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Skin and seed grape extract as an antioxidant for mechanically deboned chicken meat, during frozen storage Hernán Horacio Tournour Porto, December 2014 Dissertation Thesis Skin and seed grape extract as an antioxidant for mechanically deboned chicken meat, during frozen storage Dissertation presented to Faculty of Nutrition and Food Science to obtain the degree of Doctor of Philosophy (PhD) in Food Consumption and Nutrition Sciences Supervisor: Dr. Luís Miguel Cunha, Associated Professor, FCUP Co-supervisors: Dr. Marcela Alves Segundo, Assistant Professor, FFUP Dr Jorge Bernardo Lacerda de Queiroz, Assistant Professor, FCUP Hernán Horacio Tournour Porto, 2014 Hernán Tournour is grateful for financial support from Erasmus Mundus Action 2 Strand 1 Lot 16, (ET11D0009ER), Argentina Program - Project 2 Eurotango through a PhD Scholarship. The studies presented here were done at REQUIMTE Laboratory for Food Quality and Preservation of Faculty of Sciences of the University of Porto at the Agrarian Campus in Vairão, at REQUIMTE Laboratory of Applied Chemistry of Faculty of Pharmacy of the University of Porto, at the Higher Institute of Engineering of Porto and at SenseTest Lda., Vila Nova de Gaia. Author’s Declaration Under the terms of the “Decreto-lei nº 216/92, de 13 de Outubro” is hereby declared the following original articles were prepared in the scope of this thesis. Under the terms of the referred “Decreto-lei”, the author declares that he afforded a major contribution to the conceptual design and technical execution of the work, interpretation of the results and manuscript preparation of the published articles included in the thesis. This dissertation is constituted by the following papers submitted for publications: I. Hernán H. Tournour, Marcela A. Segundo, Luís M. Magalhães, Luísa Barreiros, Jorge Queiroz, Luís M. Cunha. Valorization of grape pomace: extraction of bioactive phenolics with antioxidant properties. (Submitted for publication). II. Hernán H. Tournour, Marcela A. Segundo, Luís M. Magalhães, Jorge Queiroz, M. Beatriz P. P. Oliveira, Luís M. Cunha. Single and successive oxidative stress factors applied to mechanically deboned chicken meat (MDM): protective effect of grape pomace extract. (Submitted for publication). III. Hernán H. Tournour, Marcela A. Segundo, Luís M. Magalhães, Telmo J. R. Fernandes, M. Beatriz P. P. Oliveira, Luís M. Cunha. Influence of Portuguese grape pomace extracts on the oxidative stability, nutritional and color characteristics of mechanically deboned chicken meat. (Submitted for publication). IV. Hernán H. Tournour, Marcela A. Segundo, Luís M. Magalhães, Anabela S. G. Costa, Luís M. Cunha. Effect of “Touriga nacional” grape extract on quality characteristics of mechanically deboned chicken meat kept under frozen storage. (Submitted for publication). V. Hernán H. Tournour, Luís M. Cunha, Luís M. Magalhães, Rui Costa Lima, Marcela A. Segundo. Evaluation of the joint effect of the incorporation of mechanically deboned meat and grape extract on the formulation of chicken nuggets. (Submitted for publication). vii Moreover, the author declares that he has actively participated in the preparation and writing of each paper, being actively engaged in the stages of experimental design, sample preparation and evaluation, data collection, analysis and interpretation of results. viii Acknowledgements Here there is a small tribute to the institutions and all those people who in one or in other way have brought knowledge, guidance, support and why not friendship during this long journey that comprise a PhD course, University of Porto, Faculty of Nutrition and Food Sciences (FCNAUP) and Faculty of Sciences (FCUP) for accepting me as PhD student, Faculty of Sciences-U.Porto (Lab. Of Food Quality and Preservation at the Agrarian Campus in Vairão), Faculty of Pharmacy-U.Porto (Lab. of Applied Chemistry and Lab. of Bromatology) and Instituto Superior de Engenharia-Porto (Lab. "Grupo de Reação e Análises Químicas") for granting me access to laboratories and facilities where this work was developed, Erasmus Mundus Action 2 Strand 1 Lot 16 Argentina Eurotango2 (ET11D0009ER) for the financial support through a PhD scholarship, Prof. Dr. Maria Daniel Vaz de Almeida, who during her functions as FCNAUP director accepted and gave me the possibility to participate in this fruitful experience as a PhD student, Prof. Dr. Luís Cunha for his support, assistance, willingness, motivation, data analysis assistance and his guidance during this journey of learning, Prof. Dr. Marcela Segundo for her able and concise assistance, I really like to thank her endless patience, scientific advices and mentorship too, Prof. Dr. Jorge Queiroz for his supporting help during the samples selection and provision, Prof. Dr. Beatriz Oliveira for having made possible to work with her research team in the Lab of Bromatology-FFUP, Dr. Luís Magalhães for his accessible communication, having advised and guided during all laboratory activities, Dr. Luísa Barreiros for her help and support provided during the laboratorial activities, ix MSc Anabela Costa and MSc Telmo Fernandes for all the help and support during my laboratorial activities at REQUIMTE / Food Science Service, All my colleagues from the different laboratories (REQUIMTE, Faculty of Pharmacy, and Vairão) in which I developed my activities and experiences, I am thankful for providing me a very comfortable atmosphere, Miss Anabela Borges for her solidarity and kindness, SenseTest staff, especially Eng. Rui Costa Lima, for having provided the place in which to develop the activities regarding sensorial analyses, Avicasal Poultry Industry and Quinta do Crasto wine farm for having supplied the chicken meat samples and grape pomace, respectively, My family, for strong bases and values that have managed to convey; and love, that is the reason that everything worthwhile in life, All my friends from Argentina and Portugal; life takes another sense when you have their company and support, God because nothing could be possible without His help, To all above I would to express my sincerest gratitude, many thanks! x

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Doctor of Philosophy (PhD) in Food Consumption and Nutrition Sciences declared the following original articles were prepared in the scope of this thesis. Dr. Maria Daniel Vaz de Almeida, who during her functions as FCNAUP analysis assistance and his guidance during this journey of learning,.
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