Radiation of Several Carabina Groups (Coleoptera, Carabidae) Inferred from the Mitochondrial ND5 Gene Sequences

5 Pages·1996·0.88 MB·English

by  Su, Z-H

E、l,tna,To;k、,o,24(2):175-l79,November15、1996
RadiationofSeveralCarabinaGroups(Coleoptera,Carabidae)
Inferred fromtheMitochondrialND5GeneSequences
Zhi-HuiSU,TokindoS. 0KADA,SyozoOsAwA
BiohistoryResearchHali,l-l Murasaki-oho,Takatsuki,Osaka,569-llJapan
and
Brun0DAVID,Jean-LouisDOMMERGUES,Fran oiseMAGNIEZ
CentredeSciencesdelaTerre,6,Bd.GabrielF-21000,Dijon,France
Abstract TheDNAsequencesofthemitochondrialND5geneweredetermined
for9FrenchCarabina-species.Themolecularphylogenetictreeswereconstructed from
thesesequences inconjunction withthose from therepresentativeJapaneseCarabina-
species.Theresultssuggestthatarapidradiationofthemajorgeneraexaminedtookplace
intheinitialstageoftheCarabinaevolution.
Keywords: ND5;mtDNA;phylogeny; radiation; FrenchandJapaneseCarabina
beetles.
OneofthepapersbySuetal.(1996a),inwhichaphylogenetictreeoftheJapa-
neseCarabinaewasconstructedbasedon themitochondrialDN5(NADHdehydroge-
nasesubunit5)genesequences,showedthat theJapaneseCarabinamaybeclassified
into5maingroups,mostofwhichcorrespondtothegenera[oftentreatedasthesub-
generaofCalabus(s lat)] that arerecognized in thecurrent classificationsystem
basedonmorphological characters(seefig 5 ofSuet al., 1996a). ThesearePro-
crustes (Megodontus=Pachyc,-anion), Damaste, (Damaste'十Acoptolabrus十Copto-
1abrus), Apotomopte,-us (L加1noca'-abus十Euleptoca'a' btls), Leptocarabus (Lepto-
catabus十Ade1oca,abus十Atl1onoca,abus十Pentacatabus十Ast11enoca,abus),Hemlca,・abus
(ffemica,abus十Homoeoca,,abus), andCa,-abus(Ca,・abus十0homopterus). It wasalso
noted thatCa,-abus (s. str)andOhomopte,・usmaybetreatedasseparatedgroupsbe-
causeofthebootstrapconfidencevalueoftheclusterrepresentingthesetwogenerais
Abbreviations:ND5,NADHdehydrogenasesubunit5,mt,mitochondria;bp,basepair;UPGMA,un-
weightedpair-groupmethodwitharithmetricmean;NJ,neighbor-joining;Myr,millionyears.
ThenucleotidesequencedatareportedinthispaperwillappearintheDDBJ,EMBL,andGenBanknu-
cleotidesequencedatabaseswiththeaccessionnumbersD86203-D86211.
Correspondenceto:S. 0sAwA.
76 Zhi-HuiSite1 1
88 Oamaster -1ρenn1s ]
62 Damaster b/aptoides
Damaster
Acopto/aOrus go 'n,,
Copto/abrus fruhstorferl
43 79 Megodontus o/0e1 ]
一一:、
Megodontus lo/aceus Procrustes
Procrustes cor1aceus
Chrysocarabus auronlte/1s Chrysocarabus
-
Arc 1caraOus nemora/1s - Archicarabus
100 「 一 Eu/eptocarabus porrectlco//Is -l
1mnocaraOus maack1 」 Apotomopterus
Pentacarabus harmandt Pentacarabus
-
Asthenocarabus opacu/us Asthenocarabus
-
ChaetocaraOus Int'1catus Chaetocarabus
_
Homoeocarabus maeander ]
Hemicarabus
1-lemicarabus tubercu/osus
Oreocarabus g/abratus Oreocarabus
L_l Mesocarabus prob/ematlcus 一 Mesocarabus
Autocarabus a,uratus Autocarabus
100 chemoρtorus lnsu1/co/a ] Ohomopterus
l 0畯用opteru aoon, - s
Carabus van、/olxem1
]
99 CaraOus conciliator Carabus
Carabus arve/1s;s
Leptocarabus proceru/us ]
Leptocarabus
79 Au/onocarabus kurilensts
Distance
0.06 0.05 0.04 0.03 0.02 0.01 0
Fig. Ia
notveryhigh.Thetreesuggeststhattheabove6(or7)maingroupsradiatedmorethan
10Myragowithinashortperiod fromtheircommonancestor.
Inthepresentpaper,9FrenchCarabina-species, thegeneraof7ofwhichdonot
occur inJapan, havebeenexamined forND5genesequences toobtain further evi-
denceforradiationofthemaingroups(=genera)oftheCarabinaandtoknowthephy-
logeneticrelationshipsbetweentheJapaneseandFrenchspecies.
Materials and Methods
TheFrenchspecimensusedwere collectedinBurgundy,andanalyzed for the
1069bp. upstream from 3'-terminal stopcodonof themitochondrialND5genese-
quences.For thepreparationofDNA,PCRdirectsequencing,andconstructionofthe
phylogenetictrees,seeSUetal. (1996a)andKUMARelal.(1993).Thephylogenetic
treeswereconstructedbytheUPGMA(KUMAReta1.,1993),andtheneighbor-joining
RadiationoftheCarabina 177
99 Oamaste rug'pennis
]
100 Oamaster 0/apto,dos
Aceρto/aOrrus geh1n11 Damaste
69 一 opto/abrus fruhstorfer1
Megodontus kolbe1 ]
Megodontus v/o/aceus P「oCustes
s cor1aceus 一
L ocarabus auron/tens ChrySocarabuS
]
l - cuteeptocarabus porrectlco//,s
100 1mnocafaOus maac ｽ APot0mopterus
rob'caraOus nemora/'s - Archicarabus
Mesocarabus prob/ematlcus - Mesocarabus
Autocarabus auratus Autocarabus
Oreocarabus g/abratus Oreocarabus
tacarabus harmandl Pentacarabus
thenocarabus opacu/us - Asthenocarabus
- Leptocarabus proceru/us -l
Leptocarabus
Au/onocarabus kurl/ensls 」
一
etocaraOus Int,,catus Chaetocarabus
Hem1lcaraOus tuoe,cu/osus ]
99 トHomoeocarabus maeander Hemicarabus
100 0homopterus lnsu/lco/a ]
l Oohboimopterus yaconlnus Ohomopterus
bus vanvolxem1
]
99 Carabus corlc//later Carabus
100 CafaOus arvensls
0.02 D
_
Fig. lb
Fig.1. PhylogenetictreesofthemitochondrialND5geneoftheFrenchandJapaneseCarabina-species.
a:UPGMA-tree b:NJ-tree.Thebootstrapconfidencelevel(basedon1,000resamplings)ofmorethan
40% isshownateachbranchingpoint.D indicatesKIMURAstwo-parameterevolutionarydistance
(KIMURA,1980).
(NJ) method (SAIToU& NEI, 1987), using theevolutionary distancescomputedby
KIMURA'stwo-parametermethod(KIMURA, 1980).Thetreeswereevaluatedusing the
bootstraptest(FELsENsTEIN,1985).Fortheseanalyses,theND5genesequencesfrom9
Frenchand17 representativeJapaneseCarabina-species(seeSuetal.,1996a)were
used.
Results and Discussion
ThroughouttheND5genesequencesused, neitherdeletionsnor insertionswere
required formultiplealignmentandtheG十Ccontentswerenearly constant (21.0±
1.0%).Themaximumdifferencepercent inallsites,and thatatcodonthirdpositions
intheND5genebetweenallthespeciesexaminedwerel4%and37%, respectively.
179 Zhi-HuiSしeta1
MultiplesubstitutionswerecorrectedbyK」MURA'sformula(KIMURA,1980).This,to-
getherwithverylittledifferenceinG十CcontentofthegenethroughouttheCarabina-
species,suggeststhelackofappreciableeffectsofbasecompositionbiasandmultiple
substitutionson thephylogenetic trees.Partial discrepanciesbetween theUPGMA-
andNJ-treeswere found forsomebranchingordersrepresentedby nodeswithlow
bootstrapvalues.Theseinstabilitydidnotaffectthemainconclusionsinthispaper.
F量i eonlineageswererecognizedinthetrees(Fig.1),i.e.,0homopterus,Car-abus
(s.str),Alc11icata' bus,Apotomopterus (s. lat), Hemicarabus(s lat),Ast;1enocarabus,
F)e,ntaca,・abus, Leptocalabus (s. lat), Mesoca,-abus, Autocarabus, 0,coca,-abus,
Damaste''(s lat),Pt'ocrustes十Megodontus, C11rvsoca1-abus,andCllaetoca,-abus.The
branchingorderofthese15groupswasnotconfidentlyresolveli,becausetheirbranch
length(bootstrapvalueoflessthan70)isnotsignificantlydifferent fromzeroinmost
cases. Ca,abus十Otic,noptet-us, Ast;1enocarabus十P,entaca,-abus, and Damaster十
Megodontus十Pl,oc1-ttstesmight respectively formonecluster.Evenif it isso, their
branchingpointsaredeep. Theresultsinturnsuggest that the15groupsmentioned
aboveemergedwithinashortperiod, i.e.,theyradiatedrapidlyintheinitialstageof
theCarabinaevolution.
Datingoftheradiationisdifficult.Takingthebasesubstitutionrateestimatedfor
several arthropods'mitochondrial DNA(1.2X10-8/silentsite/year; BRowER, 1994),
theCarabinaradiationmaybeestimatedtohavetakenplaceca llMyrago.This
wouldbetheminimumestimationasdiscussedbySuetal.(1996a,b). Ifwesuppose
thatthediversificationofDa,11aste,・(s.str)wouldhavestarteduponseparationofthe
JapaneseArchipelago from theEurasianContinent, whichwasestimated tohaveoc-
curredabout15Myrago,thentheradiationwouldhaveoccurredca 35Myrago(see
SUetal.,1996b).
SixFrench(European)genera,Atchica,abus(,feme,alls),Chaetocarabus(lntrica-
tus),Atitoca'a' bus(atiratus),Mesoca'abus(p''oblematlcus), 0reocarablis(glab,atus),
andChrvsoca'a' btis(atao' '1ltens)whichareabsent inJapan,donotshowcleardirect
phylogenetic relations toany of theJapanese Carabina genera. Two Megodotltus-
species,kolbel(Hokkaido,Japan)andvlolaceus(France) formacladeasexpected.C
conc'/faro''issometimestreatedasasubspeciesofCa,-vensfs. Indee C conciliate・,
(Hokkaido,Japan)isclosetoCan,ensls(France)ontheND5phylogenetictrees.Sup-
pose that these twospeciesbranchedoff from their commonancestor somewherein
theEurasianContinentandreachedFranceandJapan,respectively.Thebranchlength
from thenode thatsupportsthese twospecies roughly representsthe relative time
elapsedsincetheysplit. IfweassumetheCarabinaradiationtohaveoccurred35Myr
ago(seeabove), then thebranch length of the two Ca,abus-speciescorresponds to
15Myr.Inotherwords,ca.15Myrwererequired for therespectivespeciestohavear-
rivedatthepresentlocalitiesafter theirseparation. Thishasalsotoberelatedtothe
timeofseparationoftheJapaneseArchipelagofromtheEurasianContinent,andmay
strengthentheideathat theinitialCarabinaradiationisolder thangivenbyBRowERs'
substitutionrate.
RadiationoftheCarabina 179
要 約
蘇 智意, 岡田節人, 大澤省三, B.DAVID,J.-L.DoMMERGUEs,F.MAGNIEz:ミトコンドリアND5
1;堂伝子のDNA塩基配列に基づくォサムシ類の放散. - フランス産オサムシ1flitt・の9属 (7属
は日本にぃなぃ) 9種について, ミトコンドリアND5造伝-f-のDNA塩基配列を決定し, 日本の
代表的オサムシの配列とともに分子系統樹を作成した. 分類学上の属 (ときにilf属とされる)
に相当するl5の系統の存在が示されたが, これらは, オサムシ亜族の分化初期の短期間に放散
したと推定される.
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Elyt・ta,「okyo,24(2):179-l80,November15,1996
AdditionalRecordsofEu:clestoclsbicornutus(Coleoptera,Ciidae)
Makoto KAWANABE
BioindicatorCo.,Ltd.,Takada3-16-4,Toshima-ku,Tokyo,171Japan
Euxestocis bicornutus MIYATAKE, 1954 [Japanese name: Futatsunotsuya-tsutsukinoko-
mushi],isrelativelyrareamongtheJapaneseolidspecies,andithasbeenconsideredtobeen-
demictoShikoku(MIYATAKE, l985).Thisolidisrecentlycollectedfromratherwideareasin
Japan,andKITABATA(l993)recordedit fromcentralHonshu. Inthisreport, Iwillsummarize