Edited by Roman Gr. Maev Advances in Acoustic Microscopy and High Resolution Imaging Related Titles Marage, J.-P., Mori, Y. Kutz, M. (ed.) Sonars and Underwater Mechanical Engineers’ Acoustics Handbook Materials and Mechanical Design Hardcover ISBN: 978-1-84821-189-6 2005 Hardcover Hodges, R. P. ISBN: 978-0-471-71985-4 Underwater Acoustics Oppelt, A. (ed.) Analysis, Design and Performance Imaging Systems for Medical of Sonar Diagnostics Hardcover Fundamentals, technical solutions and ISBN: 978-0-470-68875-5 applications for systems applying ionization radiation, nuclear magnetic Azhari, H. resonance and ultrasound Basics of Biomedical 2005 Ultrasound for Engineers Hardcover ISBN: 978-3-89578-226-8 Hardcover ISBN: 978-0-470-46547-9 Mix, P. E. Iniewski, K. (ed.) Introduction to Nondestructive Medical Imaging Testing Principles, Detectors, and Electronics A Training Guide Hardcover 2005 ISBN: 978-0-470-39164-8 Hardcover ISBN: 978-0-471-42029-3 Capelo-Martínez, J.-L. (ed.) Hill, C. R., Bamber, J. C., Ultrasound in Chemistry ter Haar, G. R. (eds.) Analytical Applications Physical Principles of Medical 2009 Ultrasonics Hardcover ISBN: 978-3-527-31934-3 Hardcover ISBN: 978-0-471-97002-6 Kundu, T. (ed.) Advanced Ultrasonic Methods for Material and Structure Inspection 2007 Hardcover ISBN: 978-1-905209-69-9 Edited by Roman Gr. Maev Advances in Acoustic Microscopy and High Resolution Imaging From Principles to Applictaions The Editor All books published by Wiley-VCH are carefully produced. Nevertheless, authors, editors, and Prof. Roman Gr. Maev publisher do not warrant the information contained NSERC Indust. Research Chair in these books, including this book, to be free of University of Windsor errors. Readers are advised to keep in mind that 401, Sunset Avenue statements, data, illustrations, procedural details or Windsor ON N9B 3P4 other items may inadvertently be inaccurate. Canada Library of Congress Card No.: applied for British Library Cataloguing-in-Publication Data A catalogue record for this book is available from the British Library. Bibliographic information published by the Deutsche Nationalbibliothek The Deutsche Nationalbibliothek lists this publication in the Deutsche Nationalbibliografie; detailed bibliographic data are available on the Internet at http://dnb.d-nb.de. © 2013 Wiley-VCH Verlag & Co. KGaA, Boschstr. 12, 69469 Weinheim, Germany All rights reserved (including those of translation into other languages). No part of this book may be reproduced in any form – by photoprinting, microfilm, or any other means – nor transmitted or translated into a machine language without written permission from the publishers. Registered names, trademarks, etc. used in this book, even when not specifically marked as such, are not to be considered unprotected by law. Print ISBN: 978-3-527-41056-9 ePDF ISBN: 978-3-527-65533-5 ePub ISBN: 978-3-527-65532-8 mobi ISBN: 978-3-527-65531-1 oBook ISBN: 978-3-527-65530-4 Cover Design Adam-Design, Weinheim, Germany Typesetting Toppan Best-set Premedia Limited, Hong Kong Printing and Binding Markono Print Media Pte Ltd, Singapore V Contents List of Contributors XIII Introduction XVII Author Biographies XIX Part One Fundamentals 1 1 From Multiwave Imaging to Elasticity Imaging 3 Mathias Fink and Mickael Tanter 1.1 Introduction 3 1.2 Regimes of Spatial Resolution 3 1.3 The Multiwave Approach 4 1.4 Wave to Wave Generation 5 1.5 Wave to Wave Tagging 7 1.6 Wave to Wave Imaging: Mapping Elasticity 8 1.7 Super-resolution in Supersonic Shear Wave Imaging 14 1.8 Clinical Applications 16 1.9 Conclusion 19 References 21 2 Imaging via Speckle Interferometry and Nonlinear Methods 23 Jeffrey Sadler and Roman Gr. Maev 2.1 General Introduction 23 2.2 Part I: Speckle Interferometry 24 2.2.1 Introduction 24 2.2.2 Labeyrie’s Method 25 2.2.3 Knox–Thompson Method 29 2.2.4 Importance of Phase Difference Calculation 32 2.2.5 Labeyrie and Knox–Thompson in Two Dimensions 33 2.2.6 Other Improvements to Speckle Interferometry 34 2.3 Part II: Nonlinear Imaging 34 2.3.1 Introduction 34 2.3.2 Deviation (Difference Squared), or Absolute Difference 36 VI Contents 2.3.3 Fourier Transform-Based Methodology 36 2.3.4 Fourier Methodology: How to Create an Image 38 2.3.5 Fourier Transform: Problems with Using 39 2.3.6 Hilbert Transform-Based Methodology 39 2.3.7 Hilbert Methodology: How to Create an Image, and 3D Image 42 2.4 Summary and Closing 44 Selected References (By Subject) 45 Speckle: Base Methods 45 Speckle: More Advanced Methods 45 Nonlinear Imaging 45 Part Two Novel Developments in Advanced Imaging Techniques and Methods 47 3 Fundamentals and Applications of a Quantitative Ultrasonic Microscope for Soft Biological Tissues 49 Kazuto Kobayashi and Naohiro Hozumi 3.1 General Introduction: Basic Idea of an Ultrasonic Microscope for Biological Tissues 49 3.2 Sound Speed Profile 50 3.2.1 Fundamentals 50 3.2.2 Specimen to be Observed 50 3.2.3 Experimental Setup and Acquired Signal 51 3.2.4 Calculation of Sound Speed 52 3.2.4.1 Frequency Domain Analysis 52 3.2.4.2 Time–Frequency Domain Analysis 54 3.2.5 Two-Dimensional Sound Speed Profiles 56 3.2.6 Attempts at Better Spatial Resolution 58 3.3 Acoustic Impedance Profile 60 3.3.1 Fundamentals 60 3.3.2 Experimental Setup 61 3.3.3 Specimen to be Observed 62 3.3.4 Acquired Signal 63 3.3.5 Calibration for Characteristic Acoustic Impedance 63 3.3.6 Observation of Cerebellar Cortex of a Rat 65 3.3.7 Cell Size Observation 67 3.3.8 Commercialized Equipment 69 3.4 Summary 70 References 70 4 Portable Ultrasonic Imaging Devices 71 Sergey A. Titov, Roman Gr. Maev, and Fedar M. Severin References 91 Contents VII 5 High-Frequency Ultrasonic Systems for High-Resolution Ranging and Imaging 93 Michael Vogt and Helmut Ermert 5.1 General Introduction 93 5.2 High-Frequency Ultrasonic System Components 94 5.2.1 Ultrasound Echo Systems 94 5.2.2 Transmitter and Receiver Components for High-Frequency Ultrasonic Echo Systems 95 5.2.3 Spectral and Range Resolution Properties 97 5.2.4 Measurement and Optimization of the Pulse Transfer Properties 99 5.2.5 Range Resolution Optimization: Inverse Echo Signal Filtering 101 5.2.6 Measurement of Acoustic Scattering Parameters in Plane Wave Propagation 102 5.3 Engineering Concepts for High-Frequency Ultrasonic Imaging 104 5.3.1 Single-Element Transducer B-Scan Techniques 104 5.3.2 Lateral Resolution Optimization 105 5.3.2.1 B/D-Scan Technique 106 5.3.2.2 Synthetic Aperture Focusing Techniques (SAFT) 106 5.3.3 Limited Angle Spatial Compounding (LASC) 110 5.3.4 Multidirectional Tissue Characterization 112 5.4 High-Frequency Ultrasound Imaging in Biomedical Applications 115 5.4.1 Skin Imaging 115 5.4.2 Imaging of Small Animals 117 5.5 Summary 118 References 119 6 Quantitative Acoustic Microscopy Based on the Array Approach 125 Sergey Titov and Roman Gr. Maev 6.1 General Introduction 125 6.2 Measurement of Velocity and Attenuation of Leaky Waves 126 6.3 Measurement of Bulk Wave Velocities and Thickness of Specimen 141 6.4 Conclusions 150 References 150 Part Three Advanced Biomedical Applications 153 7 Study of the Contrast Mechanism in an Acoustic Image for Thickly Sectioned Melanoma Skin Tissues with Acoustic Microscopy 155 Bernhard R. Tittmann, Chiaki Miyasaka, Elena Maeva, and David Shum 7.1 Introduction 155 7.1.1 What Is Melanoma? 155 7.1.2 How Is Melanoma Diagnosed? 156 VIII Contents 7.1.3 Present Problems for Biopsy 157 7.1.4 Objective of Present Study 157 7.2 Physical and Mathematical Modeling for Five Layer Wave Propagation in an Acoustic Microscope 158 7.3 Sample Preparation 162 7.4 Digital Imaging – Optical and Ultrasonic 163 7.4.1 Optical Image 163 7.4.2 Acoustic Imaging Principle (Pulse-Wave Mode) 164 7.4.3 Resolution 168 7.4.4 Acoustic Images 169 7.4.5 Waveform Analysis 171 7.5 High Frequency Acoustic Microscopy 174 7.5.1 Normal Control Skin Tissue 174 7.5.2 Abnormal Skin Tissue 175 7.5.3 Acoustic Velocity 175 7.5.4 Computer Simulation 177 7.5.4.1 Experimental V(z) Curve 177 7.5.4.2 Theoretical V(z) Curve (Simulation of V(z) Curve) 178 7.6 Conclusions 181 Acknowledgment 183 References 183 8 New Concept of Pathology – Mechanical Properties Provided by Acoustic Microscopy 187 Yoshifumi Saijo 8.1 Introduction 187 8.2 Principle of Acoustic Microscopy 188 8.3 Application to Cellular Imaging 189 8.4 Application to Hard Tissues 191 8.5 Application to Soft Tissues 193 8.5.1 Gastric Cancer 193 8.5.2 Myocardial Infarction 195 8.5.3 Kidney 197 8.5.4 Atherosclerosis 197 8.6 Ultrasound Speed Microscopy (USM) 200 8.7 Articular Tissues 202 8.8 Summary 202 References 204 9 Quantitative Scanning Acoustic Microscopy of Bone 207 Pascal Laugier, Amena Saïed, Mathilde Granke, and Kay Raum 9.1 Introduction 207 9.1.1 Hierarchical Structure of Bone and Properties 207 9.1.2 Relevance of Multiscale Elastic Properties 209 9.1.3 History of Measurement Principles 210 Contents IX 9.2 Quantitative SAM-Based Impedance of Bone 213 9.2.1 Theory 213 9.2.2 Time-Resolved Measurements 216 9.2.3 Measurements with Time-Gated Amplitude Detection 217 9.2.3.1 Calibration 218 9.3 Tissue Mineralization, Acoustic Impedance, and Stiffness 219 9.4 Elastic Anisotropy at the Nanoscale (Lamellar) Level 222 9.5 Elastic Anisotropy at the Microscale (Tissue) Level 223 9.6 Applications in Musculoskeletal Research 225 9.7 Conclusions 226 References 228 Part Four Advanced Materials Applications 231 10 Array Imaging and Defect Characterization Using Post-processing Approaches 233 Alexander Velichko, Paul D. Wilcox, and Bruce W. Drinkwater 10.1 Introduction 233 10.2 Modeling Array Data 237 10.2.1 Introduction 237 10.2.2 Ray-Based Description of Ultrasonic Array Data 238 10.2.2.1 Determining the Ray-Paths 238 10.2.2.2 Predicting the Signal Associated with a Ray-Path 240 10.2.2.3 Simple Example 240 10.2.3 Mathematical Model of Ultrasonic Array Data 242 10.3 Imaging with 1D Arrays 245 10.3.1 Classical Beam-Forming Imaging Methods in Post-processing 245 10.3.2 Total Focusing Method 246 10.3.3 Wavenumber Method 247 10.3.4 Back-Propagation Method 249 10.3.5 Theoretical Comparison of Imaging Methods 250 10.3.6 Computational Burden 251 10.3.7 Focusing Performance 252 10.3.8 Experimental Example 253 10.4 Imaging with 2D Arrays 255 10.4.1 Optimization of 2D Array Layout 255 10.4.1.1 Optimization Criterion 255 10.4.1.2 Regular Sampling 256 10.4.1.3 Non-uniform Sampling 257 10.4.2 Experimental Comparison of 2D Array Layouts 258 10.4.2.1 Spherical Inclusion 259 10.4.2.2 Aluminum Block with Flat Bottom Holes 260 10.4.2.3 Surface-Breaking Fatigue Crack 260 10.5 Scattering Matrices and Their Experimental Extraction 260 X Contents 10.5.1 Feature Extraction from Array Data 262 10.5.1.1 Concept 262 10.5.1.2 Inverse Imaging 263 10.5.1.3 Extraction of Scattering Matrix 266 10.6 Defect Characterization and Sizing 267 10.6.1 Crack Sizing 267 10.6.1.1 1D Array 267 10.6.1.2 2D Array 268 10.6.2 Experimental Results 269 10.6.2.1 1D Array 269 10.6.2.2 2D Array 271 10.7 Conclusions 272 References 273 11 Ultrasonic Force and Related Microscopies 277 Andrew Briggs and Oleg V. Kolosov 11.1 Introduction 277 11.2 Mechanical Diode Detection 279 11.3 Experimental UFM Implementation 280 11.4 UFM Contrast Theory 283 11.5 Quantitative Measurements of Contact Stiffness 287 11.6 UFM Picture Gallery 289 11.7 Image Interpretation – Effects of Adhesion and Topography 293 11.8 Superlubricity 295 11.9 Defects Below the Surface 297 11.10 Time-Resolved Nanoscale Phenomena 299 Acknowledgments 303 References 304 12 Ultrasonic Atomic Force Microscopy 307 Kazushi Yamanaka and Toshihiro Tsuji 12.1 Introduction 307 12.2 Principle 307 12.2.1 Forced Vibration of Cantilever from the Base 307 12.2.2 Quantitative Information, Directional Control, and Resonance Frequency Tracking 308 12.2.3 Effective Enhancement of Cantilever Stiffness 309 12.2.4 Criterion to Avoid Plastic Deformation 309 12.3 Theory 311 12.3.1 Overview 311 12.3.2 Linear Analysis of Stiffness and the Q Factor 312 12.3.3 Linear Theory of Subsurface Imaging 314 12.3.4 Advantage of Appropriate Load 316 12.3.5 Nonlinear Analysis of Spectra 316 12.3.6 Duffing Model 318