RESIDUAL ANTIBIOTICS IN MARKETABLE MILK IN KHARTOUM STATE By Rasha Ali Omer Ali

77 Pages·2006·0.25 MB·English

RESIDUAL ANTIBIOTICS IN MARKETABLE MILK IN
KHARTOUM STATE

By

Rasha Ali Omer Ali
(B.V.Sc. 2000, University of Bahr El Ghazal)

Supervisor:

Dr. Tawfig El Tigani Mohammed

A thesis submitted in partial fulfillment for the requirements of
Master of Tropical Animal Health (M.T.A.H)

Department of Preventive Medicine and Veterinary Public Health, Faculty
of Veterinary Medicine, University of Khartoum

January, 2006
To my parents,

My brothers & sisters
PREFACE

This study has been done in the Department of Preventive Medicine and Pubic
Health, Faculty
of Veterinary Medicine, University of Khartoum
Under the supervision of
Dr. Tawfig El Tigani Mohammed
ACKNOWLEDGEMENTS

I would like to thank my supervisor Dr.Tawfig El Tigani for his guidance,
and close and keen supervision throughout this work.
I am deeply grateful to Prof. Khalil Abd Al Gabbar due to his endless
help and fruitful advices throughout this study that made the work easea.
I would like to express my sincere thanks to Dr. Abd Al Wahab A.
Mahmoud for his continuous help and encouragement during this study.
My thanks are also extended to Amna Al Tillib due to her help and
provision of references.
Thanks are extended to Dr. Adil Suliman and Dr. Atif Alamin , for their
help and advices throughout this work.
Thanks are also extended to Dr. Amir Siddig and Badur Farah for their technical
support .
Thanks to Dr. Husein Abd Alla, Dr. Intissar Al Ashi, Dr. Hind Al Nasri
and Dr. Mohammed Abd Al moniem for their help.
ABSRARACT

Milk spoilage remains a problems in many countries especially those in
tropics with absence of preservation practices such as pasteurization . this
has tempted milk dealers to use antimicrobial substances to combat the
problem of spoilage with consequence health hazards. Khartoum State
may not an exception as field observations suggested the use of
antibiotics to preserve milk under the high ambient temperature of the
day. Therefore the current study has been carried out in Khartoum State
to investigate this possibility through culturing a strain of Bacillus
subtilles (strain ATTC 6633) of no previously reported exposure to
antibiotics in Nutrient Agar media. Wells were punched in the Nutrient
Agar. Then, these wells filled with individual milk samples. Two hundred
and sixty milk samples were collected randomly from three provinces of
Khartoum State. These samples were collected from two different
sources, the first source was conducted from 41 farms, the second source
was collected from market milk and were collected both from the
morning and the evening milking.
The result indicated that antimicrobial substances, possibly antibiotics, are widely used
to preserve milk sold in Khartoum State. Percentages of 27.1%, 21.2% and 25% of the
total milk samples collected from Khartoum, Khartoum North and Omdurman were
respectively positive for antimicrobial residues.
Further, higher percentage of samples collected from Khartoum markets was positive
(30.9%) compared with those collected directly from producer farms (24.3%). Also
higher percentage of samples collected in the morning (38.9%) compared with those
collected in the evening (16.8%) suggesting an attempt to protect milk from the higher
possibility of spoilage due to the high day temperature.
The study has therefore recommended measures to combat the practice of
adding antimicrobial substances to milk though random sampling and
legislating regulations and rules, including penalties, to control the
practice. Also milk producers should be encouraged to form cooperatives
that should be provided with pasteurization, bottling and distribution
facilities. In addition, door to door dispersion of milk should only be
restricted to pasteurized and bottled milk.
ﺔﺣوﺮﻃﻷا ﺺﺨﻠﻣ

بﺎ(cid:1127)(cid:1127)ﻴﻏ ﻊ(cid:1127)(cid:1127)ﻣ ةرﺎ(cid:1127)(cid:1127)ﺤﻟا ﻖﻃﺎ(cid:1127)(cid:1127)ﻨﻤﻟا ﻲ(cid:1127)(cid:1127)ﻓ ﺔ(cid:1127)(cid:1127)ﺻﺎﺧ رﺎ(cid:1127)(cid:1127)ﻄﻗﻷا ﻦ(cid:1127)(cid:1127)ﻣ ﺮ(cid:1127)(cid:1127)ﻴﺜآ ﻲ(cid:1127)(cid:1127)ﻓ ﺔﻠﻜﺸ(cid:1127)(cid:1127)ﻣ ﻦﺒ(cid:1127)(cid:1127)ﻠﻟا دﺎﺴ(cid:1127)(cid:1127)ﻓ ﻞﻜﺸ(cid:1127)(cid:1127)ﻳ
ﺔ(cid:1127)ﻄﺒﺜﻣ داﻮ(cid:1127)ﻣ ماﺪﺨﺘ(cid:1127)ﺳﻻ ﻦﺒﻠﻟا ﻲﻟواﺪﺘﻣ ﻦﻣ ﺮﻴﺜﻜﻟا ﺖﻋد ﺔﻠﻜﺸﻤﻟا ﻩﺬه ،ةﺮﺘﺴﺒﻟﺎآ ﻆﻔﺤﻟا قﺮﻃ ماﺪﺨﺘﺳا
. نﺎ(cid:1127)ﺒﻟﻷ ا ﻩﺬ(cid:1127)ه ﻲﻜﻠﻬﺘﺴ(cid:1127)ﻤﻟ ﺔﻴﺤ(cid:1127)ﺻ ﻞآﺎﺸ(cid:1127)ﻣ ﻚ(cid:1127)ﻟذ ﻊﺒﺘﻳ ﺎﻤﻣ ﻦﺒﻠﻟا دﺎﺴﻓ ﺔﻠﻜﺸﻣ ﻰﻠﻋ ﺐﻠﻐﺘﻠﻟ ﻚﻟذ ،ﻢﻴﺛاﺮﺠﻠﻟ
تادﺎﻀ(cid:1127)(cid:1127)ﻤﻟا ماﺪﺨﺘ(cid:1127)(cid:1127)ﺳا ﻦ(cid:1127)(cid:1127)ﻣ ﺔ(cid:1127)(cid:1127)ﻴﻠﻘﺤﻟا تﺎ(cid:1127)(cid:1127)ﻈﺣﻼﻤﻟا ﻪ(cid:1127)(cid:1127)ﻴﻟإ ﺮﻴﺸ(cid:1127)(cid:1127)ﺗ ﺎ(cid:1127)(cid:1127)ﻣ ﻦ(cid:1127)(cid:1127)ﻋ ﺔﻴﻨﺜﺘﺴ(cid:1127)(cid:1127)ﻤﺑ ﺖﺴ(cid:1127)(cid:1127)ﻴﻟ مﻮ(cid:1127)(cid:1127)ﻃﺮﺨﻟا ﺔ(cid:1127)(cid:1127)ﻳﻻو
ﺪ(cid:1127)(cid:1127)ﻗ ﺔ(cid:1127)(cid:1127)ﺳارﺪﻟا ﻩﺬ(cid:1127)(cid:1127)ه نﺈ(cid:1127)(cid:1127)ﻓ اﺬ(cid:1127)(cid:1127)ﻬﻟ .مﻮ(cid:1127)(cid:1127)ﻴﻟا لﻼ(cid:1127)(cid:1127)ﺧ ةﺪﺋﺎﺴ(cid:1127)(cid:1127)ﻟا ﺔ(cid:1127)(cid:1127)ﻴﻟﺎﻌﻟا ةراﺮ(cid:1127)(cid:1127)ﺤﻟا ﺔ(cid:1127)(cid:1127)ﺟرد ﺖ(cid:1127)(cid:1127)ﺤﺗ ﻦﺒ(cid:1127)(cid:1127)ﻠﻟا ﻆ(cid:1127)(cid:1127)ﻔﺤﻟ ﺔ(cid:1127)(cid:1127)ﻳﻮﻴﺤﻟا
ﺎ (cid:1127)(cid:1127)ﻳﺮﺘﻜﺒﻟا ﻦ(cid:1127)(cid:1127)ﻣ ةﺮ(cid:1127)(cid:1127)ﺘﻋ ﻊ(cid:1127)(cid:1127)ﻳرﺰﺗ لﻼ(cid:1127)ﺧ ﻦ(cid:1127)(cid:1127)ﻣ ﻚ(cid:1127)(cid:1127)ﻟذو مﻮ(cid:1127)(cid:1127)ﻃﺮﺨﻟا ﺔ(cid:1127)(cid:1127)ﻳﻻو ﻲ(cid:1127)ﻓ لﺎ(cid:1127)(cid:1127)ﻤﺘﺣﻻا اﺬ(cid:1127)(cid:1127)ه ﻲﺼ(cid:1127)(cid:1127)ﻘﺗ ﻰ(cid:1127)(cid:1127)ﻟإ ﺖﻓﺪ(cid:1127)ه
رﺎ(cid:1127)(cid:1127)ﺟﺁ ﻂ(cid:1127)(cid:1127)ﺳو ﻲ(cid:1127)(cid:1127)ﻓ ﻞ(cid:1127)(cid:1127)ﺒﻗ ﻦ(cid:1127)(cid:1127)ﻣ ﺔ(cid:1127)(cid:1127)ﻳﻮﻴﺣ تادﺎﻀ(cid:1127)(cid:1127)ﻤﻟ ﺎﻬ(cid:1127)(cid:1127)ﺿﺮﻌﺗ ﻞﺠﺴ(cid:1127)(cid:1127)ﻳ ﻢ(cid:1127)(cid:1127)ﻟ ﻲ(cid:1127)(cid:1127)ﺘﻟا 6633 ﺔﻳﺰﻴﻠﺗﺎﺴ(cid:1127)(cid:1127)ﻟا ﺔﻳﻮﺼ(cid:1127)(cid:1127)ﻌﻟا
ﺔ(cid:1127)ﻳدﺮﻔﻟا ﻦﺒ(cid:1127)ﻠﻟا تﺎ(cid:1127)ﻨﻴﻌﺑ ﺮ(cid:1127)ﻔﺤﻟا ﻩﺬ(cid:1127)ه ﺊ(cid:1127)ﻠﻣ ﻢ(cid:1127)ﺗ ﻢ(cid:1127)ﺛ ،ﻲﺋاﺬ(cid:1127)ﻐﻟا رﺎ(cid:1127)ﺟﻵا ﺢﻄ(cid:1127)ﺳ ﻰﻠﻋ ﺮﻔﺣ ﺖﻌﻨﺻ ﻢﺛ . ﻲﺋاﺬﻏ
ﺖ(cid:1127)ﻌﻤﺟ تﺎ(cid:1127)ﻨﻴﻌﻟا ﻩﺬ(cid:1127)ه ،مﻮ(cid:1127)ﻃﺮﺨﻟا ﺔ(cid:1127)ﻳﻻو ﻦ(cid:1127)ﻣ ﺎًﻴﺋاﻮﺸﻋ ﺖﻌﻤﺟ ﻦﺒﻟ ﺔﻨﻴﻋ 260 ﺎﻬﻠﻤﺠﻣ ﻲﻓ ﺖﻧﺎآ ﻲﺘﻟاو
قاﻮ(cid:1127)(cid:1127)ﺳأ ﻦ(cid:1127)(cid:1127)ﻣ ﻊ(cid:1127)(cid:1127)ﻤﺟ ﻲﻧﺎ(cid:1127)(cid:1127)ﺜﻟا رﺪﺼ(cid:1127)(cid:1127)ﻤﻟاو ﺔ(cid:1127)(cid:1127)ﻋرﺰﻣ 41 ﻦ(cid:1127)(cid:1127)ﻣ نﺎ(cid:1127)(cid:1127)آ لوﻷا رﺪﺼ(cid:1127)(cid:1127)ﻤﻟا ،ﻦﻴ(cid:1127)(cid:1127)ﻔﻠﺘﺨﻣ ﻦﻳرﺪﺼ(cid:1127)(cid:1127)ﻣ ﻦ(cid:1127)(cid:1127)ﻣ
،ﻢﻴﺛاﺮ(cid:1127)ﺠﻠﻟ ﺔ(cid:1127)ﻄﺒﺜﻣ داﻮ(cid:1127)ﻣ نأ ﺚ(cid:1127)ﺤﺒﻟا ﻲﻓ ﺎﻬﻴﻠﻋ ﻞﺼﺤﺘﻟا ﻢﺗ ﻲﺘﻟا ﺞﺋﺎﺘﻨﻟا ﺖﺤﺿوأ .ء ًﺎﺴﻣو ﺎًﺣﺎﺒﺻ ﻦﺒﻠﻟا
ذإ مﻮ(cid:1127)ﻃﺮﺨﻟا ﺔ(cid:1127)ﻳﻻو ﻲ(cid:1127)ﻓ ﻦﻴﻜﻠﻬﺘﺴ(cid:1127)ﻤﻠﻟ عﺎ(cid:1127)ﺒﻳ يﺬ(cid:1127)ﻟا ﻦﺒ(cid:1127)ﻠﻟا ﻆ(cid:1127)ﻔﺤﻟ ﺎﻬﻟﺎﻤﻌﺘﺳا دﻮﺴﻳ ﺔﻳﻮﻴﺣ تادﺎﻀﻣ ﺎﻤﺑرو
قاﻮ(cid:1127)ﺳأ ﻦ(cid:1127)ﻣ ﺖ(cid:1127)ﻌﻤﺟ ﻲ(cid:1127)ﺘﻟا ﻦﺒ(cid:1127)ﻠﻟا تﺎ(cid:1127)ﻨﻴﻋ ﻞ(cid:1127)ﻤﺠﻣ ﻦ(cid:1127)ﻣ %25 %21.2 %27.1 ﻦ(cid:1127)ﻣ ح واﺮ(cid:1127)ﺘﺗ ﺐﺴﻨﻟا نأ
داﻮ(cid:1127)(cid:1127)ﻤﻟا ﻩﺬ(cid:1127)ه ﺎ(cid:1127)ﻳﺎﻘﺒﻟ ﻲﻟاﻮ(cid:1127)(cid:1127)ﺘﻟا ﻰ(cid:1127)ﻠﻋ ﺔ(cid:1127)ﺒﺟﻮﻣ ﺖ(cid:1127)(cid:1127)ﻧﺎآ نﺎﻣرﺪ(cid:1127)ﻣأو يﺮ(cid:1127)ﺤﺑ مﻮ(cid:1127)(cid:1127)ﻃﺮﺨﻟا ، مﻮ(cid:1127)ﻃﺮﺨﻟا ﻦ(cid:1127)ﻣ نﺎ(cid:1127)ﺒﻟﻷا
قاﻮ(cid:1127)ﺳأ ﻦ(cid:1127)ﻣ ﺖ(cid:1127)ﻌﻤﺟ ﻲ(cid:1127)ﺘﻟا تﺎ(cid:1127)ﻨﻴﻌﻟا ﻦ(cid:1127)ﻣ ﺔ(cid:1127)ﻴﻟﺎﻋ ﺔ(cid:1127)ﻳﻮﺌﻣ ﺐﺴ(cid:1127)ﻧ كﺎ(cid:1127)ﻨﻬﻓ ﻚ(cid:1127)ﻟذ ﻰ(cid:1127)ﻟإ ﺔﻓﺎ(cid:1127)ﺿإ ﻢﻴﺛاﺮﺠﻠﻟ ﺔﻄﺒﺜﻤﻟا
عراﺰ(cid:1127)(cid:1127)ﻤﻟا ﻦ(cid:1127)(cid:1127)ﻣ ةﺮ(cid:1127)(cid:1127)ﺷﺎﺒﻣ ﺖ(cid:1127)(cid:1127)ﻌﻤﺟ ﻲ(cid:1127)(cid:1127)ﺘﻟا ﻦﺒ(cid:1127)(cid:1127)ﻠﻟا تﺎ(cid:1127)(cid:1127)ﻨﻴﻋ ﻦ(cid:1127)(cid:1127)ﻣ (%24.3) ـ(cid:1127)(cid:1127)ﺑ ﺔ(cid:1127)(cid:1127)ﻧرﺎﻘﻣ (%30.9) نﺎ(cid:1127)(cid:1127)ﺒﻟﻷا
(%38.9) حﺎﺒﺼ(cid:1127)ﻟا ﻲ(cid:1127)ﻓ ﺖ(cid:1127)ﻌﻤﺟ ﻲ(cid:1127)ﺘﻟا ﻦﺒﻠﻟا تﺎﻨﻴﻋ ﻲﻓ ﺎﻬﻠﻴﺠﺴﺗ ﻢﺗ ﺔﻴﻟﺎﻋ ﺔﺒﺴﻧ كﺎﻨه نأ ﺎﻤآ .ﺔﺠﺘﻨﻤﻟا
لﺎ(cid:1127)ﻤﺘﺣﻻا ﻦ(cid:1127)ﻣ ﻦﺒ(cid:1127)ﻠﻟا ﺔ(cid:1127)ﻳﺎﻤﺤﻟ ﺔ(cid:1127)ﻟوﺎﺤﻣ ﻰ(cid:1127)ﻟإ ﺮﻴﺸ(cid:1127)ﻳ ﺎ(cid:1127)ﻤﻣ (%16.8) ءﺎﺴ(cid:1127)ﻣ ﺖ(cid:1127)ﻌﻤﺟ ﻲ(cid:1127)ﺘﻟا ﻚ(cid:1127)ﻠﺗ ﻊ(cid:1127)ﻣ ﺔﻧرﺎﻘﻣ
.مﻮﻴﻟا لﻼﺧ ﺔﻴﻟﺎﻌﻟا ةراﺮﺤﻟا ﺔﺟرﺪﻟ ﺔﺠﻴﺘﻧ ﻒﻠﺘﻠﻟ ﺮﻴﺒﻜﻟا
ﺔ(cid:1127)(cid:1127)ﻄﺒﺜﻤﻟا داﻮ(cid:1127)(cid:1127)ﻤﻟا ﺔﻓﺎ(cid:1127)(cid:1127)ﺿإ ﺔﻠﻜﺸ(cid:1127)(cid:1127)ﻣ ﻰ(cid:1127)(cid:1127)ﻠﻋ ﺐ(cid:1127)(cid:1127)ﻠﻐﺘﻟا ﻰ(cid:1127)(cid:1127)ﻟإ فﺪ(cid:1127)(cid:1127)ﻬﺗ تﺎﻴ(cid:1127)(cid:1127)ﺻﻮﺗ ﻰ(cid:1127)(cid:1127)ﻟإ ﺔ(cid:1127)(cid:1127)ﺳارﺪﻟا ﺖﺼ(cid:1127)(cid:1127)ﻠﺧ
ﻚ(cid:1127)(cid:1127)ﻟذ ﻲ(cid:1127)(cid:1127)ﻓ ﺎ(cid:1127)(cid:1127)ﻤﺑ ﻂﺑاﻮ(cid:1127)(cid:1127)ﺿو ﺲ(cid:1127)(cid:1127)ﺳأو ﻦﻴﻧاﻮ(cid:1127)(cid:1127)ﻗ ﻊ(cid:1127)(cid:1127)ﺿوو ﺔﻴﺋاﻮﺸ(cid:1127)(cid:1127)ﻋ تﺎ(cid:1127)(cid:1127)ﻨﻴﻋ ﺬ(cid:1127)(cid:1127)ﺧأ لﻼ(cid:1127)(cid:1127)ﺧ ﻦ(cid:1127)(cid:1127)ﻣ ﻚ(cid:1127)(cid:1127)ﻟذو ﻢﻴﺛاﺮ(cid:1127)(cid:1127)ﺠﻠﻟ
ﻦﻳﻮ(cid:1127)(cid:1127)ﻜﺘﻟ نﺎ(cid:1127)(cid:1127)ﺒﻟﻷا ﻲ(cid:1127)(cid:1127)ﺠﺘﻨﻣ ﻊﻴﺠﺸ(cid:1127)(cid:1127)ﺘﻟ ﺎﻀ(cid:1127)(cid:1127)ﻳأ ﺚ(cid:1127)(cid:1127)ﺤﺒﻟا اﺬ(cid:1127)(cid:1127)ه ﻰ(cid:1127)(cid:1127)ﺻوأ ﺎ(cid:1127)(cid:1127)ﻤآ ﺔ(cid:1127)(cid:1127)ﺳرﺎﻤﻤﻟا ﻩﺬ(cid:1127)(cid:1127)ه ﻦ(cid:1127)(cid:1127)ﻣ ﺪ(cid:1127)(cid:1127)ﺤﻠﻟ تﺎ(cid:1127)(cid:1127)ﺑﻮﻘﻋ
ﺪ(cid:1127)(cid:1127)ﻘﻓ ﻚ(cid:1127)(cid:1127)ﻟذ ﻰ(cid:1127)(cid:1127)ﻟإ ﺔﻓﺎ(cid:1127)(cid:1127)ﺿإ . ﻊ(cid:1127)(cid:1127)ﻳزﻮﺘﻟاو ﺔ(cid:1127)(cid:1127)ﺌﺒﻌﺘﻟاو ةﺮﺘﺴ(cid:1127)(cid:1127)ﺒﻟا تﺎ(cid:1127)(cid:1127)ﻴﻧﺎﻜﻣﺈﺑ تﺎ(cid:1127)(cid:1127)ﻴﻧوﺎﻌﺘﻟا ﻩﺬ(cid:1127)(cid:1127)ه ﺪ (cid:1127)(cid:1127)ﻤﺗ نأو تﺎ(cid:1127)(cid:1127)ﻴﻧوﺎﻌﺗ
.ﻪﻨﻣ ﺄﺒﻌﻤﻟاو ﺮﺘﺴﺒﻤﻟا ﻦﺒﻠﻟا ﻰﻠﻋ اًﺮﺼﺣ لزﺎﻨﻤﻟا ﻰﻟإ ﻦﺒﻠﻟا ﻊﻳزﻮﺗ نﻮﻜﻳ نأ ﻰﻟإ ﺔﺳارﺪﻟا ﺖﺻوأ
INTRODUCTION
Milk has played a major contribution to the human nutrition in many
different countries a cross the world. It is the neonatal food of animals and
man, and provides practically all the ingredients necessary to promote and
maintain life .
Cow’s milk represents about 90.8% of the world milk production with
buffalo, sheep, and goats producing 6%, 1.7% and 1.5% respectively
(Harding & Ditton,1995).
Antibiotics are applied as one of the most important chemical agents in the
dairy farms as therapeutics , controlling infections, and as growth
promoter .
Some countries report that more than 50% of their total output of
antimicrobial compounds is used in agriculture (WHO, 1997).
These advantages of antibiotics usage have been associated with
significant contamination from a wide variety of antibiotics residues
introduced via treatment of the cow; thus rendering milk and meat unfit
for human consumption ,and it should be protected against direct or
indirect contact with any source of contamination.
People all over the world's major dairy producing countries are concerned
with the quality and safety of milk. These concerns are generated by
pressure from consumers, processors, and the international market. Hence,
consumers are increasingly demanding high quality foods that are
wholesome, nutritious, and safe. Processors are aware that antibiotic
residues may result in economical and industrial problems in
manufacturing produced milk product. As the international trade in dairy
products increases, governments need to know the quality and safety of
imported products that meets or exceeds their internal requirements.
Due to the consumers demand worldwide for improved food safety, the
OIE is working with other relevant organizations in reducing food-borne
risks to human health including hazards that involve chemical residues in
food with the potential harmful health effect in humans (OIE, 2005).
In 1969 the US Food and drug administration issued a warning that the use
of antibiotics in food-producing animals should be regulated more closely
owing to the potential health hazard from residues in milk, meat, and eggs
(WHO,1970).
However, in many countries especially those in the tropics such as Sudan,
milk spoilage remains a problem in absence of preservation practices such
as pasteurization. This has tempted milk dealers to use antimicrobial
substances to combat the problem of spoilage with the consequent health
hazards. Sudan may not be an exception as field observations suggested
the use of antibiotics to preserve milk under the high ambient temperate of
the day. Therefore the current study has been carried out to investigate this
possibility through culturing a strain of Bacillus subtilles of no previously
reported exposure to antibiotics in nutrient agar media.
Objective of the study:
As based on the forgoing introduction the aim of this research is:-
1- To detect the presence of antibiotics residues in raw milk in
Khartoum State market and dairy farms in order ascertain whether it
is safe for human consumption or not .
2- The effect of temperature on the frequency of antibiotics in milk.