Repeated Measures ANOVA Prof. Wei Zhu Department of Applied Mathematics & Statistics Stony Brook University 2 The One-way ANOVA we have just learnt can test the equality of several population means. It is an extension of the pooled variance t-test That is: H (null hypothesis) : µ = µ = µ =…….. = µ 0 1 2 3 k H (alternative hypothesis): At least one of a means differs from the rest. Assumptions: Unknown but equal population variances Normal populations Independent samples 3 The F statistic k 2 1 n x x k1 i i F i1 n k j 2 1 x x Nk ij i i1 j1 where x = the jth observation in the i th sample. ij i 1,2,,k and j 1,2,,n i n i x ij x j1 mean for ith sample i 1,2,,k i n i k n i k x ij N n Total sample size i i1 j1 x Overall mean i1 N 4 The ANOVA table Source S.S d.f, M.S. F k MS Between SS n x x2 MS 1 k n x x2 F B B i i k 1 MS B k1 i i i1 W i1 k nj k nj Within SS x x 2 MS 1 x x 2 N k W ij i W Nk ij i i1 j1 i1 j1 The ANOVA table is a tool for displaying the computations for the F test. It is very important when the Between Sample variability is due to two or more factors. We reject the ANOVA null hypothesis of equal means if F > F k-1,N-k, α 5 Limitations of the one-way ANOVA: The most distinct disadvantage to the analysis of variance (ANOVA) method is that it requires three assumptions to be made: ☼ The samples must be independent to each other. ☼ All variances from each data group, though unknown, must be equal.The normality assumption. 6 7 ☼ A repeated measures design is one in which at least one of the factors consists of repeated measurements on the same subjects or experimental units, under different conditions and/or at different time points. 8 A repeated measures design often involves measuring subjects at different points in time – or subjects measured under different experimental conditions It can be viewed as an extension of the paired-samples t-test (which involved only two related measures) Thus, the measures—unlike in the “regular” ANOVA—are correlated, that is, the observations are not independent 9 Example: Data collected in a sequence of (often) evenly spaced points in time – this is usually referred to as the ‘longitudinal data’ Example: Different treatments are assigned to the each experimental unit 10
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