Stem Cells in Regenerative Medicine – Methods and Protocols; Volume 482 of Methods in Molecular Biology - Humana Press

440 Pages·2016·13.72 MB·English

by  Audet J.

Stem Cells in Regenerative Medicine
M E T H O D S I N M O L E C U L A R B I O L O G Y TM
John M. Walker, SERIES EDITOR
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M M B TM
E T H O D S I N O L E C U L A R I O L O G Y
Stem Cells in Regenerative Medicine
Edited by
Julie Audet and William L. Stanford
University of Toronto, Toronto, ON, Canada
Editors
JulieAudet WilliamL.Stanford
UniversityofToronto,Toronto,ON, UniversityofToronto,Toronto,ON,
Canada Canada
[email protected] [email protected]
SeriesEditor
JohnM.Walker
UniversityofHertfordshire
Hatfield,Herts.
UK
ISSN:1064-3745 e-ISSN:1940-6029
ISBN:978-1-58829-797-6 e-ISBN:978-1-59745-060-7
DOI10.1007/978-1-59745-060-7
LibraryofCongressControlNumber:2008939410
#HumanaPress,apartofSpringerScienceþBusinessMedia,LLC2009
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Preface
Regenerative Medicine is devoted to replacing diseased cells, tissues, or organs, or
repairing tissues in vivo by augmenting natural or inducing latent regenerative pro-
cesses. Underlying these goals is the manipulation – both expansion and directed
differentiation–ofstemcells,whicharetheprimarysourceofdenovotissueregenera-
tion and maintenance of organ homeostasis. In this book, Stem Cells in Regenerative
Medicine, we aim to provide biomedical researchers, clinicians and biomedical engi-
neersanupdatedrepresentationofthelandscapeofstemcell-basedtherapiesinawide
spectrumoftissuesystemsandontogenicstages,startingfromtheisolationandculture
of stem cells to their actual use in vivo. In this first edition, we have attempted to
compileafoundationofprotocolswhichcanberefinedinsubsequentpublications.We
hope that this series of protocols will contribute to the definition of standardized
proceduresfor the manipulationof somatic and embryonic stem cells in research and
clinicalapplications.
WilliamL.Stanford,Ph.D.
JulieAudet,Ph.D.
v
Contents
Preface..................................................................v
Contributors............................................................. xi
ColorPlates ............................................................. xv
PART I: STEM CELL SOURCES AND MANIPULATION
1 DerivationandManipulationofMurineEmbryonicStemCells ..................3
AlexanderMeissner,SarahEminli,andRudolfJaenisch
2 PatterningMouseandHumanEmbryonicStemCellsUsing
Micro-contactPrinting ................................................21
RaheemPeerani,CelineBauwens,EugeniaKumacheva,
andPeterW.Zandstra
3 EfficientGeneKnockdownsinHumanEmbryonicStemCells
UsingLentiviral-BasedRNAi............................................35
AsminTulpuleandGeorgeQ.Daley
4 MeasurementofCell-PenetratingPeptide-MediatedTransduction
ofAdultHematopoieticStemCells .......................................43
AzizaP.ManceurandJulieAudet
5 StemCellSourcesforRegenerativeMedicine ...............................55
AliM.Riazi,SarahY.Kwon,andWilliamL.Stanford
PART II: REGENERATION OF THE BLOOD SYSTEM
6 InvestigatingtheInteractionsBetweenHaemopoieticStemCells
andTheirNiche:MethodsfortheAnalysisofStemCellHoming
andDistributionWithintheMarrowFollowingTransplantation ................93
BrendaWilliamsandSusanK.Nilsson
7 ExVivoMegakaryocyteExpansionandPlateletProduction
fromHumanCordBloodStemCells ....................................109
Vale´rieCortin,NicolasPineault,andAlainGarnier
8 ExVivoGenerationofHumanRedBloodCells:ANewAdvance
inStemCellEngineering..............................................127
LucDouayandMarie-CatherineGiarratana
PART III: REGENERATION OF THE NERVOUS SYSTEM
9 IsolationandManipulationofMammalianNeuralStemCellsInVitro...........143
ClaudioGiachino,OnurBasak,andVerdonTaylor
10 Isolation,Expansion,andDifferentiationofMouse
Skin-DerivedPrecursors ..............................................159
KarlJ.L.FernandesandFredaD.Miller
vii
viii Contents
11 XenotransplantationofEmbryonicStemCell-DerivedMotorNeurons
intotheDevelopingChickSpinalCord...................................171
HynekWichterle,MirzaPeljto,andStephaneNedelec
12 TransplantationofNeuralStem/ProgenitorCellsintoDeveloping
andAdultCNS .....................................................185
TongZheng,GregoryP.MarshallII,K.AmyChen,andEricD.Laywell
13 TransplantationofEmbryonicStemCell-DerivedDopaminergic
NeuronsinMPTP-TreatedMonkeys ....................................199
JunTakahashi,YasushiTakagi,andHidemotoSaiki
PART IV: REGENERATION OF THE EPIDERMIS
14 IsolationandCultureofEpithelialStemCells..............................215
JonathanA.NowakandElaineFuchs
15 RegenerationofSkinandCorneabyTissueEngineering .....................233
DanielleLarouche,ClaudiePaquet,JulieFradette,
PatrickCarrier,Franc¸oisA.Auger,andLucieGermain
PART V: REGENERATION OF THE MUSCULOSKELETAL SYSTEM
16 ProspectiveIsolationofMesenchymalStemCellsfromMouse
CompactBone......................................................259
BrentonJ.Short,NathalieBrouard,andPaulJ.Simmons
17 Isolation,Propagation,andCharacterizationofHumanUmbilicalCord
PerivascularCells(HUCPVCs) .........................................269
RahulSarugaser,JaneEnnis,WilliamL.Stanford,
andJohnE.Davies
18 BoneMarrow-DerivedMesenchymalStemCells:Isolation,Expansion,
Characterization,ViralTransduction,andProduction
ofConditionedMedium ..............................................281
MassimilianoGnecchiandLuisG.Melo
19 TemplateDNA-StrandCo-SegregationandAsymmetricCellDivision
inSkeletalMuscleStemCells...........................................295
VasilyShinin,BarbaraGayraud-Morel,andShahragimTajbakhsh
20 IsolationandGraftingofSingleMuscleFibres .............................319
CharlotteA.CollinsandPeterS.Zammit
PART VI: REGENERATION OF THE VASCULAR SYSTEM
21 DifferentiationandDynamicAnalysisofPrimitiveVessels
fromEmbryonicStemCells............................................333
GefeiZengandVictoriaL.Bautch
22 DerivationofContractileSmoothMuscleCellsfromEmbryonic
StemCells .........................................................345
SanjaySinha,MarkH.Hoofnagle,andGaryK.Owens
PART VII: REGENERATION OF THE PANCREAS AND LIVER
23 Islet-DerivedProgenitorsasaSourceofInVitroIsletRegeneration.............371
StephenHanleyandLawrenceRosenberg
Contents ix
24 IsolationandCharacterizationofHepaticStemCells,or‘‘OvalCells,’’
fromRatLivers .....................................................387
ThomasD.Shupe,AnnaC.Piscaglia,Seh-HoonOh,AntonioGasbarrini,
andBryonE.Petersen
25 ReprogrammingofLivertoPancreas ....................................407
ShifaanThowfeequ,Wan-ChunLi,JonathanM.W.Slack,
andDavidTosh
Index.................................................................419
Contributors
JULIEAUDET,PHD(cid:2) TerrenceDonnellyCentreforCellularandBiomolecularResearch,
Institute of Biomaterials and Biomedical Engineering, University of Toronto,
Toronto,Ontario,Canada
FRANC¸OISA.AUGER,MD (cid:2)ExperimentalOrganogenesisLaboratory/LOEXand
Department of Surgery and Ophthalmology, Laval University, Sainte-Foy, Quebec,
Canada
ONURBASAK(cid:2)DepartmentofMolecularEmbryology,MaxPlanckInstituteof
Immunobiology,Freiburg,Germany
VICTORIAL.BAUTCH,PHD(cid:2)DepartmentofBiology,CarolinaCardiovascularBiology
Center,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC,USA
CELINEBAUWENS,MSc(cid:2)TerrenceDonnellyCentreforCellularandBiomolecular
Research, Institute of Biomaterials and Biomedical Engineering, University of
Toronto,Toronto,Ontario,Canada
NATHALIEBROUARD,PHD(cid:2)PeterMacCallumCancerCentre,Victoria,Australia
PATRICKCARRIER,MSc(cid:2) ExperimentalOrganogenesisLaboratory/LOEXand
Department of Surgery and Ophthalmology, Laval University, Sainte-Foy, Quebec,
Canada
K.AMYCHEN (cid:2)DepartmentofNeuroscience,McKnightBrainInstitute,Universityof
Florida,Gainesville,FL,USA
CHARLOTTEA.COLLINS,PHD(cid:2) WellcomeTrustCentreforStemCellResearch,University
ofCambridge,Cambridge,UnitedKingdom
VALE´RIECORTIN,PHD(cid:2)De´partementdeRechercheetDe´veloppement,He´ma-Que´bec,
Que´becCity,Que´bec,Canada
GEORGEQ.DALEY,MD,PHD(cid:2)HarvardMedicalSchool,KarpFamilyResearchBuilding,
DivisionofHematology/Oncology,Children’sHospitalBoston,Boston,MA,USA
JOHNE.DAVIES,PHD,DSc(cid:2)InstituteofBiomaterialsandBiomedicalEngineering,
UniversityofToronto,Toronto,Ontario,Canada
LUCDOUAY,MD,PHD(cid:2) Unite´ derecherche´,Faculte´ deMe´decine,Universite´ Pierreet
Marie Curie, Paris, France, and Service d’He´matologie Biologique, Assistance
PubliqueHoˆpitauxdeParis,HoˆpitalArmandTrousseau,Paris,France
SARAHEMINLI,PHD(cid:2)CenterforRegenerativeMedicineandCancerCenter,
Massachusetts General Hospital, Harvard Medical School and Harvard Stem Cell
Institute,Boston,MA,USA
JANEENNIS,MASC (cid:2)InstituteofBiomaterialsandBiomedicalEngineering,Universityof
Toronto,Toronto,Ontario,Canada
KARLJ.L.FERNANDES,PHD(cid:2)De´partementdepathologieetbiologiecellulaire,Universite´
deMontre´al,Montre´al,Que´bec,Canada
xi