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Prokaryotic expression and ligand binding characteristics of pheromone binding protein ASP1 in the Chinese honeybee (Apis cerana cerana) PDF

2013·5.2 MB·
by  ChenWeng
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Preview Prokaryotic expression and ligand binding characteristics of pheromone binding protein ASP1 in the Chinese honeybee (Apis cerana cerana)

mk San ect Sir, Ser 39.986 HOLE ss ate PERE RRA EA ASP] ORR RIA BRERA HED HT ARB, tears! sa seo “ASSIS AE oH tant BEL E AERE how wrens we SED AL RMD HT REN FL St UES IRo Hawt: BSR, SARA PR AUR cine meet, PAIN AIG Soest REE MGCL NO HRCI E AAR Osea LAIR AS I SEAAETANHO IMGiOWE, [URLERARLER eS. ETE “BA. RISA RMR ATAREI a AbD 1 Toa ree er ere rere ce eee ee eee weabhs W3EM eb ast tk BENGAL, Cer Pe REIT RRR OTT ALLE ERENT ANH ES NORE EER Be Foti Po ACE eR IRS ad ETRE tit Mh aM SU APRN ED Acar SEs Diels SANE Ae heLeS: Goee WR 8 SEE. ot em aeisyte LE Prokaryotic expression and ligand binding characteristies of pherontone binding protein ASP1 in the Chinese honeybee (Apis cerana cerana} WWENG Chor! ZAG Lin-Ya'. ZAG Lei’, 1U Yuedie, LUO Cher", LE tongiaog! "(1 allege uf Le Soren, Chi iinge Lanes “jane Poona hee abuse a Beto and Luezectin & Cunt. angrbes 310016, Cina; 2. eats of Ment and krviotetal Prats, Being Alene of Asin une se Koray Sk com, Bling LUGOWT, Ching 3. Calle Ani Selene, Zhen Trivty. Thro SID08R, China Abednue [ts] Ue nude ho sng Auli a ecu ASP, a pean hindi, poten CPI) , ia ve Shane bannghioe px weeue enae) with phovenone wee wher plant vats. [Moveade] fa coeds: to obtain tho wonmbina: poten {Asce-ASI}, wo eters eoummted the cluiug aud poke enmeaion vere of Ane-ASPL, which wer ype! ie the plinieml alin ie the ecombiogrt sre vith Wocheniosl ants wes pitied, the hinding cepa of Acer-ASH1 ah phe-ovens allo kr was oat a i rampeii b Mavesva: nig ln: MPS go aod 1 Ahnresemoe gob. [Rae] Sion a te Tigris toed ened amemger Wining capi AsipASPL ard wu able lo dew: dhe tale Uuossecace levy of FS by anes asa 505 avons dora, eval 4 hel walonvaie, a iene perineal Lae senza ‘sompote with 1M. causing 9% 31 soSnetion in th nescence wensg. ant K, U9 eval’ To emily aldol. autor ye pena, cn 5.5% se Line te relative Maer ee Sstonnite, and K,=98, 44 mal. Novels, Ae ASP id met Wir th eter kunt of phoromre al all cangp. qucea phevomanc. La adilion, re plank sola. ethyl aby. healuarblonsele, 3.4 staal Meneeyde, alternate wal ate, shud cay 1 Hind with ASH ia various degces, [Uotehiion} Ibe scsube indicate tak Acer ASPL ceils REEL PERE PREIS. ENS, OT LERNER AT Nas PRET EN PERM SMH oT RSA. SPATS WG ah OS, Poe ann Sem Nis Baa ir OER PROSTREET NP MGS RTY a wt remarkable seis wil que pennies bind lt aoe plat slate oy so tle Thi rpieg ink Acer ASI om probate a pomoin having comy'cx ghilageal rotion. im shit roping gure peer ei slerml 9 iy sexnndary freon ey wards: nates, hig Feenon Hguad {ya semana csrasas vlmnie Ce oteiiluh, mace meo. 2 ELL EER IS EMME Vor Move ot oh 19981. URLICIAL Ap achat VERA , 2b TALL es ea IER 2OHORA, SUS RE RAARA ‘odio lilo prin, PBB), HISD ELarbr thing poten, ORPRE PRS, BL A ay PR Bin, a Jusiault ond Meroe: OMS WGI APLAR, QM ls IEE Wie SERRE IEMA HHL LIE Peek Fob ABR 5 One ay 2013, A EAR PTS HE 4 i AL AM BSE Rosny 2H, ALS YH AIEEE nl eli PSE Danis PRAIRIES 3 ATL Bi Conteans aperial pms’), Bl ASL. ASPE A ASED (Dauty ob at, 1098), BEET IED BL ASPL SE QTEN 1 EB (Dang of al 19095, ‘BEEHEH ASN ATO Ee a WRK BTR ert ot HPA Apts rian comive Poin EH EAE Levon Faber HEV, FeAl bobbi. alts vO HE Ast 1980, LLB PACER Ls ACNE ML, oh al FRE AUR CL BETTER, SRA MT LEI RE TAMRON. MET REE RELL 2 NO. BRUNE eK, MCLE EM A. wren co Yate TENE i, BMRMRIE RE ELITE, APR BARA SATE STMT, 208}, ETI Ah feo SAUDE THLE, PHA, * HeHESB EB A Kong ota, 1S) HAR ETB, Vining ating proksute eapmesions tin A CRITE. Uw FETA AEA RSE SHER MEALS WO) 27 WAS RR OA. WES WALA INIMES LETRA, km set Al P aS eR FRE Acer ASP, JPR, Aco A8D1 FEHLA FEET. SHAT pra Sabet BNE WEARER CT ath, sebum Feet pe EEN ORGS, 2008) AUTON, SIE Sect ASL DEI A. JE TA YEH aR Heth Pea AT. HPA AT AES Te deh LAT 1 HBS Lei PL, Stalag Re WRU pa NE Fes Behan a TL, BSE ASATIR DHS BLL {DES ART SETRTE, Eateg DNA ROME. AL AEB AUASIAE Somll [Ai isd #40 LoKalta 2 Fe Us AC RANT bak LS TEAERERE FOAL GF As ON 1.2 BARRGARYH pLIN/Acer-ASHL OHNE SCRUM MANCTCAT Ae ASIL HARD pEBL- REI FEE AEE IR BBAE LA, CL Bao T A ina ie HNC eT AS AU) AERA ABP PE APSIAP SMW, STI ARIS Zit BIMKIGA De OM. MTA PCR ge TRAY SABA Roe, RT ee eee ANPL IMRIVIA TES GORERAPEE ae 127 DED) ue nase RAUL O RRO LIU oe DoBR TMAH ru tsetnoon maces AS Yaa oa 0A? ® PA ate keener ae Becect » PE am . oy AL MET Beret ” ATE tam ai » SIRT We tos » HE | ite aes © {UR Fi erie ® RFC RBI ante % ‘eRE He t ” fara PLA Me pl » eee ® een 3 HiT AEN adel ” te al . Yar Hae Py stereo. Maaet » SPR RT Cans ed 5 “pyr, tals % He eR Fe vt “ae hn Ih emer It Se AED eI SRI NEA: SMELT PRR 13 BA AM BANB SRE AKAM AH ae HELE ERED 1% G7. ORAS onl RAR fy NG rad AE ag, BC 220 w/in BLE SSAA SAE OH EER SUPRA SEAT foe PIA ABER 20M ol 2100 pal wR Aca IRIS HL P04 ra JB bth Pa Shar a TTR PARIS omaléT. a 9007180 YER IIE LL 5 on eon BI SEE 6 oni Sabo ie. NORE I ada HERE: a IO oo iy SDS. PIGH Ege AR atal AAA MTs 8 ther A S ok KBE: GISWAT RELL ARRON TEE EMMA in, MAUR ie ASH ATR A BEA AT P20 PE WM win, AIEEE Ry PAGE IDERSILLE eA BLAKE 14 RARE ASH WUD TAL? Se USE Cain ak LATO. Ate ARARIDEHLS mol/1 ape PRS HERES, HE NTA AG UPWEMCH, TALS ATER SURE ISR ELT. 20, 53, 100. 200. 300 SOO mol BSCR ART BEBE IU SDS-PAGE Haken RATA OU futet, SOMA CTT RAL AST Hr IOGEAR ES BAT SPL. TRAM a 7 TIE PAS. EAN SNCS ATF AB 1. BIRCH SECT ARE eh EA, sii EO EL ANP Ta USA be Yet HOE OLAET BALLET I LS gpa ASP BEANS ARER LANDS hapa PAIL RN ET, Robe ANP) ES NPS HACE Sle, RUT TEIW 5301 FT TESL 0.8 rn ASDA EHF. 9.8 pooe/T. BE APN HL LL HSH AECL 00 AMTSRERAA 281 om. BA LAURIE 10 nu: SRE AMU UM AHS 8 pos) 24 SEM LPN ALARM | ANU BUNA Fe. IBATTIRITA Ie SE DARA ER ADCS. HL Sorcha [BAREIS 2006). a (LA) ~ 0.) #88F J EL NON ee, TT RL LMP E87 ir) UA OPRELSESAA Or MEAN rear un LG ship ASP REMASTER See SCAR AER NPY 48 AHTEI ME ASEL IESE CARRE Pl ALB, GABBA Uriah, st FHL, PURI SGI TBS SCARE let ReaM lO SAR ASP RATS aE SADE. SRT HAR TLE aR EL aH FAA AMS APN AEA ASP RSS DPN EGTA W moo L Ae, 2 BRST JTC RHP ARSE, ATMS SO a AMES ER, ay hates Hl 26 ART TRA POL AE Roe Nin 2h ey AS HCPL. 2008). BIWKE, He Ke FIER RE TIE, SATB th HOE Ry das RARER SBA (TN, Teo BLAS. A I SE END FEAACTRES SOCAN A MEE CAL AU RSMAS 1B, MARE RED PARE. I-SPN, ARIES LMP PEEL. Bigg MEALSCHIER ASI HUGE AROS. FEM, SBR TF Ace ef ASV LAND CARER. BRET (RTRs 17 mle st SAURBE NOMA U1 Rr REP 2.08 e988) ay <4 J sense i" Jamas 2 Wd) Ak ate FHL ae A: RN RtaHLeBIUNeRE BD He ASA rpg) 3 eer 2 PA ASDL SOILED edesn orn soe p SMEs ptr MER al Mea hn? ee Ee SOL ea ds i han dT 22 shih ASP SERRA MIE, Fl? BAO), HY-BAL I-SHbaEOL MIS TARIG pLUS2/Aeor-ASPL I ETTEAL IRAE WT PEERS, SP UTR ASLAM, SLAM, AGUA. 1, SMCRALILSTALAT, MALE, RET SRSA | WB, PTA oo PORE ESP Ace ASPL BEA PRS GHBTET RIN, fk ASDA. JF SNM A WIG ER TMs Come L. HEI SA. SUSTACL Bil 2.3 PM ASPL RESET SER ASPI nme 2, AN. PTE ASP SAL NPN PONIES ENE CH SAU IL, NEA UIA SEA 2B) FLL 0.78 yvelL AEE Aree APL AK ETPHLGT-NPN RAGED 8 mel L ASHER NIA MEE, TEN MU oT SP IAPS, ATR ASER ORIEL. UNDNSGERIET HELA SOR ACS 16°58 1 NTN SPS PAC LAR AITO A HAA SEO, ol OETA, Hse R Fo dabban BapeRwacat ee pn 1 FIs RUHR Lav GRRL 7 Asease Erba ksi a ancl U8 sad yo ED i Some a SRT RKGUL LAPS ROR BOER CY eee TSE even se. Pn mt 6-H vine. el gr; Me ARNIS eerie Se 88 0 ACURA ste el pe ein So ASP ori 0.98, AH Sead SRR EASE 1H. RUE MUL, HASH RCO ARMCHAIR ASP B45 LY FREE Ae pL ER SR O07. AU, RA URAB Hh, MPS 'j AvceASH ZE TPR RAPE SC , 1-SON A ED Sven A8PL bP ubthinunA ae AS WRPUESNESS 4 S18 Sei S005 7 ATER LN (AH SARE 1008 FE SDS TFC ATA SCAR al PALI IAP HOR) WHER ASCHER TE INES IERE AIEEE. S410 30 LLIN Hat ABE, 298.4 nel ORE} 1 HERE CA ANI I ASUEIIE ETC TER SIT AA. GEA PARAS ASE Ce TERRY 3 Ne OSE Mal SE ASP Hse Ca SERET PUA OLASEH A PCR TET SE PSOE ERIS. PRD uaF AST AP NSA rath Baar ASPL RST By 2 MrROLETESEN ASP ISNA ATER EH 2E2. SPREE ULTOL-NEN Stace Rit ASI BERS eR vanes sai aa 2A THe a a LT AAEAWH Most Bde 2 Pak ng HP en see ferme BIS ERR CRETES. 208). TTR AE ee ee SPA TE Ash Ba, RN aEAUI MEY A RBISR LAF. MEAL RL erie: MEE, EAT WS, RRA Mah. TAT Rar Meme "ARE aoor A818 TRS HE A te Beef. INP USI A (PREECE APES cH Yer A OMY BEART ter: FS PR (Cin 213; THECFEM Stsbtee is HH SUPE (Lit sek, 2012) gush ASP2 (F121 SEA PALA, 20010, RET Meer ANP ARE {SUT INE Kon A, S.Lites SiR SE(R AL AY PP AIPA ASE CPE RAS AE. RIT SON LR mea Bh OA RY Fae Gi URI, GIR T bNR ASM peat 2HELERS ULE ey yc HOB) SUVA TIMI IEG, IU MON EE CMP Heer SSL ALL as RG, ASU. OM HAGLA, HST 1D ral, Bact 5 Aver ASPLALREIEAS 4 VA. SERAATULAT MOU, YS. pmol/L) TR AVA pL NOS PRE TENE. 5) EMR Sd grb a) EMTS FAURE, CL Aco SPI A185 5 IVA JOSE bE HOS EAE TEA pveres 7 in ay, ; sh. ic 2 Ru We Ge Kahr omer 1997) EAR ABEL TDS AV Oh, was HG PTAA IANS LP oY Ph SPELT IIE 1A ISIS EIB AL FETA: FE MISS Me, Coma, LT TG LAT AIT RR 1 RO BUG! O OTN ACR. SY AGS, FF 9AM APL ARTE BSE. ALBA ie a HE ASP WHEE SRA GA Meany £1000) AL me, HELA LDH Acer ASH FER OL SSE PE Aco ASHI B'S 3 ATE TAR SHR Lk MAE OWE ees NER FACE BLAS COMPS RIA, AAT ALD AnD ASHE CEES ULTRA APL eh ARLENE ab, Ha WME ERG AGE Kat AUR HS ASP REREAD RAs iso maea. E01 Hi Ochioag £22) A SH ATH LLG ETD pee 1.8 BG HP KL 1, WPMD STALEY eat 8. Roe TRAIN RITZ SEH Fae EMBASE PEAT: TAL Koseirg SPIDO13) AGES EH ETM lv ont hie nase AS he td ec ERA Sere pr te MALS Ht GHEE Uc Tbce RE CHEB RAS lt ASI Bs SERENE TERMITE, SE ALS APT RA LRN TE iE BEN (Reterences Loh Mee 38.280 ine: mines mtn ens on Hd, FEAT AT CAE, yin hie. AES EELHE Soo meesL AS LeeRT. SH Hii teh bee ths 6 tence (Din, Ae 2880 Fe he BER. 2M PRMESAC KE Sw aDH FRET ER Fy, S14 a [ee ne we! 21 KC, ang LF hg 5 ON. the 3. Say IE, 205 td, #20 ar Te pebRarnne yee sft Lie HY. le F, Teng Sb, 212, Raa pepe pheence bet Ise ah Fat andar bes, Ah atte dicen Bre. be, EB, Ue lave ye sine tn veoh RHR aM ML FERS! a1 Sng OF. HS, Bass 19K, By 8, 365, The etn TER HE fe 2H ANG Hcrhse Wea a8, 27. slat aT Tae 150 PEARL AP siren, Us

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