LIST OF ABBREVIATIONS Abbreviation Meaning D.W. Distilled water DNA Deoxyribonucleic acid dNTPs Deoxyribonucleotide triphosphate EDTA Ethylene diamine tetra acetic acid EtBr Ethidium bromide IPTG Isopropyl – β- D- thiogalactoside LB Luria- Bretani LMP Low Melting Point PBS Phosphate buffer saline PCR Polymerase chain Reaction PK Buffer Proteinase K Buffer PK Enzyme Proteinase K Enzyme R.T. Room temperature RE Restriction enzyme RoTat Rode Trypanozoon Antigen Type RNase Ribonuclease RPM Revolution per minute SDS Sodium dodecyl sulphate SOC Super Optimal Broath with Catabolite Repression TAE Tris- Acetate EDTA TbAT1 Trypanosoma brucei adenosine transporter 1 TevAT1 Trypanosoma evansi adenosine transporter 1 TE Tris EDTA UV Ultra Violet VSG Variable Surface Glycoprotein X- gal 5- bromo-4-chloro- 3 –indolylβDgalatase Units of Measurement % Percentage µg Microgram µl Microlitre 0C Degree Celcius A Absorbance bp Base pair cm Centimeter Da Dalton g Gram (s) h Hour (s) IU International unit Kbp Kilo base pair KDa Kilo Dalton Kg Kilogram M Molar ma Milli ampere mg Milli gram min Minute (s) Min Minute ml Milli litre mm Mili meter mM Milli molar ng Nano gram OD Optical density pmol Picomole sec Second (s) U Unit V Volt V / cm Volt per centimeter V / V Volume / volume W / V Weight / volume Identification and Molecular Characterization of Variable Surface Glycoprotein and Adenosine Transporter 1 Genes of Trypanosoma evansi of Camel Origin Å¡V ls i`Fkd VªhiSukslksek bokUlh ds osjh;scy ljQsl XykbdksizksVhu vkSj ,Mhuksflu VªkaliksVZj 1 thu dh igpku vkSj vkfUod vfHky{k.k REHAB ALI IBRAHIM YAGI THESIS DOCTOR OF PHILOSOPHY (Veterinary Parasitology) 2013 Department of Veterinary Parasitology College of Veterinary and Animal Science Rajasthan University of Veterinary and Animal Sciences, Bikaner – 334 001 (Rajasthan) Identification and Molecular Characterization of Variable Surface Glycoprotein and Adenosine Transporter 1 Genes of Trypanosoma evansi of Camel Origin Å¡V ls i`Fkd VªhiSukslksek bokUlh ds osjh;scy ljQsl XykbdksizksVhu vkSj ,Mhuksflu VªkaliksVZj 1 thu dh igpku vkSj vkfUod vfHky{k.k THESIS Submitted to the Rajasthan University of Veterinary and Animal Sciences, Bikaner In partial fulfilment of the requirements for the degree of DOCTOR OF PHILOSOPHY (Veterinary Parasitology) By REHAB ALI IBRAHIM YAGI 2013 Ph.D (Veterinary Parasitology) THESIS 2013 YAGI, R.A.I Rajasthan University of Veterinary and Animal Sciences, Bikaner College of Veterinary and Animal Science, Bikaner CERTIFICATE - I Date: …………….. This is to certify that Rehab Ali Ibrahim Yagi has successfully completed the PRELIMINARY EXAMINATION held on 11- 06-2012 as required under the regulations for the degree of DOCTOR OF PHILOSOPHY. ( ) Dr. G. S. Manohar 61 Head, Department of Veterinary Parasitology, College of Veterinary and Animal Science, Bikaner. 62
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