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Standard operational procedures in reproductive medicine : laboratory and clinical practice PDF

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Standard Operational Procedures in Reproductive Medicine Laboratory and Clinical Practice REPRODUCTIVE MEDICINE AND ASSISTED REPRODUCTIVE TECHNIQUES SERIES David Gardner University of Melbourne, Australia Zeev Shoham Kaplan Hospital, Rehovot, Israel Kay Elder, Jacques Cohen Human Preimplantation Embryo Selection, ISBN: 9780415399739 John D Aplin, Asgerally T Fazleabas, Stanley R Glasser, Linda C Giudice The Endometrium, Second Edition, ISBN: 9780415385831 Nick Macklon, Ian Greer, Eric Steegers Textbook of Periconceptional Medicine, ISBN: 9780415458924 Andrea Borini, Giovanni Coticchio Preservation of Human Oocytes, ISBN: 9780415476799 Steven R Bayer, Michael M Alper, Alan S Penzias The Boston IVF Handbook of Infertility, Third Edition, ISBN: 9781841848105 Ben Cohlen, Willem Ombelet Intra-Uterine Insemination: Evidence Based Guidelines for Daily Practice, ISBN: 9781841849881 Adam H. Balen Infertility in Practice, Fourth Edition, ISBN: 9781841848495 Nick Macklon IVF in the Medically Complicated Patient, Second Edition: A Guide to Management, ISBN: 9781482206692 Michael Tucker, Juergen Liebermann Vitrification in Assisted Reproduction, ISBN: 9780415408820 Ben J Cohlen, Evert J P van Santbrink, Joop S E Laven Ovulation Induction: Evidence Based Guidelines for Daily Practice, ISBN: 9781498704076 Botros Rizk, Markus Montag Standard Operational Procedures in Reproductive Medicine: Laboratory and Clinical Practice, ISBN: 9781498719216 Standard Operational Procedures in Reproductive Medicine Laboratory and Clinical Practice Edited by Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS Professor and Head of Reproductive Endocrinology and Infertility Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction University of South Alabama Mobile, Alabama, USA Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility Obstetrics and Gynecology King Abdulaziz University Jeddah, Kingdom of Saudi Arabia Distinguished Adjunct Scientific Director IVF Michigan Rochester Hills, Michigan, USA Markus Montag, PhD Professor and CEO ilabcomm GmbH Sankt Augustin, NRW, Germany CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2017 by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Printed on acid-free paper International Standard Book Number-13: 978-1-4987-1921-6 (Paperback) This book contains information obtained from authentic and highly regarded sources. While all reasonable efforts have been made to publish reliable data and information, neither the author[s] nor the publisher can accept any legal responsibil- ity or liability for any errors or omissions that may be made. The publishers wish to make clear that any views or opinions expressed in this book by individual editors, authors or contributors are personal to them and do not necessarily reflect the views/opinions of the publishers. The information or guidance contained in this book is intended for use by medical, scientific or health-care professionals and is provided strictly as a supplement to the medical or other professional’s own judgement, their knowledge of the patient’s medical history, relevant manufacturer’s instructions and the appropriate best practice guidelines. Because of the rapid advances in medical science, any information or advice on dosages, procedures or diagnoses should be independently verified. The reader is strongly urged to consult the relevant national drug formulary and the drug companies’ and device or material manufacturers’ printed instructions, and their websites, before administering or utilizing any of the drugs, devices or materials mentioned in this book. This book does not indicate whether a particular treatment is appropriate or suitable for a particular individual. Ultimately it is the sole responsibility of the medical profes- sional to make his or her own professional judgements, so as to advise and treat patients appropriately. The authors and publishers have also attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, micro- filming, and recording, or in any information storage or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please access www.copyright.com (http://www. copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750- 8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of users. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for iden- tification and explanation without intent to infringe. Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com To my father, Mitry Botros Rizk, 1916–2003, a great and most caring man who inspired me every day of my life and lived to do that. Contents About the Editors .....................................................................................................................................................................xi List of Contributors ................................................................................................................................................................xiii Section I Laboratory Procedures 1. The Documentation System .............................................................................................................................................2 Markus Montag and Lars Johansson 2. Culture Media Techniques ..............................................................................................................................................4 Lars Johansson 3. IVF Incubator Handling .................................................................................................................................................8 Jason E. Swain and Alex Lagunov 4. Preparation for Follicle Aspiration and Isolation of Cumulus-Oocyte-Complexes (COC) ....................................12 Markus Montag 5. Semen Collection and Sample Reception .....................................................................................................................14 Carin Huyser 6. Spermiogramm, Makler Chamber, Neubauer Chamber, CASA ..............................................................................18 Verena Nordhoff 7. Sperm Viability Testing .................................................................................................................................................20 Markus Montag 8. Sperm Preparation—Swim Up, Density Gradient, Migration Chamber, Zeta Method .........................................22 Thomas Ebner 9. Sperm Selection Using Hyaluronan Binding ...............................................................................................................24 Denny Sakkas 10. IVF—Insemination.........................................................................................................................................................26 Markus Montag 11. ICSI ..................................................................................................................................................................................28 Thomas Freour 12. Oocyte In Vitro Maturation—Isolation of COCs and Culture for Maturation .......................................................30 Mariabeatrice Dal Canto and Giovanni Coticchio 13. Artificial Oocyte Activation ..........................................................................................................................................32 Markus Montag 14. Cryopreservation—Sperm TESE and MESA .............................................................................................................34 Borut Kovačič 15. Vitrification—Oocytes, 2PN, Embryos, Blastocysts ...................................................................................................36 Ana Cobo and Aila Coello viii Contents 16. Slow Freezing: Principles, Oocytes, 2PN, Embryos, Blastocysts ..............................................................................38 Etienne Van den Abbeel 17. Oocyte Selection .............................................................................................................................................................40 Basak Balaban 18. Zygote Selection ..............................................................................................................................................................42 Martin Greuner 19. Selection and Grading of Embryos on Day 2/3 and Blastocysts on Day 5/6 .............................................................44 Thorir Hardarson and Julius Hreinsson 20. Time-Lapse Imaging ......................................................................................................................................................46 Cristina Hickman 21. Assisted Hatching ...........................................................................................................................................................50 Christian De Geyter and Maria De Geyter 22. Zona Manipulation at Various Embryo Stages: Mechanical/Acidic Medium .........................................................52 Marius Meintjes 23. Biopsy: Polar Body, Blastomere, Trophectoderm .......................................................................................................56 Hakan Yelke and Semra Kahraman 24. Sampling of Cells after Biopsy for aCGH ....................................................................................................................60 Barry Behr 25. Embryo Transfer—Standard and Ultrasound Guided ..............................................................................................62 Bettina Toth 26. Hygienic Standards in the IVF Laboratory ................................................................................................................64 Vera Baukloh 27. Quality Control of IVF Laboratory Supplies—Mouse Embryo Assay (MEA) ........................................................68 Dean E. Morbeck 28. Troubleshooting in the IVF Laboratory ......................................................................................................................70 David Mortimer and Sharon T. Mortimer 29. Microscopy ......................................................................................................................................................................72 Markus Montag 30. Fertility Preservation .....................................................................................................................................................74 Markus Montag and Jana Liebenthron Section II Clinical Procedures 31. Ultrasound Evaluation in Treatment Management ....................................................................................................78 Ippokratis Sarris and Geeta Nargund 32. Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies ...............................................................80 Magued Adel Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk 33. Infertility Ultrasound Evaluation: One-Stop Procedure ...........................................................................................82 Spiros A. Liatsikos and Geeta Nargund Contents ix 34. Office Hysteroscopy ........................................................................................................................................................86 Linda D. Bradley 35. Hysteroscopic Management of Uterine Septum ..........................................................................................................88 Omar Abuzeid and Mostafa Abuzeid 36. Hysteroscopic Lysis of Intrauterine Adhesions ...........................................................................................................92 Bolonduro Oluwamuyiwa and Mostafa Abuzeid 37. Hysteroscopic Management of Submucosal Fibroids .................................................................................................94 Salem Joseph and Mostafa Abuzeid 38. Saline Infusion Sonohysterography ..............................................................................................................................98 Alan Bolnick and Mostafa Abuzeid 39. General Principles of Laparoscopy ............................................................................................................................100 Rubin Raju and Mostafa Abuzeid 40. Microlaparoscopy .........................................................................................................................................................104 Oscar D. Almeida, Jr. 41. Laparoscopic Myomectomy and Mini-Laparotomy: An Alternative Approach for Myomectomy .....................106 Ahmed Abdelaziz and Mostafa Abuzeid 42. Laparoscopic Excision of a Large Endometrioma ....................................................................................................108 Rubin Raju and Mostafa Abuzeid 43. Laparoscopic Management of Subtle Fimbrial Pathology Secondary to Endometriosis .....................................110 Omar Abuzeid and Mostafa Abuzeid 44. Laparoscopic Management of Mature Cystic Teratoma..........................................................................................112 Rahima Sanya and Mostafa Abuzeid 45. Tubal Cannulation for Proximal Tubal Disease ........................................................................................................114 Wael Salem, Osama A.H. Abu Zinadah, Joshua Ekladios, and Botros Rizk 46. Robotic Assisted Laparoscopic Myomectomy ...........................................................................................................116 Hashem Lotfy, Arsany Bassily, Lauren Mann, and Botros Rizk 47. Robotic Assisted Tubal Anastomosis after Tubal Sterilization ...............................................................................118 Hashem Lotfy, Rola Turki, and Botros Rizk 48. Unexplained Infertility ................................................................................................................................................120 Hassan N. Sallam 49. Male Evaluation for Infertility ....................................................................................................................................122 Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal 50. Hysterosalpingography ................................................................................................................................................124 Islam Fahmi, Mostafa Abuzeid, and Shawky Z.A. Badawy 51. Intrauterine Insemination: Clinical Perspectives .....................................................................................................128 Willem Ombelet 52. GnRH Agonist to Trigger Final Follicular Maturation in IVF...............................................................................132 Nayana Talukdar and Robert F. Casper

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