on number of galls and egg masses on Ash
gourd
Reaction of root-knot nematode, Meloidogyne
6.
incognita to Gherkin cultivars
Reaction of root-knot nematode, Meloidogyne
7.
incognita to Ash gourd cultivars
LIST OF PLATES

Plate Page
Title
No. No.
1a. Gherkin crop
Root system of Gherkin infected with root-knot
1b.
nematode, Meloidogyne incognita
Female of the root-knot nematode, Meloidogyne
2a.
incognita
Perineal pattern of the root-knot nematode,
2b.
Meloidogyne incognita
Pathogenicity of Meloidogyne incognita on plant
3a.
growth characters of Gherkin
Gherkin roots inoculated with various levels of
3b.
root-knot nematode, Meloidogyne incognita
Pathogenicity of Meloidogyne incognita on plant
4a.
growth characters of Ash gourd
Ash gourd roots inoculated with various levels of
4b.
root-knot nematode, Meloidogyne incognita
Screening of Gherkin cultivars against Meloidogyne
5a.
incognita
Gall formation by root-knot nematode, Meloidogyne
5b.
incognita in different cultivars of Gherkin
Screening of Ash gourd cultivars against
6a.
Meloidogyne incognita
Gall formation by root-knot nematode, Meloidogyne
6b.
incognita in different cultivars of Ash gourd
Acknowledgements

It is by the greatness of my parents, Smt.D.Lakshmamma and
Sri D.Subbarayudu, I was able to bring to light this humble piece of
work for which I am eternally indebted.
I am indeed indebted to LORD SIVA for His grace and fruitful
blessings on me.
I deem it a rare privilege to work under the guidance of esteemed
Chairman of the Advisory Committee, Dr.T.UMA MAHESWARI, Associate
Professor, Department of Entomology, College of Agriculture, R’nagar,
Hyderabad. Her meticulous guidance, constant encouragement, patient
hearing, seasoned constructive criticism, whole hearted pain taking
cooperation and sincerity towards duties instilled in me the spirit of
confidence to complete this task successfully for which I am grateful to
the rest of my life.
I humbly express my sincere thanks to Dr.S.SRINIVASAN,
Professor and Head, Department of Entomology, S.V.Agricultural College,
Tirupati for providing necessary facilities and kind technical help
extended to complete my research work.
I wish to place on record my sincere and heartfelt regards to
Dr.M.REDDI KUMAR, Assistant Professor, Department of Plant
Pathology, S.V.Agricultural College, Tirupati for his inspiring and
affectionate guidance and constant encouragement during my study.
I am extremely thankful to Dr.N.VENUGOPAL RAO, Professor
(Retd.), S.V. Agricultural College, Tirupati for his perspicacious comments,
valuable suggestions, which are of estimable in value in preparation of
the thesis manuscript. I owe him a huge debt of gratitude for ever for all
that I got from him.
I sincerely acknowledge the help rendered by the teaching staff of
Department of Entomology, Dr.P.Rajendra Prasad, Associate Professor,
Dr.V.Govardhana Naidu, Professor, Smt.P.Savitri, Assistant Professor
and Dr.K.Manjula, Assistant Professor, S.V.Agricultural College,
Tirupati.
I avail the opportunity with a profound sense of gratitude and
pleasure to express my heart bound sincere thanks to Sri Narama
Naidu and Smt.Madhavi, Department of Horticulture, S.V.Agricultural
College, Tirupati for their generous help, timely suggestions and able
guidance during the study.
Diction is not enough to express my unboundful gratitude to my
loving brother Subbaiah, sister-in-law Renuka, sisters Ramanamma,
Sunitha, brother-in-law Aswarthu, uncle Krishnaiah for their
uncountable moral support throughout my educational career. They have
been a fountain of inspiration throughout my life without whose
inexhaustible inspiration, everlasting love and moral support I would not
have been what I am today.
It’s but incomplete if I don’t pen here the pleasure showers of my
life Vani who have been a fountain of inspiration, giving a hand where
it’s needed, for her ever helping nature and pleasant company during my
study.