Structure and Function Relationships in Biochemical Systems

373 Pages·1982·13.75 MB·English

by  Noris Siliprandi (auth.), Francesco Bossa, Emilia Chiancone, Alessandro Finazzi-Agrò, Roberto Strom (eds.)

STRUCTURE
AND FUNCTION
RELATIONSHIPS IN
BIOCHEMICAL SYSTEMS
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University ofN ew York at Buffalo
NICHOLAS R. DI LUZIO, Tulane University School ofM edicine
EPHRAIM KATCHALSKI-KATZIR, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, Rockland Research Institute
RODOLFO PAOLETTI, University of Milan
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STRUCTURE AND FUNCTION RELATIONSHIPS IN BIOCHEMICAL SYSTEMS
Edited by Francesco Bossa, Emilia Chiancone, Alessandro Finazzi-Agro,
and Roberto Strom
STRUCTURE
AND FUNCTION
RELATIONSHIPS IN
BIOCHEMICAL SYSTEMS
Edited by
Francesco Bossa
University of Rome
Rome, Italy
Emilia Chiancone
CNR Center of Molecular Biology
Rome, Italy
and
Alessandro Finazzi -Agro
Roberto Strom
and
University of Rome
Rome, Italy
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
Symposium on Structure-Function Relationships in Biochemical Systems (1981: Acca
demia nazionale dei Lincei)
Structure and function relationships in biochemical systems.
(Advances in experimental medicine and biology; v. 148)
Includes bibliographical references and index.
1. Enzymes-Congresses. 2. Structure-activity relationship (Pharrnacology)-Con
gresses. 3. Biochemorphology-Congresses. 4. Hemoglobin-Congresses. 5. Sulphur
Metabolism-Congresses. I. Bossa, Francesco. II. Chiancone, Emilia. III. Title. IV.
Series. [DNLM: 1. Structure-activity relationship - Congresses. WI AD559 v. 148/ QU
34 S927 1981)
QP60I.S94931981 574.19'25 82-9139
AACR2
ISBN-13:978-1-4615-9283-9 e-ISBN-13:978-1-4615-9281-5
DOI: I 0.1007/978-1-4615-9281-5
Proceedings of a symposium on Structure-Function Relationships in
Biochemical Systems, celebrating the 75th birthday of Alessandro
Rossi Fanelli, held September 28-30, 1981, at the Accademia
Nazionale dei Lincei, in Rome, Italy
© 1982 Plenum Press, New York
Softcover reprint of the hardcover 1st edition 1982
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
PREFACE
Studies on the elucidation of structure-function relationships
in biochemistry and molecular biology started a few decades ago and
represent an area of continuing interest. The celebration of the
75th birthday of Alessandro Rossi Fanelli, who has made signifi-
cant contributions in this field, prompted the organization of a
Symposium on 'Structure-Function Relationships in Biochemical Systems'
with the aim of bringing together Rossi Fanelli's students and many
of the scientists who have been connected with the Institute of
Biological Chemistry in Rome. The Symposium was held in Rome on
September 28-30, 1981 at the Accademia Nazionale dei Lincei.
This volume contains the lectures presented at the Symposium
as well as articles covering the main themes of research conducted
in Rossi-Fanelli's Institute. The material is divided into five
sections: Hemoglobin, Myoglobin and Other Respiratory Proteins;
Mechanism of Action of Metal-Containing Enzym~s; Bioenergetics, Mem
brane Structure and Multienzyme Complexes; Cofactor-Dependent En
zymes; Sulfur Metabolism.
We gratefully the generous financial support of
acknow~edge
the Italian National Research Council and of the University of Rome,
which rendered the Symposium possible. We wish to thank all the con
tributors and Maurizio Gattoni, Paolo Gerosa and Mario Sanchioni
for their help in the preparation of the illustrative material. Fi
nally we wish to express our gratitude to Professor Rossi Fanelli,
the unfailing animator of our Institute; in his honour we happily
undertook the task of editing this volume.
Francesco Bossa
Emilia Chiancone
Alessandro Finazzi Agro
Roberto Strom
v
CONTENTS
Professor Rossi Fanelli: A Profile 1
N. Siliprandi
A Statistical Analysis of the Publications and
Collaborations of Alessandro Rossi Fanelli 7
W.E. Blumberg
HEMOGLOBIN, MYOGLOBIN, AND OTHER RESPIRATORY PROTEINS
Linkage Graphs 23
J. Wyman
Nature of the Iron-Oxygen Bond and Control of
Oxygen Affinity of the Haem by the Structure
of the Globin in Haemoglobin . • • . • • . • 31
M.F. Perutz
The Function of High Hemoglobin in Large Fish . • • • . . 49
Q.H. Gibson and F.G. Carey
Invertebrate Hemoglobins: The Dimeric Hemoglobin from
the Mollusc Scapharca inaequivalvis . . . . . • 67
E. Antonini, E. Chiancone, and F. Ascoli
Effects of Heavy Metals on the Respiratory Proteins
of Marine Organisms in Relation to Enviromental
Pollution . . • . • . . . • • . • . . • • . 75
J. Bonaventura, C. Bonaventura, and M. Brouwer
MECHANISM OF ACTION OF METAL-CONTAINING ENZYMES
Intermediates in the Reduction of Dioxygen by Laccase
and Cytochrome c Oxidase ..•.•.••.. 87
B.G. Malmstr5m
vii
viii CONTENTS
Structure and Function of the Redox Site of
Cytochrome Oxidase 95
B. Chance, L. Powers, and Y. Ching
Kinetic Studies of Cytochrome-a-Oxidase:
Significance of Different Functional
States of the Enzyme . • • • • • 111
M. Brunori, A. Colosimo, and M.T. Wilson
Non-Heme Components of Electron Transfer Systems:
Interactions and Conformational Effects •• 123
H. Beinert
Structure and Function of Amine Oxidases 141
B. Mondov~ and A. Finazzi Agrb
The Mechanism of Action of Cu,Zn Superoxide Dismutase • . 155
G. Rotilio, L. Calabrese, A. Rigo, and
E.M. Fielden
BIOENERGETICS, MEMBRANE STRUCTURE, AND MULTIENZYME COMPLEXES
Proton and Electric Charge Translocation in
Mitochondrial Energy Transduction 171
A.L. Lehninger
The Molecular Slipping in the Redox-Driven H+ Pumps • • • 187
G.F. Azzone, D. Pietrobon, and D. Walz
Structural Aspects of the Binding of Melittin to
Phospholipid Bilayers, as a Model for Protein-
Lipid Interactions in Membranes • • . . . . • . 195
R. Strom, F. Podo, C. Crifo, and G. Zaccai
Acetylcholine Receptors from Electroplax Membranes:
in vitro and in situ Properties . . • . • • • 209
M. Martinez-Carrion, J.M. Gonzalez-Ros,
M. Llanillo, and A. Paraschos
Interaction of Fusicoccin with Plant Cell Plasma
Membranes . . • . • . . • . • . • • . . . • 223
A. Ballio
Structure-Function Relationships in Pyruvate and
a-Ketoglutarate Dehydrogenase Complexes . • 231
L.J. Reed and R.M. Oliver
CONTENTS ix
Facilitation of Enzyme Catalysis by Formation of
Multienzyme Complexes • . • • •• •••. 243
C. Salerno and P. Fasella
COFACTOR-DEPENDENT ENZYMES
Some Comparative Aspects of Pyridoxal Phosphate
and Pyruvoyl-Dependent Amino Acid
Decarboxylases • . . • . • • • • • • • . 257
E.E. Snell
Structural Studies of Aspartate Aminotransferase
Isozymes .• . . • . • • . • • 273
D. Barra, F. Bossa, and S. Doonan
Recent Advances in the Study of Coenzyme Binding
to Aspartate Apoaminotransferases . . . • • 283
C. Turano, F. Riva, and A. Giartosio
Flavoproteins: Correlation of Structure and Function 295
V. Massey
Role of Flavin and Iron Sulfur Centers in the
Transition of Succinate Dehydrogenase from
the Activated to the Non-Activated Form . • 309
F. Bonomi, S. Pagani, and P. Cerletti
SULFUR METABOLISM
Biochemical Function of Persulfides . • . 327
J .L. Wood
Biological Utilization of Some Selenium- and
Sulfur-Containing Amino Acids . . . • • 343
C. De Marco and M. Di Girolamo
The Oxidation of Sulfur-Containing Amino Acids
by L-Amino Acid Oxidases . • • • . . . • 359
D. Cavallini, G. Ricci, G. Federici, M. Costa,
B. Pensa, R.M. Matarese, and M. Achilli
Contributors 375
Subj ect Index 381
ALESSANDRO ROSSI FANELLI
PROFESSOR ROSSI FANELLI: A PROFILE
Noris Si1iprandi
Institute of Biological Chemistry, University of Padua
35100 Padua, Italy
This symposium has been organized to celebrate the 75th birth
day of Professor Rossi Fanelli and the formal conclusion of his long
and incomparable academic life.
It is my great privilege, bestowed on me solely because of se
niority, to depict the personality which brought about the birth and
affirmation of his School.
Milestones of Rossi Fanelli's life were Naples, his birthplace,
Pavia, the seat of his first Chair, and Rome, his permanent abode.
The university career of Professor Rossi Fanelli at the Institute
of Biological Chemistry in the University of Naples began when he
took his degree in Medicine in 1929. He worked in the laboratory
directed by Professor Gaetano Quag1iarie110 until 1942, when he was
appointed to the Chair of Biochemistry in the Faculty of Science at
Pavia University. The scientific activity of the Neapolitan period,
which was developing while Italian biochemistry was taking its first
steps, already outlines the experimental talent and the scientific
originality of Rossi Fanelli. This first appeared in the perfectio
ning of precise microanalytical procedures for the determination of
various ions and of some amino acids. We today, who are used to such
sophisticated machinery for coping with scientific problems without
worrying too much about analytical methodology, cannot easily un
derstand what it was to have to transfer to biological materials,
and on a micro-scale, the classic methods of analytical chemistry:
the difficulties, doubts, failures, and cost in energy and time.
The natural leaning of Rossi Fanelli to accurate analytical expe
riments led him to achieve important results in that period. I re
member with admiration his great ability - manual too - in setting
up various rather complicated apparatus with "bits and pieces"