Redox Cell Biology and Genetics Part A

616 Pages·2002·22.133 MB·English

Contributors to Volume 352
Article numbers are in parentheses following the names of conU'ibutors.
Affiliations listed are cun'ent.
HUDA AKin (25), Mental Health Research DNANIDREF C. BREEDVELD (21), Depart-
Institute, University of Michigan, Ann ment of Rheumatology, Leiden Univer-
Arbor, Michigan 48109 sity Medical Center, 2300 RC Leiden, The
Netherlands
ROBERT E. ANDERSON (40), Department of
Neurosurgery, Thoralf M. Sundt, .rJ Neu-
RAOUL BREITKREUTZ (20), Department
rosurgery Research Laboratory, Mayo
of Immunochemistry, Deutsches Krebs-
Clinic, Rochester, Minnesota 55905
forschungszentrum, D-69120 Heidel-
ROSANNA ANZEVINO (8), Institute of berg, Germany
General Pathology, Catholic University,
00168 Rome, Italy OIDUALC A. OqIAMAAC (25), Mental Health
Research Institute, University of Michi-
MUSTAFA ATALAY (36), Department of gan, Ann Arbor, Michigan 48109
Physiology, University of Kuopio, FIN-
70211 Kuopio, Finland ANTONI CAMINS (6), Pharmacology and
Pharmacognosy Unit, Universitat de
E1LAHTAN AUG~ (5), INSERM U-466, In-
Barcelona, 08028 Barcelona, Spain
stitut Louis Bugnard IFR 31, 31403
Toulouse Cedex 4, France
BRITTON CHANCE (39), Department of Bio-
SUSANNE BACHER (4), Department of chemistry and Biophysics, University
Immunochemistry, German Cancer Re- of Pennsylvania School of Medicine,
search Center, D-69120 Heidelberg, Philadelphia, Pennsylvania 19104
Germany
PEEDVAN S. CHANDEL (2), Department
H/JLYA BAY1R (30), Departments of Anes- of Medicine, Northwestern University
thesiology and Critical Care Medicine,
Medical School, Chicago, Illinois 60611
University of Pittsburgh, Pittsburgh,
Pennsylvania 15260 NILOC F. CHIGNELL (3), Laboratory of
Pharmacology and Chemistry, National
BARBARA BEDOGNI (8), Institute of General
Institute of Environmental Health Sci-
Pathology, Catholic University, 00168
ences, National Institutes of Health, Re-
Rome, Italy
search Triangle Park, North Carolina
HERV~ BENOIST (5), INSERM U-466, In- 27709
stitut Louis Bugnard IFR 31, 31403
Toulouse Cedex 4, France OTREBILIF ONIM1C (22), Department of Bio-
chemistry and Medical Biotechnology,
PIOTR BILSKI (3), Laboratory of Pharma-
tgtisrevinU di Napoli Federico IL 80131
cology and Chemistry, National Institute
Naples, Italy
of Environmental Health Sciences, Na-
tional Institutes of Health, Research Tri-
THOMAS L. CLANTON (26), Department of
angle Park, North Carolina 27709
Internal Medicine, Pulmonary and Criti-
SILVlA BORRELLO (8), Institute of General cal Care Medicine, Davis Heart and Lung
Pathology, Catholic University, 00168 Research Institute, Ohio State University,
Rome, Italy Columbus, Ohio 43210
ix
x CONTRIBUTORS TO VOLUME 352
MARIE-VERONIQUE TNEMELC (13), Depart- NEGRJ./J FUCHS (28), Department of Derma-
ment of Biochemistry, National Univer- tology, Johann Wolfgang Goethe Univer-
sity of Singapore and National University sity, 60590 Frankfurt am Main, Germany
Medical Institutes, Singapore 117597
SHOJI FUKUDA (34), Cardiovascular Re-
RENATA ITT1VALOC (8), Institute of General search Center, University of Connecticut
Pathology, Catholic University, 00168 School of Medicine, Farmington, Con-
Rome, Italy necticut 06030
LEAHCIM E CZUBRYT (11), Division TOMMASO GALEOTrI (8), Institute of
of Stroke and Vascular Disease, .tS General Pathology, Catholic University,
Boniface General Hospital Research 00168 Rome, Italy
Centre, Winnipeg, Manitoba, Canada GAYLE M. GORDILLO (36), Department
R2H 2A6 of Surgery, Laboratory of Molecular
Medicine, Davis Heart and Lung Re-
MARGARET E. DAUB (3), Department of
Botany, North Carolina State University, search Institute, Ohio State University,
Columbus, Ohio 43210
Raleigh, North Carolina 27695
AGNES GORLACH (19), Department of
HERALDO E ED SOUZA (37), Emergency
Experimental Pediatric Cardiology,
Medicine Department, University of ot~S
German Heart Center Munich, Techni-
Paulo School of Medicine, CEP 05403-
cal University Munich, 80636 Munich,
000 ot~S Paulo, Brazil
Germany
NEIL DONOGHUE (9), Center for Throm-
SONJA I. GRINGHUIS (21), Department of
bosis and Vascular Research, School of
Rheumatology, Leiden University Medi-
Pathology, University of New South
cal Center, 2300 RC Leiden, The Nether-
Wales, 2052 Sydney, Australia
lands
WULF DROGE (4), Department oflmmuno-
NORBERT GROTH (28), FOM Institute, TFH
chemistry, German Cancer Research
Berlin, University of Applied Science and
Center, D-69120 Heidelberg, Germany
Technology, 12489 Berlin, Germany
CARINE DURAL (5), INSERM U-466, In- DRAHNREB HENNIG (18), Cell Nutrition
stitut Louis Bugnard IFR 31, 31403
Group, College of Agriculture, University
Toulouse Cedex 4, France
of Kentucky, Lexington, Kentucky 40546
NAITSIRHC E. ELGER (12), Department of CHRISTIAN HERFARTH (20), Department
Epileptology, University of Bonn Medical of Surgery, University of Heidelberg,
Center, D-53105 Bonn, Germany D-69120 Heidelberg, Germany
FRANCA OTISOPSE (22), Department of Bio- OLAF HERKERT (19), Institute for Car-
chemistry and Medical Biotechnology, diovascular Physiology, Johann Wolf-
Universita di Napoli Federico IL 80131 gang Goethe University Hospital, 60590
Naples, Italy Frankfurt am Main, Germany
JAMES E FABISIAK (14), Department of En- THOMAS HERRLrNG (28), FOM Institute,
vironmental and Occupational Health, TFH Berlin, University of Applied Sci-
University of Pittsburgh, Pittsburgh, ence and Technology, 12489 Berlin,
Pennsylvania 15260 Germany
HPLODNAR S. FAUSTINO (11), Division of PHILIP J. HOGG (9), Center for Thrombosis
Stroke and Vascular Disease, .tS Boniface and Vascular Research, School of Pathol-
General Hospital Research Centre, Win- ogy, University of New South Wales, 2052
nipeg, Manitoba, Canada R2H 2A6 Sydney, Australia
CONTRIBUTORS TO VOLUME 352 xi
CARL A. HUBEL (30), Magee Womens Re- OCSICNARF R. M. ODN1RUAL (37), ralucsaV
search Institute and Department of -bO Biology and Applied Physiology Labora-
stetrics, Gynecology and Reproductive ,seirot Heart Institute, University of Sao
,secneicS University of ,hgrubsttiP Pitts- Paulo School of Medicine, CEP 05403-
,hgrub Pennsylvania 15260 000 ot~S ,oluaP Brazil
OR1HUZAK ICHIKAWA (41), Department of EEHGNUJ LEE (16), Department of -lorueN
,scisyhpoiB Graduate School of Phar- ,ygo Harvard Medical School, Boston,
maceutical Sciences, Kyushu ,ytisrevinU sttesuhcassaM 02115
,uk-ihsagiH Fukuoka ,2858-218 Japan
YONG OOW LEE (18), Department of
MARIANO IKSWEZSINAJ (37), Vascular -iB ,yregruS Division of ,yregrusorueN Uni-
ology and Applied Physiology Labora- versity of Kentucky Medical ,retneC
,seirot Heart Institute, University of odS ,notgnixeL Kentucky 40536
Paulo School of Medicine, PEC 05403-
000 ot~S ,oluaP Brazil BIAO LIu (10), Department of yrtsimehC
and ,yrtsimehcoiB Queens College of the
NAIRELAV E. KAGAN (14, 30), Depart- City University of New ,kroY ,gnihsulF
ment of Environmental and Occupa- New York 11367
tional Health, University of ,hgrubsttiP
,hgrubsttiP Pennsylvania 15260 SHANG-XI LIU (30), Department of En-
vironmental and Occupational Health,
SIMONE KAISER (18), Department of ytisrevinU of Pittsburgh, Pittsburgh,
,yregruS Division of ,yregrusorueN Uni- ainavlysnneP 15260
versity of Kentucky Medical ,retneC
,notgnixeL Kentucky 40536 MANUEL O. L6PEZ-FIGUEROA (25),
Pritzker Neuropsychiatric Disorders
PETER KAYATZ (33), Laboratory of -repxE Research Consortium, San ,ocsicnarF
imental ,ygolomlahthpO University Eye ainrofilaC 11149
,cinilC University of ,engoloC D-50931
,engoloC Germany G. MIKE SOGROIGIRKAM (7), Department
of Adult Oncology, Dana-Farber Can-
SAVITA KHANNA (17, 27), Departments cer Institute, Harvard Medical ,loohcS
of Surgery and Molecular and Cellular ,notsoB Massachusetts 02115
,yrtsimehcoiB Laboratory of Molecular
Medicine, Davis Heart and Lung Re- SEMAJ C. MALIAKAL (15), BD Bio-
search Institute, Ohio State ,ytisrevinU sciences Discovery Labware, Bedford,
,submuloC Ohio 43210 sttesuhcassaM 0371O
NOREMAC J. KOCH (1), Department of MASAYUKI MATSUDA (39), Department of
Radiation ,ygolocnO University of -nneP ,yregrusorueN Shiga University of Med-
sylvania, Philadelphia, Pennsylvania ical Science, Ohtsu, Shiga 520-2192,
40191 napaJ
DAGMAR KUNZ (12), Institute of lacinilC OR1HCI--NEK MATSUMOTO (41), Showa
yrtsimehC and ,yrtsimehcoibohtaP Med- College of Pharmaceutical Sciences,
ical Center of RWTH Aachen, 52072 ,adihcaM Tokyo ,3458-491 Japan
Aachen, Germany
MARK .P MATTSON (38), Laboratory of
WOLFRAM S. KUNZ (12), Department of Neurosciences, Gerontology Research
,ygolotpelipE ytisrevinU of Bonn Medical ,retneC National Institute on Aging, Bal-
,retneC D-53105 Bonn, Germany ,eromit Maryland 21224
xii CONTRIBUTORS TO VOLUME 352
GAUTAM MAULIK (7), Department of Adult RACHEL L. NEVE (16), Department of Psy-
Oncology, Dana-Farber Cancer Insti- chiatry, Harvard Medical School, Boston,
tute, Harvard Medical School, Boston, Massachusetts 02115
Massachusetts 02115
TERRY D. OBERLEY (32), Department of
NILANJANA MAULIK (34), Cardiovascular Pathology and Laboratory Medicine,
Research Center, University of Connecti- University of Wisconsin Medical School,
cut School ofMedicine, Farmington, Con- and William S. Middleton Memorial Vet-
necticut 06030 erans Administration Hospital, Madison,
AIHTNYC J. MEININGER (24), Cardiovascu- Wisconsin 53 705
lar Research Institute and Department
TETSUO OI (41), Imaging Research Labo-
of Medical Physiology, Texas A&M Uni-
ratories ,I Daiichi Radioisotope Labora-
versity System Health Science Center,
tories, Ltd., Matsuo-machi, Chiba 289-
Temple, Texas 76504
1592, Japan
STEFAN MEUER (20), Institute of Immunol-
ogy, University of Heidelberg, D-69120 MERC~ PALLAS (6), Pharmacology and
Heidelberg, Germany Pharmacognosy Unit, Universitat de
Barcelona, 08028 Barcelona, Spain
FREDRIC B. MEYER (40), Department of
Neurosurgery, Thoralf M. Sundt, .rJ Neu- GIOVANNI PAN (8), Institute of General
rosurgery Research Laboratory, Mayo Pathology, Catholic University, 00168
Clinic, Rochester, Minnesota 55905 Rome, Italy
MICHAEL .V MmKIN (10), Department of MARCELO DE A. PEDRO (37), Vascular Bi-
Chemistry and Biochemistry, Queens ology and Applied Physiology Laborato-
College of the City University of New ries, Heart Institute, University of S~o
,kroY Flushing, New York 11367 Paulo School of Medicine, CEP 05403-
M. IN~S MORANO (25), Mental Health Re- 000 ot~S Paulo, Brazil
search Institute, University of Michigan,
BIZAHS PERVAIZ (13), Department of Phys-
Ann Arbor, Michigan 48109
iology, National University of Singapore,
LucIA MORB1DELLI (35), Institute of Singapore 117597
Pharmacological Sciences, University of
Siena, 53100 Siena, Italy GRANT N. PIERCE (11), Division of Stroke
and Vascular Disease, .tS Boniface Gen-
AZlZA MWIDAU (16), Department of Neu- eral Hospital Research Centre, Winnipeg,
rology, Harvard Medical School, Boston, Manitoba, Canada R2H 2A6
Massachusetts 02115
RAJIV R. RATAN (16), Department of Neu-
MASAICHI NARUSE (41), Imaging Research
,ygolor Harvard Medical School, Boston,
Laboratories ,I Daiichi Radioisotope
Massachusetts 02115
Laboratories Ltd., Matsuo-machi, Chiba
289-1592, Japan
FANNY ROBBESYN (5), INSERM U-466,
DANI NAZZAL (5), INSERM U-466, Institut Institut Louis Bugnard IFR 31, 31403
Louis Bugnard IFR 31, 31403 Toulouse Toulouse Cedex 4, France
Cedex 4, France
SUSAN A. ROTENBERG (10), Depart-
ANNE NEGRE-SALVAYRE (5), Department ment of Chemistry and Biochemistry,
of Biochemistry, INSERM U-466, Institut Queens College of the City Univer-
Louis Bugnard IFR 31, 31403 Toulouse sity of New York, Flushing, New York
Cedex 4, France 11367
CONTRIBUTORS TO VOLUME 352 xiii
ITAWHSAS ROY (17, 27, 36), Departments CHANDAN K. SEN (17, 27, 36), Depart-
of Surgery and Molecular and Cellular ments of Surgery and Molecular and -leC
,yrtsimehcoiB Laboratory of Molecular lular ,yrtsimehcoiB Laboratory of Mo-
Medicine, Davis Heart and Lung Re- lecular Medicine, Davis Heart and Lung
search Institute, Ohio State ,ytisrevinU Research ,etutitsnI Ohio State ,ytisrevinU
,submuloC Ohio 43210 ,submuloC Ohio 43210
OSAMMOT RUSSO (22), Department of -oiB AKIHIKO SHIINO (39), Department of Neu-
chemistry and Medical ,ygolonhcetoiB ,yregrusor Shiga University of Medical
tgtisrevinU di Napoli Federico ,II 80131 ,ecneicS Ohtsu, Shiga ,2912-025 Japan
,selpaN Italy ANNA A. AVODEVHS (14), Department of
latnemnorivnE and lanoitapuccO ,htlaeH
HOON RYU (16), Department of ,ygolorueN
ytisrevinU of Pittsburgh, Pittsburgh,
Harvard Medical School, Boston, Mas-
ainavlysnneP 15260
sttesuhcas 02115
BERND S~O (20), Department of ,yregruS
RAVI SALGIA (7), Department of Adult ytisrevinU of ,grebledieH D-69120 Hei-
,ygolocnO Dana-Farber Cancer ,etutitsnl ,grebled Germany
Harvard Medical School, Boston, Mas-
sttesuhcas 02115 INDERJIT SINGH (31), Medical ytisrevinU
of South Carolina, Charleston, South
TREBOR ERYAVLAS (5), Department of Bio- aniloraC 29425
,yrtsimehc INSERM ,664-U Institut Louis
YROGERG D. SLOOP (29), Department of
Bugnard IFR ,131 31403 esuoluoT Cedex
,ygolohtaP Louisiana State University
,4 France
School of Medicine, New Orleans,
ERDNAXELA VOLIUOMAS (42), Department Louisiana 21107
of Medicine, Johns Hopkins ytisrevinU CSECNARF X. SUREDA (6), Pharmacology
School of ,enicideM ,eromitlaB Maryland ,tinU Universitat Rovira i ,iligriV 43201
42212 Reus ,)anogarraT( Spain
HIROAKI SANO (41), Imaging Research Lab- JEAN-CLAUDE SRE1HT (5), INSERM ,664-U
oratories L Daiichi Radioisotope Labo- Institut Louis Bugnard IFR ,13 31403
,seirotar Ltd., ,ihcam-oustaM Chiba 289- esuoluoT Cedex ,4 France
,2951 Japan
GABRmLE THUMANN (33), Laboratory of
IKAOR1H SASAKI (34), ralucsavoidraC Re- Experimental ,ygolomlahthpO ytisrevinU
search ,retneC University of tucitcennoC Eye Clinic, University of Cologne, D-
School of Medicine, Farmington, Con- 50931 ,engoloC Germany
necticut 06030 MICHAL TOBOREK (18), Department of
,yregruS Division of ,yregrusorueN Uni-
M. LIENHARD ZT1MHCS (4), Department of
versity of Kentucky Medical ,retneC
yrtsimehC and ,yrtsimehcoiB ytisrevinU
,notgnixeL Kentucky 40536
of Bern, CH-3012 Bern, Switzerland
VLADrMm A. TYURrN (14, 30), Depart-
ULRICH REYEMREARHCS (33), Laboratory ment of Environmental and Occupa-
of Experimental ,ygolomlahthpO -revinU tional Health, University of ,hgrubsttiP
sity Eye Clinic, University of ,engoloC ,hgrubsttiP Pennsylvania 15260
D-50931 ,engoloC Germany
YULIA .Y TYURrNA (14, 30), Department
CORNELIA SEEL (20), Department of of Environmental and Occupational
,yregruS University of Heidelberg, D- Health, University of Pittsburgh, Pitts-
69120 Heidelberg, Germany ,hgrub Pennsylvania 15260
xiv CONTRIBUTORS TO VOLUME 352
HIDEO UTSUMI (41), Laboratory of Bio- CORNELIS L. VERWErJ (21), Department
function Analysis, Graduate School of of Molecular Cell Biology, Vrije Uni-
Pharmaceutical Sciences, Kyushu Uni- versiteit Medical Center, 1081 BT
versity, Higashi-ku, Fukuoka 812-8582, Amsterdam, The Netherlands
Japan
YELNATS J. WATSON (25), Mental Health
Research Institute, University of Michi-
RETEP J. A. NAV NED BROEK (23), De-
gan, Ann Arbor, Michigan 48109
partment of Molecular Cell Biology,
Sylvius Laboratory, Leiden University KmSTIN WINKLER (12), Department of Neu-
Medical Center, 2333 AL Leiden, The rology, University of Magdeburg Medical
Netherlands Center, D-39120 Magdeburg, Germany
GUOYAO Wu (24), Departments of Animal
ADNALOJ NAV RED ZEE (23), Department of
Science and Medical Physiology, Texas
Molecular Cell Biology, Sylvius Labora-
A&M University System Health Science
tory, Leiden University Medical Center,
Center, College Station, Texas 77843
2333 AL Leiden, The Netherlands
MARINA ZICHE (35), Institute of Pharma-
MARTrJN M. VANDUrJN (23), Institute for cological Sciences, University of Siena,
Atomic and Molecular Physics, Foun- 53100 Siena, Italy
dation for Fundamental Research on
LI Zuo (26), Department of Internal Med-
Matter, 1098 SJ Amsterdam, The
icine, Pulmonary and Critical Care Medi-
Netherlands
cine, Davis Heart and Lung Research
Institute and Biophysics Program, Ohio
M~KA VENOJARVI (17, 27), Departments
State University, Columbus, Ohio 43210
of Surgery and Molecular and Cellular
Biochemistry, Laboratory of Molecular JAY L. ZWE1ER (42), Department of
Medicine, Davis Heart and Lung Re- Medicine, Johns Hopkins University
search Institute, Ohio State University, School of Medicine, Baltimore, Maryland
Columbus, Ohio 43210 21224
Preface
Oxidants may serve as cellular messengers. Changes in oxidoreductive or
redox status in the cell regulate several signal transduction pathways. Redox-
sensitive changes in signal transduction processes translate to functional changes
at the cellular, tissue, as well as organ levels.
Redox changes in biological cells, tissues, and organs are often transient. For
years, investigators have been challenged by the lack of reliable techniques to
assess such changes in intact biological samples. Only recently have novel cell
biology and genetic techniques to visualize and document redox changes in intact
cells become available. Unlike biochemical methods that rely on the study of bio-
logical extracts, these cell biology- and genetics-related techniques arrest transient
redox changes in the intact cell, tissues, and even organs. Technologies dependent
on laser illumination, advanced spectroscopy, DNA microarray, and related ap-
proaches allow visualization of redox changes in the intact biological sample.
Such approaches, including but not limited to redox imaging of intact organs, gene
therapy, gene screening, flow cytometry and advanced microscopy, represent the
"cutting-edge" technology currently available to only select laboratories.
Our objective was to compile detailed protocols describing and critiquing es-
sential methods in the field of redox cell biology and genetics. Redox Cell Biology
and Genetics, Parts A and B, Volumes 352 and 353 of Methods in ,ygolomyznE
feature a diverse collection of novel cell biology and genetic protocols authored by
highly recognized leaders in the field. Part A covers cellular responses and tissues
and organs; Part B covers structure and functions of proteins and nucleic acids and
genes.
The excellent editorial assistance of Dr. Savita Khanna and the outstanding
contributions of the authors are gratefully acknowledged. We hope that this volume
will contribute to the further development of this important field of biomedical
research.
NADNAHC K. NES
RETSEL REKCAP
XV
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb YENDIS .P KCIWOLOC DNA NAHTAN 0. NALPAK
EMULOV II. Preparation and Assay of Enzymes
Edited yb YENDIS .P KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited yb YENDIS R KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
Edited yb YENDIS R KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV g. Preparation and Assay of Enzymes
Edited yb YENDIS R KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
Edited yb YENDIS R KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VII. Cumulative Subject Index
Edited yb YENDIS R KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VIII. Complex Carbohydrates
Edited yb HTEBAZILE .F DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited yb DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited yb C. H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV xlg. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV xg. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited yb HTENNEK NITSUK
iivx
xviii SDOHTEM NI YGOLOMYZNE
EMULOV XVII. Metabolism of Amino Acids and Amines (parts A and B)
Edited yb TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVI11. Vitamins and Coenzymes (Parts A, B, dna )C
Edited yb DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited yb EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part )C
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb MAILLIW B. YBOKAJ
EMULOV XXI11. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI11. Complex Carbohydrates (Part B)
Edited yb ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part )E
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited yb NHOJ M. NIETSNEWOL
METHODS IN ENZYMOLOGY xix
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XXXlX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited yb JOEL G. NAMDRAH DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited yb W. A. DOOW
EMULOV XLI. Carbohydrate Metabolism (Part C)
Edited yb W. A. DOOW
EMULOV XL11I. Antibiotics
Edited yb NHOJ H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited yb SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited yb MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV aLIa. Enzyme Structure (Part G)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited yb ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited yb AICIRTAP a. EEFFOH DNA MARY NELLE SENOJ
EMULOV LI. Biomembranes (Part C: Biological Oxidations)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIII. Biomembranes (part D: Biological Oxidations)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (part E: Biological Oxidations)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP