STUDIES ON GENETIC DIVERSITY ASSESSMENT OF SORGHUM

142 Pages·2016·3.39 MB·English

STUDIES ON GENETIC DIVERSITY
ASSESSMENT OF SORGHUM
(Sorghum bicolor L.Moench)
GENOTYPES AND HYBRID PURITY
TEST EMPLOYING SSR MARKERS

SUDARSHAN PATIL K.
B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE
(GENETICS AND PLANT BREEDING)

2011
STUDIES ON GENETIC DIVERSITY
ASSESSMENT OF SORGHUM (Sorghum
bicolor L.Moench) GENOTYPES AND
HYBRID PURITY TEST EMPLOYING SSR

MARKERS

By

SUDARSHAN PATIL K.
B.Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE
(GENETICS AND PLANT BREEDING)

DEPARTMENT OF GENETICS AND PLANT BREEDING
COLLEGE OF AGRICULTURE,
ACHARYA N.G.RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD-500 030, ANDHRA PRADESH
INDIA
JUNE, 2011
CERTIFICATE

Mr. SUDARSHAN PATIL K. has satisfactorily prosecuted the course of
research and that the thesis entitled “STUDIES ON GENETIC DIVERSITY
ASSESSMENT OF SORGHUM (Sorghum bicolor L.Moench) GENOTYPES
AND HYBRID PURITY TEST EMPLOYING SSR MARKERS” submitted, is
the result of original research work and is of sufficiently high standard to warrant
its presentation to the examination. I also certify that the thesis or part thereof has
not been previously submitted by his for a degree of any University.

Date : ( Dr. J.SURESH )
Place : Hyderabad
Chairperson
CERTIFICATE

This is to certify that the thesis entitled “STUDIES ON GENETIC
DIVERSITY ASSESSMENT OF SORGHUM (Sorghum bicolor L. Moench)
GENOTYPES AND HYBRID PURITY TEST EMPLOYING SSR
MARKERS” submitted in partial fulfillment of the requirements for the degree of
“MASTER OF SCIENCE IN AGRICUTLURE” of the Acharya N. G. Ranga
Agricultural University, Hyderabad, is a record of the bonafide research work
carried out by Mr. SUDARSHAN PATIL K., under my guidance and
supervision. The subject of the thesis has been approved by the Student's Advisory
Committee.

No part of the thesis has been submitted for any other degree or diploma. The
published part has been fully acknowledged. All assistance and help received
during the course of the investigation have been duly acknowledged by the author
of the thesis.

Thesis approved by the Student's Advisory Committee.

Chairperson : Dr. J.SURESH _____________________
Assistant Professor
College of Agriculture
Rajendranagar, Hyderabad- 30.

Co-Chairman: Dr. R. MADHUSUDHANA _____________________
Senior Scientist
Genetics and Plant breeding,
Directorate of Sorghum Research
Rajendranagar, Hyderabad-30.

Member : Dr. M.GANESH _____________________
Dean of Student Affairs,
Acharya N.G.Ranga Agricultural University,
Rajendranagar, Hyderabad-500 030.

Date of final viva-voce:
DECLARATION

I, Mr. SUDARSHAN PATIL K., hereby declare that the thesis entitled
“STUDIES ON GENETIC DIVERSITY ASSESSMENT OF SORGHUM
(Sorghum bicolor L.Moench) GENOTYPES AND HYBRID PURITY TEST
EMPLOYING SSR MARKERS” submitted to Acharya N. G. Ranga
Agricultural University for the Degree of MASTER OF SCIENCE IN
AGRICUTLURE is a result of original research work done by me. It is further
declared that the thesis or any part there of has not been published earlier in any
manner.

Date : (SUDARSHAN PATIL K.)
Place : Hyderabad I.D.No. RAM/2009-48
LIST OF CONTENTS

CHAPTER NO. TITLE PAGE NO.

I INTRODUCTION

II REVIEW OF LITERATURE

III MATERIAL AND METHODS

IV RESULTS AND DISCUSSION

V SUMMARY AND CONCLUSIONS

LITERATURE CITED

ANNEXURE
ACKNOWLEDGEMENTS
It is my heart’s turn to express my deepest sense of gratitude to all of those who directly
and indirectly helped me in this endeavour.
At the very outset, I fell inadequacy of words to express my profound indebtness and
deep sense of gratitude to my esteemed Chairman of Advisory Committee, Dr. J. Suresh,
Assistant Professor, college of Agriculture, Rajendranagar, Hyderabad for his scholastic
guidance, unceasing interest, constructive criticism, technical guidance, scientific view,
cherishabe conselling, warmer affection, caring nature, moral support and also his help in
bringing out this thesis. It was really a great pleasure and privilege for me to be associated
with him during my M.Sc. Degree programme.
I feel a great pleasure in expressing my whole hearted sense of gratitude to the Co-
Chairman of the Advisory Committee, Dr. R. Madhusudhana, Senior Scientist,
Directorate of Sorghum Research, Rajendranagar, Hyderabad for designing this beguiling
piece of work for my thesis, sincere technical guidance, learned counsel, unstinted attention
during the entire period of study. His keen interest, patience hearing, indomitable quest for
science and constructive criticism have installed in me the spirit of confidence to successfully
complete the task.
I deem it my privilege in expressing my fidelity to Member of Advisory Committee, Dr.
M. Ganesh, Director of Student Affairs, college of Agriculture, Rajendranagar, Hyderabad
for his constant encouraging, constructive suggestion, sustained interest for his constant
encouragement, constructive suggestions, fruitful advice and all necessary support during the
execution of thesis work.
I express my sincere gratitude to Dr. J.V. Pail, Project Director, Directorate of Rice
Research,, Rajendranagar, Hyderabad for providing the state-of-the art facilities and all
necessary support during the execution of this thesis work.
I feel great to express my heartfelt thanks to Dr.Rajendrakumar, Dr.
Hariprasanna, and Dr. Umakanth Senior Scientist, Crop Improvement Section,
Directorate of Sorghum Research, Rajendranagar, Hyderabad for their caring nature,
friendly co-operation and timely help during my thesis work.
I feel a great privilege in placing on record my profound thanks to Dr.
M.V.Brahmeshwara Rao, Professor and Head, for his moral support and timely help in
successful completion of my course. It’s an immense pleasure to express great, heartfelt respect to
my beloved Teachers Dr. Dayakar Reddy, Dr. Radhakrishna , Dr. Sudheer Kumar, Dr.
Bharathi, Dr. Ch. Durgarani, Dr. Radhika, Dr. Sujatha, Dr. Farzana jabeen, Mr.
Narendranath, Mr. Murali Krishna, Dr. Sumathi and Dr. Anuradha who have guided
me, all the way to reach up to this level.
I owe a lot to my parents, brother and friends, without whose affection, support and
sacrifice, this study would hardly have been accomplished.
My diction is poor to translate into words the sense of gratitude, heartfelt respect and
affection to my father Sri. Bharamana Gowda K, mother Smt. Basamma K, my
brothers Shambhu, Pramod, Chandru, sister-in-law Mrs.Gowri and my relatives
for their blessing, moral support, boundless love and building unshakable confidence in me
which motivated as a force to fulfill this long cherished ambition.
I feel to express my heartfelt, sincere thanks to Jaikishan, Bhaskar, Uttam,
Praveen, Madhu, Santosh, Chandrashekhar, Venkataramana and Shenbaga
Valli for their immense help, friendly co-operation and constant support during my work at
MAS lab., DSR, which will be remembered by me everlastingly.
It is rather difficult to express in words my deep sense of boundless affection, I would
heartly acknowledge the constant encouragement, assistance and constructive suggestions
given by seniors Nagesh Patne, Vemana Iraddi, Mallikarjun, Sripathi,
Panduranga, Rafi, Rangaswamy, Shailesh, Prashanth and Anand. I fondly thank
my juniors Shivu, Sooganna, Kailash, Deepak, Sudharshan and Sangamesh and all
others for all their help.
It is time to surface out my over whelming sense of affection to my friends Teju,
Umesh, Deepak, Devendra, Vithoba, Siddu, Karthik and Harish for whole hearted
co-operation to materialize this work.
I use this opportunity to sincerely thank my dearest classmates Rajendar, Sadaiaha,
Kotilingam, Gonya Naik, Sravani, Nivedita, Sandya and Khin Lay Kyu for their
lovely friendship, help and care and for making the two year study very much enjoyable and
memorable.
I am grateful to ICAR for the financial support in the form of ICAR-JRF during my
study period and the authorities of ANGRAU for having provided the opportunity to study
in the university.
Above all, my whole hearted prostrations to the Almighty for sprinkling his blessings
upon me.

Date: (SUDARSHAN PATIL K.)
LIST OF TABLES
Table No. Title Page No.
List of forty six sorghum genotypes used for
1
diversity analysis
List of three F1 sorghum hybrids used for hybrid
2
purity test
List of markers with their sequence employed in
3
molecular diversity analysis and hybrid purity test
of sorghum
Mean performance of forty four genotypes with
4
respect of twelve characters in sorghum
Analysis of variance for twelve characters in
5
sorghum genotypes
Estimation of variability, heritability and genetic
6
advance as per cent of mean for seven characters
in forty four genotypes of sorghum
Clustering of forty four genotypes based on
7
Ward’s Euclidean² analysis
Average intra and inter-cluster D² and D values
8
for thirteen clusters in forty four genotypes of
sorghum
Mean values of clusters for twelve characters in
9
forty four sorghum genotypes
Per cent contribution of characters towards
10
diversity in sorghum genotypes
List of polymorphic and monomorphic SSR
11
markers
Allelic frequency and polymorphism information
12
content values of forty two SSR markers