Plant Protoplasts and Genetic Engineering V

431 Pages·1994·11.528 MB·English

by  Y. H. Dan, C. T. Stephens (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

Vo lumes alread y published
Volume 1: Trees I (1986)
Volume 2: Crops 1(1986)
Volume 3: Potato (1987)
Volume 4: Medicinal and Aromatic Plants I (1988)
Volume 5: Trees 11 (1989)
Volume 6: Crops 11 (1988)
Volume 7: Medicinal and Aromatic Plants 11 (1989)
Volume 8: Plant Protoplasts and Genetic Engineering I (1989)
Volume 9: Plant Protoplasts and Genetic Engineering 11 (1989)
Volume 10: Legurnes and Oilseed Crops I (1990)
Volume 11: Somaclonal Variation in Crop Improvement I (1990)
Volume 12: Haploids in Crop Improvement I (1990)
Volume 13: Wheat (1990)
Volume 14: Rice (1991)
Volume 15: Medicinal and Aromatic Plants III (1991)
Volume 16: Trees III (1991)
Volume 17: High-Tech and Micropropagation I (1991)
Volume 18: High-Tech and Micropropagation 11 (1992)
Volume 19: High-Tech and Micropropagation III (1992)
Volume 20: High-Tech and Micropropagation IV (1992)
Volume 21: Medicinal and Aromatic Plants IV (1993)
Volume 22: Plant Protoplasts and Genetic Engineering III (1993)
Volume 23: Plant Protoplasts and Genetic Engineering IV (1993)
Volume 24: Medicinal and Aromatic Plants V (1993)
Volume 25: Maize (1994)
Volume 26: Medicinal and Aromatic Plants VI (1994)
Volume 27: Somatic Hybridization in Crop Improvement I (1994)
Volume 28: Medicinal and Aromatic Plants VII (1994)
Volume 29: Plant Protoplasts and Genetic Engineering V (1994)
Volumes in preparation
Volume 30: Somatic Embryogenesis and Synthetic Seed I (1995)
Volume 31: Somatic Enbryogenesis and Synthetic Seed 11 (1995)
Volume 32: Cryopreservation of Plant Germplasm I (1995)
Volume 33: Medicinal and Aromatic Plants VIII
Volume 34: Plant Protoplasts and Genetic Engineering VI
Volume 35: Trees IV
Volume 36: Somaclonal Variation in Crop Improvement 11
Volume 37: Medicinal and Aromatic Plants VIII
Volume 38: Plant Protoplasts and Genetic Engineering VII
Biotechnology in
Agriculture and Forestry 29
Plant Protoplasts
and Genetic Engineering V
Edited by Y.P.s. Bajaj
With 148 Figures
Springer-Verlag Berlin Heide1berg GmbH
Professor Dr. YP.S. BAJAJ
A-137
New Friends Colony
New Delhi 110065, India
ISBN 978-3-642-08182-8
Library of Congress Cataloging-in-Publication Data (Revised for vol. 5) Plant protoplasts and genetic
engineering. (Biotechnology in agriculture and forestry; 8 9, 22 23, 29) Includes bibliographies and
indexes. I. Plant protoplasts. 2. Plant geneticengineering. I. Bajaj, V.P.S., 1936 .11. Series. QK725.P578
1989,581.87'3 88-31197
ISBN 978-3-642-08182-8 ISBN 978-3-662-09366-5 (eBook)
DOI 10.1007/978-3-662-09366-5
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© Springer-Verlag Berlin Heidelberg 1994
Origina1ly published by Springer-Verlag Berlin Heidelberg New York in 1994
Softcover reprint of tbe hardcover 1st edition 1994
The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply,
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Dedicated to
Professor Dr. A. van Kammen
with whom I had the privilege of working
at the Department of Molecular Biology,
Agricultural University Wageningen,
The Netherlands, during 1973-1974
Preface
Plant protoplasts have proved to be an excellent tool for in vitro
manipulations, somatic hybridization, DNA uptake and genetic trans
formation, and for the induction of somaclonal variation. These studies
reflect the far-reaching impact of protoplast alterations on agriculture and
forest biotechnology. Taking these aspects into consideration, the series of
books on Plant Protoplasts and Genetic Engineering provides a survey of
the literature, focusing on recent information and the state of the art in
protoplast manipulation and genetic transformation. This book, Plant
Protoplasts and Genetic Manipulation V, like the previous four volumes
published in 1989 and 1993, is unique in its approach. It comprises 27
chapters dealing with the regeneration of plants from protoplasts, and
genetic transformation in various species of Actinidia, Allocasuarina,
Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina,
Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron,
Malus, Musa, Physalis, Physcomitrella, Picea, Rosa, Tagetes, Triticum, and
Ulmus.This book may be of special interest to advanced students, teachers
and research scientists in the field of plant tissue culture, molecular
biology, genetic engineering, plant breeding, and general biotechnology.
New Delhi, September 1994 Professor Y.P.S. BAJAJ
Series Editor
Contents
Section I Regeneration of Plants from Protoplasts .
I.l Regeneration of Plants from Protoplasts
of Asparagus officinalis L.
Y. H. DAN and C. T. STEPHENS (With 3 Figures)
1 Introduction. . . . . . 3
2 Isolation of Protoplasts 5
3 Culture of Protoplasts . 7
4 Plant Regeneration of Calli from Single Protoplasts. 12
5 Summary and Conclusions . 13
6 Protocol. 14
References . . . . . . . . . . 15
I.2 Regeneration of Plants from Protoplasts
of Beta vulgaris (Sugar Beet)
R. D. HALL, C. PEDERSEN, and F. A. KRENS (With 4 Figures)
1 Introduction. . . . . . . . . . . . . . . . 16
2 Isolation of Protoplasts . . . . . . . . . . . . . . . . . 18
3 Protoplast Culture and Plant Regeneration. . . . . . . . 24
4 Protoplast Pretreatment and Protoplast Fusion Techniques . 29
5 Miscellaneous Studies on Protoplasts. 32
6 Protocol. . 34
7 Conclusions 34
References . . 35
1.3 Regeneration of Plants from Protoplasts of Brassica juncea (L.)
Czern & Coss (Brown Mustard)
E.-C. PUA (With 2 Figures)
Introduction. . . . . . 38
2 Isolation of Protoplasts 41
3 Culture of Protoplasts . 41
4 Plant Regeneration and Acclimatization 42
5 Transgene Expression During Plant Regeneration
from Protoplasts of B. juncea Via Somatic Embryogenesis . .. 45
x
Contents
6 Fusion of Protoplasts . . . . . . . . 46
7 Conclusions and Prospects. . . . . . 46
8 Protocol for Plant Regeneration from
Hypocotyl-Derived Protoplasts
of var. Indian Mustard Via Somatic Embryogenesis . 47
References . . . . . . . . . . . . . . . . . . . . . . 48
1.4 Regeneration of Plants from Protoplasts
of Carica Species (Papaya)
M. H. CHEN (With 1 Figure)
1 Introduction. . . . . . 52
2 Isolation of Protoplasts 53
3 Culture of Protoplasts . 55
4 Regeneration of Plants . 57
5 Fusion of Protoplasts 58
6 Conclusion 58
7 Protocols 58
References . . 59
1.5 Regeneration of Plants from Protoplasts
of Larix Species (Lareh)
P. J. CHAREST and K. KLIMASZEWSKA (With 3 Figures)
1 General Account. . . . . . . . . . . . . . . . . . 61
2 Establishing an Embryogenie Suspension. . . . . . 67
3 Protoplast Isolation from Embryogenie Suspensions 67
4 Culture of Protoplasts . . . . . . . . . 68
5 Regeneration of Trees from Protoplasts. 69
6 Summary 69
7 Protocol. 69
References . 70
I.6 Regeneration of Plants from Protoplasts
of Statice (Limonium perezii Hubbard)
H. KUNITAKE and M. Mn (With 3 Figures)
1 Introduction . . . . . . 72
2 Isolation of Protoplasts 73
3 Culture of Protoplasts . 73
4 Regeneration of Plants . 76
5 Conclusions and Prospects . 80
6 Protocol. 80
References . . . . . . . . . . 81
Contents XI
1.7 Regeneration of Plants from Protoplasts
of Malus Xdomestica Borkh. (Apple)
E. M. PATAT-ÜCHATT (With 3 Figures)
1 Introduction. . . . . . . . . . . . 83
2 Isolation of Protoplasts . . . . . . 85
3 Protoplast Culture and Callus Production 90
4 Plant Regeneration from Protoplast-Derived Callus . 93
5 Miscellaneous Studies 97
6 Conclusion 99
References . . . . . . . 99
1.8 Regeneration of Plants from Protoplasts
of Musa Species (Banana)
B. PANIS, L. SAGI, and R. SWENNEN (With 6 Figures)
Introduction. . . . . . . . . . . . . . . . . . 102
2 Review on Banana Cell and Protoplast Culture . 102
3 Isolation of Protoplasts 104
4 Culture of Protoplasts . 107
5 Regeneration of Plants . 110
6 Genetic Transformation 112
7 Conclusion and Prospects 112
References . . . . . . . . . 113
1.9 Regeneration of Plants from Protoplasts
of Picea Species (Spruce)
L. TREMBLAY and F. M. TREMBLAY (With 12 Figures)
1 Introduction. . . . . . 115
2 Isolation of Protoplasts 116
3 Culture of Protoplasts . 121
4 Regeneration of Plants . 126
5 Fusion of Protoplasts 126
6 Miscellaneous Studies 127
7 Summary 127
8 Protocol. 128
References . 128
1.10 Regeneration of Plants from Protoplasts of Physalis Species
J. A. EASH and A. C. W AISS, Jr. (With 7 Figures)
1 Introduction. . . . . . 131
2 Isolation of Protoplasts 133
3 Culture of Protoplasts . 135
4 Regeneration of Plants . 139
XII Contents
5 Fusion of Protoplasts 141
6 Conclusions and Prospects . 141
7 Protocol. 142
References . . . . . . . . . . 144
1.11 Regeneration of Plants from Protoplasts
of Rosa Species (Roses)
D. MATTHEWS, J. MOTTLEY, K. YOKOYA, and A. V. ROBERTs
(With 4 Figures)
1 Introduction . . . . . . 146
2 Isolation of Protoplasts 147
3 Protoplast Culture. . . 151
4 Regeneration of Plants . 154
5 Genetic Transformation of Protoplasts 156
6 Conclusions and Prospects . 156
7 Protocols 157
References . . . . . . . . . . 159
1.12 Regeneration of Plants from Protoplasts
of Triticum aestivum L. (Wheat)
Y.-F CHANG and J. R. WONG (With 13 Figures)
1 Introduction. . . . . . 161
2 Isolation of Protoplasts 162
3 Culture of Protoplasts . 166
4 Regeneration of Plants . 167
5 Conclusion 168
6 Protocol. 168
References . . 170
1.13 Regeneration of Plants from Protoplasts
of V/mus Species (Elms)
N. DORION, H. BEN JOUIRA, P. DANTHU, and C. BIGOT
(With 9 Figures)
1 Introduction. . . . 172
2 Protoplast Isolation 175
3 Protoplast Culture . 181
4 Plant Regeneration 185
5 Conclusions and Perspectives 186
6 Protocol. 186
References . . . . . . . . . . . 188
Section 11 Genetic Transformation and Transgenie Plants . . 191