Sphingosine-1-Phosphate: Methods and Protocols

185 Pages·2018·4.655 MB·English

by  Alice Pébay,Kursad Turksen (eds.)

Methods in
Molecular Biology 1697
Alice Pébay
Kursad Turksen
Editors
Sphingosine-
1-Phosphate
Methods and Protocols
Second Edition
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire,AL109AB,UK
Forfurther volumes:
http://www.springer.com/series/7651
Sphingosine-1-Phosphate
Methods and Protocols
Second Edition
Edited by
Alice Pébay
Centre for Eye Research Australia, The University of Melbourne, East Melbourne, VIC, Australia
Kursad Turksen
Ottawa Hospital Research Institute, Ottawa, ON, Canada
Editors
AlicePe´bay KursadTurksen
CentreforEyeResearchAustralia OttawaHospitalResearchInstitute
TheUniversityofMelbourne Ottawa,ON,Canada
EastMelbourne,VIC,Australia
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-4939-7412-2 ISBN978-1-4939-7413-9 (eBook)
https://doi.org/10.1007/978-1-4939-7413-9
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Preface
Since the first edition of this volume, the many new and important advances in this fast-
movingfieldpromptedustosolicitupdatesforprotocolsthatwethoughtwouldbevaluable
forbothexpertsandnovicesalike.
Wearethusgratefultoallofthecontributorswhotooktimetopresenttheirprotocols
in the useful format for which the Methods in Molecular Biology series is known. We thank
them for this and hope that this volume will be as valuable as the first edition was found
tobe.
We acknowledge the support and guidance of Dr. John Walker, Editor-in-Chief of the
Methods in Molecular Biology series, for giving us the opportunity to put this volume
together.PatrickMarton,ExecutiveEditoroftheSpringerProtocolsseries,providedcontin-
uoussupportandencouragementfromthestarttocompletionofthisproject-Thankyou.
David Casey provided tremendous help and hands-on support by helping us to elimi-
nateanymissingpartsanddetailsinchapters.Aspecialthankyouforthisoutstandingeffort
goestohim.
Melbourne,VIC,Australia AlicePe´bay
Ottawa,ON,Canada KursadTurksen
v
Contents
Preface ..................................................................... v
Contributors................................................................. ix
MeasuringSphingosine-1-Phosphate/ProteinInteractions
withtheKineticExclusionAssay .............................................. 1
JonathanK.FlemingandJonathanM.Wojciak
AnImprovedIsoform-SelectiveAssayforSphingosineKinase
1Activity................................................................... 9
MelissaR.Pitman,LorenaT.Davies,andStuartM.Pitson
MethodsforAnalyzingSphingosine-1-PhosphateSignaling
inHumanandMousePrimaryMastCells...................................... 21
AlenaP.Chumanevich,PiperA.Wedman,andCaroleA.Oskeritzian
MeasurementofLysophosphatidicAcidandSphingosine-1-Phosphate
byLiquidChromatography-CoupledElectrosprayIonizationTandem
MassSpectrometry .......................................................... 31
MariaP.Kraemer,SuchismitaHalder,SusanS.Smyth,
andAndrewJ.Morris
ImmunohistochemicalDetectionofSphingosine-1-Phosphate
andSphingosineKinase-1inHumanTissueSamplesandCellLines............... 43
GaryM.Reynolds,BarbaraVisentin,andRogerSabbadini
ACleanupMethodforMassSpectrometricAnalysisofSphingosine-
andCeramide-1-PhosphateinBloodandSolidTissueUsingaPhosphate
CaptureMolecule ........................................................... 57
Jun-ichiMorishige,RyouheiYamashita,TamotsuTanaka,
andKiyoshiSatouchi
ARapidFluorescenceAssayforMeasuringSphingosine-1-Phosphate
TransporterActivityinErythrocytes........................................... 73
NaokiKobayashiandTsuyoshiNishi
AnalysisofS1PReceptorExpressionbyUterineImmuneCells
UsingStandardizedMulti-parametricFlowCytometry........................... 83
JianhongZhang,AnnieBang,andStephenJ.Lye
S1PSynergizeswithWallShearStressandOtherAngiogenicFactors
toInduceEndothelialCellSproutingResponses ................................ 99
CamilleL.Duran,RolandKaunas,andKaylaJ.Bayless
InVitroMethodstoStudytheModulationofMigration
andInvasionbySphingosine-1-Phosphate...................................... 117
MelinaG.Castro,LudmilaE.Campos,YamilaI.Rodriguez,
andSergioE.Alvarez
MaintenanceofHumanEmbryonicStemCellsbySphingosine-1-Phosphate
andPlatelet-DerivedGrowthFactor........................................... 133
RaymondC.B.Wong,MartinF.Pera,andAlicePe´bay
vii
viii Contents
Sphingosine-1-Phosphate(S1P)SignalinginNeuralProgenitors.................. 141
PhillipCallihan,MohammedAlqinyah,andShelleyB.Hooks
CeramideandS1PSignalinginEmbryonicStemCell
Differentiation.............................................................. 153
GuanghuWang,StefkaD.Spassieva,andErhardBieberich
3DStackedConstruct:ANovelSubstituteforCornealTissue
Engineering ................................................................ 173
ShresthaPriyadarsini,SarahE.Nicholas,andDimitriosKaramichos
Index ...................................................................... 181
Contributors
MOHAMMEDALQINYAH (cid:1) DepartmentofPharmaceuticalandBiomedicalSciences,University
ofGeorgia,Athens,GA,USA
SERGIOE.ALVAREZ (cid:1) InstitutoMultidisciplinariodeInvestigacionesBiol(cid:1)ogicasSanLuis
(IMIBIO-SL)CONICETandUniversidadNacionaldeSanLuis,SanLuis,Argentina
ANNIEBANG (cid:1) FlowCytometryFacilities,Lunenfeld-TanenbaumResearchInstitute,Mount
SinaiHospital,Toronto,ON,Canada
KAYLAJ.BAYLESS (cid:1) DepartmentofMolecularandCellularMedicine,TexasA&MUniversity
HealthScienceCenter,CollegeStation,TX,USA
ERHARDBIEBERICH (cid:1) DepartmentofNeuroscienceandRegenerativeMedicine,Medical
CollegeofGeorgia,AugustaUniversity,Augusta,GA,USA
PHILLIPCALLIHAN (cid:1) DepartmentofPharmaceuticalandBiomedicalSciences,Universityof
Georgia,Athens,GA,USA
LUDMILAE.CAMPOS (cid:1) InstitutoMultidisciplinariodeInvestigacionesBiol(cid:1)ogicasSanLuis
(IMIBIO-SL)CONICETandUniversidadNacionaldeSanLuis,SanLuis,Argentina
MELINAG.CASTRO (cid:1) InstitutoMultidisciplinariodeInvestigacionesBiol(cid:1)ogicasSanLuis
(IMIBIO-SL)CONICETandUniversidadNacionaldeSanLuis,SanLuis,Argentina
ALENAP.CHUMANEVICH (cid:1) DepartmentofPathology,MicrobiologyandImmunology,
UniversityofSouthCarolinaSchoolofMedicine,Columbia,SC,USA
LORENAT.DAVIES (cid:1) MolecularSignallingLaboratory,CentreforCancerBiology,University
ofSouthAustraliaandSAPathology,Adelaide,SA,Australia
CAMILLEL.DURAN (cid:1) DepartmentofMolecularandCellularMedicine,TexasA&M
UniversityHealthScienceCenter,CollegeStation,TX,USA
JONATHANK.FLEMING (cid:1) LpathIncorporated,SanDiego,CA,USA
SUCHISMITAHALDER (cid:1) GillHeartandVascularInstitute,UniversityofKentuckyCollegeof
Medicine,LexingtonVeteransAffairsMedicalCenter,Lexington,KY,USA
SHELLEYB.HOOKS (cid:1) DepartmentofPharmaceuticalandBiomedicalSciences,Universityof
Georgia,Athens,GA,USA
DIMITRIOSKARAMICHOS (cid:1) DepartmentofOphthalmology/DeanMcGeeEyeInstitute,
UniversityofOklahomaHealthSciencesCenter,OklahomaCity,OK,USA
ROLAND KAUNAS (cid:1) DepartmentofBiomedicalEngineering,TexasA&MUniversity,
CollegeStation,TX,USA
SATOUCHIKIYOSHI (cid:1) DepartmentofAppliedBiologicalScience,FukuyamaUniversity,
Fukuyama,Japan
NAOKIKOBAYASHI (cid:1) DepartmentofBiochemistry,FacultyofPharmaceuticalSciences,
SetsunanUniversity,Hirakata,Osaka,Japan
MARIAP.KRAEMER (cid:1) GillHeartandVascularInstitute,UniversityofKentuckyCollegeof
Medicine,LexingtonVeteransAffairsMedicalCenter,Lexington,KY,USA
STEPHENJ.LYE (cid:1) ResearchCentreforWomen’sandInfants’Health,Lunenfeld-Tanenbaum
ResearchInstitute,MountSinaiHospital,Toronto,ON,Canada;DepartmentofObstetrics
andGynaecology,UniversityofToronto,Toronto,ON,Canada;DepartmentofPhysiology,
UniversityofToronto,Toronto,ON,Canada
JUN-ICHIMORISHIGE (cid:1) DepartmentofCellularandMolecularFunctionAnalysis,
KanazawaUniversityGraduateSchoolofMedicalScience,Kanazawa,Japan
ix
x Contributors
ANDREWJ.MORRIS (cid:1) GillHeartandVascularInstitute,UniversityofKentuckyCollegeof
Medicine,LexingtonVeteransAffairsMedicalCenter,Lexington,KY,USA
SARAH E.NICHOLAS (cid:1) DepartmentofOphthalmology/DeanMcGeeEyeInstitute,University
ofOklahomaHealthSciencesCenter,OklahomaCity,OK,USA
TSUYOSHINISHI (cid:1) DepartmentofBiomolecularScienceandRegulation,InstituteofScientific
andIndustrialResearch,OsakaUniversity,Ibaraki,Osaka,Japan;Facultyof
PharmaceuticalScience,OsakaUniversity,Suita,Osaka,Japan
CAROLEA.OSKERITZIAN (cid:1) DepartmentofPathology,MicrobiologyandImmunology,
UniversityofSouthCarolinaSchoolofMedicine,Columbia,SC,USA
ALICEPE´BAY (cid:1) CentreforEyeResearchAustralia,RoyalVictorianEyeandEarHospital,
TheUniversityofMelbourne,Melbourne,VIC,Australia;Ophthalmology,Department
ofSurgery,TheUniversityofMelbourne,Melbourne,VIC,Australia
MARTINF.PERA (cid:1) DepartmentofAnatomyandNeurosciences,FloreyNeuroscienceand
MentalHealthInstitute,WalterandElizaHallInstituteofMedicalResearch,The
UniversityofMelbourne,Melbourne,VIC,Australia
MELISSAR.PITMAN (cid:1) MolecularSignallingLaboratory,CentreforCancerBiology,
UniversityofSouthAustraliaandSAPathology,Adelaide,SA,Australia
STUARTM.PITSON (cid:1) MolecularSignallingLaboratory,CentreforCancerBiology,
UniversityofSouthAustraliaandSAPathology,Adelaide,SA,Australia
SHRESTHAPRIYADARSINI (cid:1) DepartmentofOphthalmology/DeanMcGeeEyeInstitute,
UniversityofOklahomaHealthSciencesCenter,OklahomaCity,OK,USA
GARYM.REYNOLDS (cid:1) CentreforLiverResearchandNIHRBiomedicalResearchUnit,
UniversityofBirminghamandQueenElizabethHospitalBirmingham,Birmingham,UK
YAMILA I.RODRIGUEZ (cid:1) InstitutoMultidisciplinariodeInvestigacionesBiol(cid:1)ogicasSanLuis
(IMIBIO-SL)CONICETandUniversidadNacionaldeSanLuis,SanLuis,Argentina
ROGERSABBADINI (cid:1) StanfordUniversitySchoolofMedicine,Stanford,CA,USA
SUSAN S.SMYTH (cid:1) GillHeartandVascularInstitute,UniversityofKentuckyCollege
ofMedicine,LexingtonVeteransAffairsMedicalCenter,Lexington,KY,USA
STEFKAD.SPASSIEVA (cid:1) DepartmentofMolecularandCellularMedicine,TexasA&M
MedicalHealthSciencesCenter,Bryan,TX,USA
TAMOTSUTANAKA (cid:1) InstituteofBiomedicalSciences,TokushimaUniversityGraduateSchool,
Tokushima,Japan
BARBARAVISENTIN (cid:1) LaJollaBiologicsInc,SanDiego,CA,USA
GUANGHUWANG (cid:1) DepartmentofNeuroscienceandRegenerativeMedicine,MedicalCollege
ofGeorgia,AugustaUniversity,Augusta,GA,USA
PIPERA.WEDMAN (cid:1) DepartmentofPathology,MicrobiologyandImmunology,University
ofSouthCarolinaSchoolofMedicine,Columbia,SC,USA
JONATHANM.WOJCIAK (cid:1) LpathIncorporated,SanDiego,CA,USA
RAYMOND C.B.WONG (cid:1) CentreforEyeResearchAustralia,RoyalVictorianEyeandEar
Hospital,TheUniversityofMelbourne,Melbourne,VIC,Australia;Ophthalmology,
DepartmentofSurgery,TheUniversityofMelbourne,Melbourne,VIC,Australia
RYOUHEIYAMASHITA (cid:1) InstituteofBiomedicalSciences,TokushimaUniversityGraduate
School,Tokushima,Japan
JIANHONGZHANG (cid:1) ResearchCentreforWomen’sandInfants’Health,Lunenfeld-
TanenbaumResearchInstitute,MountSinaiHospital,Toronto,ON,Canada
MethodsinMolecularBiology(2017)1697:1–8
DOI10.1007/7651_2017_5
©SpringerScience+BusinessMediaNewYork2017
Publishedonline:28March2017
Measuring Sphingosine-1-Phosphate/Protein Interactions
with the Kinetic Exclusion Assay
Jonathan K. Fleming and Jonathan M. Wojciak
Abstract
®
By directly detecting the ligand-free binding sites in a sample, the kinetic exclusion assay (KinExA )
providesacompellingalternativetoSPR-basedtechniquesfordeterminingequilibriumdissociationcon-
stants of protein-ligand interactions. It is especially useful for observing protein-lipid interactions, as
binding of native lipids occurs entirely in solution, and monoclonal antibodies can be used to directly
compete with a protein of interest for lipid binding. By measuring the antigen-free binding sites on the
antibodyandusingcompetitionaffinityanalysis,theK forthelipidbindingtheproteinandtheantibody
d
canbedeterminedsimultaneously.Herein,wedescribethislabel-freeapproachfordeterminingtheK for
d
S1P-bindingserumalbumin,whichchaperones~30%oftheS1Pinhumanplasma.
Keywords: Anti-lipidantibody,Bovineserumalbumin,Competitiveaffinityanalysis,Humanserum
albumin,Kineticexclusionassay,Physicalbiochemistry,Sphingosine-1-phosphate
1 Introduction
Theabilitytodetermineaccurate,reliableequilibriumdissociation
constants for proteins binding lysophospholipids, such as S1P, is
challengingduetoitslackofinherenttraceablecharacteristics(e.g.,
fluorescence or UV/Vis absorption). Modifying S1P either by
covalently attaching bulky tags or derivatization potentially alters
the solubility properties and/or natural mode of protein recogni-
tion [1]. In addition, binding studies that rely on physical separa-
tion of free and bound S1P species can be difficult because S1P
demonstrates poor solubility properties in aqueous media. The
aqueous media is, however, necessary to support the structure
and function of the protein. To overcome these issues, typically
S1P is delivered in complex with fatty acid-free bovine serum
albumin (FAF-BSA) [2]. However, the intrinsic affinity of FAF-
BSAforS1P(orotherlysophospholipids)mayconfoundthebind-
ing event being investigated and is seldom considered while inter-
pretingthedata.
The KinExA approach described here both overcomes the
challenges described above for studying protein-lipid interactions
and elucidates the effect of using carrier proteins to deliver
1