Plant Tissue Culture

223 Pages·2014·21.58 MB·English

New Frontiers in Plant In
Vitro Culture
Clive Koelling
New Frontiers in Plant In Vitro Culture
"This page is Intentionally Left Blank"
New Frontiers in Plant
In Vitro Culture
Edited by Clive Koelling
Published by Academic Pages,
5 Penn Plaza,
19th Floor,
New York, NY 10001, USA
New Frontiers in Plant In Vitro Culture
Edited by Clive Koelling
© 2017 Academic Pages
International Standard Book Number: 978-1-9789-2718-6
This book contains information obtained from authentic and highly regarded sources. Copy-
right for all individual chapters remain with the respective authors as indicated. A wide
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Contents
Preface VII
Chapter 1 Plant Tissue Culture: Current Status and Opportunities 1
Altaf Hussain, Iqbal Ahmed Qarshi,
Hummera Nazir and Ikram Ullah
Chapter 2 Plant Tissue Culture Media 29
Abobkar I.M. Saad and Ahmed M. Elshahed
Chapter 3 The Prerequisite of the Success in Plant Tissue Culture:
High Frequency Shoot Regeneration 41
Mustafa Yildiz
Chapter 4 In vitro Tissue Culture, a Tool for the Study and Breeding
of Plants Subjected to Abiotic Stress Conditions 69
Rosa Mª Pérez-Clemente and Aurelio Gómez-Cadenas
Chapter 5 The Science of Plant Tissue Culture as
a Catalyst for Agricultural and Industrial
Development in an Emerging Economy 87
S.E. Aladele, A.U. Okere, E. Jamaldinne , P.T. Lyam,
O. Fajimi, A. Adegeye and C.M. Zayas
Chapter 6 Somaclonal Variation in Tissue Culture:
A Case Study with Olive 109
A.R. Leva, R. Petruccelli and L.M.R. Rinaldi
Chapter 7 Flow Cytometry Applied in Tissue Culture 137
Moacir Pasqual, Leila Aparecida Salles Pio,
Ana Catarina Lima Oliveira and
Joyce Dória Rodrigues Soares
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VI Contents
Chapter 8 Polyamines, Gelling Agents in Tissue Culture,
Micropropagation of Medicinal Plants and Bioreactors 151
Giuseppina Pace Pereira Lima, Renê Arnoux da Silva Campos,
Lilia Gomes Willadino, Terezinha J.R. Câmara and Fabio Vianello
Chapter 9 Tissue Culture Techniques for
Native Amazonian Fruit Trees 169
Moacir Pasqual, Edvan Alves Chagas,
Joyce Dória Rodrigues Soares and Filipe Almendagna Rodrigues
Chapter 10 Effect of Additives on Micropropagation of an
Endangered Medicinal Tree Oroxylum indicum L. Vent 183
Y.K. Bansal and Mamta Gokhale
Chapter 11 Production of Useful Secondary Metabolites Through
Regulation of Biosynthetic Pathway in Cell and
Tissue Suspension Culture of Medicinal Plants 197
Hu Gaosheng and Jia Jingming
Permissions
List of Contributors
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Preface
Plant in vitro culture forms an essential part in the field of botany. This book serves
the objective of presenting recent developments in the field of plant in vitro culture
in relation with medicinal plants and perennial fruit crops. It extensively covers
fundamental principles and recent techniques. It includes contributions of eminent
researchers and experts in this field from all over the world. This book will serve
as a valuable reference for students, teachers, researchers in biotechnology and for
individuals interested in commercial applications of plant in vitro culture.
This book unites the global concepts and researches in an organized manner for a
comprehensive understanding of the subject. It is a ripe text for all researchers, students,
scientists or anyone else who is interested in acquiring a better knowledge of this
dynamic field.
I extend my sincere thanks to the contributors for such eloquent research chapters.
Finally, I thank my family for being a source of support and help.
Editor
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Chapter 1
Plant Tissue Culture:
Current Status and Opportunities
Altaf Hussain, Iqbal Ahmed Qarshi, Hummera Nazir and Ikram Ullah
Additional information is available at the end of the chapter

1. Introduction
Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant under
controlled nutritional and environmental conditions [1] often to produce the clones of
plants. The resultant clones are true-to type of the selected genotype. The controlled
conditions provide the culture an environment conducive for their growth and
multiplication. These conditions include proper supply of nutrients, pH medium, adequate
temperature and proper gaseous and liquid environment.
Plant tissue culture technology is being widely used for large scale plant multiplication.
Apart from their use as a tool of research, plant tissue culture techniques have in recent
years, become of major industrial importance in the area of plant propagation, disease
elimination, plant improvement and production of secondary metabolites. Small pieces of
tissue (named explants) can be used to produce hundreds and thousands of plants in a
continuous process. A single explant can be multiplied into several thousand plants in
relatively short time period and space under controlled conditions, irrespective of the
season and weather on a year round basis [2]. Endangered, threatened and rare species have
successfully been grown and conserved by micropropagation because of high coefficient of
multiplication and small demands on number of initial plants and space.
In addition, plant tissue culture is considered to be the most efficient technology for crop
improvement by the production of somaclonal and gametoclonal variants. The
micropropagation technology has a vast potential to produce plants of superior quality,
isolation of useful variants in well-adapted high yielding genotypes with better disease
resistance and stress tolerance capacities [3]. Certain type of callus cultures give rise to
clones that have inheritable characteristics different from those of parent plants due to
the possibility of occurrence of somaclonal variability [4], which leads to the
development of commercially important improved varieties. Commercial production of

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