Regulation of cytokine production in the rat osteoblast by transforming growth factor-BETA

308 Pages·1992·11.6 MB·English

by  Phillips, John L

UNIVERSITY LIBRAR'J
390020108402
!
1. Phillips
YALE
MEDICAL LIBRARY
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REGULATION OF CYTOKINE
PRODUCTION IN THE RAT OSTEOBLAST
BY
TRANSFORMING GROWTH FACTOR-BETA
A thesis submitted to the Yale University School of Medicine
in Partial Fulfillment of the Requirements
for the Degree of Doctor of Medicine.
by John L. Phillips
1992
NEW HAVEN, CONNECTICUT MARCH, 1992

TABLE OF CONTENTS
Acknowledgments.i
Photo, xerograph, and illustration credits.ii
Glossary and Abbreviations.iii
I. ABSTRACT.1
II. INTRODUCTION.3
A. Transforming Growth Factor-B.9
B. Interleukin-1.20
C. Interleukin-6.35
The Colony Stimulating Factors.41
D. Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF).43
E. Macrophage Colony Stimulating Factor (M-CSF).48
F. Thesis Investigation.52
III. MATERIALS AND METHODS.53
A. Cell preparation.53
B. Preparation of Conditioned Media.54
C. Cytokine assays.55
D. RNA analysis.60
IV. RESULTS.64
A. Studies of GM-CSF.64
TGF-B and LPS.64
TGF-B and PTH.72
TGF-B and IL-1.73