Proceedings of the 2006 Annual Multi-Crop Aflatoxin - ARS

125 Pages·2007·2.15 MB·English

Proceedings of the 2006 Annual
Multi-Crop Aflatoxin/Fumonisin
Elimination & Fungal
Genomics Workshops
Please join us in thanking the following industry organizations for their generous
support which made this workshop possible:

National Corn Growers Association Corn Refiners Association

Pioneer National Grain and Feed
Association

Monsanto North American Millers’
Associaton

PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
3
Table of Contents

Introduction …………………………………………………………………………………….12

Agenda …………………………………………………………………………………………19

Application of Multiple Approaches toward Reducing Aflatoxin Contamination of
Corn Grain
J.M. Headrick
…………………………………………………………………………………………..33

FUMONISIN ELIMINATION/FUNGAL GENOMICS, REGULATION OF AFLATOXIN
BIOSYNTHESIS

Moderator: Scott Averhoff, Texas Corn Producers Board

PLATFORM PRESENTATIONS

Biosynthetic Mechanism for Fumonisins, a Model System for Fungal Non-
Aromatic Polyketides
L. Du, X. Zhu, F. Yu, and K. Zaleta-Rivera
…………………………………………………………………………………………34

Use of RT-PCR to Detect Fusarium verticillioides during Endophytic
Colonization of Maize
D.F. Kendra and R. Dyer
…………………………………………………………………………………………35

Identification and Characterization of Regulators of FUM Gene Expression
R.A.E. Butchko, D.W. Brown, M. Busman, and R.H. Proctor
…………………………………………………………………………………………36

Microarray Analysis of Fusarium verticillioides
D.W. Brown, R.A.E. Butchko, D. Kendra, and R.H. Proctor
…………………………………………………………………………………………37

Sequence Analysis of Aspergillus flavus
G.A. Payne, J. Yu, M. Machida, D. Bhatnagar, T.E. Cleveland, W. Nierman, and
R.A. Dean
…………………………………………………………………………………………38

The Functional Genomic Basis of Antioxidant Potentiation of
Inhibition of Aflatoxin Biosynthesis
J.H. Kim, J. Yu, R.J. Molyneux, N. Mahoney, K.L. Chan, D. Bhatnagar, T.E.
Cleveland, W.C. Nierman, and B.C. Campbell
…………………………………………………………………………………………39

Aspergillus flavus Genomics for Solving Preharvest Aflatoxin Contamination in
Crops
J. Yu, J.R. Wilkinson, G.A. Payne, B.C. Campbell, D. Bhatnagar, T.E. Cleveland,
and W.C. Nierman
………………………………………………………………………………………...40
PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
4
Identifying Potential Aflatoxin Gene Regulators by Microarray Analysis
J.R. Wilkinson, J. Yu, J.M. Bland, H.K. Abbas, B.E. Scheffler, E. Mylroie, R.
Shivagi, W.E. Nierman, H.S. Kim, D. Bhatnagar, T. E. Cleveland, and W.P.
Williams
……………………………………………………………………………………….…..41

POSTER PRESENTATIONS

ESTs Provide Unique Insight to Fumonisin Regulatory Gene FUM21
D.W. Brown, R.A.E. Butchko, M. Busman, and R.H. Proctor
…………………………………………………………………………………………...42

SSR Fingerprinting Analysis of Vegetative Compatibility Groups in Aspergillus
flavus
C. Huang, R. Sweany, C. DeRobertis, and K. Damann
…………………………………………………………………………………….……..43

Identification and Characterization of Differentially Expressed Genes in Two
Inbred Maize Lines Using Microarray Technology
R.Y. Kelley, D.L. Boykin, S.M. Bridges T.D. Brooks, and W.P. Williams
…………………………………………………………………………………….…… 44

Fumonisin B1 Is Necessary for Corn Seedling Disease but is Minimally
Translocated from Roots to Shoots
A.M. Zimeri, L.D. Williams, R.T. Riley, and A.E. Glenn
………………………………………………………………………………….….……45

Corn Seedling Disease, Fusaric Acid as the Wilt Toxin and the Need for Biocontrol
of Fusarium verticillioides and other Fusarium species
C.W. Bacon, M.E. Snook, and D.M. Hinton
…………………………………………………………………………………..………46

PANEL DISCUSSION
Panel Chair: Jeffery Wilkinson ………………………………………………..…….47

The True Economic Impact of Aflatoxin in U.S. Crops
F. Wu
………………………………………………………….………………………………49

CROP RESISTANCE – GENETIC ENGINEERING

Moderator – Phil Wakelyn, National Cotton Council

PLATFORM PRESENTATIONS

Secondary Metabolite Biosynthetic Gene Clusters in Sequenced Aspergilli
W.C. Nierman, N. Fedorova, and J. Yu
…………………………………………………………………………………………50
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PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
5
Expression of an Active Form of Maize RIP 1 in Transgenic Peanut Inhibits Fungal
Infection and Reduces Aflatoxin Contamination
A. Weissinger, M. Wu, X. Wang, T. Isleib, T. Stalker, B. Shew, G. Payne, K.
Rajasekaran, J. Cary, and T.E. Cleveland
……………………………………………………………………………………….…51

Genetic Engineering of Cotton for Resistance to Aspergillus flavus and Other
Phytopathogens
J.W. Cary, K. Rajasekaran, M. Ulloa, J.M. Jaynes, and T.E. Cleveland
……………………………………………………………………………………….…52

Host Genetic Components for Aflatoxin Reduction Strategies in Peanut
Y. Chu, M.L. Ramos, P. Faustinelli, C. Holbrook, K. Rajasekaran, J. Cary, and P.
Ozias-Akins
…………………………………………………………………………………..…..…53

A Maize 9-Lipoxygenase Is Required for Resistance to Aflatoxin
Contamination, Insects, and Nematodes
X. Gao, T. Isakeit, J. Betrán, X. Ni, J. Starr, C. Göbel, M. Brodhagen, I. Feussner,
N. Keller, J. Engelberth, J. Tumlinson, and M. Kolomiets
…………………………………………………………………………………..…..…54

The Effect of PR-10 Expression on Host Resistance in Maize against Aspergillus
flavus Infection and Aflatoxin Production
Z.Y. Chen, R.L. Brown, K.E. Damann, and T.E. Cleveland
………………………………………………………………………………..……..…55

Annotation and Computational Analysis of Maize Proteome and Gene Expression
Datasets
S.M. Bridges, R. Kelley, G.B. Magee, N. Wang, S. Burgess, D.S. Luthe, and
W.P. Williams
…………………………………………………………………………………………56

PANEL DISCUSSION
Panel Chair: Peggy Ozias-Akins ………………………………………………….57

POSTERS PRESENTATIONS - None

CROP RESISTANCE - CONVENTIONAL BREEDING

Moderator – Paul Bertels, National Corn Growers

PLATFORM PRESENTATIONS

New Approaches to Breeding for Resistance to Preharvest Aflatoxin
Contamination in Peanut
C.C. Holbrook, E. Cantonwine, B.Z. Guo, P. Timper, D. Sullivan, and
D.M. Wilson
……………………………………………………………………………………...…58
PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
6
Variation Among Cultivars in Pod Water Uptake and Its Effect on Pre-harvest
Aflatoxin Contamination
D. Rowland, K. Gray, J. Dorner, S. Hilton, R. Sorensen, L. Powell, and C.
Holbrook
…………………………………………………………………………………………59

Remote Sensing for Rapid Selection of Drought and Aflatoxin Resistant Peanut
Genotypes
D. Sullivan and C. Holbrook
…………………………………………………………………………………………60

Development of Peanut EST (Expressed Sequence Tag)-based Genomic Resouces
and Tools
B.Z. Guo, H. Chen, P. Dang, M. Luo, X. Liang, G. He, C.C. Holbrook, and C.K.
Kvien
…………………………………………………………………………………………61

Field Based Assessment of Cotton Cultivars for Aflatoxin Contamination
M.W. Olsen and P.J. Cotty
…………………………………………………………………………………………62

The Development and Characterization of Navel Orangeworm and Aspergillus
flavus Resistance in Almond
T.M. Gradziel, G.H. McGranahan, and A. Dandekar
…………………………………………………………………………………………63

The Development of Aflatoxin-Resistant Maize Germplasm and the Identification
of Potential Markers
R.L. Brown, A. Menkir, R. Bandyopadhyay, Z.Y. Chen, and T.E. Cleveland
……………………………………………………………………………………….. .64

Microarray as a Tool for Understanding Mechanisms of Pre-harvest Aflatoxin
Contamination in Corn
M. Luo, J. Liu, R.D. Lee, and B. Guo
…………………………………………………………………………………………65

Diverse Maize Germplasm Aflatoxin Levels Survey
D. Bush, T. Musket, D. Davis, T. Brooks, W.P. Williams, G. Windham,
M. Krakowsky, and G. Davis
…………………………………………………………………………………………66

Improving Drought-Tolerance and Earworm Resistance to Reduce Aflatoxin in
Corn
W. Xu, G. Odvody, and W.P. Williams
…………………………………………………………………………………………67

Breeding Corn Germplasm for Agronomic Performance and Reduced
Aflatoxin Contamination
T. Isakeit, K. Mayfield, G. Odvody, and J. Betrán
…………………………………………………………………………………………68
PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
7
Searching for New Resistance to Reduce Aflatoxin in Corn
S.H. Moore, H.K. Abbas, and M.J. Millard
…………………………………………………………………………………………69

Creating Commercial Corn Hybrids with Low Aflatoxin in Grain
D. White
…………………………………………………………………………………………70

PANEL DISCUSSION
Panel Chair: Wenwei Xu, Leader …………………………………………….……71

POSTER PRESENTATIONS

Evaluation of Epidermal Conductance as a Potential Drought Tolerant Trait of
Peanut
E. Cantowine, S. Maddie, B. Buchanan, C.C. Holbrook, and C.K. Kvien
…………………………………………………………………………………………72

Genetic Response to Seed Colonization by Aspergillus flavus in Peanut
E. Cantowine1, C. C. Holbrook, D.M. Wilson, P. Songsri, S. Pimratch, and B.Z.
Guo
…………………………………………………………………………………………73

Production of Defensive Stilbenoids by Selected Peanut Genotypes
V.S. Sobolev, B.Z. Guo, C.C. Holbrook, and R.E. Lynch
…………………………………………………………………………………………74

Walnuts of Cultivar Tulare Show Similar Aflatoxin Contamination Levels as Those
of Two Other Cultivars
T.J. Michailides and M.A. Doster
…………………………………………………………………………………………75

Multi-location Evaluation of Aflatoxin Accumulation and Agronomic
Performance of Maize Hybrids in Texas
C. McKee, K. Mayfield, T. Isakeit, G. Odvody, and J. Betrán
…………………………………………………………………………………………76

Southern East Regional Aflatoxin Test (SERAT)
M. Krakowsky, B. Guo, D. White, W. Xu, W.P. Williams, T. Brooks, G. Windham,
S. Moore, H. Abbas, J. Perkins, D. Gorman, Q. Raab, K. Arnold, D. Smith, T.
Isakeit, K. Mayfield, and J. Betrán
…………………………………………………………………………………………77

Response to Aflatoxin of Exotic (CIMMYT and LAMA) Germplasm to Aflatoxin in
Southern USA
R. Corn, M. Krakowsky, W.P. Williams, D. Bergvinson, K. Mayfield, T. Isakeit, G.
Odvody, and J. Betrán
…………………………………………………………………………………………78

QTL Mapping for Response to Aflatoxin
H. Atta, A. Gutierrez, M. Menz, T. Isakeit, K. Mayfield, and J. Betrán
…………………………………………………………………………………..……79
PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
8
Jasmonic Acid Biosynthesis Pathway Confers Resistance to Maize Embryos
against Aspergillus flavus
A. Camas, Z. Magbanua, C. DeMoraes, J. Wilkinson, G. Windham, W.P.
Williams, and D.S. Luthe
……………………………………………………………………………………..…80

Is Rachis Lignification a Deterrent to Aspergillus flavus Movement through the
Developing Maize Ear?
L. Spangler, O. Pechanova, Z. Magbanua, W.P. Williams, and D.S. Luthe
…………………………………………………………………………………..……81

USE OF NATURAL PRODUCTS FOR PREVENTION OF FUNGAL INVASION
AND/OR AFLATOXIN BIOSYNTHESIS IN CROPS

Moderator – Howard Valentine, American Peanut Council

PLATFORM PRESENTATIONS

Aflatoxin Management in Arizona and Texas
P.J. Cotty and L. Antilla
…………………………………………………………………………………..……82

Persistence and Sporulation of an Aflatoxin Biocontrol Product Are
Influenced by Timing of Application
R. Jaime and P.J. Cotty
…………………………………………………………………………………..……83

Nonaflatoxigenic Aspergillus flavus TX9-8 Competitively Prevents Aflatoxin
Production by A. flavus Isolates of Large and Small Sclerotial Morphotypes
P.K. Chang and S.S.T. Hua
…………………………………………………………………………………..……84

Inhibition of Direct and Indirect Stress-Induced Aflatoxin Biosynthesis
R.J. Molyneux, N. Mahoney, B.C. Campbell, and J.H. Kim
……………………………………………………………………………………..…85

Maize Kernel Inhibitors of Aflatoxin Production in Aspergillus flavus
R. Holmes, R. Boston, and G.A. Payne
…………………………………………………………………………………..……86

PANEL DISCUSSION
Panel Chair: Peter Cotty………………………………………………………..….87

POSTER PRESENTATIONS - None

CROP MANAGEMENT AND HANDLING, INSECT CONTROL AND FUNGAL
RELATIONSHIPS

Moderator – Emory Murphy, Georgia Peanut Commission

PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
9
PLATFORM PRESENTATIONS

Examination of Genes Newly Introduced into Plants for Control of Corn Insects
Associated with Mycotoxin Problems
P.F. Dowd, E.T. Johnson, T.S. Pinkerton, R.W. Behle, and M.A. Berhow
…………………………………………………………………………………………89

Root-Knot Nematodes and Aflatoxin Contamination in Peanut
P. Timper and C.C. Holbrook
…………………………………………………………………………………………90

Furrow Diking for Improved Water Use Concerning Preharvest Aflatoxin
Contamination in Peanut and Corn
R.C. Nuti, J. Dorner, R. Sorenson, and M. Lamb
………………………………………………………………………….……………...91

Advances in Development of Kairomone – Augmented Control Tactics for Codling
Moth and Aspergillus in Walnuts
D.M. Light, J. Baker, and B.C. Campbell
……………………………………………………………………………….………...92

PANEL DISCUSSION
Panel Chair: Russell Nuti………………………………………….………………...93

POSTER PRESENTATIONS

Correlations between Biotic Stresses and Aflatoxin Contamination in Maize
M. Krakowsky, X. Ni, and R. Davis
………………………………………………………………………….………………95

Spatial Correlation between Aflatoxin Level and Ear-feeding Insect Damage in Pre-
harvest Corn
X. Ni, K. Da, M.D. Krakowsky, G.D. Buntin, R.D. Lee, and S.L. Brown
………………………………………………………………………….………………96

Effect of Glufosinate on Aflatoxin in Corn
S.H. Moore, H.K. Abbas, K.E. Damann, and H.J. Mascagni
……………………………………………………………………………….…………97

Effect of Exogenous Jasmonic Acid Application on Aspergillus flavus Kernel
Infection and Aflatoxin Production in Two Maize Hybrids (Zea mays L.)
L.K. Hawkins, D.S. Luthe, G.L. Windham, and W.P. Williams
……………………………………………………………………………….…………98

MICROBIAL ECOLOGY

Moderator – Roy Cantrell, Cotton Incorporated

PLATFORM PRESENTATIONS

PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006
10
Biological Control of Aspergillus flavus by Pichia anomala: Efficacy and Practical
Application
S.S.T. Hua
…………………………………………………………………………….……………99

Aflatoxin Control in Figs: Biocontrol using Atoxigenic Strains
M. Doster, T. Michailides, P. Cotty, D. Morgan, L. Boeckler, D. Felts, and H.
Reyes
………………………………………………………………………………...............100

Aflatoxin Control in Pistachios: Biocontrol using Atoxigenic Strains
M. Doster, T. Michailides, P. Cotty, D. Morgan, L. Boeckler, D. Felts, and H.
Reyes
…………………………………………………………………………….….............101

Potential for Reducing Aflatoxin in Corn with Field Application of a
Nontoxigenic Strain of Aspergillus flavus
J.W. Dorner
………………………………………………………………………….……………..102

Isolation of Bacterial Antagonists of Aspergillus flavus and Fusarium
verticillioides from Mississippi Corn Soils
J.D. Palumbo, T.L. O’Keefe, and H.K. Abbas
…………………………………………………………………………….…………..103

Studies on Aflatoxin Inhibition by Intra-specific “Competition”?
K.E. Damann, C. Huang, A. Jha, R. Sweany, and C. DeRobertis
………………………………………………………………………………..............104

Colonization and Aflatoxin Accumulation of Corn Following Pin Bar Inoculation
with Various Aspergillus flavus strains
H.K. Abbas, R. Zablotowicz, and H.A. Bruns
…………………………………………………………………………….…………..105

PANEL DISCUSSION
Panel Chair: Jeffery Palumbo……………………………………….……………..106

POSTER PRESENTATIONS

Antimicrobial Activity of Pyrrocidines from Acremonium zeae against
Endophytes and Pathogens of Maize
D.T. Wicklow and S.M. Poling
………………………………………………………………………….……………..107

Movement of Fusarium verticillioides between Inoculated and Non-inoculated Ears
in Field-grown Corn Plants
I.E. Yates and D. Sparks
………………………………………………………………………….……………..108

PROCEEDINGS OF THE 2006 ANNUAL MULTICROP AFLATOXIN/FUMONISIN ELIMINATION & FUNGAL
GENOMICS WORKSHOP · FT. WORTH, TX · OCTOBER 16-18, 2006