role for hydrogen peroxide during abiotic and biotic stress signaling in plants PDF
Preview role for hydrogen peroxide during abiotic and biotic stress signaling in plants
GHENT UNIVERSITY FACULTY OF SCIENCES – DEPARTMENT MOLECULAR GENETICS VIB DEPARTMENT OF PLANT SYSTEMS BIOLOGY ROLE FOR HYDROGEN PEROXIDE DURING ABIOTIC AND BIOTIC STRESS SIGNALING IN PLANTS KORNEEL VANDENBROUCKE Thesis submitted in partial fulfillment of the requirements for the degree of Doctor (Ph.D.) of Science, Biotechnology Academic year 2007-2008 Promoters: Prof. Dr. Frank Van Breusegem Prof. Dr. Dirk Inzé Printed by Plot-It, BVBA Cover: Artwork done by Armée de verre (Steven Theunis) The scientist is not a person who gives the right answers, he's one who asks the right questions Claude Lévi-Strauss Exam Committee EXAM COMMITTEE Promoters Prof. Dr. Frank Van Breusegem (secretary) Department of Molecular Genetics, Ghent University, Belgium VIB Department of Plant Systems Biology Prof. Dr. Dirk Inzé Department of Molecular Genetics, Ghent University, Belgium VIB Department of Plant Systems Biology Promotion Committee Prof. Dr. Ann Depicker (chairman) Department of Molecular Genetics, Ghent University, Belgium VIB Department of Plant Systems Biology Prof. Dr. Yves Van De Peer Department of Molecular Genetics, Ghent University, Belgium VIB Department of Plant Systems Biology Dr. Ir. Gerrit Beemster Department of Molecular Genetics, Ghent University, Belgium VIB Department of Plant Systems Biology Prof. Dr. Ir. Monica Höfte Department of Crop Protection Laboratory of Phytopathology, Faculty of Bio-engineering, Ghent University, Belgium Dr. Michael Metzlaff Bayer BioScience N.V., Ghent Belgium Prof. Dr. Graham Noctor Institut de Biotechnologie des Plantes, Université de Paris, France Prof. Dr. Yves Guisez Department of Biology Faculty of Sciences Antwerp University, Belgium 5 Table of Contents TABLE OF CONTENTS Exam committee 5 Table of contents 7 List of abbreviations 9 Research Objectives 13 PART I GENERAL INTRODUCTION Chapter 1 15 How do plants deal with abiotic stress? Meta-analysis on transgenic plants with increased stress tolerance Abstract Introduction Conclusions and Perspectives References PART II HYDROGEN PEROXIDE SIGNALING IN PLANTS Chapter 2 49 Hydrogen peroxide-induced gene expression across kingdoms: A comparative analysis Abstract Introduction Results and Discussion Conclusions Materials and Methods References Chapter 3 69 Identification of genes involved in plant defense against necrotrophic pathogens by using virus- induced gene silencing Abstract Introduction Results Discussion Conclusions and Perspectives Materials and Methods References Chapter 4 97 Ectopic expression of the WRKY15 transcription factor in Arabidopsis increases tolerance to oxidative stress Abstract Introduction Results Discussion Conclusions and Perspectives Materials and Methods References 7 Table of Contents PART III DROUGHT STRESS IN ARABIDOPSIS Chapter 5 123 Development and evaluation of a semi-automated system to monitor the growth of Arabidopsis plants during drought stress Abstract Introduction Results Discussion Conclusions and Perspectives Materials and Methods References Supplementary data Chapter 6 145 Molecular phenotyping of drought tolerant Arabidopsis plants overexpressing a galactinol synthase, GOLS2, reveals a role for myo-inositol Abstract Introduction Results and Discussion Perspectives Materials and Methods References PART IV GENERAL DISCUSSION AND SUMMARY OF THE THESIS Chapter 7 163 General Discussion, Conclusions and Perspectives Chapter 8 167 Summary / Samenvatting PART V ADDENDUM Supplementary Tables 181 Curriculum Vitae 205 Dankwoord 207 8 List of Abbreviations LIST OF ABBREVIATIONS 26S RSU 26S proteasome regulatory subunit A ABA Abscisic acid AFLP Amplified fragment length polymorphism AGI Arabidopsis Genome Initiative Agrobacterium Agrobacterium tumefaciens APX Ascorbate peroxidase Arabidopsis Arabidopsis thaliana ATP Adenine triphosphate B BLAST Basic local alignment and search tool C CAT Catalase cDNA Complementary DNA Col Columbia ecotype of Arabidopsis thaliana D DNA Deoxyribonucleic acid DRP DNA-afhankelijk RNA polymerase DW Dry Weight E EST Expressed sequence tag F FC Fold Change FW Fresh Weight G GO Gene ontology GPX Glutathione peroxidise GST Gene-specific tag GTP Guanine triphosphate H H O Hydrogen peroxide 2 2 HRM H O -response matrix 2 2 HSP Heat shock protein M MAPK Mitogen-activated protein kinase MI Myo-inositol mRNA Messenger RNA O OE Overexpression P PCR Polymerase chain reaction PDS Phyotene desaturase Ph.D. Phylosophiae doctor POF Protein with obscure features R RNA Ribonucleic acid ROS Reactive oxygen species S SDR Short-chain dehydrogenase/reductase SOD Superoxide dismutase STG Stress tolerance gene T T-DNA Transferred DNA TF Transcription factor ThrRS Threonyl tRNA synthetase tRNA Transfer RNA TRV Tobacco rattle virus U Ub Ubiquitin V VIGS Virus-induced gene silencing W WIWAM Weighing imaging and watering machine WT Wild type 9
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