Silkworm biofactory : silk to biology PDF
Preview Silkworm biofactory : silk to biology
Silkworm Biofactory Silk to Biology Editors Enoch Y. Park Institute of Green Science & Technology Shizuoka University Shizuoka, Japan Katsumi Maenaka Hokkaido University Sapporo, Japan p, p, A SCIENCE PUBLISHERS BOOK A SCIENCE PUBLISHERS BOOK CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2(cid:19)0(cid:17)1(cid:18)9(cid:26)(cid:1)by Taylor & Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Printed on acid-free paper Version Date:(cid:1)20180820 International Standard Book Number-13: (cid:1)9(cid:1)(cid:1)7(cid:1)(cid:1)8(cid:1)(cid:1)(cid:1)-(cid:1)1-138-32812-9(cid:1) (cid:1)(Hardback) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, includ- ing photocopying, microfilming, and recording, or in any information storage or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please access www.copyright.com (http://www.copyright.com/) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of users. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe. Visit the Taylor & Francis Web site at http://www.taylorandfrancis.com and the CRC Press Web site at http://www.crcpress.com Preface Silkworms have been used for human needs for centuries. The silk derived from the silkworm has of great economic value and recently has used as value-added biomaterials for biopharmaceutical purpose. Silkworms have attracted the attention for expressing eukaryotic recombinant proteins, which require post-translational modifications. In 1985 Dr. Maeda demonstrated that silkworm larvae could produce a functional human α-interferon. Since then, to express recombinant proteins in silkworm various technologies have been developed. Baculovirus-based expression system (BES) comes foremost and has been used in for a long time. Baculovirus being the ideal vector carrying the desired cDNA of interest does not infect nor cause any known disease to humans or other livestock other than its host. The BES was suitable for expressing recombinant proteins in insect cells using Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, in the case of the silkworm, since Bombyx mori nucleopolyhedrovirus (BmNPV) has limited host cells and genetic manipulation kits, silkworm has not been easily used for protein production using BmNPV, but mainly silk production. Today’s biotechnology-based industry using silkworm faces a bottleneck towards further advancement owing to the lack of proper gene expression systems. However, with the development of BmNPV bacmid system, being capable of replicating in both Escherichia coli and Bombyx mori-derived cell lines or silkworm, silkworm larvae or pupae have been used for expression system for the recombinant protein production. This method utilizes the advantage of a bacmid it can be easily prepared and screened in E. coli to produce sufficient DNA for subsequent expression in silkworms. This would be a great breakthrough in the production of recombinant eukaryotic proteins and viruses, which will be a powerful tool in a new proteome era. This volume provides comprehensive up to date review about recent and future trends of silkworm biotechnology and potential pharmaceutical applications, aiming at contributing in the field of life science and bioindustry. This volume consisted of three parts. In Part I Silkworm biology and physiology, genetics, breeding, posttranslational iv Silkworm Biofactory: Silk to Biology modification and glycoprotein pathway of silkworm are dealt in Chapters 1 to 6. In Part II Utilization of silkworm as a biofactory, new expression system, transgenic silkworm, expression of membrane proteins, virus-like particles and gene delivery are described in Chapters 7 to 13. In Part III Bioproducts from silkworm, bioproducts or the recombinant proteins from silkworm that nearby may be commercialized are introduced in Chapters 14 to 17. This volume begins with an overview of the fundamental research about the silkworm biofactory and extended to the wide applications for the large-scale expression of foreign genes. Contents Preface iii Part I Silkworm Biology and Physiology 1. Basic and Applied Genetics of Silkworms 3 Toru Shimada 2. Silkworm Breeding 18 Yuyin Chen 3. Focus on Physiological Vital Proteins from Silkworm 38 Bombyx mori Raman Chandrasekar and S. K. M. Habeeb 4. Metabolism of Molting Hormone and Juvenile 62 Hormone in Silkworm Yungen Miao 5. Post-translational Modification in Silkworm 88 Sungjo Park, Enoch Y. Park and Andre Terzic 6. Glycoprotein Biosynthesis of Silkworm 107 Kazuhito Fujiyama Part II Utilization of Silkworm as a Biofactory 7. BacMam System in Silkworm 129 Takashi Tadokoro 8. Transgenic Silkworm 143 Hideki Sezutsu 9. Glycosyltransferase Expression in Silkworm and 163 its Applications in Glycobiology Makoto Ogata, Taichi Usui and Enoch Y. Park 10. Silkworm Expression of Cell Surface Molecules 180 Shunsuke Kita vi Silkworm Biofactory: Silk to Biology 11. Expression and Purification of Mitochondrial Membrane 193 Protein OPA1 for Reconstitution of Membrane Fusion Tadato Ban and Naotada Ishihara 12. Virus-like Particles Expression in Silkworms 204 Vipin Kumar Deo and Enoch Y. Park 13. Gene Delivery Based on Bombyx mori 220 Nucleopolyhedrovirus (BmNPV) Tatsuya Kato and Enoch Y. Park Part III Bioproducts from Silkworm 14. Development of New Biomaterials from Insect and its Virus 235 Eiji Kotani and Hajime Mori 15. Production of Medical and Cosmetic Products using 251 Transgenic Silkworms Masahiro Tomita 16. Comparative Investigation of Influenza Virus-like Particles 266 from Synthetic Chemical Substance and Silkworm Kuniaki Nerome, Kazumichi Kuroda and Shigeo Sugita 17. Production of Feline and Canine Interferon from Silkworm 287 Tsuyoshi Tanaka and Takashi Tanaka Index 299 Part I Silkworm Biology and Physiology 1 C hapter Basic and Applied Genetics of Silkworms Toru Shimada 1. Introduction Genomics is the basis for all researches, and no research in modern biology and biotechnology can be performed without genomic information. Currently the whole-genome sequences of several organisms are being made public and can be obtained from public databases for use in biological research. The first draft of the genome sequence of Bombyx mori (strain p50T), the domesticated silkworm, was published in 2004 by the National Institute of Agrobiological Sciences in collaboration with other Japanese researchers (Mita et al. 2004). In the same year, a Chinese group independently published their draft sequence of B. mori strain Dazao, which was originally classified as the same strain as p50T. Later, both groups collaborated and exchanged raw sequence data in order to eventually produce a high- accuracy genome sequence containing new gene models (International Silkworm Genome Consortium 2008). The silkworm genome analysis is a major contribution to basic biology. The silkworm is a representative insect of the order Lepidoptera (moths and butterflies), and the first species of the Lepidopterans to have a completely sequenced genome. Its genomic information is valuable not only for comparative genomic studies on insects and animals but also as the basis of studies on biological phenomena, such as morphogenesis, complicated pigmentation patterns and metamorphosis, unique to Laboratory of Insect Genetics and Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. E-mail: [email protected]
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