Recombinant DNA and Genetic Experimentation. Proceedings of a Conference on Recombinant DNA, Jointly Organised by the Committee on Genetic Experimentation (COGENE) and the Royal Society of London, Held at Wye College, Kent, UK, 1–4 April, 1979 PDF
Preview Recombinant DNA and Genetic Experimentation. Proceedings of a Conference on Recombinant DNA, Jointly Organised by the Committee on Genetic Experimentation (COGENE) and the Royal Society of London, Held at Wye College, Kent, UK, 1–4 April, 1979
RECOMBINANT DNA AND GENETIC EXPERIMENTATION Proceedings of α Conference on Recombinant DNAJointly organised by tlie Committee on Genetic Experimentation! (COGENE) and The Royal Society of London, held at Wye College, Kent UK 1-4 April 1979 Editors JOAN MORGAN and W. J. WHELAN Published for COGENE by PERGAMON PRESS OXFORD · NEW YORK · TORONTO · SYDNEY · PARIS • FRANKFURT U.K. Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW, England U.S.A. Pergamon Press Inc., Maxwell House, Fairvlew Park, Elmsford, New York 10523, U.S.A. CANADA Pergamon of Canada, Suite 104,150 Consumers Rood, Willowdale, Ontario M2J ΊΡ9, Canada AUSTRALIA Pergamon Press (Aust.) Pty. Ltd., P.O. Box 544, Potts Point, N.S.W. 2011, Australia FRANCE Pergamon Press SARL, 24 rue des Ecoles, 75240 Paris, Cedex 05, France FEDERAL REPUBLIC Pergamon Press GmbH, 6242 Kronberg-Taunus, OF GERMANY Pferdstrasse 1, Federal Republic of Germany Copyright ©1979 Pergamon Press Ltd. All Rights Reserved. No part of this publication may be reproduced, stored in α retrieval system or transmitted in any form or by any means: electronic, electrostatic, magnetic tape, mechanical, photocopying, recording or otherwise, without permission in writing from the publishers. First edition 1979 British Library Cataloguing in Pubiication Data Recombinant DNAand genetic experimentation. 1. Recombinant DNA - Congresses I. Morgan, Joan II. Whelaa William Joseph III. Committee on Genetic Experimentation IV. Royal Society 574.8732 QH442 79-40962 ISBN 0-08-024427-0 In order to make this volume available as economically and as rapidly as possible the authors' typescripts have been reproduced in their origlrxal forms. This method has its typographical limitations but it is hoped that they in no way distract the reader. Printed in Great Britain by A. Wheaton & Co., Ltd., Exeter PREFACE The Royal Society and COGENE (The Conmiittee on Genetic Experimentation) were the joint sponsors of an international Conference on recombinant DNA which was held at Wye College, Kent, UK between 1-4 April, 1979. In the belief that the papers presented, and the ensuing discussions, will be of interest to a much wider audience than could be at Wye College, the proceedings are being published on behalf of COGENE. The Conference was called to review the current status of recombinant DNA research and to discuss matters such as the scientific advances in, and practical applications of, recombinant DNA research, the conjectural hazards and the regulation of the research through guidelines and legislation. The Conference was attended by 150 scientists and other participants from more than 30 nations. All the sessions were recorded and the master tapes have been given to the Recombinant DNA History Project at the Massachusetts Institute of Technology, Cambridge^ Mass., USA, to which there will be free access. During the meeting the discussions following the lectures were transcribed and made available for the participants to check and clarify where necessary. Where substantial changes or additions were made, the fact has been noted on the text. These proceedings are grouped into the sessions as they occurred at the meeting and comprise, in the main, a chairman's introduction, a summary of the lecture, and the entire discussion following a lecture or lectures. Our arrangement with the publishers is to achieve a rapid publication and we thank the speakers for prompt submission of their manuscripts and those who participated in the discussions for their cooperation in quickly checking and returning their comments. We would also like to thank Wye College and in particular their Audio Visual Unit for recording the sessions. Special gratitude is due to Carolyn Ison and Linda Reading for transcribing the tapes at the meeting and again to Carolyn Ison for putting these into their final manuscript form and assembling the typescripts. Joan Morgan W.J. Whelan PARTICIPANTS HARIJA ALACEVIC, Faculty of Technology, University of Zagreb, Laboratory of Industrial Microbiology, 41000 Zagreb, Yugoslavia I. ALTOSAAR, Biochemistry Department, University of Ottawa, Ottawa, Ontario, Canada L.J. ARCHER, Molecular Genetics Group, Gulbenkian Institute of Science, Oeiras, Portugal K. AUNSTRUP, NOVO Research Institute, NOVO ALLE, DK-2880, Bagsvaerd, Denmark A.A. BAYEV (COGENE), Institute of Molecular Biology, Academy of Sciences, 32 Vavilov Street, Moscow 117312, USSR G. BERNARDI (COGENE), I.R.B.M,, CNRS, Universite Paris VII - Tour 43, 2 Place Jussieu, 75221 Paris Cedex 05, France G. BERTANI, Institute of Microbial Genetics, Karolinska Institutet, S-104 01 Stockholm, Sweden J.H. BIRKNER, 5008 Alta Loma Road, Colorado Springs, Colorado 80918, USA J. BIRNBAUM, Merck Institute for Therapeutic Research, PO Box 2000, Rahway, New Jersey 07065, USA F, BLASI, Centro di Endocrinologia e Oncologia, Sperimentale del CNR, Via S. Pansini 5, 80131 Naples, Italy W.F. BODMER, Genetics Laboratory, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK R. BREATHNACH, Laboratoire de Genetique Moleculaire des Eukaryotes du CNRS, Faculte de Medecine, 11 Rue Humann, 67085 Strasbourg Cedex, France F. BROWN, Animal Virus Research Institute, Pirbright, Woking, Surrey, UK A, CAMPBELL, Department of Biological Sciences, Stanford University, Stanford, California 94305, USA R. CAPE, Cetus Corporation, 600 Bancroft Way, Berkeley, California 94710, USA A.R. CASHMORE, Royal Society of New Zealand, c/o Applied Biochemistry Division, DSIR Private Bag, Palmerston North, New Zealand S. CHANG, Cetus Corporation, Recombinant Molecular Research, 600 Bancroft Way, Berkeley, California 94710, USA T-Y CHOW, Institute of Botany, Academia Sinica, Taipei, Taiwan 115 XI Participants PATRICIA CLARKE, Department of Biochemistry, University College London, Gower Street, London WCIE 6BT, UK S.N. COHEN (COGENE), Department of Genetics, Stanford University, Room S-337, Stanford, California 94305, USA J.D. COOMBES, Hoechst Pharmaceutical Research Laboratories, Walton Manor, Milton Keynes, Bucks. MK17 9?J, UK P.E. CORNELIS, Universite Catholique de Louvain, Laboratoire de Cytogenetique, Institute Carnoy, 4 Place Croix du Sud, 1348 Louvain-la-Neuve, Belgium R.A. COX, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 lAA, UK R.K. CRAIG, Courtauld Institute of Biochemistry, Middlesex Hospital Medical School, Mortimer Street, London WIP 7PN, UK J. DAVISON, Unit of Molecular Biology, International Institute of Cellular and Molecular Pathology, Avenue Hippocrate 75, B-1200 Brussels, Belgium V. DEBABOV, Institute of Genetics & Selection of Industrial Microorganisms, Dorozhnaya 8, Moscow 113545, USSR S.D. EHRLICH, Institut de Recherche en Biologie Moleculaire, Universite Paris VII, 2 Place Jussieu, 75221 Paris, France I. FEDORCSAK (COGENE), Division of Scientific Research and Higher Education, UNESCO House, 7 Place de Fontenoy, 75700 Paris, France W. FIERS, Laboratory of Molecular Biology, Ledeganekstraat 35, B-9000 Ghent, Belgium MAGDALENA FIKUS, Institute of Biochemistry & Biophysics, Polish Academy of Sciences, Rakowiecka 36, 02-532 Warsaw, Poland J.R.S. FINCHAM, Department of Genetics, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, Scotland, UK S.W. FISHER, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK R.B. FLAVELL, Plant Breeding Institute, Trumpington, Cambridge CB2 2LQ, UK I.I. FODOR, Institute of Biochemistry & Physiology of Microorganisms, Pushchino 142292, Moscow, USSR M, FRIED, Imperial Cancer Research Fund Laboratories, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK A. FURUYA, Tokyo Research Laboratory, Kyowa Hakko Kogyo Co. Limited, 3-6-6 Asahicho, Machidashi, Tokyo 194, Japan Participants W.J. GARTLAND, Director, Office of Recombinant DNA Activities, NIGMS, Building 31, Room 4A52, Bethesda, Maryland 20014, USA T. GEFFEN, Department of Health, Alexander Fleming House, Elephant and Castle, London SEI 6BY, UK E. GEISSLER, Akademie der Wissenschaften der DDR, Zentralinstitut fur Molekularbiologie, Lindenberger Weg 70, DDR-1115, Berlin-Buch, DDR MARY-JANE GETHING, Imperial Cancer Research Fund, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK K.I. GIBSON, Department of Education and Science, Medical Research Council, 20 Park Crescent, London W.I., UK JACQUELINE GITS, Smith Kline-Rit S.A., 89 Rue de l'lnstitut, B-1330 Rixensart, Belgium W. GOEBEL, Institut fur Genetik und Mikrobiologie, Rontgenring 11, 87 Wurzburg, FRG DONNA HARBER, Association of Scientific Technical and Managerial Staffs, 10-26A Jamestown Road, London NWl 7DT, UK D.O. HAINES, Health & Safety Executive, 25 Chapel Street, London NWl 5DT, UK T.J.R. HARRIS, Animal Virus Research Institute, Pirbright, Woking, Surrey, U.K. M. HASHMI, United Nations Environment Programme, PO Box 30552, Nairobi, Kenya, East Africa L. HATCH, National Institute for Occupational Safety and Health, 4676 Columbia Parkway (C-17), Cincinnati, Ohio 45226, USA G. HOLT, Commission of the European Communities, 14 Adams Square, Crook Log, Bexleyheath, Kent DA6 8BX, UK T. UNO, Laboratory of Genetics, Faculty of Science, University of Tokyo, Hongo, Tokyo, Japan J. INGLE, Agricultural Research Council, 160 Great Portland Street, London WIN 6DT, UK I.S. JOHNSON, Eli Lilly & Company, Lilly Research Laboratories, 307 East McCarty Street, Indianapolis, Indiana 46206, USA L. KAARIAINEN, Department of Virology, University of Helsinki, Haartmaninkatu 3, 00290 Helsinki 29, Finland SIR J. KENDREW (COGENE), EMBL, Postfach 10.2209, 6900 Heidelberg, FRG N.O. KIELDGAARD, Institute of Molecular Biology, Aarhus University, DK-8000 Aarhus C, Denmark χίν Participants Η.F. KLEFENZ, Schiller Strasse 10, D-6701 Hochdorf-AsSenheim 2, FRG M.A. KOCH, Robert-Koch Institut, Nordufer 20, D-1000 Berlin, FRG M. KOLOSOV, Institute of Bio-organic Chemistry, USSR Academy of Sciences, 117312 Moscow, Vavilov Street 32, USSR SIR HANS KORNBERG, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 IQW, UK E. LAING, Department of Botany, University of Ghana, Legon, Accra, Ghana B.W. LANGLEY, ICI Corporate Laboratory, PO Box 11, The Heath, Runcorn, Cheshire, UK A.F. LANGLYKKE (COGENE), Frederick Cancer Research Center, PO Box B, Frederick, Maryland 21701, USA ELEANOR LAWRENCE, Association of British Science Writers, Nature, 4 Little Essex Street, London WC2, UK R. LEWIN, Association of British Science Writers, New Scientist, Kings Reach Tower, Stamford Street, London SEI, UK H.W. LEWIS, Division of PCM Biology, National Science Foundation, Washington, DC 20550, USA B-C LIN, Institute of Botany, Academia Sinica, Taipei Taiwan A. LINDBERG, Swedish Natural Science Research Council, Statens Bakteriologiska Laboratorium, Pack,S-105 21 Stockholm, Sweden R.G. LLOYD, Genetics Department, University of Nottingham, Nottingham NG7 2RD, UK EBBA LUND, Department of Veterinary Virology and Immunology, Royal Veterinary & Agricultural University of Copenhagen, 13 Bulowsvej, DK-1870, Copenhagen, Denmark A.P. MACLENNAN, WHO Special Programme on Safety Measures in Microbiology, World Health Organisation, 1211 Geneva 27, Switzerland K. MARKOV, Institute of Molecular Biology, Sofia 1113, Acad. Bontschev Street, Block 6, Bulgaria M. MARTIN, Department of Health, Education and Welfare, Public Health Service, NIH, Bethesda, Maryland 20014, USA B.J. MIFLIN, Biochemistry Department, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, UK DIANE J. MITCHELL, Department of Plant Biology and Microbiology, Queen Mary College, University of London, Mile End Road, London El 4NS, UK J.H. MORRIS, Medical Research Council, 20 Park Crescent, London WIN 4AL, UK Participants xv Κ. MURRAY, Department of Molecular Biology, Edinburgh University, Mayfield Road, Edinburgh, Scotland, UK V.R, OVIATT, WHO Special Programme on Safety Measures in Microbiology, World Health Organisation, Geneva, Switzerland H. PANNEKOEK, Biochemical Laboratory, State University, Leiden, Wassenaarseweg 64, Netherlands W.J. PEACOCK, CSIRO Division of Plant Industry, PO Box 1600, Canberra City, ACT 2601, Australia G.B. PETERSEN, Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand V. PIRROTTA, EMBL, Postfach 102209, Heidelberg, FRG R.H. PRITCHARD, Department of Genetics, University of Leicester, Adrian Building, University Road, Leicester LEI 7RH, UK O.W. PROZESKY, Foreign Relations Division CSIR Pretoria, National Institute for Virology, Private Bag X4, Sandringham 2131, Republic of South Africa H.A. RAUE, Department of Biochemistry, Free University, De Boelelaan 1085, Amsterdam, Netherlands M. RICHARDS, Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey RH3 7AJ, UK M.H. RICHMOND, Department of Bacteriology, university of Bristol, university Walk, Bristol BS9 4AN, UK R. RILEY (COGENE), Director, Agricultural Research Council, 160 Great Portland Street, London WIN 6DT, UK GILLIAN RITCHIE, Elsevier Publishing Company, Amsterdam, Netherlands F.R. ROLLESTON, Medical Research Council, Ottawa, Ontario KlA 0W9, Canada W.C. RUSSELL, Division of Virology, National Institute for Medical Research, Mill Hill, London NW7 lAA, UK W.J. RÜTTER, Department of Biochemistry & Biophysics, University of California School of Medicine, San Francisco, California 94143, USA J. SAMBROOK, Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York 11724, USA G.F. SAUNDERS, University of Texas System Cancer Center, M.D. Anderson Hospital, Department of Biochemistry, 6723 Bertner Avenue, Houston, Texas 77030, USA J.S. SCHELL (COGENE), Max-Planck Institute fur Zuchtingsforschung, Egelspfad, D-5000 Köln 30, FRG Participants JANE Κ. SETLOW, Biology Department, Brookhaven National Laboratory, Upton Long Island, New York 11973, USA V. SGARAMELLA (COGENE), Consiglio Nazionale delle Ricerche. Lab. CNR Genética, Biochimica Evoluzionistica, Via S. Spifanio 14, 27100 Pavia, Italy G. SHARPE, ICI Corporate Laboratory, PO Box 11, The Heath, Runcorn, Cheshire WA7 4QE, UK G. SIEWERT, Schering AG, Mikrobiologische Biochemie, Postfach 65 03 11, D-IOOO Berlin 65, FRG MAXINE SINGER, Building 37, Room 4A-01, National Institutes of Health, Bethesda, Maryland 20014, USA ANNA MARIE SKALKA (COGENE), The Roche Institute of Molecular Biology, Department of Cell Biology, Nutley, New Jersey 07110, USA K.G. SKRYABIN, USSR Academy of Sciences, Moscow Β 312, Vavilov Street 32, USSR D.A. SOUTHGATE (COGENE), Food Research Institute, Colney Lane, Norwich NR4 7UA, Norfolk, UK E. SPROTT, Unilever Limited, URL Colworth, Sharnbrook, Bedford, Beds. MK44 ILQ, UK P. STARLINGER, Institut fur Genetik, Weyertal 121, 5 Köln 41, FRG H.D. STETTEN JR. AND MARJORIE STETTEN, Building 1, Room 122, National Institutes of Health, Bethesda, Maryland 20014, USA M.G.P. STOKER (COGENE, Foreign Secretary Royal Society), Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London WC2A 3PX, UK J. SUBAK-SHARPE, Institute of Virology, University of Glasgow, Church Street, Glasgow Gil 5JR, UK Y. SVERDLOV, Institute of Bio-organic Chemistry, USSR Academy of Sciences, 117312 Moscow, Vavilov Street 32, USSR W, SZYBALSKI, McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706, USA A. TALAVERA, Consejo Superior de Investigaciones Científicas, Instituto Biología del Desarrollo, Centro de Biología Molecular, Universidad Autónoma de Madrid, Canto Blanco, Madrid 34, Spain S-T TAN, Kumpulan Guthrie SDN.BHD Malaysia, Chemara Research Station, Jalan Labu, Seremban, Negri Sembilan, Malaysia V.l. TANYASHIN, Institute of Biochemistry & Physiology of Microorganisms, USSR Academy of Sciences, Pushchino on Oka, Moscow 142292, USSR J.R. TATA, National Institute for Medical Research, Mill Hill, London NW7 lAA, UK Participants xvii NATALIE M. TEICH, Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London WC2A 3PX, UK R. THOMAS, Academie Royale de Belgique, 2 Rue du Plagniau, B-1330 Rixensart, Belgium CONGRESSMAN R. THORNTON, PO Box 521, Sheridan, Arkansas 72150, USA G. THRELFALL, Searle Research Laboratories, Lane End Road, High Wycombe, Bucks., UK C.F. THURSTON, Microbiology Department, Queen Elizabeth College, Campden Hill Road, London W8 7AH, UK J. TOOZE (COGENE), EMBO, Postfach 1022.40, Heidelberg 69, FRG A. VAN KÄMMEN, Royal Dutch Academy of Sciences, Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, Netherlands M.C. VAN MONTAGU, Rijksuniversiteit Gent, Lab. Histologie en Genetika, Ledeganckstraat 35, B-9000 Gent, Belgium J.K. VASS, Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, Scotland, UK P. VENETIANER (COGENE), Biol. Research Centre, Hungarian Academy of Sciences, 6701 PO Box 521, Szeged, Hungary M.V. VIOLA, The Medical School, University of Connecticut Health Centre, Room L. 2025, Farmington, Connecticut 06032, USA L.P. VISENTIN, Division of Biological Science, N.R.C. Canada, 100 Sussex Drive, Ottawa, Canada KiA 0R6 R. WALLGATE, Association of British Science Writers, Nature, 4 Little Essex Street, London WC2, UK SIR F. WARNER, 140 Buckingham Palace Road, London SWl, UK I. WATANABE (COGENE), Department of Molecular Biology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan J.D. WATSON, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA C. WEINER, Program in Science Technology & Society, Massachusetts Institute of Technology 20B-231, Cambridge, Mass. 02139, USA D.L. WEISS, National Research Council, National Academy of Sciences, 2101 Constitution Avenue, N.W. Washington, DC 20418, USA EDITH BROWN WEISS, American Society of International Law, Georgetown University Law Center, 600 New Jersey Avenue, N.W. Washington, DC 20001, USA R.A. WEISS, Imperial Cancer Research Fund Laboratories, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
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