EVALUATION OF GREENGRAM GENOTYPES UNDER IMPOSED MOISTURE STRESS CONDITIONS M. DEEPA B.Sc., (Ag) MASTER OF SCIENCE IN AGRICULTURE (PLANT PHYSIOLOGY) 2011 EVALUATION OF GREENGRAM GENOTYPES UNDER IMPOSED MOISTURE STRESS CONDITIONS BY M. DEEPA B.Sc., (Ag) THESIS SUBMITTED TO THE ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN AGRICULTURE (PLANT PHYSIOLOGY) CHAIRPERSON: Dr. G. RAMA RAO DEPARTMENT OF PLANT PHYSIOLOGY SRI VENKATESWARA AGRICULTURAL COLLEGE, TIRUPATI ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD – 500 030 2011 DECLARATION I, Ms. M. DEEPA, hereby declare that the thesis entitled “EVALUATION OF GREENGRAM GENOTYPES UNDER IMPOSED MOISTURE STRESS CONDITIONS” submitted to Acharya N. G. Ranga Agricultural University, Hyderabad for the award of degree of MASTER OF SCIENCE IN AGRICULTURE is the result of original research work done by me. I also declare that no material contained in this thesis has been published earlier in any manner. Place : (M. DEEPA) I.D. No- TAM-2009/34 Date : ii CERTIFICATE This is to certify that the thesis entitled “EVALUATION OF GREENGRAM GENOTYPES UNDER IMPOSED MOISTURE STRESS CONDITIONS” submitted in partial fulfillment of the requirements for the award of degree of MASTER OF SCIENCE (Agriculture) in PLANT PHYSIOLOGY to the Acharya N.G. Ranga Agricultural University, Hyderabad, is a record of the bonafide research work carried out by Ms. M. DEEPA under our guidance and supervision. The subject of the thesis has been approved by the Student’s Advisory Committee. No part of the thesis has been submitted by the student for the award of any other degree or diploma. The published part has been fully acknowledged. All assistance and help received during the course of the investigations have been duly acknowledged by the author of the thesis. (Dr. G. RAMA RAO) Chairperson of the Advisory Committee Thesis approved by the Student’s Advisory Committee: Chairperson : Dr. G. RAMA RAO _______________ Associate Professor Department of Plant Physiology S.V. Agricultural College, Tirupati – 517 502, A.P. Member : Dr. P. LATHA _______________ Scientist Department of Plant Physiology Institute of Frontier Technology Regional Agricultural Research Station Tirupati – 517 502, A.P. Member : Dr. M.V.S. NAIDU _______________ Associate Professor Dept. of Soil Science and Agricultural Chemistry S.V. Agricultural College Tirupati – 517 502, A.P. Date of final viva-voce: iii CERTIFICATE Ms. M. DEEPA has satisfactorily prosecuted the course of research and that the thesis entitled “EVALUATION OF GREENGRAM GENOTYPES UNDER IMPOSED MOISTURE STRESS CONDITIONS” submitted is the result of original research work and is of sufficiently high standard to warrant its presentation to the examination. I also certify that neither the thesis nor its part there of has not been previously submitted by her for a degree of any university. (Dr. G. RAMA RAO) Chairperson Associate Professor Department of Plant Physilogy S. V. Agricultural College Tirupati – 517 502. Date : iv ACKNOWLEDGEMENTS I am ineffable to express my esteemed thanks to the revered chairman of my advisory committee Dr. G. Rama Rao, Associate Professor, Department of Plant Physiology, S.V. Agricultural College, Tirupati for her keen interest, ardent support and persistent encouragement showered to me. I am inexpressibly ecstatic to extend my deep sense of my gratitude to luminous educationalist and Dr. P. Sudhakar, Senior Scientist, Department of Plant Physiology, Institute of Frontier Technology, Regional Agricultural Research Station, Tirupati for his dexterous guidance, illuminating suggestions and unremitting assistance throughout the period of study, research and in completion of this thesis. I sincerely accentuate my everlasting gratitude to the revered member of my advisory committee Dr. P. Latha, Scientist, Department of Plant Physiology, Institute of Frontier Technology, Regional Agricultural Research Station, Tirupati and Dr. M.V.S. Naidu, Associate Professor, Department of Soil Science and Agricultural Chemistry for their inspiring, meticulous and valuable guidance during the entire period of my investigation. It is my honour to portray Dr. K. Balakrishna Reddy, Professor, Department of Plant Physiology, S.V. Agricultural College, Mrs. Dr. V. Raja Rajeswari, Professor & Head, Department of Plant Physiology for their valuable suggestions and persistent encouragement during the course of this study. In explicable in fervent to my parents Smt. M. Tulasamma and Sri. M. Pulla Reddy and, my loving brother M. Niranjan Reddy for their undiminishing love, benign care and zealous encouragement throughout this endeavour. v Special thanks to my dear friend K. Sujata, Technical Assistant, Institute of Frontier Technology, RARS, Tirupati for her help, friendly assistance and valuable cooperation during my research work. With utmost satisfaction I acknowledge the enormous help of my colleagues Sagarika, Dilip, Pranusha, Isha, Ramadevi, Indumathi, Swetha, Ashok, Sivasankar, Pande, Sandeep, Subbaiah, Subhakar and Somu and juniors Rajasekhar, Raghavendra, Sarat, Geeta, Rama, Sharmila and Jayaramudu for their friendly assistance and valuable cooperation during my research work. I am thankful to K. Bhanu Priya, Teaching Assistant in MABM and C. Lalitha, for their assistance during my research work. I am grateful to Acharya N.G. Ranga Agricultural University, Hyderabad for providing me with the opportunity for prosecuting my post graduation programme and also the facilities and financial assistance provided during my study. I sincerely thank the farming community who helped me in successful completion of this humble piece of work. In finale, I thank all my well wishers and others who helped me directly or indirectly not placed here, for their kind cooperation and support rendered to me. vi CONTENTS Chapter No. Title Page No. I INTRODUCTION 1-3 II REVIEW OF LITERATURE 4-18 III MATERIAL AND METHODS 19-33 IV RESULTS AND DISCUSSION 34-89 V SUMMARY AND CONCLUSIONS 90-93 LITERATURE CITED 94-102 vii LIST OF TABLES Table Page Title No. No. Mechanical analysis of soil experimental plots (Bouyoucos 3.1 20 hydrometer method, Piper, 1950) 3.2 Chemical analysis of soil experimental plots 20 Standard week wise meteorological data during the crop growth 3.3 22 period of groundnut (07-01-2011 to 23-03-2011) Effect of mid season and end season moisture stress on field 4.1 35 emergence index of green gram genotypes 4.2 Effect of mid season and end season moisture stress on days 37 to 50% flowering of green gram genotypes 4.3 Effect of mid season and end season moisture stress on 39-40 plant height (cm) of greengram genotypes 4.4 Effect of mid season and end season moisture stress on root 42-43 biomass (g plant-1) of green gram genotypes 4.5 Effect of mid season and end season moisture stress on 44-45 shoot biomass (g plant-1) of green gram genotypes 4.6 Effect of mid season and end season moisture stress on leaf 47-48 biomass (g plant-1) of green gram genotypes 4.7 Effect of mid season and end season moisture stress on pod 49 dry weight (g plant-1)of green gram genotypes 4.8 Effect of mid season and end season moisture stress on total 52-53 dry matter (g plant-1) of green gram genotypes 4.9 Effect of mid season and end season moisture stress on leaf 55-56 area (cm2 plant-1) of greengram genotypes 4.10 Effect of mid season and end season moisture stress on leaf 58-59 area index of greengram genotypes viii 4.11 Effect of mid season and end season moisture stress on crop 61-62 growth rate (g m-2 day-1) of greengram genotypes 4.12 Effect of mid season and end season moisture stress on net 64-65 assimilation rate (g dm-2 day-1) of greengram genotypes 4.13 Effect of mid season and end season moisture stress on leaf 67-68 area duration (cm2day-1) of green gram genotypes 4.14 Effect of mid season and end season moisture stress on 70 photosynthetic rate (mol CO m-2 sec-1) of green gram 2 genotypes 4.15 Effect of mid season and end season moisture stress on 71 transpiration rate (m mol H O m-2 sec-1) of green gram 2 genotypes 4.16 Effect of mid season and end season moisture stress on 73 stomatal conductance (mol H O m-2 sec-1) of green gram 2 genotypes 4.17 Effect of mid season and end season moisture stress on 75 Specific leaf area (cm2 g-1) of green gram genotypes 4.18 Effect of mid season and end season moisture stress on SPAD 76 chlorophyll meter reading of green gram genotypes 4.19 Effect of mid season and end season moisture stress on 79 chlorophyll fluorescence (Fv/Fm) of green gram genotypes 4.20 Effect of mid season and end season moisture stress on 83 yield components of green gram genotypes 4.21 Effect of mid season and end season moisture stress on 87 yield and harvest index of green gram genotypes ix
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