Protein Phosphorylation in Control Mechanisms

307 Pages·1973·5.807 MB·English

by  F. Huijing (Eds.)

minmi uimTER svmposm
1. W. J. Whelan and J. Schultz, editors·. HOMOLOGIES IN ENZYMES AND META-
BOLIC PATHWAYS and METABOLIC ALTERATIONS IN CANCER,* 1970
2. D. W. Ribbons, J. F. Woessner, Jr., and J. Schultz, editors·. NUCLEIC ACID-PROTEIN
INTERACTIONS and NUCLEIC ACID SYNTHESIS IN VIRAL INFECTION,* 1971
3. J. F. Woessner, Jr. and F. Huijing, editors: THE MOLECULAR BASIS OF BIOLOGICAL
TRANSPORT, 1972
4. J. Schultz and B. F. Cameron, editors: THE MOLECULAR BASIS OF ELECTRON
TRANSPORT, 1972
5. F. Huijing and E. Y. C. Lee, editors·. PROTEIN PHOSPHORYLATION IN CON-
TROL MECHANISMS, 1973
ό. J. Schultz and H. G. Gratzner, editors·. THE ROLE OF CYCLIC NUCLEOTIDES
IN CARCINOGENESIS, 1973
*Published by North-Holland Publishing Company, Amsterdam, The Netherlands.
minim WHITER svmposm-uoiumE 5
Protein
Phosphorylotion
in Control
mechanisms
edited by
F. Huijing
E.V. C. lee
DEPARTMENT OF BIOCHEMISTRY
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
MIAMI, FLORIDA
Proceedings of the Miami Winter Symposia, January 15-16, 1973,
organized by the Department of Biochemistry, University of Miami
School of Medicine, Miami, Florida
®
ACADEMIC PRESS New York and London 1973
A Subsidiary of Harcourt Brace Jovanovich, Publishers
COPYRIGHT © 1973, BY ACADEMIC PRESS, INC.
ALL RIGHTS RESERVED.
NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR
TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC
OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY
INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT
PERMISSION IN WRITING FROM THE PUBLISHER.
ACADEMIC PRESS, INC.
Ill Fifth Avenue, New York, New York 10003
United Kingdom Edition published by
ACADEMIC PRESS, INC. (LONDON) LTD.
24/28 Oval Road, London NW1
Library of Congress Cataloging in Publication Data
Main entry under title.
Protein phosphorylation in control mechanisms.
(Miami winter symposia, v. 5)
"Proceedings of the Miami winter symposia, January 15-
16, 1973, organized by the Dept. of Biochemistry,
University of Miami School of Medicine, Miami, Florida."
1. Phosphorylation-Congresses. 2. Proteins-
Congresses. 3. Cellular control mechanisms-
Congresses. 4. Cyclic adenylic acid-Congresses.
I. Huijing, F., ed. II. Lee, Ernest Yee Chung, ed.
III. Miami, University of, Coral Gables, Fla. Dept. of
Biochemistry. IV. Series. [DNLM: 1. Phos-
phorylase kinase—Congresses. 2. Phosphotransferases—
Congresses. W3 M1202 v. 5 1973. XNLM: [QU 135 P967
1973]]
QP535.P1P76 1973 599'.01'9214 72-9329
ISBN0-12-360950-X
PRINTED IN THE UNITED STATES OF AMERICA
SPEAKERS, CHAIRMEN,
AND DISCUSSANTS
C. Abell, Department of Human Biological Chemistry and Genetics, Univer-
sity of Texas, Galveston, Texas
V. G. Allfrey, Department of Cell Biology, Rockefeller University, New York
S. H. Appel, Division of Neurology, Duke Hospital, Durham, North Carolina
/. Ashmore, Department of Pharmacology, Indiana University School of
Medicine, Indianapolis, Indiana
S. A. Assaf, Department of Medicine, University of Miami School of
Medicine, Miami, Florida
M. Bitensky, Yale University, New Haven, Connecticut
A. Braunwalder, Department of Microbiology, CIBA Geigy Corp., Summit,
New Jersey
B. L. Brown, Institute of Nuclear Medicine, London, England
R. Chalkley, Department of Biochemistry, University of Iowa, Iowa City,
Iowa
B. S. Cooperman, Chemistry Department, University of Pennsylvania, Phila-
delphia, Pennsylvania
/. D. Corbin, Department of Physiology, Vanderbilt University, N.ashville,
Tennessee
C. Dalton, Hoffman-La Roche Inc., Biochemical Pharmacology, Nutley,
New Jersey
G. H. Dixon, (Session Chairman), Department of Biochemistry, University
of Sussex, Salmer Brighton, England
/. Fessenden-Raden, Department of Biochemistry, Cornell University,
Ithaca, New York
ix
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
P. A. Galand, Nuclear Medicine, Free University of Brussels, Brussels,
Belgium
G. N. Gill, Department of Medicine, University of California, San Diego,
California
A. G. Gornall, Department of Clinical Biochemistry, University of Toronto,
Toronto, Ontario, Canada
P. Greengard, (Session Chairman), Department of Pharmacology, Yale
University Medical School, New Haven, Connecticut
L. R. Gurley, Los Alamos Scientific Laboratory, University of California,
Los Alamos, California
B. L. Horecker, (Session Chairman), Department of Physiological Chemistry,
Albert Einstein College of Medicine, New York
F. Huijing, Department of Biochemistry, University of Miami School of
Medicine, Miami, Florida
E. M. Johnson, Rockefeller University, New York
G. A. Kimmich, Department Radiological Biology and Biophysics, University
of Rochester, New York
E. G. Krebs, Department of Biological Chemistry, University of California,
Davis, California
G. Krishna, National Institute of Health, Bethesda, Maryland
T. A. Langan, Department of Pharmacology, University of Colorado Medical
School, Denver, Colorado
G. 5. Levey, Department of Medicine, University of Miami School of
Medicine, Miami, Florida
R. W. Longton, Naval Medical Research Institute, National Naval Medical
Center, Bethesda, Maryland
H. R. Mahler, Department of Chemistry, Indiana University, Bloomington,
Indiana
J. M. Marsh, Department of Biochemistry, University of Miami School of
Medicine, Miami, Florida
x
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
O. J. Martelo, Department of Medicine, University of Miami School of
Medicine, Miami, Florida
K. S. McCarty, Department of Biochemistry, Duke Medical Center, Durham,
North Carolina
/. P. Miller, ICN Nucleic Acid Research Institute, Irvine, California
C. Moore, Albert Einstein College of Medicine, New York
C. R. Park, Department of Physiology, Vanderbilt University, Nashville,
Tennessee
R. Piras, Instituto de Investigaciones, Bioquimicas Fundacion Campomar,
Buenos Aires, Argentina
L. J. Reed, Clayton Foundation, University of Texas, Austin, Texas
E. Reimann, Department of Biochemistry, Medical College of Ohio, Toledo,
Ohio
M. S. Rose, ICI Industrial Hygiene Research Laboratories, Cheshire, England
O. M. Rosen, Department of Medicine and Molecular Biology, Albert
Einstein College of Medicine, New York
H. Rosenkranz, Director of Biochemistry, Mason Research Institute,
Worcester, Massachusetts
/. Roth, Section of Biochemistry and Biophysics, University of Connecticut,
Storrs, Connecticut
K Segal, Biology Department, State University of New York, Buffalo, New
York
R. Sharma, Department of Biochemistry, Veterans Administration Hospital,
Memphis, Tennessee
K. Shelton, Department of Biochemistry Medical College of Virginia,
Richmond, Virginia
H. Sheppard, Hoffman-La Roche Inc., Biochemical Pharmacology, Nutley,
New Jersey
K. S. Sidhu, Endocrine Laboratory, University of Miami School of Medicine,
Miami, Florida
xi
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
T. Soderling, Department of Physiology, Vanderbilt University, Nashville,
Tennessee
H. K. Stanford, (Session Chairman), President of the University of Miami,
Coral Gables, Florida
D. Steinberg, Department of Medicine, University of California, San Diego,
California
S. Strada, Program in Pharmacology, University of Texas Medical School,
Houston, Texas
M. Tao, Department of Biological Chemistry, University of Illinois,
Chicago, Illinois
V. Tomasi, Department of Physiology, University of Ferrara, Ferrara, Italy
W. J. Whelan, Chairman, Department of Biochemistry, University of Miami
School of Medicine, Miami, Florida
W. D. Wicks, Department of Pharmacology, University of Colorado Medical
Center, Denver, Colorado
/. Willis, P-L Biochemicals, Inc., Milwaukee, Wisconsin
C. Zeilig, Vanderbilt University, Nashville, Tennessee
xu
PREFACE
In January, 1969, the Department of Biochemistry of the University of
Miami School of Medicine and the University-affiliated Papanicolaou Cancer
Research Institute joined in presenting two symposia on biochemical topics.
These symposia have begun to develop as a tradition over the years and have
attracted national interest.
In 1970, the two symposia were entitled "Homologies in Enzymes and
Metabolic Pathways" and "Metabolic Alterations in Cancer." The full report
of this meeting was published as the first volume of a continuing series under
the title "Miami Winter Symposia."
In 1971, the value of the series was enhanced by including all discus-
sions as well as the full text of the reports. In 1972 it was decided to publish
the results of the two symposia separately, in order to prevent the volumes
from becoming unwieldy and to permit a wider scope in the selection of the
topics for the two symposia.
This volume, the fifth in the series, contains the proceedings of the
Department of Biochemistry's January 1973 symposia entitled "Protein
Phosphorylation in Control Mechanisms" and will appear simultaneously
with volume 6, the proceedings of the Papanicolaou Cancer Research Insti-
tute symposium on "The Role of Cyclic Nucleotides in Carcinogenesis."
The success of the Miami Winter Symposia is indicated by a continued
increase in the number of registrants, which this year reached 433. The
organizers may have to consider putting an upper limit on the number of
registrants in order to preserve the nature of the meeting.
An innovation this year was the presentation of short communications
on the free day between the two symposia. The abstracts of the communica-
tions related to protein phosphorylation are printed in this volume.
Associated with the symposia is a featured lecture of autobiographical
nature, named in honor of the Department of Biochemistry's distinguished
Visiting Professor, Professor Feodor Lynen. Past Feodor Lynen Lecturers are
Dr. George Wald, Dr. Arthur Kornberg, and Dr. Harland G. Wood. This
year's Lynen Lecturer was Dr. Earl W. Sutherland, who unfortunately fell ill
shortly before the symposia and was hospitalized in the University of Miami
Medical Center during the symposia. We are very grateful to Dr. C. R. Park,
longtime friend and colleague of Dr. Sutherland, who at short notice prepared
Xlll
PREFACE
and delivered a biographical lecture on behalf of Dr. Sutherland. This lecture
forms the opening paper of this volume.
The symposia are organized to ensure publication as rapidly as possible.
The editors want to thank all the contributors, the speakers for submitting
their manuscripts promptly, the discussants for editing their discussions, and
especially the secretarial staff.
We also acknowledge with gratitude the financial assistance of the
Howard Hughes Medical Institute, the Departments of Anesthesiology and
Dermatology of the University of Miami, Coulter Electronics, Inc., Eli Lilly
and Company, MC/B Manufacturing Chemists, Smith Kline and French, and
E. R. Squibb and Son.
In 1974 the topics of the Miami Winter Symposia will be "Carbo-
hydrates and Cell-Surface Recognition" and "Membrane Transportations in
Neoplasia." The Lynen Lecturer will be Dr. Luis F. Leloir. The symposia will
be held from January 14-18, 1974.
F. Huijing
E. Y. C. Lee
XIV
THE FOURTH FEODOR LYNEN LECTURE:
My Life and Cyclic AMP
Earl W. Sutherland
Professor of Physiology, Vanderbilt University
School of Medicine, Nashville, Tennessee
with a preface and editing by
Charles R. Park
Professor and Chairman
Department of Physiology
Vanderbilt University School of Medicine
Nashville, Tennessee
Dr. Earl W. Sutherland was to give this talk but could
not do so because of sudden and serious illness. I had
misgivings when he asked me to speak for him, but my enthu-
siasm developed as I realized that here was an opportunity
to present the man and his work to you in an unusual way.
Earl Sutherland being very human, did not have his lecture
prepared two weeks ahead of time and consequently could not
give me a manuscript to read to you. This is perhaps for-
tunate. Sutherland is like his late neighbor, Harry Truman,
who learned during his brilliant campaign for the presidency
that he transmitted his character and special way of think-
ing much more effectively when he spoke without a text. To
be frank, Sutherland's talks are sometimes minor disasters,
but, when the occasion inspires him, his lectures are at-
tractive, effective and quite unique. A few months ago, he
gave an account of his scientific work on an occasion at
Vanderbilt which provided this inspiration. I have taken
the present presentation from a tape of that talk, editing
for clarity but trying to preserve and transmit the
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