Reaching New Heights Antioxidants in Wheat

54 Pages·2013·4 MB·English

Reaching New Heights
Antioxidants in Wheat
Ronald Madl, Ph.D
Research Professor
Director Emeritus, Bioprocessing and
Industrial Value Added Program
Department of Grain Science & Industry
Food and
Agriculture
Lecture Series
July 18, 2013
Perth, Australia
Trends
Fiber Content


Metabolic Changes


Antioxidant Activity


Prebiotic properties

Oxidation processes are responsible for:

- Chronic inflammatory disease
- Coronary artery disease
- Rheumatoid arthritis
- Alzheimer’s disease
- Cataracts
Free radicals of oxygen are primary mechanism

Beta Carotene

Vitamin E

Vitamin C

Phytochemicals

- Chelating agents (Phytate)
- Estrogens
- Isoflavones
- Lignans
- Phenolic Acids
Caffeic acid
HO
COOH

HO
Chlorogenic
HOOC OH
acid OH
O
HO O OH

OH

COOH
Ferulic acid
HO
OCH
3
trans - Ferulic Acid
Decker, et al, 2002, CFW, 47, 8, 370
History: In 1990s medical literature reported

that wheat bran suppressed cancer cell growth
in vitro. Later results conflicted.
1994 mouse study showed red wheat bran had

protective effect against chemically induced
colon cancer vs white wheat bran with equal
fiber content.
KSU scientists undertook a survey of over 90

wheat cultivars, representing 5 classes to
determine variation in antioxidant activity.
Screen wheat- Develop in vitro assays -
representing 5 classes and 34 cultivars by:
Ortho-phenolic (PheOH ortho) concentration.
How much of these antioxidants do they contain?

Cell culture assay (IC ).
50
How good are they at killing colon cancer cells?

Anti-tumor activity: In vivo min Mouse study
PheOH Concentration
ortho
Values are given in apparent
µg (caffeic acid equivalents) per gram of dry grain meal.

High values are better.

Do grains contain different proportions of ortho
phenolics?

YES!